Rh2DG179700

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017401
Physical Location & Seq
Forward (+)
15851657 .. 15854958
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG179700.1

Sequence Viewer

Length: 1272 bp
ATGGCCGGAGGTGAGCTTGTTGCAGGGGCAGCTCTGGGATTACCATTTAATATCCTGTATGATGTGATTAAGGAAGTGATGAACAAGACTACAATGTTTAAACCCCTTCTCGGAGATCTCAGCTCCACCATGGAATCGTTACAACCACTGATCACTGACATAGCCAAGCACAACAGGGACTTGGGACGTCCAGAGGAGGAGCTACAAAGTTTCAAGAAGCAAATGGAGAACGGCGCAGAGCTTGTTCGAAAGTGCTCCAAGGTTGGTTTATGGACCAGCTACAAGAAATATGGATACGCCAACAAGTTAATGGACCTAGACAAATCTCTCAACAGATTGTTGGCTATACTCAGCGTTCAGGGAATAAGGGCCGGGATGGAGACCTTGGTGGTGGTGAAGGAAACACTGACTACGGTGAGGAGAATTGAAGATAATGGTGTTGGTAAAGAAGAGAAAGTTGTGGTGCATAATCTGATCGAGTTTCAAAAGGGAAAGCTTATTGGGAGTGGCCCGTTCGGACCTCGCTATGTTGCCAGCAATAGAGTAACTGGAGCTTTATGTTCAATGAAGGAAGTTGACTCATCTCCCAATGGCTCAATTTCCGCAAAATGTTTAGAGACGGAAATTAGAGTGCTCACCAAGCTGAGGCATCCTAACATTGTGGAGTTTTATGGAAGCCAAATGGTTGGTGATCGATTTTATATATATCAGGAGTATGTCCACCCTGGCTCTTTGAGCAGATATATGAATGAACGTTATGGAGCCATAACAGAATCTATTGTTCGTAATTTTACTCGGCAGATAATGTCTGGGTTGGCTTACATGCATAGCAAACACATAGCACACAGGAGCATCAAAGGGGCTAATTTGCTTGTCGATTCAAGTGGAATTGTCAAGCTAGCTGACTTTGGGATGGCACGATTACTTTCAGGGCATGAAGGAAATACATCTCTAGAGGGGGGAAATCCATACTACTGGATGGCTCCAGAGTTAATTATTTCTGGGAGGCAGAGTCTTGCTGCTGATATTTGGAGTTTGGGTTGCACAGTTATTGAAATGCTGACCGGAAGAGCTCATCAGAACGCATATGAAAGTATGGAAGCCTTGTTTAAAACTTCTAGTGGGAATGAGGATAATCCAGAGCTTCCGGGAACATTGTCATTGGAGGGCAAGAATTTCGTAGAATGCTGCTTGCGAATAAATCCTGCAGATAGGCCTACTGCTGCCATGTTGTTAGAACATCCATTCTTAACAAGTTCATCCAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

423

Amino Acids

46.74

Weight (kDa)

8.1

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 139 3.5e-32 Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase PF00069 161 - 417 8.4e-58 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 163 - 413 6.9e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 190
AciI CCGC 1 cut(s) 603
AclI AACGTT 1 cut(s) 754
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 1174
AcyI GRCGYC 1 cut(s) 187
AfiI CCNNNNNNNGG 2 cut(s) 110, 645
AgsI TTSAA 6 cut(s) 214, 428, 485, 564, 882, 1055
AjnI CCWGG 2 cut(s) 724, 1262
Alw21I GWGCWC 3 cut(s) 257, 636, 1075
Alw26I GTCTC 2 cut(s) 374, 611
AoxI GGCC 4 cut(s) 3, 369, 508, 1214
ApeKI GCWGC 4 cut(s) 29, 1019, 1188, 1223
ApoI RAATTY 1 cut(s) 1174
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 5 cut(s) 273, 313, 369, 509, 518
AsuC2I CCSGG 2 cut(s) 373, 1149
AsuHPI GGTGA 5 cut(s) 23, 406, 427, 628, 701
AsuII TTCGAA 1 cut(s) 247
AsuNHI GCTAGC 1 cut(s) 898
AvaII GGWCC 3 cut(s) 273, 313, 518
BanII GRGCYC 1 cut(s) 1075
Bbv12I GWGCWC 3 cut(s) 257, 636, 1075
BbvCI CCTCAGC 1 cut(s) 644
BbvI GCAGC 4 cut(s) 41, 1006, 1175, 1210
BccI CCATC 3 cut(s) 370, 907, 973
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 2 cut(s) 1159, 1193
BciT130I CCWGG 2 cut(s) 726, 1264
BciVI GTATCC 1 cut(s) 287
BclI TGATCA 1 cut(s) 150
BcnI CCSGG 2 cut(s) 373, 1149
BcoDI GTCTC 2 cut(s) 374, 611
BfaI CTAG 4 cut(s) 317, 899, 953, 1119
BfmI CTRYAG 1 cut(s) 1206
BfuI GTATCC 1 cut(s) 287
BglII AGATCT 1 cut(s) 115
BisI GCNGC 4 cut(s) 30, 1020, 1189, 1224
BlsI GCNGC 4 cut(s) 31, 1021, 1190, 1225
Bme1390I CCNGG 4 cut(s) 373, 726, 1149, 1264
Bme18I GGWCC 3 cut(s) 273, 313, 518
BmgT120I GGNCC 5 cut(s) 273, 313, 369, 509, 518
BmiI GGNNCC 2 cut(s) 763, 984
BmrFI CCNGG 4 cut(s) 373, 726, 1149, 1264
BmsI GCATC 2 cut(s) 658, 861
BmtI GCTAGC 1 cut(s) 902
BpmI CTGGAG 2 cut(s) 570, 969
Bpu10I CCTNAGC 1 cut(s) 644
Bpu14I TTCGAA 1 cut(s) 247
BpuMI CCSGG 2 cut(s) 373, 1149
Bsa29I ATCGAT 1 cut(s) 694
BsaHI GRCGYC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 5 cut(s) 129, 258, 384, 724, 1263
BsaWI WCCGGW 1 cut(s) 1064
BsaXI ACNNNNNCTCC 4 cut(s) 997, 1024, 1027, 1054
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 645
Bse1I ACTGG 2 cut(s) 553, 980
BseBI CCWGG 2 cut(s) 726, 1264
BseCI ATCGAT 1 cut(s) 694
BseDI CCNNGG 5 cut(s) 129, 258, 384, 724, 1263
BseGI GGATG 6 cut(s) 381, 649, 918, 984, 1240, 1259
BseLI CCNNNNNNNGG 2 cut(s) 110, 645
BseMII CTCAG 3 cut(s) 133, 364, 635
BseNI ACTGG 2 cut(s) 553, 980
BseRI GAGGAG 3 cut(s) 209, 212, 433
BseXI GCAGC 4 cut(s) 41, 1006, 1175, 1210
BshFI GGCC 4 cut(s) 5, 371, 510, 1216
BshVI ATCGAT 1 cut(s) 694
BsiHKAI GWGCWC 3 cut(s) 257, 636, 1075
BsiSI CCGG 4 cut(s) 6, 372, 1065, 1148
BslFI GGGAC 2 cut(s) 191, 198
BslI CCNNNNNNNGG 2 cut(s) 110, 645
BsmAI GTCTC 2 cut(s) 374, 611
BsmBI CGTCTC 1 cut(s) 611
BsmFI GGGAC 2 cut(s) 191, 198
BsmI GAATGC 1 cut(s) 1190
BsnI GGCC 4 cut(s) 5, 371, 510, 1216
Bso31I GGTCTC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 247
Bsp1286I GDGCHC 3 cut(s) 257, 636, 1075
Bsp143I GATC 4 cut(s) 115, 150, 474, 691
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 1 cut(s) 603
BspANI GGCC 4 cut(s) 5, 371, 510, 1216
BspCNI CTCAG 3 cut(s) 132, 363, 636
BspDI ATCGAT 1 cut(s) 694
BspLI GGNNCC 2 cut(s) 763, 984
BspMAI CTGCAG 1 cut(s) 1210
BspOI GCTAGC 1 cut(s) 902
BspQI GCTCTTC 1 cut(s) 1063
BspT104I TTCGAA 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 374
BsrI ACTGG 2 cut(s) 553, 980
BssECI CCNNGG 5 cut(s) 129, 258, 384, 724, 1263
BssMI GATC 4 cut(s) 115, 150, 474, 691
BssNI GRCGYC 1 cut(s) 187
BssT1I CCWWGG 3 cut(s) 129, 258, 384
Bst2UI CCWGG 2 cut(s) 726, 1264
Bst4CI ACNGT 2 cut(s) 415, 1048
Bst6I CTCTTC 2 cut(s) 444, 1063
BstACI GRCGYC 1 cut(s) 187
BstBI TTCGAA 1 cut(s) 247
BstC8I GCNNGC 3 cut(s) 535, 900, 1193
BstDEI CTNAG 3 cut(s) 119, 350, 644
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 6 cut(s) 381, 649, 918, 984, 1240, 1259
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 4 cut(s) 118, 153, 477, 694
BstMAI GTCTC 2 cut(s) 374, 611
BstMBI GATC 4 cut(s) 115, 150, 474, 691
BstMWI GCNNNNNNNGC 3 cut(s) 29, 640, 735
BstNI CCWGG 2 cut(s) 726, 1264
BstNSI RCATGY 1 cut(s) 826
BstSCI CCNGG 4 cut(s) 371, 724, 1147, 1262
BstSFI CTRYAG 1 cut(s) 1206
BstV1I GCAGC 4 cut(s) 41, 1006, 1175, 1210
BstX2I RGATCY 1 cut(s) 115
BstXI CCANNNNNNTGG 2 cut(s) 686, 975
BstYI RGATCY 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 694
BsuI GTATCC 1 cut(s) 287
BsuRI GGCC 4 cut(s) 5, 371, 510, 1216
BsuTUI ATCGAT 1 cut(s) 694
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 6 cut(s) 381, 649, 918, 984, 1240, 1259
BtsIMutI CAGTG 3 cut(s) 146, 153, 404
Cac8I GCNNGC 3 cut(s) 535, 900, 1193
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 5 cut(s) 273, 313, 369, 509, 518
ClaI ATCGAT 1 cut(s) 694
CviAII CATG 4 cut(s) 130, 823, 935, 1228
DdeI CTNAG 3 cut(s) 119, 350, 644
DpnI GATC 4 cut(s) 117, 152, 476, 693
DpnII GATC 4 cut(s) 115, 150, 474, 691
DraI TTTAAA 2 cut(s) 100, 1111
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 444, 1063
EarI CTCTTC 2 cut(s) 444, 1063
Ecl136II GAGCTC 1 cut(s) 1073
Eco130I CCWWGG 3 cut(s) 129, 258, 384
Eco147I AGGCCT 1 cut(s) 1216
Eco24I GRGCYC 1 cut(s) 1075
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 3 cut(s) 273, 313, 518
Eco53kI GAGCTC 1 cut(s) 1073
EcoICRI GAGCTC 1 cut(s) 1073
EcoRII CCWGG 2 cut(s) 724, 1262
EcoT14I CCWWGG 3 cut(s) 129, 258, 384
EcoT22I ATGCAT 1 cut(s) 828
EcoT38I GRGCYC 1 cut(s) 1075
ErhI CCWWGG 3 cut(s) 129, 258, 384
Esp3I CGTCTC 1 cut(s) 611
FaeI CATG 4 cut(s) 133, 826, 938, 1231
FaqI GGGAC 2 cut(s) 191, 198
FatI CATG 4 cut(s) 129, 822, 934, 1227
FauNDI CATATG 1 cut(s) 1087
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 4 cut(s) 30, 1020, 1189, 1224
FokI GGATG 6 cut(s) 388, 636, 925, 991, 1227, 1246
FriOI GRGCYC 1 cut(s) 1075
Fsp4HI GCNGC 4 cut(s) 30, 1020, 1189, 1224
FspBI CTAG 4 cut(s) 317, 899, 953, 1119
GlaI GCGC 1 cut(s) 235
GluI GCNGC 4 cut(s) 30, 1020, 1189, 1224
GsuI CTGGAG 2 cut(s) 570, 969
HaeIII GGCC 4 cut(s) 5, 371, 510, 1216
HapII CCGG 4 cut(s) 6, 372, 1065, 1148
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 4 cut(s) 133, 826, 938, 1231
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 5 cut(s) 134, 578, 773, 878, 1012
HpaII CCGG 4 cut(s) 6, 372, 1065, 1148
HphI GGTGA 5 cut(s) 23, 406, 427, 628, 701
Hpy166II GTNNAC 2 cut(s) 577, 721
Hpy188I TCNGA 4 cut(s) 113, 474, 518, 1080
Hpy188III TCNNGA 6 cut(s) 191, 214, 710, 953, 986, 1139
Hpy8I GTNNAC 2 cut(s) 577, 721
HpyAV CCTTC 4 cut(s) 116, 391, 562, 932
HpyCH4III ACNGT 2 cut(s) 415, 1048
HpyCH4IV ACGT 2 cut(s) 187, 754
HpyCH4V TGCA 5 cut(s) 23, 466, 826, 1044, 1208
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 640, 735
HpyF3I CTNAG 3 cut(s) 119, 350, 644
HpySE526I ACGT 2 cut(s) 187, 754
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 4 cut(s) 133, 826, 938, 1231
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 4 cut(s) 115, 150, 474, 691
LguI GCTCTTC 1 cut(s) 1063
LmnI GCTCC 7 cut(s) 128, 199, 260, 551, 761, 849, 988
Lsp1109I GCAGC 4 cut(s) 41, 1006, 1175, 1210
LweI GCATC 2 cut(s) 658, 861
MaeI CTAG 4 cut(s) 317, 899, 953, 1119
MaeII ACGT 2 cut(s) 187, 754
MaeIII GTNAC 2 cut(s) 138, 544
MalI GATC 4 cut(s) 117, 152, 476, 693
MboI GATC 4 cut(s) 115, 150, 474, 691
MboII GAAGA 3 cut(s) 440, 461, 1080
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 3 cut(s) 257, 636, 1075
MluCI AATT 8 cut(s) 423, 597, 624, 787, 865, 888, 993, 1174
MlyI GAGTC 2 cut(s) 572, 1021
MnlI CCTC 9 cut(s) 187, 190, 411, 531, 639, 949, 999, 1123, 1159
Mph1103I ATGCAT 1 cut(s) 828
MseI TTAA 7 cut(s) 48, 69, 99, 308, 992, 1110, 1250
MspI CCGG 4 cut(s) 6, 372, 1065, 1148
MspR9I CCNGG 4 cut(s) 373, 726, 1149, 1264
MssI GTTTAAAC 1 cut(s) 100
Mva1269I GAATGC 1 cut(s) 1190
MvaI CCWGG 2 cut(s) 726, 1264
MwoI GCNNNNNNNGC 3 cut(s) 29, 640, 735
NciI CCSGG 2 cut(s) 373, 1149
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 1087
NdeII GATC 4 cut(s) 115, 150, 474, 691
NheI GCTAGC 1 cut(s) 898
NlaIII CATG 4 cut(s) 133, 826, 938, 1231
NlaIV GGNNCC 2 cut(s) 763, 984
NmeAIII GCCGAG 1 cut(s) 775
NsiI ATGCAT 1 cut(s) 828
NspI RCATGY 1 cut(s) 826
NspV TTCGAA 1 cut(s) 247
PceI AGGCCT 1 cut(s) 1216
PciSI GCTCTTC 1 cut(s) 1063
PctI GAATGC 1 cut(s) 1190
PfeI GAWTC 3 cut(s) 134, 773, 878
PfoI TCCNGGA 1 cut(s) 1147
PkrI GCNGC 4 cut(s) 31, 1021, 1190, 1225
PleI GAGTC 2 cut(s) 572, 1020
PmeI GTTTAAAC 1 cut(s) 100
PpsI GAGTC 2 cut(s) 572, 1020
Psp124BI GAGCTC 1 cut(s) 1075
Psp1406I AACGTT 1 cut(s) 754
Psp6I CCWGG 2 cut(s) 724, 1262
PspGI CCWGG 2 cut(s) 724, 1262
PspN4I GGNNCC 2 cut(s) 763, 984
PspPI GGNCC 5 cut(s) 273, 313, 369, 509, 518
PstI CTGCAG 1 cut(s) 1210
PsuI RGATCY 1 cut(s) 115
SacI GAGCTC 1 cut(s) 1075
SapI GCTCTTC 1 cut(s) 1063
SaqAI TTAA 7 cut(s) 48, 69, 99, 308, 992, 1110, 1250
SatI GCNGC 4 cut(s) 30, 1020, 1189, 1224
Sau3AI GATC 4 cut(s) 115, 150, 474, 691
Sau96I GGNCC 5 cut(s) 273, 313, 369, 509, 518
SchI GAGTC 2 cut(s) 572, 1021
ScrFI CCNGG 4 cut(s) 373, 726, 1149, 1264
SduI GDGCHC 3 cut(s) 257, 636, 1075
SfaNI GCATC 2 cut(s) 658, 861
SfcI CTRYAG 1 cut(s) 1206
SfuI TTCGAA 1 cut(s) 247
SinI GGWCC 3 cut(s) 273, 313, 518
Sse9I AATT 8 cut(s) 423, 597, 624, 787, 865, 888, 993, 1174
SseBI AGGCCT 1 cut(s) 1216
SsiI CCGC 1 cut(s) 603
SspMI CTAG 4 cut(s) 317, 899, 953, 1119
SstI GAGCTC 1 cut(s) 1075
StuI AGGCCT 1 cut(s) 1216
StyD4I CCNGG 4 cut(s) 371, 724, 1147, 1262
StyI CCWWGG 3 cut(s) 129, 258, 384
TaaI ACNGT 2 cut(s) 415, 1048
TaiI ACGT 2 cut(s) 190, 757
TaqI TCGA 4 cut(s) 247, 477, 694, 876
TasI AATT 8 cut(s) 423, 597, 624, 787, 865, 888, 993, 1174
TfiI GAWTC 3 cut(s) 134, 773, 878
Tru1I TTAA 7 cut(s) 48, 69, 99, 308, 992, 1110, 1250
Tru9I TTAA 7 cut(s) 48, 69, 99, 308, 992, 1110, 1250
TscAI CASTG 3 cut(s) 153, 160, 411
TseI GCWGC 4 cut(s) 29, 1019, 1188, 1223
TspDTI ATGAA 7 cut(s) 95, 581, 761, 765, 951, 1104, 1248
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 3 cut(s) 153, 160, 411
VpaK11BI GGWCC 3 cut(s) 273, 313, 518
XapI RAATTY 1 cut(s) 1174
XbaI TCTAGA 1 cut(s) 952
XceI RCATGY 1 cut(s) 826
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XspI CTAG 4 cut(s) 317, 899, 953, 1119
ZraI GACGTC 1 cut(s) 188
Zsp2I ATGCAT 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.