Rorug02G0221100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016246
Physical Location & Seq
Reverse (-)
21370064 .. 21373171
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0221100.1

Sequence Viewer

Length: 558 bp
ATGGTCACCAACAGCTGTCGCGGTGGCGCGTATACCACCATCAAAGAGCACGTCACCTTCGAAAAGGAGATCAAGAAGAGCAAATTCATTGCCCTCGCCGCTTCCGTCTCCGATGAACAATCCGCTCTCTCCATCCTCTCTCAGGTTAGGGATCCTTGGGCTACTCACAATTGCTGGGCATACAAGGTGGGAGATCAGTACCGGTCTAATGATGATGGTGAGCCATCTGAAACGACCGGGAAACCCATTCATTCTGCTATTGAGTCTTTCGGAATTGATAGAGTAATGGAGCTAGTCATTCGGTATTTTGGAGGAATTAAATTGGGGACTGGAGGACTAGTCAGGGCATATGGAGGAGTAACATCAGAATGCTTGAGAGATGCCCCAACTCATCCTGTAAAATCGAAGATACTCAATAATGGTGAGAAGATAGATGGCCCGGCTAGGGCTGAGGGCCAAATGGGTGGATTTCGTGAGGCACTTGGATACTGCGATCTCCTAGACCTAGGGTTTCAAGGGTCTCGTTCTCAACATGGTGGAATGCCAACACCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.03

Weight (kDa)

6.52

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 25 - 125 3.4e-30 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23630
malus_domestica MD15G1341200.v1.1
prunus_persica Prupe.6G162300_v2.0.a1 Prupe.6G162300_v2.0.a1
pyrus_communis pycom01g02920
rosa_chinensis RchiOBHm_Chr2g0119231
rosa_laevigata RLG00000018457
rosa_multiflora Rmu_sc0004760.1_g000013
rosa_roxburghii Rroxscaffold_2G00124420
rosa_rugosa Rorug02G0221100
rosa_samantha Rh2AG279100 Rh2BG289900 Rh2DG285000 Rh2DG304000
rosa_wichuraiana Rw2G022190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 125
AccI GTMKAC 1 cut(s) 32
AccII CGCG 2 cut(s) 21, 29
AciI CCGC 3 cut(s) 21, 99, 123
AclWI GGATC 2 cut(s) 146, 159
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 142, 445
AgeI ACCGGT 1 cut(s) 201
AgsI TTSAA 1 cut(s) 515
AhlI ACTAGT 1 cut(s) 337
AjiI CACGTC 1 cut(s) 52
AluBI AGCT 2 cut(s) 15, 292
AluI AGCT 2 cut(s) 15, 292
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 2 cut(s) 112, 525
AlwI GGATC 2 cut(s) 146, 159
AoxI GGCC 2 cut(s) 436, 454
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AsiGI ACCGGT 1 cut(s) 201
AspA2I CCTAGG 1 cut(s) 505
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 437, 454
AsuC2I CCSGG 2 cut(s) 238, 440
AsuHPI GGTGA 3 cut(s) 46, 230, 434
AsuII TTCGAA 1 cut(s) 60
AvrII CCTAGG 1 cut(s) 505
BamHI GGATCC 1 cut(s) 151
Bbv12I GWGCWC 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 450
BccI CCATC 5 cut(s) 47, 140, 209, 232, 428
BciVI GTATCC 1 cut(s) 479
BcnI CCSGG 2 cut(s) 238, 440
BcoDI GTCTC 2 cut(s) 112, 525
BcuI ACTAGT 1 cut(s) 337
BfaI CTAG 5 cut(s) 293, 338, 444, 500, 506
BfuI GTATCC 1 cut(s) 479
BisI GCNGC 1 cut(s) 99
BlnI CCTAGG 1 cut(s) 505
BlsI GCNGC 1 cut(s) 100
Bme1390I CCNGG 2 cut(s) 238, 440
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 437, 454
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 238, 440
BmsI GCATC 1 cut(s) 370
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 394
BpuMI CCSGG 2 cut(s) 238, 440
BsaI GGTCTC 1 cut(s) 525
BsaJI CCNNGG 2 cut(s) 155, 505
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 445
Bse118I RCCGGY 1 cut(s) 201
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 155, 505
BseGI GGATG 2 cut(s) 132, 391
BseLI CCNNNNNNNGG 2 cut(s) 142, 445
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 2 cut(s) 155, 441
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 369
BseYI CCCAGC 1 cut(s) 174
Bsh1236I CGCG 2 cut(s) 21, 29
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 2 cut(s) 438, 456
BshTI ACCGGT 1 cut(s) 201
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 3 cut(s) 202, 237, 440
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 2 cut(s) 142, 445
BsmAI GTCTC 2 cut(s) 112, 525
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 2 cut(s) 374, 546
BsnI GGCC 2 cut(s) 438, 456
Bso31I GGTCTC 1 cut(s) 525
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 4 cut(s) 69, 151, 193, 493
BspACI CCGC 3 cut(s) 21, 99, 123
BspANI GGCC 2 cut(s) 438, 456
BspCNI CTCAG 2 cut(s) 154, 442
BspFNI CGCG 2 cut(s) 21, 29
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 2 cut(s) 146, 159
BspQI GCTCTTC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 525
BsrBI CCGCTC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 201
BsrI ACTGG 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 2 cut(s) 155, 505
BssMI GATC 4 cut(s) 69, 151, 193, 493
BssNAI GTATAC 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 155, 505
Bst1107I GTATAC 1 cut(s) 33
Bst6I CTCTTC 1 cut(s) 71
BstBI TTCGAA 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 141, 450
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 2 cut(s) 132, 391
BstFNI CGCG 2 cut(s) 21, 29
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 4 cut(s) 72, 154, 196, 496
BstMAI GTCTC 2 cut(s) 112, 525
BstMBI GATC 4 cut(s) 69, 151, 193, 493
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 2 cut(s) 236, 438
BstUI CGCG 2 cut(s) 21, 29
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 1 cut(s) 151
BstZ17I GTATAC 1 cut(s) 33
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 2 cut(s) 438, 456
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 2 cut(s) 132, 391
CfoI GCGC 1 cut(s) 29
Cfr10I RCCGGY 1 cut(s) 201
Cfr13I GGNCC 2 cut(s) 437, 454
Csp6I GTAC 1 cut(s) 199
CspAI ACCGGT 1 cut(s) 201
CviAII CATG 1 cut(s) 533
CviJI RGCY 8 cut(s) 15, 161, 223, 292, 438, 443, 449, 456
CviKI_1 RGCY 8 cut(s) 15, 161, 223, 292, 438, 443, 449, 456
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 2 cut(s) 141, 450
DpnI GATC 4 cut(s) 71, 153, 195, 495
DpnII GATC 4 cut(s) 69, 151, 193, 493
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 155, 505
Eco31I GGTCTC 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 4
EcoT14I CCWWGG 2 cut(s) 155, 505
ErhI CCWWGG 2 cut(s) 155, 505
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 1 cut(s) 536
FaiI YATR 5 cut(s) 33, 181, 349, 351, 534
FaqI GGGAC 1 cut(s) 340
FatI CATG 1 cut(s) 532
FauNDI CATATG 1 cut(s) 349
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 1 cut(s) 99
FokI GGATG 2 cut(s) 119, 378
Fsp4HI GCNGC 1 cut(s) 99
FspBI CTAG 5 cut(s) 293, 338, 444, 500, 506
GlaI GCGC 1 cut(s) 28
GluI GCNGC 1 cut(s) 99
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 2 cut(s) 438, 456
HapII CCGG 3 cut(s) 202, 237, 440
HhaI GCGC 1 cut(s) 29
Hin1II CATG 1 cut(s) 536
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HinfI GANTC 1 cut(s) 263
HpaII CCGG 3 cut(s) 202, 237, 440
HphI GGTGA 3 cut(s) 46, 230, 434
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 4 cut(s) 112, 229, 272, 367
Hpy188III TCNNGA 2 cut(s) 73, 473
Hpy8I GTNNAC 1 cut(s) 33
HpyAV CCTTC 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 51
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 2 cut(s) 141, 450
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 1 cut(s) 536
HspAI GCGC 1 cut(s) 27
Kzo9I GATC 4 cut(s) 69, 151, 193, 493
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 8 cut(s) 128, 160, 215, 250, 315, 328, 408, 453
LweI GCATC 1 cut(s) 370
MaeI CTAG 5 cut(s) 293, 338, 444, 500, 506
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 3 cut(s) 4, 52, 358
MalI GATC 4 cut(s) 71, 153, 195, 495
MbiI CCGCTC 1 cut(s) 125
MboI GATC 4 cut(s) 69, 151, 193, 493
MboII GAAGA 3 cut(s) 88, 418, 439
MfeI CAATTG 1 cut(s) 169
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 5 cut(s) 83, 169, 273, 315, 320
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 7 cut(s) 104, 146, 305, 326, 347, 445, 469
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 1 cut(s) 367
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 3 cut(s) 202, 237, 440
MspR9I CCNGG 2 cut(s) 238, 440
MunI CAATTG 1 cut(s) 169
Mva1269I GAATGC 2 cut(s) 374, 546
MvnI CGCG 2 cut(s) 21, 29
MwoI GCNNNNNNNGC 1 cut(s) 98
NciI CCSGG 2 cut(s) 238, 440
NdeI CATATG 1 cut(s) 349
NdeII GATC 4 cut(s) 69, 151, 193, 493
NlaIII CATG 1 cut(s) 536
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 2 cut(s) 4, 52
NspV TTCGAA 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 102
PctI GAATGC 2 cut(s) 374, 546
PinAI ACCGGT 1 cut(s) 201
PkrI GCNGC 1 cut(s) 100
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PspEI GGTNACC 1 cut(s) 4
PspFI CCCAGC 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 437, 454
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 15
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 367
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 4 cut(s) 69, 151, 193, 493
Sau96I GGNCC 2 cut(s) 437, 454
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 2 cut(s) 238, 440
SduI GDGCHC 1 cut(s) 51
SetI ASST 7 cut(s) 17, 54, 59, 147, 189, 294, 507
SfaNI GCATC 1 cut(s) 370
SfuI TTCGAA 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 367
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
SpeI ACTAGT 1 cut(s) 337
Sse9I AATT 5 cut(s) 83, 169, 273, 315, 320
SsiI CCGC 3 cut(s) 21, 99, 123
SspMI CTAG 5 cut(s) 293, 338, 444, 500, 506
StyD4I CCNGG 2 cut(s) 236, 438
StyI CCWWGG 2 cut(s) 155, 505
TaiI ACGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 60, 404
TasI AATT 5 cut(s) 83, 169, 273, 315, 320
TauI GCSGC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TseFI GTSAC 2 cut(s) 4, 52
Tsp45I GTSAC 2 cut(s) 4, 52
TspDTI ATGAA 3 cut(s) 76, 129, 239
TspGWI ACGGA 1 cut(s) 94
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 83
XmaJI CCTAGG 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 32
XspI CTAG 5 cut(s) 293, 338, 444, 500, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.