Rh2BG181100

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017401
Physical Location & Seq
Forward (+)
16137192 .. 16139518
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG181100.1

Sequence Viewer

Length: 1185 bp
ATGGCCGGAGGTGAGCTTGTTGCAGGGGCAGCTCTGGGATTACCATTTAATATCCTGTATGATGTGATTAAGGAAGTGATGAACAAGACTACAATGTTTAAACCCCTTCTCGGAGATCTCAGCTCCACCATGGAATCGTTACAACCACTGATCACTGACATAGCCAAGCACAACAGGGACTTGGGACGTCCAGAGGAGGAGCTACAAAGTTTCAAGAAGCAAATGGAGAACGGCGCAGAGCTTGTTCGAAAGTGCTCCAAGGTTGGTTTATGGACCAGCTACAAGAAATATGGATACGCCAACAAGTTAATGGACCTAGACAAATCTCTCAACAGATTGTTGGCTATACTCAGCGTTCAGGGAATAAGGGCCGGGATGGAGACCTTGGTGGTGGTGAAGGAAACACTGACTACGGTGAGGAGAATTGAAGATAATGGTGTTGGTAAAGAAGAGAAAGTTGTGGTGCATAATCTGATCGAGTTTCAAAAGGGAAAGCTTATTGGGAGTGGCCCGTTCGGACCTCGCTATGTTGCCAGCAATAGAGTGCTCACCAAGCTGAGGCATCCTAACATTGTGGAGTTTTATGGAAGCCAAATGGTTGGTGATCGATTTTATATATATCAGGAGTATGTCCACCCTGGCTCTTTGAGCAGATATATGAATGAACGTTATGGAGCCATAACAGAATCTATTGTTCGTAATTTTACTCGGCAGATAATGTCTGGGTTGGCTTACATGCATAGCAAACACATAGCACACAGGAGCATCAAAGGGGCTAATTTGCTTGTCGATTCAAGTGGAATTGTCAAGCTAGCTGACTTTGGGATGGCACGATTACTTTCAGGGCATGAAGGAAATACATCTCTAGAGGGGGGAAATCCATACTACTGGATGGCTCCAGAGTTAATTATTTCTGGGAGGCAGAGTCTTGCTGCTGATATTTGGAGTTTGGGTTGCACAGTTATTGAAATGCTGACCGGAAGAGCTCATCAGAACGCATATGAAAGTATGGAAGCCTTGTTTAAAACTTCTAGTGGGAATGAGGATAATCCAGAGCTTCCGGGAACATTGTCATTGGAGGGCAAGAATTTCGTAGAATGCTGCTTGCGAATAAATCCTGCAGATAGGCCTACTGCTGCCATGTTGTTAGAACATCCATTCTTAACAAGTTCATCCAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

394

Amino Acids

43.73

Weight (kDa)

8.48

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 139 3e-32 Arabidopsis broad-spectrum mildew resistance protein RPW8
PK_Tyr_Ser-Thr PF07714 180 - 384 3.9e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 181 - 388 2.7e-49 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 190
AclI AACGTT 1 cut(s) 667
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 1087
AcyI GRCGYC 1 cut(s) 187
AfiI CCNNNNNNNGG 2 cut(s) 110, 558
AgsI TTSAA 5 cut(s) 214, 428, 485, 795, 968
AjnI CCWGG 2 cut(s) 637, 1175
Alw21I GWGCWC 3 cut(s) 257, 549, 988
Alw26I GTCTC 1 cut(s) 374
AoxI GGCC 4 cut(s) 3, 369, 508, 1127
ApeKI GCWGC 4 cut(s) 29, 932, 1101, 1136
ApoI RAATTY 1 cut(s) 1087
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 5 cut(s) 273, 313, 369, 509, 518
AsuC2I CCSGG 2 cut(s) 373, 1062
AsuHPI GGTGA 5 cut(s) 23, 406, 427, 541, 614
AsuII TTCGAA 1 cut(s) 247
AsuNHI GCTAGC 1 cut(s) 811
AvaII GGWCC 3 cut(s) 273, 313, 518
BanII GRGCYC 1 cut(s) 988
Bbv12I GWGCWC 3 cut(s) 257, 549, 988
BbvCI CCTCAGC 1 cut(s) 557
BbvI GCAGC 4 cut(s) 41, 919, 1088, 1123
BccI CCATC 3 cut(s) 370, 820, 886
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 2 cut(s) 1072, 1106
BciT130I CCWGG 2 cut(s) 639, 1177
BciVI GTATCC 1 cut(s) 287
BclI TGATCA 1 cut(s) 150
BcnI CCSGG 2 cut(s) 373, 1062
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 4 cut(s) 317, 812, 866, 1032
BfmI CTRYAG 1 cut(s) 1119
BfuI GTATCC 1 cut(s) 287
BglII AGATCT 1 cut(s) 115
BisI GCNGC 4 cut(s) 30, 933, 1102, 1137
BlsI GCNGC 4 cut(s) 31, 934, 1103, 1138
Bme1390I CCNGG 4 cut(s) 373, 639, 1062, 1177
Bme18I GGWCC 3 cut(s) 273, 313, 518
BmgT120I GGNCC 5 cut(s) 273, 313, 369, 509, 518
BmiI GGNNCC 2 cut(s) 676, 897
BmrFI CCNGG 4 cut(s) 373, 639, 1062, 1177
BmsI GCATC 2 cut(s) 571, 774
BmtI GCTAGC 1 cut(s) 815
BpmI CTGGAG 1 cut(s) 882
Bpu10I CCTNAGC 1 cut(s) 557
Bpu14I TTCGAA 1 cut(s) 247
BpuMI CCSGG 2 cut(s) 373, 1062
Bsa29I ATCGAT 1 cut(s) 607
BsaHI GRCGYC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 5 cut(s) 129, 258, 384, 637, 1176
BsaWI WCCGGW 1 cut(s) 977
BsaXI ACNNNNNCTCC 4 cut(s) 910, 937, 940, 967
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 558
Bse1I ACTGG 1 cut(s) 893
BseBI CCWGG 2 cut(s) 639, 1177
BseCI ATCGAT 1 cut(s) 607
BseDI CCNNGG 5 cut(s) 129, 258, 384, 637, 1176
BseGI GGATG 6 cut(s) 381, 562, 831, 897, 1153, 1172
BseLI CCNNNNNNNGG 2 cut(s) 110, 558
BseMII CTCAG 3 cut(s) 133, 364, 548
BseNI ACTGG 1 cut(s) 893
BseRI GAGGAG 3 cut(s) 209, 212, 433
BseXI GCAGC 4 cut(s) 41, 919, 1088, 1123
BshFI GGCC 4 cut(s) 5, 371, 510, 1129
BshVI ATCGAT 1 cut(s) 607
BsiHKAI GWGCWC 3 cut(s) 257, 549, 988
BsiSI CCGG 4 cut(s) 6, 372, 978, 1061
BslFI GGGAC 2 cut(s) 191, 198
BslI CCNNNNNNNGG 2 cut(s) 110, 558
BsmAI GTCTC 1 cut(s) 374
BsmFI GGGAC 2 cut(s) 191, 198
BsmI GAATGC 1 cut(s) 1103
BsnI GGCC 4 cut(s) 5, 371, 510, 1129
Bso31I GGTCTC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 247
Bsp1286I GDGCHC 3 cut(s) 257, 549, 988
Bsp143I GATC 4 cut(s) 115, 150, 474, 604
Bsp19I CCATGG 1 cut(s) 129
BspANI GGCC 4 cut(s) 5, 371, 510, 1129
BspCNI CTCAG 3 cut(s) 132, 363, 549
BspDI ATCGAT 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 676, 897
BspMAI CTGCAG 1 cut(s) 1123
BspOI GCTAGC 1 cut(s) 815
BspQI GCTCTTC 1 cut(s) 976
BspT104I TTCGAA 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 374
BsrI ACTGG 1 cut(s) 893
BssECI CCNNGG 5 cut(s) 129, 258, 384, 637, 1176
BssMI GATC 4 cut(s) 115, 150, 474, 604
BssNI GRCGYC 1 cut(s) 187
BssT1I CCWWGG 3 cut(s) 129, 258, 384
Bst2UI CCWGG 2 cut(s) 639, 1177
Bst4CI ACNGT 2 cut(s) 415, 961
Bst6I CTCTTC 2 cut(s) 444, 976
BstACI GRCGYC 1 cut(s) 187
BstBI TTCGAA 1 cut(s) 247
BstC8I GCNNGC 3 cut(s) 535, 813, 1106
BstDEI CTNAG 3 cut(s) 119, 350, 557
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 6 cut(s) 381, 562, 831, 897, 1153, 1172
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 4 cut(s) 118, 153, 477, 607
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 4 cut(s) 115, 150, 474, 604
BstMWI GCNNNNNNNGC 3 cut(s) 29, 553, 648
BstNI CCWGG 2 cut(s) 639, 1177
BstNSI RCATGY 1 cut(s) 739
BstSCI CCNGG 4 cut(s) 371, 637, 1060, 1175
BstSFI CTRYAG 1 cut(s) 1119
BstV1I GCAGC 4 cut(s) 41, 919, 1088, 1123
BstX2I RGATCY 1 cut(s) 115
BstXI CCANNNNNNTGG 2 cut(s) 599, 888
BstYI RGATCY 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 607
BsuI GTATCC 1 cut(s) 287
BsuRI GGCC 4 cut(s) 5, 371, 510, 1129
BsuTUI ATCGAT 1 cut(s) 607
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 6 cut(s) 381, 562, 831, 897, 1153, 1172
BtsIMutI CAGTG 3 cut(s) 146, 153, 404
Cac8I GCNNGC 3 cut(s) 535, 813, 1106
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 5 cut(s) 273, 313, 369, 509, 518
ClaI ATCGAT 1 cut(s) 607
CviAII CATG 4 cut(s) 130, 736, 848, 1141
DdeI CTNAG 3 cut(s) 119, 350, 557
DpnI GATC 4 cut(s) 117, 152, 476, 606
DpnII GATC 4 cut(s) 115, 150, 474, 604
DraI TTTAAA 2 cut(s) 100, 1024
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 444, 976
EarI CTCTTC 2 cut(s) 444, 976
Ecl136II GAGCTC 1 cut(s) 986
Eco130I CCWWGG 3 cut(s) 129, 258, 384
Eco147I AGGCCT 1 cut(s) 1129
Eco24I GRGCYC 1 cut(s) 988
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 3 cut(s) 273, 313, 518
Eco53kI GAGCTC 1 cut(s) 986
EcoICRI GAGCTC 1 cut(s) 986
EcoRII CCWGG 2 cut(s) 637, 1175
EcoT14I CCWWGG 3 cut(s) 129, 258, 384
EcoT22I ATGCAT 1 cut(s) 741
EcoT38I GRGCYC 1 cut(s) 988
ErhI CCWWGG 3 cut(s) 129, 258, 384
FaeI CATG 4 cut(s) 133, 739, 851, 1144
FaqI GGGAC 2 cut(s) 191, 198
FatI CATG 4 cut(s) 129, 735, 847, 1140
FauNDI CATATG 1 cut(s) 1000
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 4 cut(s) 30, 933, 1102, 1137
FokI GGATG 6 cut(s) 388, 549, 838, 904, 1140, 1159
FriOI GRGCYC 1 cut(s) 988
Fsp4HI GCNGC 4 cut(s) 30, 933, 1102, 1137
FspBI CTAG 4 cut(s) 317, 812, 866, 1032
GlaI GCGC 1 cut(s) 235
GluI GCNGC 4 cut(s) 30, 933, 1102, 1137
GsuI CTGGAG 1 cut(s) 882
HaeIII GGCC 4 cut(s) 5, 371, 510, 1129
HapII CCGG 4 cut(s) 6, 372, 978, 1061
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 4 cut(s) 133, 739, 851, 1144
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 4 cut(s) 134, 686, 791, 925
HpaII CCGG 4 cut(s) 6, 372, 978, 1061
HphI GGTGA 5 cut(s) 23, 406, 427, 541, 614
Hpy166II GTNNAC 1 cut(s) 634
Hpy188I TCNGA 4 cut(s) 113, 474, 518, 993
Hpy188III TCNNGA 6 cut(s) 191, 214, 623, 866, 899, 1052
Hpy8I GTNNAC 1 cut(s) 634
HpyAV CCTTC 3 cut(s) 116, 391, 845
HpyCH4III ACNGT 2 cut(s) 415, 961
HpyCH4IV ACGT 2 cut(s) 187, 667
HpyCH4V TGCA 5 cut(s) 23, 466, 739, 957, 1121
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 553, 648
HpyF3I CTNAG 3 cut(s) 119, 350, 557
HpySE526I ACGT 2 cut(s) 187, 667
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 4 cut(s) 133, 739, 851, 1144
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 4 cut(s) 115, 150, 474, 604
LguI GCTCTTC 1 cut(s) 976
LmnI GCTCC 6 cut(s) 128, 199, 260, 674, 762, 901
Lsp1109I GCAGC 4 cut(s) 41, 919, 1088, 1123
LweI GCATC 2 cut(s) 571, 774
MaeI CTAG 4 cut(s) 317, 812, 866, 1032
MaeII ACGT 2 cut(s) 187, 667
MaeIII GTNAC 1 cut(s) 138
MalI GATC 4 cut(s) 117, 152, 476, 606
MboI GATC 4 cut(s) 115, 150, 474, 604
MboII GAAGA 3 cut(s) 440, 461, 993
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 3 cut(s) 257, 549, 988
MluCI AATT 6 cut(s) 423, 700, 778, 801, 906, 1087
MlyI GAGTC 1 cut(s) 934
MnlI CCTC 9 cut(s) 187, 190, 411, 531, 552, 862, 912, 1036, 1072
Mph1103I ATGCAT 1 cut(s) 741
MseI TTAA 7 cut(s) 48, 69, 99, 308, 905, 1023, 1163
MspI CCGG 4 cut(s) 6, 372, 978, 1061
MspR9I CCNGG 4 cut(s) 373, 639, 1062, 1177
MssI GTTTAAAC 1 cut(s) 100
Mva1269I GAATGC 1 cut(s) 1103
MvaI CCWGG 2 cut(s) 639, 1177
MwoI GCNNNNNNNGC 3 cut(s) 29, 553, 648
NciI CCSGG 2 cut(s) 373, 1062
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 1000
NdeII GATC 4 cut(s) 115, 150, 474, 604
NheI GCTAGC 1 cut(s) 811
NlaIII CATG 4 cut(s) 133, 739, 851, 1144
NlaIV GGNNCC 2 cut(s) 676, 897
NmeAIII GCCGAG 1 cut(s) 688
NsiI ATGCAT 1 cut(s) 741
NspI RCATGY 1 cut(s) 739
NspV TTCGAA 1 cut(s) 247
PceI AGGCCT 1 cut(s) 1129
PciSI GCTCTTC 1 cut(s) 976
PctI GAATGC 1 cut(s) 1103
PfeI GAWTC 3 cut(s) 134, 686, 791
PfoI TCCNGGA 1 cut(s) 1060
PkrI GCNGC 4 cut(s) 31, 934, 1103, 1138
PleI GAGTC 1 cut(s) 933
PmeI GTTTAAAC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 988
Psp1406I AACGTT 1 cut(s) 667
Psp6I CCWGG 2 cut(s) 637, 1175
PspGI CCWGG 2 cut(s) 637, 1175
PspN4I GGNNCC 2 cut(s) 676, 897
PspPI GGNCC 5 cut(s) 273, 313, 369, 509, 518
PstI CTGCAG 1 cut(s) 1123
PsuI RGATCY 1 cut(s) 115
SacI GAGCTC 1 cut(s) 988
SapI GCTCTTC 1 cut(s) 976
SaqAI TTAA 7 cut(s) 48, 69, 99, 308, 905, 1023, 1163
SatI GCNGC 4 cut(s) 30, 933, 1102, 1137
Sau3AI GATC 4 cut(s) 115, 150, 474, 604
Sau96I GGNCC 5 cut(s) 273, 313, 369, 509, 518
SchI GAGTC 1 cut(s) 934
ScrFI CCNGG 4 cut(s) 373, 639, 1062, 1177
SduI GDGCHC 3 cut(s) 257, 549, 988
SfaNI GCATC 2 cut(s) 571, 774
SfcI CTRYAG 1 cut(s) 1119
SfuI TTCGAA 1 cut(s) 247
SinI GGWCC 3 cut(s) 273, 313, 518
Sse9I AATT 6 cut(s) 423, 700, 778, 801, 906, 1087
SseBI AGGCCT 1 cut(s) 1129
SspMI CTAG 4 cut(s) 317, 812, 866, 1032
SstI GAGCTC 1 cut(s) 988
StuI AGGCCT 1 cut(s) 1129
StyD4I CCNGG 4 cut(s) 371, 637, 1060, 1175
StyI CCWWGG 3 cut(s) 129, 258, 384
TaaI ACNGT 2 cut(s) 415, 961
TaiI ACGT 2 cut(s) 190, 670
TaqI TCGA 4 cut(s) 247, 477, 607, 789
TasI AATT 6 cut(s) 423, 700, 778, 801, 906, 1087
TfiI GAWTC 3 cut(s) 134, 686, 791
Tru1I TTAA 7 cut(s) 48, 69, 99, 308, 905, 1023, 1163
Tru9I TTAA 7 cut(s) 48, 69, 99, 308, 905, 1023, 1163
TscAI CASTG 3 cut(s) 153, 160, 411
TseI GCWGC 4 cut(s) 29, 932, 1101, 1136
TspDTI ATGAA 6 cut(s) 95, 674, 678, 864, 1017, 1161
TspRI CASTG 3 cut(s) 153, 160, 411
VpaK11BI GGWCC 3 cut(s) 273, 313, 518
XapI RAATTY 1 cut(s) 1087
XbaI TCTAGA 1 cut(s) 865
XceI RCATGY 1 cut(s) 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XspI CTAG 4 cut(s) 317, 812, 866, 1032
ZraI GACGTC 1 cut(s) 188
Zsp2I ATGCAT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.