Rorug02G0116500

Prolyl oligopeptidase, N-terminal beta-propeller domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0018607
Physical Location & Seq
Reverse (-)
9891538 .. 9894486
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0116500.1

Sequence Viewer

Length: 993 bp
ATGATGCTCGGAGCTGCCCAATTCTCCGTCGCCGATCATCGCTCGAATGCCGTTTTTCCGATCCCAAATACTCGGCCAGCTGTTTCTTCTTCCTCTTCTCCTCACACCTCCTCCGATTTCGTCGTCGTTCGGTTAGGTTTCAGACCCAAACGCCGCCACCGTGGACTCAAATCGATCCGCATTACCTGCAACTCTTCCCCTCACGACAGATCCCTTGATTCCGATCATCAAAACTACGAGTATATCCAAGCTTCCCTCTTGCTTTCAGAAACTATATCTCACTACCGTATGCAAAAGAGAGGATTTCAAGAAGAGACGAATTGGAATTCCTCCGGTAAATTGCGGCCATTTTCGGTCCGAGCCACCCGCCCCCGGAATGTTTTGGATACACTAGACAGGAACTTTCTCCACCGTTTCCAAACTCCTACCATTTTCCTCAAGATTTCGTGTGACGGAGACTTTTTGCTTCCAATTGTTGTAGGCGAACTTGCGATAGAGAATCTCATAAATGGCCACTGGGGAGATGAATTTGGGGATTCCCCAGACCAGTTTCAGTTTGTGAAAAATCTTGTCGAAAAACTTGACCATCAAGTGAATATGGTGAGAATTACAGAAAGAGTGCTGAATACTTACTTTGCCAGATTGTATTTTAGCAAGGTGGTGGTGCCAGGCAAAAGTGACATTCTTAGTGTGGATGCACGTCCATCAGATGTCATAAATGTTGCATATAGATGCAAGGCACCCATATATGTAAATAAACAGATTGTTTTAAAAGATGCTGTAAGAATCAGTTATGGGATGGGCAGAATGCGTGATATGAAGTCTACTTACGATGTCTCTCTTGATAGTGCTGCAGATGGTCCCAATTTTTTAGCTCAAGAACTTGTTTTGGTGAAGAAGATCAAATTAGCTATCAAAGAGGAAAGATATAAGGATGCAGCCATGTGGAGAGACGAACTAGTCAAACTTCGCAAGTTAAGTCAAGGACATTAA

Protein Analysis

330

Amino Acids

37.7

Weight (kDa)

9.6

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 152 - 262 4.9e-17 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 959
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 664, 739
AccI GTMKAC 1 cut(s) 824
AciI CCGC 4 cut(s) 154, 178, 343, 367
AclWI GGATC 3 cut(s) 55, 169, 204
AcoI YGGCCR 3 cut(s) 74, 344, 511
AcsI RAATTY 2 cut(s) 325, 527
AfiI CCNNNNNNNGG 1 cut(s) 372
AgsI TTSAA 1 cut(s) 308
AhlI ACTAGT 1 cut(s) 958
AjiI CACGTC 1 cut(s) 701
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 5 cut(s) 14, 80, 251, 875, 911
AluI AGCT 5 cut(s) 14, 80, 251, 875, 911
Alw26I GTCTC 4 cut(s) 308, 450, 841, 945
AlwI GGATC 3 cut(s) 55, 169, 204
AoxI GGCC 3 cut(s) 74, 344, 511
ApeKI GCWGC 3 cut(s) 14, 851, 938
ApoI RAATTY 2 cut(s) 325, 527
AspS9I GGNCC 2 cut(s) 355, 860
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 2 cut(s) 613, 904
AvaII GGWCC 2 cut(s) 355, 860
BalI TGGCCA 1 cut(s) 513
BanI GGYRCC 2 cut(s) 664, 739
BarI GAAGNNNNNNTAC 2 cut(s) 812, 844
BbvI GCAGC 2 cut(s) 838, 950
BccI CCATC 4 cut(s) 594, 712, 793, 851
BceAI ACGGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 669
BciVI GTATCC 1 cut(s) 379
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 4 cut(s) 308, 450, 841, 945
BcuI ACTAGT 1 cut(s) 958
BfaI CTAG 2 cut(s) 392, 959
BfmI CTRYAG 1 cut(s) 852
BfuAI ACCTGC 1 cut(s) 194
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 5 cut(s) 15, 154, 344, 852, 939
BlsI GCNGC 5 cut(s) 16, 155, 345, 853, 940
Bme1390I CCNGG 2 cut(s) 373, 669
Bme18I GGWCC 2 cut(s) 355, 860
BmgBI CACGTC 1 cut(s) 701
BmgT120I GGNCC 2 cut(s) 355, 860
BmiI GGNNCC 3 cut(s) 666, 741, 862
BmrFI CCNGG 2 cut(s) 373, 669
BmrI ACTGGG 1 cut(s) 526
BmsI GCATC 4 cut(s) 685, 722, 766, 925
BmuI ACTGGG 1 cut(s) 526
BpuEI CTTGAG 2 cut(s) 422, 861
BpuMI CCSGG 1 cut(s) 373
Bsa29I ATCGAT 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 160, 371
BsaWI WCCGGW 1 cut(s) 332
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 1 cut(s) 372
Bse1I ACTGG 2 cut(s) 521, 547
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 1 cut(s) 669
BseCI ATCGAT 1 cut(s) 173
BseDI CCNNGG 2 cut(s) 160, 371
BseGI GGATG 3 cut(s) 700, 804, 940
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 372
BseNI ACTGG 2 cut(s) 521, 547
BseRI GAGGAG 2 cut(s) 90, 100
BseXI GCAGC 2 cut(s) 838, 950
BshFI GGCC 3 cut(s) 76, 346, 513
BshNI GGYRCC 2 cut(s) 664, 739
BshVI ATCGAT 1 cut(s) 173
BsiSI CCGG 2 cut(s) 333, 373
BslFI GGGAC 1 cut(s) 846
BslI CCNNNNNNNGG 1 cut(s) 372
BsmAI GTCTC 4 cut(s) 308, 450, 841, 945
BsmBI CGTCTC 2 cut(s) 308, 945
BsmFI GGGAC 1 cut(s) 846
BsmI GAATGC 2 cut(s) 52, 813
BsnI GGCC 3 cut(s) 76, 346, 513
Bsp143I GATC 6 cut(s) 34, 60, 174, 209, 223, 900
BspACI CCGC 4 cut(s) 154, 178, 343, 367
BspANI GGCC 3 cut(s) 76, 346, 513
BspDI ATCGAT 1 cut(s) 173
BspLI GGNNCC 3 cut(s) 666, 741, 862
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 3 cut(s) 55, 169, 204
BspT107I GGYRCC 2 cut(s) 664, 739
BsrI ACTGG 2 cut(s) 521, 547
BssECI CCNNGG 2 cut(s) 160, 371
BssMI GATC 6 cut(s) 34, 60, 174, 209, 223, 900
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 3 cut(s) 161, 287, 413
Bst6I CTCTTC 3 cut(s) 100, 199, 306
BstAPI GCANNNNNTGC 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 686
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 3 cut(s) 700, 804, 940
BstKTI GATC 6 cut(s) 37, 63, 177, 212, 226, 903
BstMAI GTCTC 4 cut(s) 308, 450, 841, 945
BstMBI GATC 6 cut(s) 34, 60, 174, 209, 223, 900
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNI CCWGG 1 cut(s) 669
BstSCI CCNGG 2 cut(s) 371, 667
BstSFI CTRYAG 1 cut(s) 852
BstV1I GCAGC 2 cut(s) 838, 950
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 173
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 3 cut(s) 76, 346, 513
BsuTUI ATCGAT 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 701
BtsCI GGATG 3 cut(s) 700, 804, 940
BtsIMutI CAGTG 1 cut(s) 514
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 78
Cfr13I GGNCC 2 cut(s) 355, 860
ClaI ATCGAT 1 cut(s) 173
CpoI CGGWCCG 1 cut(s) 355
CspI CGGWCCG 1 cut(s) 355
CviAII CATG 1 cut(s) 943
DdeI CTNAG 1 cut(s) 686
DpnI GATC 6 cut(s) 36, 62, 176, 211, 225, 902
DpnII GATC 6 cut(s) 34, 60, 174, 209, 223, 900
DraI TTTAAA 1 cut(s) 771
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
EaeI YGGCCR 3 cut(s) 74, 344, 511
Eam1104I CTCTTC 3 cut(s) 100, 199, 306
EarI CTCTTC 3 cut(s) 100, 199, 306
Eco47I GGWCC 2 cut(s) 355, 860
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 667
Esp3I CGTCTC 2 cut(s) 308, 945
FaeI CATG 1 cut(s) 946
FaqI GGGAC 1 cut(s) 846
FatI CATG 1 cut(s) 942
FauI CCCGC 1 cut(s) 374
FblI GTMKAC 1 cut(s) 824
Fnu4HI GCNGC 5 cut(s) 15, 154, 344, 852, 939
FokI GGATG 3 cut(s) 707, 811, 947
Fsp4HI GCNGC 5 cut(s) 15, 154, 344, 852, 939
FspBI CTAG 2 cut(s) 392, 959
GluI GCNGC 5 cut(s) 15, 154, 344, 852, 939
HaeIII GGCC 3 cut(s) 76, 346, 513
HapII CCGG 2 cut(s) 333, 373
Hin1II CATG 1 cut(s) 946
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 5 cut(s) 165, 218, 499, 536, 786
HpaII CCGG 2 cut(s) 333, 373
HphI GGTGA 2 cut(s) 613, 904
Hpy166II GTNNAC 2 cut(s) 164, 825
Hpy188I TCNGA 8 cut(s) 11, 60, 115, 143, 223, 268, 359, 709
Hpy188III TCNNGA 5 cut(s) 203, 308, 439, 842, 878
Hpy8I GTNNAC 2 cut(s) 164, 825
Hpy99I CGWCG 3 cut(s) 32, 125, 128
HpyCH4III ACNGT 3 cut(s) 161, 287, 413
HpyCH4IV ACGT 1 cut(s) 700
HpyCH4V TGCA 7 cut(s) 189, 292, 698, 725, 735, 854, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 1 cut(s) 686
HpySE526I ACGT 1 cut(s) 700
Hsp92II CATG 1 cut(s) 946
Kzo9I GATC 6 cut(s) 34, 60, 174, 209, 223, 900
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 2 cut(s) 838, 950
LweI GCATC 4 cut(s) 685, 722, 766, 925
MaeI CTAG 2 cut(s) 392, 959
MaeII ACGT 1 cut(s) 700
MaeIII GTNAC 2 cut(s) 449, 677
MalI GATC 6 cut(s) 36, 62, 176, 211, 225, 902
MboI GATC 6 cut(s) 34, 60, 174, 209, 223, 900
MboII GAAGA 7 cut(s) 78, 81, 87, 186, 323, 907, 910
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 209
MlsI TGGCCA 1 cut(s) 513
MluCI AATT 9 cut(s) 20, 319, 325, 338, 471, 527, 606, 865, 905
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 1 cut(s) 159
Mox20I TGGCCA 1 cut(s) 513
MscI TGGCCA 1 cut(s) 513
MseI TTAA 3 cut(s) 770, 977, 991
MslI CAYNNNNRTG 1 cut(s) 730
Msp20I TGGCCA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 2 cut(s) 333, 373
MspR9I CCNGG 2 cut(s) 373, 669
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 2 cut(s) 52, 813
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 1 cut(s) 186
NciI CCSGG 1 cut(s) 373
NdeII GATC 6 cut(s) 34, 60, 174, 209, 223, 900
NlaIII CATG 1 cut(s) 946
NlaIV GGNNCC 3 cut(s) 666, 741, 862
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 449, 677
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 2 cut(s) 52, 813
PfeI GAWTC 4 cut(s) 218, 499, 536, 786
PkrI GCNGC 5 cut(s) 16, 155, 345, 853, 940
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 3 cut(s) 666, 741, 862
PspPI GGNCC 2 cut(s) 355, 860
PstI CTGCAG 1 cut(s) 856
PsuI RGATCY 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 730
Rsr2I CGGWCCG 1 cut(s) 355
RsrII CGGWCCG 1 cut(s) 355
SaqAI TTAA 3 cut(s) 770, 977, 991
SatI GCNGC 5 cut(s) 15, 154, 344, 852, 939
Sau3AI GATC 6 cut(s) 34, 60, 174, 209, 223, 900
Sau96I GGNCC 2 cut(s) 355, 860
SchI GAGTC 1 cut(s) 159
ScrFI CCNGG 2 cut(s) 373, 669
SfaNI GCATC 4 cut(s) 685, 722, 766, 925
SfcI CTRYAG 1 cut(s) 852
SinI GGWCC 2 cut(s) 355, 860
SmiMI CAYNNNNRTG 1 cut(s) 730
SmlI CTYRAG 2 cut(s) 437, 876
SmoI CTYRAG 2 cut(s) 437, 876
SpeI ACTAGT 1 cut(s) 958
Sse9I AATT 9 cut(s) 20, 319, 325, 338, 471, 527, 606, 865, 905
SsiI CCGC 4 cut(s) 154, 178, 343, 367
SspMI CTAG 2 cut(s) 392, 959
StyD4I CCNGG 2 cut(s) 371, 667
TaaI ACNGT 3 cut(s) 161, 287, 413
TaiI ACGT 1 cut(s) 703
TaqI TCGA 3 cut(s) 44, 173, 573
TaqII GACCGA 1 cut(s) 343
TasI AATT 9 cut(s) 20, 319, 325, 338, 471, 527, 606, 865, 905
TauI GCSGC 2 cut(s) 156, 346
TfiI GAWTC 4 cut(s) 218, 499, 536, 786
Tru1I TTAA 3 cut(s) 770, 977, 991
Tru9I TTAA 3 cut(s) 770, 977, 991
TscAI CASTG 1 cut(s) 521
TseFI GTSAC 2 cut(s) 449, 677
TseI GCWGC 3 cut(s) 14, 851, 938
Tsp45I GTSAC 2 cut(s) 449, 677
TspDTI ATGAA 2 cut(s) 540, 833
TspGWI ACGGA 2 cut(s) 16, 468
TspRI CASTG 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 355, 860
XapI RAATTY 2 cut(s) 325, 527
XmiI GTMKAC 1 cut(s) 824
XspI CTAG 2 cut(s) 392, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.