Rorug02G0221300

NYN domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013218
Physical Location & Seq
Reverse (-)
21393854 .. 21395660
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0221300.1

Sequence Viewer

Length: 774 bp
ATGATAAAATCAAATCAAATGCCAGCAGCAGCAGCAATCACACCAATACCTAATTTCCATTTCCATTCCCACAAGGTAGCTCCTATTAGGTTTCGTCTCTGGGCCTCGATTCTCAGCATTGCCGCAGTAGCCAACCGCGCCACCATGGTCACCGCCGCCACCAGCAGCAGCTGTCGCGGTGGCGCGTACACCACCATCAAAGAGCGCGTCACCTTCGAAAAAGAGATCAAGAAGAGCAAATTCATCGCCCTCGCCGCTTCCGTCTCCGACGAACAATCCGCTTTCTCCTTCCTCTCTCAGGTTAGGGATCCTCGTGCTACTCACAATTGCTGGGCGTACAAGGTGGGAGATCAGTACCGGTCTAATGATGATGGTGAGCCATCTGGAACGGCTGGGAAGCCCATTCATTCTGCTATTGAGTCTTCAGGAATTGATAGAGTAATGGTGGTGGTCATCCGGTATTTTGGAGGAATTAAATTGGGGACTGGAGGACTAGTCAGGGCATATGGAGGAGTAACATCGGAATGCTTGAGAAATGCCCCAACTCATCTCGTAAAATCGAAGGTAAGAATGGGTGTAGAGGTTCCATTTGATCTTATCGGCATTCTATACCATCAGCTACAATCTTTTCAAGTTGAAGACATCAAACAAGATTATGAGACCGGTAAGGATGGTATCACCATGGTTACCTTTCAAGTTGATTTTGACCGTGTTGATAAATTAGAGGATGCTATCAAAAACAACTGTAACCGGGAGTTGGTGTTCTACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN OHA OST-HTH

Protein Analysis

257

Amino Acids

28.39

Weight (kDa)

9.1

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 77 - 177 1e-34 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09840 AT5G64710 AT5G64710
fragaria_vesca FvH4_1g23660
malus_domestica MD01G1017000.v1.1
prunus_persica Prupe.7G207600_v2.0.a1
pyrus_communis pycom01g03040
rosa_chinensis RchiOBHm_Chr2g0119261
rosa_laevigata RLG00000018460
rosa_multiflora Rmu_sc0004760.1_g000002
rosa_roxburghii Rroxscaffold_2G00124380
rosa_rugosa Rorug02G0221300 Rorug02G0221300
rosa_samantha Rh2AG279400 Rh2BG290100 Rh2DG285400 Rh2DG304400
rosa_wichuraiana Rw2G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 138, 177, 185, 207
AciI CCGC 7 cut(s) 123, 136, 153, 156, 177, 255, 279
AclWI GGATC 2 cut(s) 302, 315
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 3 cut(s) 188, 338, 356
AfiI CCNNNNNNNGG 2 cut(s) 298, 757
AgeI ACCGGT 2 cut(s) 357, 662
AgsI TTSAA 3 cut(s) 632, 638, 695
AhlI ACTAGT 1 cut(s) 493
AloI GAACNNNNNNTCC 1 cut(s) 746
AluBI AGCT 3 cut(s) 80, 171, 619
AluI AGCT 3 cut(s) 80, 171, 619
Alw26I GTCTC 3 cut(s) 101, 268, 653
AlwI GGATC 2 cut(s) 302, 315
AlwNI CAGNNNCTG 1 cut(s) 171
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 5 cut(s) 26, 29, 32, 165, 168
ApoI RAATTY 1 cut(s) 239
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AsiGI ACCGGT 2 cut(s) 357, 662
AspLEI GCGC 3 cut(s) 140, 185, 207
AspS9I GGNCC 1 cut(s) 102
AsuC2I CCSGG 1 cut(s) 752
AsuHPI GGTGA 4 cut(s) 142, 202, 386, 670
AsuII TTCGAA 1 cut(s) 216
BamHI GGATCC 1 cut(s) 307
BauI CACGAG 1 cut(s) 312
BbsI GAAGAC 2 cut(s) 414, 645
BbvI GCAGC 5 cut(s) 38, 41, 44, 177, 180
BccI CCATC 5 cut(s) 203, 365, 388, 621, 665
BceAI ACGGC 1 cut(s) 405
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BcnI CCSGG 1 cut(s) 752
BcoDI GTCTC 3 cut(s) 101, 268, 653
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 1 cut(s) 494
BisI GCNGC 8 cut(s) 27, 30, 33, 123, 156, 166, 169, 255
BlsI GCNGC 8 cut(s) 28, 31, 34, 124, 157, 167, 170, 256
Bme1390I CCNGG 1 cut(s) 752
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 2 cut(s) 309, 585
BmrFI CCNGG 1 cut(s) 752
BmsI GCATC 1 cut(s) 718
BpiI GAAGAC 2 cut(s) 414, 645
BpmI CTGGAG 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 752
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 2 cut(s) 144, 681
BsaWI WCCGGW 3 cut(s) 357, 456, 662
BsaXI ACNNNNNCTCC 3 cut(s) 480, 510, 746
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 757
Bse118I RCCGGY 2 cut(s) 357, 662
Bse1I ACTGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 144, 681
BseGI GGATG 3 cut(s) 453, 676, 733
BseLI CCNNNNNNNGG 2 cut(s) 298, 757
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 2 cut(s) 127, 311
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 525
BseXI GCAGC 5 cut(s) 38, 41, 44, 177, 180
BseYI CCCAGC 2 cut(s) 330, 392
Bsh1236I CGCG 4 cut(s) 138, 177, 185, 207
BshFI GGCC 1 cut(s) 104
BshTI ACCGGT 2 cut(s) 357, 662
BsiSI CCGG 4 cut(s) 358, 457, 663, 751
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 2 cut(s) 298, 757
BsmAI GTCTC 3 cut(s) 101, 268, 653
BsmBI CGTCTC 2 cut(s) 101, 268
BsmFI GGGAC 1 cut(s) 496
BsmI GAATGC 2 cut(s) 530, 603
BsnI GGCC 1 cut(s) 104
Bso31I GGTCTC 1 cut(s) 653
Bsp119I TTCGAA 1 cut(s) 216
Bsp143I GATC 4 cut(s) 225, 307, 349, 592
Bsp19I CCATGG 2 cut(s) 144, 681
BspACI CCGC 7 cut(s) 123, 136, 153, 156, 177, 255, 279
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 126, 310
BspFNI CGCG 4 cut(s) 138, 177, 185, 207
BspLI GGNNCC 2 cut(s) 309, 585
BspPI GGATC 2 cut(s) 302, 315
BspQI GCTCTTC 1 cut(s) 227
BspT104I TTCGAA 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 653
BsrDI GCAATG 1 cut(s) 117
BsrFI RCCGGY 2 cut(s) 357, 662
BsrI ACTGG 1 cut(s) 490
BssAI RCCGGY 2 cut(s) 357, 662
BssECI CCNNGG 2 cut(s) 144, 681
BssMI GATC 4 cut(s) 225, 307, 349, 592
BssSI CACGAG 1 cut(s) 312
BssT1I CCWWGG 2 cut(s) 144, 681
Bst2BI CACGAG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 710, 746
Bst6I CTCTTC 1 cut(s) 227
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 2 cut(s) 113, 297
BstDSI CCRYGG 2 cut(s) 144, 681
BstEII GGTNACC 2 cut(s) 148, 685
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 3 cut(s) 453, 676, 733
BstFNI CGCG 4 cut(s) 138, 177, 185, 207
BstHHI GCGC 3 cut(s) 140, 185, 207
BstKTI GATC 4 cut(s) 228, 310, 352, 595
BstMAI GTCTC 3 cut(s) 101, 268, 653
BstMBI GATC 4 cut(s) 225, 307, 349, 592
BstMWI GCNNNNNNNGC 5 cut(s) 32, 128, 137, 174, 254
BstPI GGTNACC 2 cut(s) 148, 685
BstSCI CCNGG 1 cut(s) 750
BstUI CGCG 4 cut(s) 138, 177, 185, 207
BstV1I GCAGC 5 cut(s) 38, 41, 44, 177, 180
BstV2I GAAGAC 2 cut(s) 414, 645
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 1 cut(s) 104
BtgI CCRYGG 2 cut(s) 144, 681
BtgZI GCGATG 1 cut(s) 229
BtsCI GGATG 3 cut(s) 453, 676, 733
Cac8I GCNNGC 1 cut(s) 24
CaiI CAGNNNCTG 1 cut(s) 171
CfoI GCGC 3 cut(s) 140, 185, 207
Cfr10I RCCGGY 2 cut(s) 357, 662
Cfr13I GGNCC 1 cut(s) 102
CseI GACGC 1 cut(s) 196
Csp6I GTAC 3 cut(s) 187, 337, 355
CspAI ACCGGT 2 cut(s) 357, 662
CviAII CATG 2 cut(s) 145, 682
CviJI RGCY 8 cut(s) 80, 104, 131, 171, 379, 392, 400, 619
CviKI_1 RGCY 8 cut(s) 80, 104, 131, 171, 379, 392, 400, 619
CviQI GTAC 3 cut(s) 187, 337, 355
DdeI CTNAG 2 cut(s) 113, 297
DpnI GATC 4 cut(s) 227, 309, 351, 594
DpnII GATC 4 cut(s) 225, 307, 349, 592
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 2 cut(s) 144, 681
Eco31I GGTCTC 1 cut(s) 653
Eco57I CTGAAG 1 cut(s) 408
Eco91I GGTNACC 2 cut(s) 148, 685
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoO65I GGTNACC 2 cut(s) 148, 685
EcoT14I CCWWGG 2 cut(s) 144, 681
ErhI CCWWGG 2 cut(s) 144, 681
Esp3I CGTCTC 2 cut(s) 101, 268
FaeI CATG 2 cut(s) 148, 685
FaiI YATR 6 cut(s) 146, 505, 507, 610, 657, 683
FaqI GGGAC 1 cut(s) 496
FatI CATG 2 cut(s) 144, 681
FauNDI CATATG 1 cut(s) 505
Fnu4HI GCNGC 8 cut(s) 27, 30, 33, 123, 156, 166, 169, 255
FokI GGATG 3 cut(s) 440, 683, 740
Fsp4HI GCNGC 8 cut(s) 27, 30, 33, 123, 156, 166, 169, 255
FspBI CTAG 1 cut(s) 494
GlaI GCGC 3 cut(s) 139, 184, 206
GluI GCNGC 8 cut(s) 27, 30, 33, 123, 156, 166, 169, 255
GsaI CCCAGC 2 cut(s) 334, 396
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 1 cut(s) 104
HapII CCGG 4 cut(s) 358, 457, 663, 751
HgaI GACGC 1 cut(s) 196
HhaI GCGC 3 cut(s) 140, 185, 207
Hin1II CATG 2 cut(s) 148, 685
Hin6I GCGC 3 cut(s) 138, 183, 205
HinP1I GCGC 3 cut(s) 138, 183, 205
HinfI GANTC 2 cut(s) 109, 419
HpaII CCGG 4 cut(s) 358, 457, 663, 751
HphI GGTGA 4 cut(s) 142, 202, 386, 670
Hpy166II GTNNAC 1 cut(s) 189
Hpy188I TCNGA 2 cut(s) 268, 523
Hpy188III TCNNGA 3 cut(s) 229, 384, 426
Hpy8I GTNNAC 1 cut(s) 189
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 3 cut(s) 223, 298, 556
HpyCH4III ACNGT 2 cut(s) 710, 746
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 128, 137, 174, 254
HpyF3I CTNAG 2 cut(s) 113, 297
Hsp92II CATG 2 cut(s) 148, 685
HspAI GCGC 3 cut(s) 138, 183, 205
Kzo9I GATC 4 cut(s) 225, 307, 349, 592
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 1 cut(s) 85
Lsp1109I GCAGC 5 cut(s) 38, 41, 44, 177, 180
LweI GCATC 1 cut(s) 718
MaeI CTAG 1 cut(s) 494
MaeIII GTNAC 5 cut(s) 148, 208, 514, 685, 746
MalI GATC 4 cut(s) 227, 309, 351, 594
MboI GATC 4 cut(s) 225, 307, 349, 592
MboII GAAGA 3 cut(s) 244, 414, 650
MfeI CAATTG 1 cut(s) 325
MflI RGATCY 1 cut(s) 307
MluCI AATT 7 cut(s) 52, 239, 325, 429, 471, 476, 719
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 9 cut(s) 115, 260, 302, 321, 461, 482, 503, 574, 718
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 523
MspA1I CMGCKG 1 cut(s) 171
MspI CCGG 4 cut(s) 358, 457, 663, 751
MspR9I CCNGG 1 cut(s) 752
MunI CAATTG 1 cut(s) 325
Mva1269I GAATGC 2 cut(s) 530, 603
MvnI CGCG 4 cut(s) 138, 177, 185, 207
MwoI GCNNNNNNNGC 5 cut(s) 32, 128, 137, 174, 254
NciI CCSGG 1 cut(s) 752
NcoI CCATGG 2 cut(s) 144, 681
NdeI CATATG 1 cut(s) 505
NdeII GATC 4 cut(s) 225, 307, 349, 592
NlaIII CATG 2 cut(s) 148, 685
NlaIV GGNNCC 2 cut(s) 309, 585
NmuCI GTSAC 2 cut(s) 148, 208
NspV TTCGAA 1 cut(s) 216
PciSI GCTCTTC 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 2 cut(s) 530, 603
PfeI GAWTC 1 cut(s) 109
PinAI ACCGGT 2 cut(s) 357, 662
PkrI GCNGC 8 cut(s) 28, 31, 34, 124, 157, 167, 170, 256
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PspEI GGTNACC 2 cut(s) 148, 685
PspFI CCCAGC 2 cut(s) 330, 392
PspN4I GGNNCC 2 cut(s) 309, 585
PspPI GGNCC 1 cut(s) 102
PstNI CAGNNNCTG 1 cut(s) 171
PsuI RGATCY 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 171
RsaI GTAC 3 cut(s) 188, 338, 356
RsaNI GTAC 3 cut(s) 187, 337, 355
RseI CAYNNNNRTG 1 cut(s) 523
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 8 cut(s) 27, 30, 33, 123, 156, 166, 169, 255
Sau3AI GATC 4 cut(s) 225, 307, 349, 592
Sau96I GGNCC 1 cut(s) 102
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 752
SfaNI GCATC 1 cut(s) 718
SfuI TTCGAA 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 523
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 7 cut(s) 52, 239, 325, 429, 471, 476, 719
SsiI CCGC 7 cut(s) 123, 136, 153, 156, 177, 255, 279
SspMI CTAG 1 cut(s) 494
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 2 cut(s) 144, 681
TaaI ACNGT 2 cut(s) 710, 746
TaqI TCGA 3 cut(s) 107, 216, 560
TasI AATT 7 cut(s) 52, 239, 325, 429, 471, 476, 719
TauI GCSGC 3 cut(s) 125, 158, 257
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TseFI GTSAC 2 cut(s) 148, 208
TseI GCWGC 5 cut(s) 26, 29, 32, 165, 168
Tsp45I GTSAC 2 cut(s) 148, 208
TspDTI ATGAA 2 cut(s) 232, 395
TspGWI ACGGA 1 cut(s) 250
XagI CCTNNNNNAGG 1 cut(s) 296
XapI RAATTY 1 cut(s) 239
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.