Rorug02G0123400

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017401
Physical Location & Seq
Reverse (-)
10612899 .. 10620893
7995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0123400.1

Sequence Viewer

Length: 3018 bp
ATGCTAAAGCTCGCGGCGTTTTTCGAGCTTGTGATTGTGGTTGTTTGGTTGGGAGTGGCGAGTGTTGATGGAGTTTTTGTGAAGTACCAGACCGGGAGTGGTGTTGTGGAAGGGAAGTTGAATGTTCATTTGGTTGCGCATTCCCATGATGACGTTGGTTGGTTGAAGACTGTTGATCAGTACTATGTCGGATCAAATAACAGCATTCAGGGTGCTTGTGTTGAGAATACGTTGGATTCTGTCGTTGAATCGTTGGTTCGTGATCCGAATAGGAAGTTTGTGTTTGCTGAGATGGCATTTTTCCAAAGATGGTGGTTAACCCAGAGTGTGGAAGTTCAAGAACGAGTGCGGAAACTAGTAGATGCTGGCCAGTTAGAGTTTGTAAATGGAGGTTGGTGCATGCATGATGAGGCTGCAGTCCATTACATAGACATGATTGACCAAACAACTCTAGGCCACCTTGCTATAAAGCAGCAGTTCAACAAGACACCTAGAGCGGGGTGGCAAATTGATCCTTTCGGACACTCTGCTGTGCAGGGTTACCTACTTGGAGCTGAGCTTGGATTCGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGCACAGCGTAAGGGAGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAAAACTTTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTCGTCACTATAGTCCGCCAGATGGTTTCCATTTTGAAGTTTTTGATGACTTTGTCCCTGTGCAGGATAATCCGCTTATTTTTGACTACAATGTTGAACTGCGCGTTAATGATTTTATTACTGCTGCAATTACACAAGCAAATGTGACTAGGGGAAATCACATGATGTGGACAATGGGAGATGATTTCCAGTACCAATATGCTGAATCTTGGTTCAAACAAATGGACAAGTTAATTCACTATGTTAATAAGGATGGCCGAGTGAATGCCTTATATTCTACACCGTCTCTCTACACTGATGCTAAAAATGCAGCAAATCAATCATGGCCATTAAAAACTGAGGATTATTTTCCGTATGCAGATATAATAAATGCTTATTGGACCGGTTATTTTACTAGTCGCCCAGGTTTGAAAAGATATATTCGATCACTGAGCGGATATTATTTGGCAGCAAGAGAACTAGAATTTCTGGTTGGAAAGAGAGTAAATGGTTCAAGTACCTATTCCCTTGGAGATGCTTTAGGAATTGCACAGCACCACGATGCTGCCACTGGCACTGCTAAACAACATACAACAAATGATTATGTAAAGCGCCTGTCAATTGGTGTCAGTGAGGCAGAAGCTGTTGTCAGTTCTGCTCTGTCGTGCCTAACTACAAATAAATCTGGAGATCAATGTAAAGACCCTGCAGTTACTTTTAGCCAGTGTCGACTACTCAATATCAGTTTCTGCCCTCCAACGGAGGAAGATATTCCAGAAGGGAAGAGCTTGGTTGTTGTGGCATATAATCCTCTTGGATGGAATCGCACTGAGATTGTTAGGATACCTGTTAATTATGATAATCTTGTTGTTCAAGATTCCTTCGGAAACATCCTCGAGACACAATATGTGAGTATGGATAATGTTACAAGCAATCTAAGAACATTTTATACAGAAGCCTACTTGGGACAGTCATCAAAACAAATTCCCAAGTACTGGCTAATCTTTCAAGCCTCTGTGTCACCACTTGGTTGGGACACATACTTCATCTCTAGAGGAGCTACCAAAGGGAAAAGAAGAGTTGGACTTCTCTCTGTGAAGGACAGTCCACAGAATGAGACCATTGAAATTGGACCAGGAGATCTGAAGATGTCCTTTTCTTCTGCTTCTGGACAACTCAAGCGGATGTATAATTCTAAAACAGGAGTTGATGTACCAATACAGCAGAGCTACCTCTGGTATGGCTCAAGCACCGATGGCCAGGCTTCAGGTGCATATATATTCCGGCCTAATGGTGCCCCTCCAACTATTGTGTCAAGATCTGTACCCTTGAAGGTCTTTCGTGGACCATTATTCGATGAGATTCACCAAGAATTCAGTTCATGGATCTATCAGGTCATTAGAGTTTACAGAGACAAAGAGCATGCCGAAGTTGAATATACTATTGGTCCAATTCCTACAGACGACGGTGTTGGTAAAGAGGTCATCACACGAATGACAGCAAACATGGAGACTAACAAGATGTTTTACACCGATTCCAATGGGAGGGATTTCATTAAAAGGGTACGAGATTATAGAGCAGACTGGAACCTTTCAGTTAATCAACCTGTTGCAGGAAACTACTATCCACTAAACCTTGGGATCTTTACTTCGGATAACAAATCTGAGTTCTCAGTCTTGGTTGATCGTGCCACTGGAGGATCCAGCATTCAAGACGGACAAGTAGAGATGATGCTACATAGGCGCCTACTGGTCGATGATGGTAGAGGGGTGGGTGAAGCCCTTGATGAAACAGTGTGCATCAAGAATAATACATGTCAAGGACTAACGGTTCGAGGAAACTATTATATGAGCATAAACCAGTTCGGGGATGGTGCCCAATGGCGTCGGACAACTGGACAAGAAGTTTACTCGCCACTTCTTCTAGCTTTCACCCATGAGAACTTGGAGAATTGGACTGCATCTCATTTAACAAAAGGGTCTGTCATGGATTCAAGTTACAGCTTGCCTCCCAATGTTGCTTTGATTACTCTTCAGGAGTTGGATGGTGGAAGTGTACTTCTCCGTCTAGCACATCTATATGAGGCTGATGAAGCTCCTCAATATTCTACGTTGGCAAAAGTTGAACTGAAGAAGATGCTAACAGGAAAAATGATAAAGGAGTTGAAAGAAGTGAGCTTGTCAGCAAACCAAGAGAAGTCAGAGATGAAGAAGATGACATGGAAAGTCGAGGGTTCAACAGGAGAAGAACCTACACCAGTAAGAGGCGGCCCTGTCGATAGTTCAACTCTTGTTGTTGAGCTTGGACCCATGGAGATACGTACCTTCTTGTTGAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1005

Amino Acids

112.8

Weight (kDa)

5.75

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 41 - 352 1e-77 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 357 - 449 4.6e-18 Alpha mannosidase middle domain
Glyco_hydro_38C PF07748 605 - 814 1.3e-45 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 897 - 1001 4.6e-08 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 138
AccB1I GGYRCC 3 cut(s) 1970, 2418, 2549
AccB7I CCANNNNNTGG 2 cut(s) 328, 1671
AccBSI CCGCTC 2 cut(s) 497, 1131
AccI GTMKAC 1 cut(s) 1405
AccII CGCG 2 cut(s) 14, 801
AciI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2943
AclWI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AcoI YGGCCR 4 cut(s) 367, 952, 1022, 1933
AcsI RAATTY 4 cut(s) 1160, 1659, 2048, 3011
AcuI CTGAAG 4 cut(s) 1841, 1926, 2693, 2825
AcyI GRCGYC 2 cut(s) 2419, 2560
AflIII ACRYGT 1 cut(s) 2489
AgeI ACCGGT 1 cut(s) 1079
AhlI ACTAGT 2 cut(s) 355, 1091
AjnI CCWGG 3 cut(s) 1099, 1810, 1935
AjuI GAANNNNNNNTTGG 6 cut(s) 113, 145, 1152, 1184, 2103, 2135
AloI GAACNNNNNNTCC 2 cut(s) 2490, 2522
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
AlwI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AlwNI CAGNNNCTG 2 cut(s) 1319, 1425
Ama87I CYCGRG 1 cut(s) 1571
AoxI GGCC 7 cut(s) 367, 454, 952, 1022, 1933, 1961, 2944
ApeKI GCWGC 6 cut(s) 413, 472, 821, 1007, 1145, 1241
ApoI RAATTY 4 cut(s) 1160, 1659, 2048, 3011
ArsI GACNNNNNNTTYG 2 cut(s) 2490, 2522
AsiGI ACCGGT 1 cut(s) 1079
Asp700I GAANNNNTTC 1 cut(s) 1446
AspLEI GCGC 4 cut(s) 139, 801, 1290, 2421
AspS9I GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 4 cut(s) 1689, 2033, 2462, 2599
AvaI CYCGRG 1 cut(s) 1571
AvaII GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
BaeGI GKGCMC 2 cut(s) 1975, 2554
BalI TGGCCA 3 cut(s) 369, 1024, 1935
BamHI GGATCC 2 cut(s) 650, 2375
BanI GGYRCC 3 cut(s) 1970, 2418, 2549
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 6 cut(s) 400, 484, 808, 1019, 1157, 1228
BciT130I CCWGG 3 cut(s) 1101, 1812, 1937
BciVI GTATCC 1 cut(s) 1512
BclI TGATCA 1 cut(s) 175
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BcuI ACTAGT 2 cut(s) 355, 1091
BfaI CTAG 9 cut(s) 356, 452, 492, 846, 1092, 1157, 1728, 2600, 2744
BfmI CTRYAG 4 cut(s) 414, 706, 1383, 2133
BfoI RGCGCY 2 cut(s) 1291, 2422
BfuI GTATCC 1 cut(s) 1512
BglII AGATCT 2 cut(s) 1816, 1994
BisI GCNGC 8 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2944
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 8 cut(s) 16, 415, 474, 823, 1009, 1147, 1243, 2945
BmcAI AGTACT 2 cut(s) 182, 1670
Bme1390I CCNGG 4 cut(s) 94, 1101, 1812, 1937
Bme18I GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
BmeT110I CYCGRG 1 cut(s) 1571
BmgT120I GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
BmiI GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2420, 2551, 2983
BmrFI CCNGG 4 cut(s) 94, 1101, 1812, 1937
BmsI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2484, 2645, 2802
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 2 cut(s) 1383, 2391
Bpu1102I GCTNAGC 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 1838, 1906
BpuMI CCSGG 1 cut(s) 94
BsaAI YACGTR 1 cut(s) 2996
BsaHI GRCGYC 2 cut(s) 2419, 2560
BsaI GGTCTC 1 cut(s) 1787
BsaJI CCNNGG 4 cut(s) 1099, 1204, 2311, 2985
BsaWI WCCGGW 1 cut(s) 1079
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1872, 1902
Bse118I RCCGGY 1 cut(s) 1079
BseBI CCWGG 3 cut(s) 1101, 1812, 1937
BseDI CCNNGG 4 cut(s) 1099, 1204, 2311, 2985
BseGI GGATG 6 cut(s) 955, 1499, 1566, 1866, 2551, 2725
BseMII CTCAG 8 cut(s) 279, 546, 689, 1026, 1118, 1497, 2331, 2361
BseRI GAGGAG 2 cut(s) 1746, 2763
BseSI GKGCMC 2 cut(s) 1975, 2554
BseXI GCAGC 6 cut(s) 400, 484, 808, 1019, 1157, 1228
BsgI GTGCAG 2 cut(s) 554, 779
Bsh1236I CGCG 2 cut(s) 14, 801
BshFI GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
BshNI GGYRCC 3 cut(s) 1970, 2418, 2549
BshTI ACCGGT 1 cut(s) 1079
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 1 cut(s) 1571
BsiSI CCGG 3 cut(s) 93, 1080, 1960
BslFI GGGAC 3 cut(s) 737, 1656, 1724
BsmAI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BsmBI CGTCTC 1 cut(s) 987
BsmFI GGGAC 3 cut(s) 737, 1656, 1724
BsmI GAATGC 4 cut(s) 139, 204, 967, 2382
BsnI GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
Bso31I GGTCTC 1 cut(s) 1787
BsoBI CYCGRG 1 cut(s) 1571
Bsp1286I GDGCHC 3 cut(s) 610, 1975, 2554
Bsp1407I TGTACA 1 cut(s) 574
Bsp1720I GCTNAGC 1 cut(s) 555
Bsp19I CCATGG 1 cut(s) 2985
BspACI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2943
BspANI GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
BspCNI CTCAG 8 cut(s) 280, 547, 688, 1027, 1119, 1498, 2332, 2360
BspFNI CGCG 2 cut(s) 14, 801
BspLI GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2420, 2551, 2983
BspMAI CTGCAG 2 cut(s) 418, 1387
BspPI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
BspQI GCTCTTC 1 cut(s) 1454
BspT107I GGYRCC 3 cut(s) 1970, 2418, 2549
BspTNI GGTCTC 1 cut(s) 1787
BsrBI CCGCTC 2 cut(s) 497, 1131
BsrFI RCCGGY 1 cut(s) 1079
BsrGI TGTACA 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 1079
BssECI CCNNGG 4 cut(s) 1099, 1204, 2311, 2985
BssNI GRCGYC 2 cut(s) 2419, 2560
BssT1I CCWWGG 3 cut(s) 1204, 2311, 2985
Bst2UI CCWGG 3 cut(s) 1101, 1812, 1937
Bst4CI ACNGT 7 cut(s) 172, 981, 1647, 1781, 2144, 2470, 2506
Bst6I CTCTTC 3 cut(s) 1454, 1747, 2712
BstACI GRCGYC 2 cut(s) 2419, 2560
BstAUI TGTACA 1 cut(s) 574
BstBAI YACGTR 1 cut(s) 2996
BstC8I GCNNGC 5 cut(s) 12, 367, 401, 2100, 2681
BstDEI CTNAG 9 cut(s) 288, 555, 675, 1035, 1127, 1506, 1613, 2340, 2347
BstDSI CCRYGG 1 cut(s) 2985
BstEII GGTNACC 1 cut(s) 539
BstENI CCTNNNNNAGG 1 cut(s) 2286
BstF5I GGATG 6 cut(s) 955, 1499, 1566, 1866, 2551, 2725
BstFNI CGCG 2 cut(s) 14, 801
BstH2I RGCGCY 2 cut(s) 1291, 2422
BstHHI GCGC 4 cut(s) 139, 801, 1290, 2421
BstMAI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BstMWI GCNNNNNNNGC 6 cut(s) 293, 1004, 1932, 1946, 2416, 2768
BstNI CCWGG 3 cut(s) 1101, 1812, 1937
BstNSI RCATGY 3 cut(s) 403, 2102, 2493
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 4 cut(s) 92, 1099, 1810, 1935
BstSFI CTRYAG 4 cut(s) 414, 706, 1383, 2133
BstSLI GKGCMC 2 cut(s) 1975, 2554
BstSNI TACGTA 1 cut(s) 2996
BstUI CGCG 2 cut(s) 14, 801
BstV1I GCAGC 6 cut(s) 400, 484, 808, 1019, 1157, 1228
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
BstXI CCANNNNNNTGG 1 cut(s) 1707
BstYI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
BsuI GTATCC 1 cut(s) 1512
BsuRI GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
BtgI CCRYGG 1 cut(s) 2985
BtsCI GGATG 6 cut(s) 955, 1499, 1566, 1866, 2551, 2725
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 9 cut(s) 990, 1124, 1245, 1251, 1312, 1406, 1503, 2367, 2475
Cac8I GCNNGC 5 cut(s) 12, 367, 401, 2100, 2681
CaiI CAGNNNCTG 2 cut(s) 1319, 1425
CfoI GCGC 4 cut(s) 139, 801, 1290, 2421
Cfr10I RCCGGY 1 cut(s) 1079
Cfr13I GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
CseI GACGC 1 cut(s) 2549
CspAI ACCGGT 1 cut(s) 1079
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
DdeI CTNAG 9 cut(s) 288, 555, 675, 1035, 1127, 1506, 1613, 2340, 2347
DinI GGCGCC 1 cut(s) 2420
EaeI YGGCCR 4 cut(s) 367, 952, 1022, 1933
Eam1104I CTCTTC 3 cut(s) 1454, 1747, 2712
EarI CTCTTC 3 cut(s) 1454, 1747, 2712
EciI GGCGGA 1 cut(s) 702
Eco105I TACGTA 1 cut(s) 2996
Eco130I CCWWGG 3 cut(s) 1204, 2311, 2985
Eco31I GGTCTC 1 cut(s) 1787
Eco47I GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
Eco57I CTGAAG 4 cut(s) 1841, 1926, 2693, 2825
Eco88I CYCGRG 1 cut(s) 1571
Eco91I GGTNACC 1 cut(s) 539
EcoNI CCTNNNNNAGG 1 cut(s) 2286
EcoO65I GGTNACC 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 2048
EcoRII CCWGG 3 cut(s) 1099, 1810, 1935
EcoT14I CCWWGG 3 cut(s) 1204, 2311, 2985
EcoT22I ATGCAT 1 cut(s) 405
EgeI GGCGCC 1 cut(s) 2420
EheI GGCGCC 1 cut(s) 2420
ErhI CCWWGG 3 cut(s) 1204, 2311, 2985
Esp3I CGTCTC 1 cut(s) 987
FalI AAGNNNNNCTT 2 cut(s) 1814, 1846
FaqI GGGAC 3 cut(s) 737, 1656, 1724
FauI CCCGC 1 cut(s) 490
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 1 cut(s) 1405
Fnu4HI GCNGC 8 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2944
FokI GGATG 6 cut(s) 962, 1506, 1553, 1873, 2558, 2732
Fsp4HI GCNGC 8 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2944
FspBI CTAG 9 cut(s) 356, 452, 492, 846, 1092, 1157, 1728, 2600, 2744
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 4 cut(s) 138, 800, 1289, 2420
GluI GCNGC 8 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2944
GsuI CTGGAG 2 cut(s) 1383, 2391
HaeII RGCGCY 2 cut(s) 1291, 2422
HaeIII GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
HapII CCGG 3 cut(s) 93, 1080, 1960
HgaI GACGC 1 cut(s) 2549
HhaI GCGC 4 cut(s) 139, 801, 1290, 2421
Hin1I GRCGYC 2 cut(s) 2419, 2560
Hin6I GCGC 4 cut(s) 137, 799, 1288, 2419
HinP1I GCGC 4 cut(s) 137, 799, 1288, 2419
HincII GTYRAC 2 cut(s) 318, 1406
HindII GTYRAC 2 cut(s) 318, 1406
HpaI GTTAAC 1 cut(s) 318
HpaII CCGG 3 cut(s) 93, 1080, 1960
HphI GGTGA 4 cut(s) 1689, 2033, 2462, 2599
Hpy166II GTNNAC 8 cut(s) 318, 867, 1406, 1784, 2021, 2083, 2584, 2732
Hpy8I GTNNAC 8 cut(s) 318, 867, 1406, 1784, 2021, 2083, 2584, 2732
Hpy99I CGWCG 2 cut(s) 2144, 2565
HpyAV CCTTC 6 cut(s) 104, 1448, 1567, 1768, 2002, 3010
HpyCH4III ACNGT 7 cut(s) 172, 981, 1647, 1781, 2144, 2470, 2506
HpyCH4IV ACGT 4 cut(s) 153, 230, 2786, 2995
HpyF10VI GCNNNNNNNGC 6 cut(s) 293, 1004, 1932, 1946, 2416, 2768
HpyF3I CTNAG 9 cut(s) 288, 555, 675, 1035, 1127, 1506, 1613, 2340, 2347
HpySE526I ACGT 4 cut(s) 153, 230, 2786, 2995
Hsp92I GRCGYC 2 cut(s) 2419, 2560
HspAI GCGC 4 cut(s) 137, 799, 1288, 2419
KasI GGCGCC 1 cut(s) 2418
Ksp22I TGATCA 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 318
LguI GCTCTTC 1 cut(s) 1454
LmnI GCTCC 3 cut(s) 551, 1733, 2776
Lsp1109I GCAGC 6 cut(s) 400, 484, 808, 1019, 1157, 1228
LweI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2484, 2645, 2802
MaeI CTAG 9 cut(s) 356, 452, 492, 846, 1092, 1157, 1728, 2600, 2744
MaeII ACGT 4 cut(s) 153, 230, 2786, 2995
MaeIII GTNAC 7 cut(s) 539, 701, 841, 1387, 1600, 1695, 2672
MbiI CCGCTC 2 cut(s) 497, 1131
MfeI CAATTG 1 cut(s) 1296
MflI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
MhlI GDGCHC 3 cut(s) 610, 1975, 2554
MlsI TGGCCA 3 cut(s) 369, 1024, 1935
MluNI TGGCCA 3 cut(s) 369, 1024, 1935
Mly113I GGCGCC 1 cut(s) 2419
MmeI TCCRAC 8 cut(s) 169, 213, 1150, 1457, 1738, 2003, 2543, 2697
Mox20I TGGCCA 3 cut(s) 369, 1024, 1935
Mph1103I ATGCAT 1 cut(s) 405
MroXI GAANNNNTTC 1 cut(s) 1446
MscI TGGCCA 3 cut(s) 369, 1024, 1935
MseI TTAA 9 cut(s) 317, 804, 929, 942, 1028, 1527, 2232, 2274, 2645
MslI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2754
Msp20I TGGCCA 3 cut(s) 369, 1024, 1935
MspI CCGG 3 cut(s) 93, 1080, 1960
MspR9I CCNGG 4 cut(s) 94, 1101, 1812, 1937
MunI CAATTG 1 cut(s) 1296
Mva1269I GAATGC 4 cut(s) 139, 204, 967, 2382
MvaI CCWGG 3 cut(s) 1101, 1812, 1937
MvnI CGCG 2 cut(s) 14, 801
MwoI GCNNNNNNNGC 6 cut(s) 293, 1004, 1932, 1946, 2416, 2768
NarI GGCGCC 1 cut(s) 2419
NciI CCSGG 1 cut(s) 94
NcoI CCATGG 1 cut(s) 2985
NlaIV GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2420, 2551, 2983
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 3 cut(s) 701, 841, 1695
NsbI TGCGCA 1 cut(s) 138
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 3 cut(s) 403, 2102, 2493
PaeI GCATGC 2 cut(s) 403, 2102
PaeR7I CTCGAG 1 cut(s) 1571
PciI ACATGT 1 cut(s) 2489
PciSI GCTCTTC 1 cut(s) 1454
PctI GAATGC 4 cut(s) 139, 204, 967, 2382
PdmI GAANNNNTTC 1 cut(s) 1446
PflFI GACNNNGTC 1 cut(s) 749
PflMI CCANNNNNTGG 2 cut(s) 328, 1671
PinAI ACCGGT 1 cut(s) 1079
PkrI GCNGC 8 cut(s) 16, 415, 474, 823, 1009, 1147, 1243, 2945
PluTI GGCGCC 1 cut(s) 2422
Ppu21I YACGTR 1 cut(s) 2996
PscI ACATGT 1 cut(s) 2489
Psp6I CCWGG 3 cut(s) 1099, 1810, 1935
PspEI GGTNACC 1 cut(s) 539
PspGI CCWGG 3 cut(s) 1099, 1810, 1935
PspN4I GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2420, 2551, 2983
PspPI GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
PsrI GAACNNNNNNTAC 2 cut(s) 1609, 1641
PstI CTGCAG 2 cut(s) 418, 1387
PstNI CAGNNNCTG 2 cut(s) 1319, 1425
PsuI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
PsyI GACNNNGTC 1 cut(s) 749
RseI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2754
SalI GTCGAC 1 cut(s) 1404
SapI GCTCTTC 1 cut(s) 1454
SaqAI TTAA 9 cut(s) 317, 804, 929, 942, 1028, 1527, 2232, 2274, 2645
SatI GCNGC 8 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2944
Sau96I GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
ScaI AGTACT 2 cut(s) 182, 1670
ScrFI CCNGG 4 cut(s) 94, 1101, 1812, 1937
SduI GDGCHC 3 cut(s) 610, 1975, 2554
SfaNI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2484, 2645, 2802
SfcI CTRYAG 4 cut(s) 414, 706, 1383, 2133
SfoI GGCGCC 1 cut(s) 2420
Sfr274I CTCGAG 1 cut(s) 1571
SinI GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
SlaI CTCGAG 1 cut(s) 1571
SmiMI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2754
SmlI CTYRAG 3 cut(s) 1571, 1853, 1921
SmoI CTYRAG 3 cut(s) 1571, 1853, 1921
SnaBI TACGTA 1 cut(s) 2996
SpeI ACTAGT 2 cut(s) 355, 1091
SphI GCATGC 2 cut(s) 403, 2102
SsiI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2943
SspDI GGCGCC 1 cut(s) 2418
SspI AATATT 1 cut(s) 2780
SspMI CTAG 9 cut(s) 356, 452, 492, 846, 1092, 1157, 1728, 2600, 2744
StyD4I CCNGG 4 cut(s) 92, 1099, 1810, 1935
StyI CCWWGG 3 cut(s) 1204, 2311, 2985
TaaI ACNGT 7 cut(s) 172, 981, 1647, 1781, 2144, 2470, 2506
TaiI ACGT 4 cut(s) 156, 233, 2789, 2998
TatI WGTACW 4 cut(s) 180, 574, 1668, 2731
TauI GCSGC 2 cut(s) 17, 2946
Tru1I TTAA 9 cut(s) 317, 804, 929, 942, 1028, 1527, 2232, 2274, 2645
Tru9I TTAA 9 cut(s) 317, 804, 929, 942, 1028, 1527, 2232, 2274, 2645
TscAI CASTG 9 cut(s) 997, 1131, 1252, 1258, 1312, 1406, 1510, 2374, 2475
TseFI GTSAC 3 cut(s) 701, 841, 1695
TseI GCWGC 6 cut(s) 413, 472, 821, 1007, 1145, 1241
Tsp45I GTSAC 3 cut(s) 701, 841, 1695
TspDTI ATGAA 8 cut(s) 116, 660, 1711, 2046, 2218, 2478, 2781, 2897
TspGWI ACGGA 4 cut(s) 1038, 1451, 2406, 2729
TspRI CASTG 9 cut(s) 997, 1131, 1252, 1258, 1312, 1406, 1510, 2374, 2475
Tth111I GACNNNGTC 1 cut(s) 749
Van91I CCANNNNNTGG 2 cut(s) 328, 1671
VpaK11BI GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
XagI CCTNNNNNAGG 1 cut(s) 2286
XapI RAATTY 4 cut(s) 1160, 1659, 2048, 3011
XbaI TCTAGA 1 cut(s) 1727
XceI RCATGY 3 cut(s) 403, 2102, 2493
XcmI CCANNNNNNNNNTGG 3 cut(s) 95, 152, 2543
XhoI CTCGAG 1 cut(s) 1571
XmiI GTMKAC 1 cut(s) 1405
XmnI GAANNNNTTC 1 cut(s) 1446
XspI CTAG 9 cut(s) 356, 452, 492, 846, 1092, 1157, 1728, 2600, 2744
ZrmI AGTACT 2 cut(s) 182, 1670
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.