Rh2AG173500

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017401
Physical Location & Seq
Forward (+)
16536116 .. 16539680
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG173500.1

Sequence Viewer

Length: 987 bp
ATGGCCGGAGGTGAGCTTGTTGCAGGGGCAGCTCTGGGATTACCATTTAATATCCTGTATGATGTGATTAAGGAAGTGATGAACAAGACTACAATGTTTAAACCCCTTCTCGGAGATCTCAGCTCCACCATGGAATCGTTACAACCACTGATCACTGACATAGCCAAGCACAACAGGGACTTGGGACGTCCAGAGGAGGAGCTACAAAGTTTCAAGAAGCAAATGGAGAACGGCGCAGAGCTTGTTCGAAAGTGCTCCAAGGTTGGTTTATGGACCAGCTACAAGAAATATGGATACGCCAACAAGTTAATGGACCTAGACAAATCTCTCAACAGATTGTTGGCTATACTCAGCGTTCAGGGAATAAGGGCCGGGATGGAGACCTTGGTGGTGGTGAAGGAAACACTGACTACGGTGAGGAGAATTGAAGATAATGGTGTTGGTAAAGAAGAGAAAGTTGTGGTGCATAATCTGATCGAGTTTCAAAAGGGAAAGCTTATTGGGAGTGGCCCGTTCGGACCTCGCTATGTTGCCAGCAATAGAGTAACTGGAGCTTTATGTTCAATGAAGGAAGTTGACTCATCTCCCAATGGCTCAATTTCCGCAAAATGTTTAGAGACGGAAATTAGAGTGCTCACCAAGCTGAGGCATCCTAACATTGTGGAGTTTTATGGAAGCCAAATGGTTGGTGATCGATTTTATATATATCAGGAGTATGTCCACCCTGGCTCTTTGAGCAGATATATGAATGAACGTTATGGAGCCATAACAGAATCTATTGTTCGTAATTTTACTCGGCAGATAATGTCTGGGTTGGCTTACATGCATAGCAAACACATAGCACACAGGAGCATCAAAGGGGCTAATTTGCTTGTCGATTCAAGTGGAATTGTCAAGCTAGCTGACTTTGGGATGGCACGATTAGTAAGTACTAGATCTTTAATTTCTTCATTCTCAACTCTGATGATCTTCTATTTCTTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

328

Amino Acids

36.57

Weight (kDa)

9.37

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 139 2e-32 Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase PF00069 161 - 315 2.9e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 163 - 311 5.2e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 190
AciI CCGC 1 cut(s) 603
AclI AACGTT 1 cut(s) 754
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 1 cut(s) 931
AfiI CCNNNNNNNGG 2 cut(s) 110, 645
AgsI TTSAA 5 cut(s) 214, 428, 485, 564, 882
AjnI CCWGG 1 cut(s) 724
Alw21I GWGCWC 2 cut(s) 257, 636
Alw26I GTCTC 2 cut(s) 374, 611
AoxI GGCC 3 cut(s) 3, 369, 508
ApeKI GCWGC 1 cut(s) 29
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 5 cut(s) 273, 313, 369, 509, 518
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 5 cut(s) 23, 406, 427, 628, 701
AsuII TTCGAA 1 cut(s) 247
AsuNHI GCTAGC 1 cut(s) 898
AvaII GGWCC 3 cut(s) 273, 313, 518
Bbv12I GWGCWC 2 cut(s) 257, 636
BbvCI CCTCAGC 1 cut(s) 644
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 370, 907
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 726
BciVI GTATCC 1 cut(s) 287
BclI TGATCA 1 cut(s) 150
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 374, 611
BfaI CTAG 3 cut(s) 317, 899, 933
BfuI GTATCC 1 cut(s) 287
BglII AGATCT 2 cut(s) 115, 935
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmcAI AGTACT 1 cut(s) 931
Bme1390I CCNGG 2 cut(s) 373, 726
Bme18I GGWCC 3 cut(s) 273, 313, 518
BmgT120I GGNCC 5 cut(s) 273, 313, 369, 509, 518
BmiI GGNNCC 1 cut(s) 763
BmrFI CCNGG 2 cut(s) 373, 726
BmsI GCATC 2 cut(s) 658, 861
BmtI GCTAGC 1 cut(s) 902
BpmI CTGGAG 1 cut(s) 570
Bpu10I CCTNAGC 1 cut(s) 644
Bpu14I TTCGAA 1 cut(s) 247
BpuMI CCSGG 1 cut(s) 373
Bsa29I ATCGAT 1 cut(s) 694
BsaHI GRCGYC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 4 cut(s) 129, 258, 384, 724
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 645
Bse1I ACTGG 1 cut(s) 553
BseBI CCWGG 1 cut(s) 726
BseCI ATCGAT 1 cut(s) 694
BseDI CCNNGG 4 cut(s) 129, 258, 384, 724
BseGI GGATG 3 cut(s) 381, 649, 918
BseLI CCNNNNNNNGG 2 cut(s) 110, 645
BseMII CTCAG 3 cut(s) 133, 364, 635
BseNI ACTGG 1 cut(s) 553
BseRI GAGGAG 3 cut(s) 209, 212, 433
BseXI GCAGC 1 cut(s) 41
BshFI GGCC 3 cut(s) 5, 371, 510
BshVI ATCGAT 1 cut(s) 694
BsiHKAI GWGCWC 2 cut(s) 257, 636
BsiSI CCGG 2 cut(s) 6, 372
BslFI GGGAC 2 cut(s) 191, 198
BslI CCNNNNNNNGG 2 cut(s) 110, 645
BsmAI GTCTC 2 cut(s) 374, 611
BsmBI CGTCTC 1 cut(s) 611
BsmFI GGGAC 2 cut(s) 191, 198
BsnI GGCC 3 cut(s) 5, 371, 510
Bso31I GGTCTC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 247
Bsp1286I GDGCHC 2 cut(s) 257, 636
Bsp143I GATC 6 cut(s) 115, 150, 474, 691, 935, 966
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 1 cut(s) 603
BspANI GGCC 3 cut(s) 5, 371, 510
BspCNI CTCAG 3 cut(s) 132, 363, 636
BspDI ATCGAT 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 763
BspOI GCTAGC 1 cut(s) 902
BspT104I TTCGAA 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 374
BsrI ACTGG 1 cut(s) 553
BssECI CCNNGG 4 cut(s) 129, 258, 384, 724
BssMI GATC 6 cut(s) 115, 150, 474, 691, 935, 966
BssNI GRCGYC 1 cut(s) 187
BssT1I CCWWGG 3 cut(s) 129, 258, 384
Bst2UI CCWGG 1 cut(s) 726
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 444
BstACI GRCGYC 1 cut(s) 187
BstBI TTCGAA 1 cut(s) 247
BstC8I GCNNGC 2 cut(s) 535, 900
BstDEI CTNAG 3 cut(s) 119, 350, 644
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 3 cut(s) 381, 649, 918
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 6 cut(s) 118, 153, 477, 694, 938, 969
BstMAI GTCTC 2 cut(s) 374, 611
BstMBI GATC 6 cut(s) 115, 150, 474, 691, 935, 966
BstMWI GCNNNNNNNGC 3 cut(s) 29, 640, 735
BstNI CCWGG 1 cut(s) 726
BstNSI RCATGY 1 cut(s) 826
BstSCI CCNGG 2 cut(s) 371, 724
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 2 cut(s) 115, 935
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 2 cut(s) 115, 935
Bsu15I ATCGAT 1 cut(s) 694
BsuI GTATCC 1 cut(s) 287
BsuRI GGCC 3 cut(s) 5, 371, 510
BsuTUI ATCGAT 1 cut(s) 694
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 3 cut(s) 381, 649, 918
BtsIMutI CAGTG 3 cut(s) 146, 153, 404
Cac8I GCNNGC 2 cut(s) 535, 900
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 5 cut(s) 273, 313, 369, 509, 518
ClaI ATCGAT 1 cut(s) 694
Csp6I GTAC 1 cut(s) 930
CviAII CATG 2 cut(s) 130, 823
CviQI GTAC 1 cut(s) 930
DdeI CTNAG 3 cut(s) 119, 350, 644
DpnI GATC 6 cut(s) 117, 152, 476, 693, 937, 968
DpnII GATC 6 cut(s) 115, 150, 474, 691, 935, 966
DraI TTTAAA 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Eco130I CCWWGG 3 cut(s) 129, 258, 384
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 3 cut(s) 273, 313, 518
EcoRII CCWGG 1 cut(s) 724
EcoT14I CCWWGG 3 cut(s) 129, 258, 384
EcoT22I ATGCAT 1 cut(s) 828
ErhI CCWWGG 3 cut(s) 129, 258, 384
Esp3I CGTCTC 1 cut(s) 611
FaeI CATG 2 cut(s) 133, 826
FaqI GGGAC 2 cut(s) 191, 198
FatI CATG 2 cut(s) 129, 822
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 3 cut(s) 388, 636, 925
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 3 cut(s) 317, 899, 933
GlaI GCGC 1 cut(s) 235
GluI GCNGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 3 cut(s) 5, 371, 510
HapII CCGG 2 cut(s) 6, 372
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 2 cut(s) 133, 826
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 4 cut(s) 134, 578, 773, 878
HpaII CCGG 2 cut(s) 6, 372
HphI GGTGA 5 cut(s) 23, 406, 427, 628, 701
Hpy166II GTNNAC 2 cut(s) 577, 721
Hpy188I TCNGA 4 cut(s) 113, 474, 518, 963
Hpy188III TCNNGA 3 cut(s) 191, 214, 710
Hpy8I GTNNAC 2 cut(s) 577, 721
HpyAV CCTTC 3 cut(s) 116, 391, 562
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4IV ACGT 2 cut(s) 187, 754
HpyCH4V TGCA 3 cut(s) 23, 466, 826
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 640, 735
HpyF3I CTNAG 3 cut(s) 119, 350, 644
HpySE526I ACGT 2 cut(s) 187, 754
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 2 cut(s) 133, 826
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 6 cut(s) 115, 150, 474, 691, 935, 966
LmnI GCTCC 6 cut(s) 128, 199, 260, 551, 761, 849
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 658, 861
MaeI CTAG 3 cut(s) 317, 899, 933
MaeII ACGT 2 cut(s) 187, 754
MaeIII GTNAC 2 cut(s) 138, 544
MalI GATC 6 cut(s) 117, 152, 476, 693, 937, 968
MboI GATC 6 cut(s) 115, 150, 474, 691, 935, 966
MboII GAAGA 4 cut(s) 440, 461, 939, 961
MflI RGATCY 2 cut(s) 115, 935
MhlI GDGCHC 2 cut(s) 257, 636
MluCI AATT 7 cut(s) 423, 597, 624, 787, 865, 888, 942
MlyI GAGTC 1 cut(s) 572
MnlI CCTC 5 cut(s) 187, 190, 411, 531, 639
Mph1103I ATGCAT 1 cut(s) 828
MseI TTAA 5 cut(s) 48, 69, 99, 308, 941
MspI CCGG 2 cut(s) 6, 372
MspR9I CCNGG 2 cut(s) 373, 726
MssI GTTTAAAC 1 cut(s) 100
MvaI CCWGG 1 cut(s) 726
MwoI GCNNNNNNNGC 3 cut(s) 29, 640, 735
NciI CCSGG 1 cut(s) 373
NcoI CCATGG 1 cut(s) 129
NdeII GATC 6 cut(s) 115, 150, 474, 691, 935, 966
NheI GCTAGC 1 cut(s) 898
NlaIII CATG 2 cut(s) 133, 826
NlaIV GGNNCC 1 cut(s) 763
NmeAIII GCCGAG 1 cut(s) 775
NsiI ATGCAT 1 cut(s) 828
NspI RCATGY 1 cut(s) 826
NspV TTCGAA 1 cut(s) 247
PfeI GAWTC 3 cut(s) 134, 773, 878
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 572
PmeI GTTTAAAC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 572
Psp1406I AACGTT 1 cut(s) 754
Psp6I CCWGG 1 cut(s) 724
PspGI CCWGG 1 cut(s) 724
PspN4I GGNNCC 1 cut(s) 763
PspPI GGNCC 5 cut(s) 273, 313, 369, 509, 518
PsuI RGATCY 2 cut(s) 115, 935
RsaI GTAC 1 cut(s) 931
RsaNI GTAC 1 cut(s) 930
SaqAI TTAA 5 cut(s) 48, 69, 99, 308, 941
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 6 cut(s) 115, 150, 474, 691, 935, 966
Sau96I GGNCC 5 cut(s) 273, 313, 369, 509, 518
ScaI AGTACT 1 cut(s) 931
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 2 cut(s) 373, 726
SduI GDGCHC 2 cut(s) 257, 636
SfaNI GCATC 2 cut(s) 658, 861
SfuI TTCGAA 1 cut(s) 247
SinI GGWCC 3 cut(s) 273, 313, 518
Sse9I AATT 7 cut(s) 423, 597, 624, 787, 865, 888, 942
SsiI CCGC 1 cut(s) 603
SspMI CTAG 3 cut(s) 317, 899, 933
StyD4I CCNGG 2 cut(s) 371, 724
StyI CCWWGG 3 cut(s) 129, 258, 384
TaaI ACNGT 1 cut(s) 415
TaiI ACGT 2 cut(s) 190, 757
TaqI TCGA 4 cut(s) 247, 477, 694, 876
TasI AATT 7 cut(s) 423, 597, 624, 787, 865, 888, 942
TatI WGTACW 1 cut(s) 929
TfiI GAWTC 3 cut(s) 134, 773, 878
Tru1I TTAA 5 cut(s) 48, 69, 99, 308, 941
Tru9I TTAA 5 cut(s) 48, 69, 99, 308, 941
TscAI CASTG 3 cut(s) 153, 160, 411
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 5 cut(s) 95, 581, 761, 765, 939
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 3 cut(s) 153, 160, 411
VpaK11BI GGWCC 3 cut(s) 273, 313, 518
XceI RCATGY 1 cut(s) 826
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XspI CTAG 3 cut(s) 317, 899, 933
ZraI GACGTC 1 cut(s) 188
ZrmI AGTACT 1 cut(s) 931
Zsp2I ATGCAT 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.