Rorug02G0495700

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011188
Physical Location & Seq
Reverse (-)
62584253 .. 62585580
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0495700.1

Sequence Viewer

Length: 414 bp
ATGGCCGAAGCGAAAAGGGGTGAAGATGTTACGAGCGTTGATGTATTTACTGCAAAAGGTCTGCTCAGCACCGGTCACCTGTATCTGGATGTCAGGACGAATGAGGAGTTCAACACGAGCCACGTTGAAAATGCCTTCAATGTTCCTTACATATTCAAGACCCAAGAAGGTAAAGTGAAAAATCCTGAATTTCTCACCCATATTTCCTCAATACTCAAGAAACAGGATCACTTAGTCGTGGGTTGTAACAGCGGAGGCAGATCACTTAAAGCTTGTGTAGATCTTCTTAATGCAGGTTTCGAGCATGTCACTAACATGGAAGGGGGCTACTCTTCATGGGTGGACAGTGGACTTGCTGCTGACAAAGACAAACCATCTGGTGAATTAAAAGTCGCTTGCAAGTTCCGCCCTTGA

Protein Analysis

137

Amino Acids

14.99

Weight (kDa)

6.19

Isoelectric Point (pI)

25.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 21 - 115 9e-17 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03400 AT4G03400
fragaria_vesca FvH4_6g44990 FvH4_6g44990
malus_domestica MD17G1081000.v1.1
prunus_persica Prupe.3G233900_v2.0.a1
pyrus_communis pycom09g01640
rosa_chinensis RchiOBHm_Chr2g0162261
rosa_laevigata RLG00000021331
rosa_multiflora Rmu_sc0004020.1_g000022
rosa_roxburghii Rroxscaffold_2G00088210
rosa_rugosa Rorug02G0495700 Rorug02G0495800 Rorug02G0495900 Rorug02G0496000
rosa_samantha Rh2AG562000 Rh2BG575100 Rh2CG545300 Rh2DG585000
rosa_wichuraiana Rw2G046540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 284
AciI CCGC 2 cut(s) 252, 406
AclWI GGATC 1 cut(s) 234
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 188
AfiI CCNNNNNNNGG 1 cut(s) 85
AgeI ACCGGT 1 cut(s) 71
AgsI TTSAA 4 cut(s) 112, 128, 139, 157
AluBI AGCT 1 cut(s) 272
AluI AGCT 1 cut(s) 272
AlwI GGATC 1 cut(s) 234
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 188
AsiGI ACCGGT 1 cut(s) 71
AsuHPI GGTGA 4 cut(s) 32, 68, 187, 392
BauI CACGAG 1 cut(s) 115
BbvI GCAGC 1 cut(s) 343
BccI CCATC 1 cut(s) 382
BfuAI ACCTGC 1 cut(s) 284
BglII AGATCT 1 cut(s) 280
BisI GCNGC 1 cut(s) 357
BlpI GCTNAGC 1 cut(s) 65
BlsI GCNGC 1 cut(s) 358
Bpu1102I GCTNAGC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse118I RCCGGY 1 cut(s) 71
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMII CTCAG 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 1 cut(s) 343
BshFI GGCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 71
BsiSI CCGG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 85
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 3 cut(s) 226, 260, 280
Bsp1720I GCTNAGC 1 cut(s) 65
BspACI CCGC 2 cut(s) 252, 406
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 78
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 71
BssMI GATC 3 cut(s) 226, 260, 280
BssSI CACGAG 1 cut(s) 115
Bst2BI CACGAG 1 cut(s) 115
Bst4CI ACNGT 1 cut(s) 347
Bst6I CTCTTC 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 2 cut(s) 65, 232
BstEII GGTNACC 1 cut(s) 74
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 3 cut(s) 229, 263, 283
BstMBI GATC 3 cut(s) 226, 260, 280
BstMWI GCNNNNNNNGC 1 cut(s) 405
BstNSI RCATGY 1 cut(s) 308
BstPI GGTNACC 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 343
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 94
BtsIMutI CAGTG 1 cut(s) 352
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 71
CspAI ACCGGT 1 cut(s) 71
CviAII CATG 3 cut(s) 305, 316, 336
CviJI RGCY 4 cut(s) 5, 120, 272, 327
CviKI_1 RGCY 4 cut(s) 5, 120, 272, 327
DdeI CTNAG 2 cut(s) 65, 232
DpnI GATC 3 cut(s) 228, 262, 282
DpnII GATC 3 cut(s) 226, 260, 280
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 337
EciI GGCGGA 1 cut(s) 395
Eco91I GGTNACC 1 cut(s) 74
EcoO65I GGTNACC 1 cut(s) 74
FaeI CATG 3 cut(s) 308, 319, 339
FaiI YATR 5 cut(s) 152, 201, 306, 317, 337
FatI CATG 3 cut(s) 304, 315, 335
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 357
GluI GCNGC 1 cut(s) 357
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 72
Hin1II CATG 3 cut(s) 308, 319, 339
HindIII AAGCTT 1 cut(s) 270
HpaII CCGG 1 cut(s) 72
HphI GGTGA 4 cut(s) 32, 68, 187, 392
Hpy166II GTNNAC 2 cut(s) 343, 350
Hpy188III TCNNGA 5 cut(s) 86, 94, 157, 185, 217
Hpy8I GTNNAC 2 cut(s) 343, 350
HpyAV CCTTC 3 cut(s) 145, 161, 314
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 3 cut(s) 53, 293, 399
HpyF10VI GCNNNNNNNGC 1 cut(s) 405
HpyF3I CTNAG 2 cut(s) 65, 232
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 3 cut(s) 308, 319, 339
Kzo9I GATC 3 cut(s) 226, 260, 280
LpnPI CCDG 8 cut(s) 71, 79, 85, 92, 198, 209, 279, 363
Lsp1109I GCAGC 1 cut(s) 343
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 4 cut(s) 28, 74, 245, 307
MalI GATC 3 cut(s) 228, 262, 282
MboI GATC 3 cut(s) 226, 260, 280
MboII GAAGA 3 cut(s) 35, 275, 324
MflI RGATCY 1 cut(s) 280
MluCI AATT 2 cut(s) 188, 383
MnlI CCTC 3 cut(s) 97, 217, 248
MseI TTAA 3 cut(s) 267, 288, 386
MslI CAYNNNNRTG 1 cut(s) 314
MspA1I CMGCKG 1 cut(s) 252
MspI CCGG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 405
NdeII GATC 3 cut(s) 226, 260, 280
NlaIII CATG 3 cut(s) 308, 319, 339
NmuCI GTSAC 2 cut(s) 74, 307
NspI RCATGY 1 cut(s) 308
PinAI ACCGGT 1 cut(s) 71
PkrI GCNGC 1 cut(s) 358
PspEI GGTNACC 1 cut(s) 74
PsuI RGATCY 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 314
SaqAI TTAA 3 cut(s) 267, 288, 386
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 3 cut(s) 226, 260, 280
SetI ASST 6 cut(s) 61, 81, 126, 172, 274, 298
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 188, 383
SsiI CCGC 2 cut(s) 252, 406
TaaI ACNGT 1 cut(s) 347
TaiI ACGT 1 cut(s) 126
TaqI TCGA 1 cut(s) 300
TasI AATT 2 cut(s) 188, 383
Tru1I TTAA 3 cut(s) 267, 288, 386
Tru9I TTAA 3 cut(s) 267, 288, 386
TscAI CASTG 1 cut(s) 352
TseFI GTSAC 2 cut(s) 74, 307
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 74, 307
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 352
XapI RAATTY 1 cut(s) 188
XceI RCATGY 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.