Rorug02G0496000

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011188
Physical Location & Seq
Forward (+)
62602316 .. 62603141
826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0496000.1

Sequence Viewer

Length: 618 bp
ATGGCCTATTTCCAGCTCTTCACCATCCTTGCCATATTAGCAATTTTTTCCCCATCAATTTCGGCCAAAAATTATGTTGTTGGGGACGAACAAGGCTGGACCGTTGAAATTGACTGTGAAGCTTGGGCTAAGGGAAAGCAATTTTATGTTGGTGACAACCTTATTTTCAAGTATCTACCAGGAGTTCATAACGTGGTTGAAGTGAAGGAAAAAGAGTTTAAGGAATGTGCAGCTCCTTCTAGCACCAAGCCATTAACAACTGGAGGGGATTTGATCAAGCTTGCAACCCCAGGAAAGAAATGGTTCATTTGTAGTGTTGGTAAGCATTGTGAATTGGGGAACCAGAAGGTTGCTGTAACTGTTTTGTCATCACCTTCCTCTTCTCCTAGCTCAATCCCAAGCAGCTCAAGTCCAAATGTTGCCCCAAGTCCGAGTACCTCAAGCCTAACTTCCCCAGGCCCAGATGCCTCAAGCCAGAGTGAACCAAGCCCAAGTATCTCAAGCCCGAGTGGACCAAGCCCGAGTACCTCAAGCCCGAGTACCTCTGCGGCAACTGTCGGAAAGATATATGGGTGGCTGGTAATTGTTAGTGGCATTGTAGGGTTTCACATGATTTAA

Protein Analysis

205

Amino Acids

21.4

Weight (kDa)

6.81

Isoelectric Point (pI)

62.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 33 - 113 2.4e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03400 AT4G03400
fragaria_vesca FvH4_6g44990 FvH4_6g44990
malus_domestica MD17G1081000.v1.1
prunus_persica Prupe.3G233900_v2.0.a1
pyrus_communis pycom09g01640
rosa_chinensis RchiOBHm_Chr2g0162261
rosa_laevigata RLG00000021331
rosa_multiflora Rmu_sc0004020.1_g000022
rosa_roxburghii Rroxscaffold_2G00088210
rosa_rugosa Rorug02G0495700 Rorug02G0495800 Rorug02G0495900 Rorug02G0496000
rosa_samantha Rh2AG562000 Rh2BG575100 Rh2CG545300 Rh2DG585000
rosa_wichuraiana Rw2G046540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 548
AcoI YGGCCR 1 cut(s) 63
AfaI GTAC 3 cut(s) 436, 526, 541
AgsI TTSAA 3 cut(s) 107, 169, 200
AjnI CCWGG 3 cut(s) 178, 289, 454
AluBI AGCT 6 cut(s) 16, 122, 233, 280, 390, 405
AluI AGCT 6 cut(s) 16, 122, 233, 280, 390, 405
Ama87I CYCGRG 3 cut(s) 505, 520, 535
AoxI GGCC 3 cut(s) 3, 63, 457
ApeKI GCWGC 2 cut(s) 230, 402
Asp700I GAANNNNTTC 1 cut(s) 302
AspS9I GGNCC 3 cut(s) 99, 458, 512
AsuHPI GGTGA 3 cut(s) 13, 164, 363
AvaI CYCGRG 3 cut(s) 505, 520, 535
AvaII GGWCC 2 cut(s) 99, 512
BbvI GCAGC 2 cut(s) 242, 414
BccI CCATC 2 cut(s) 32, 61
BciT130I CCWGG 3 cut(s) 180, 291, 456
BclI TGATCA 1 cut(s) 273
BfaI CTAG 2 cut(s) 240, 387
BisI GCNGC 3 cut(s) 231, 403, 549
BlsI GCNGC 3 cut(s) 232, 404, 550
Bme1390I CCNGG 3 cut(s) 180, 291, 456
Bme18I GGWCC 2 cut(s) 99, 512
BmeT110I CYCGRG 3 cut(s) 505, 520, 535
BmgT120I GGNCC 3 cut(s) 99, 458, 512
BmiI GGNNCC 1 cut(s) 341
BmrFI CCNGG 3 cut(s) 180, 291, 456
BmsI GCATC 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 129
BpuEI CTTGAG 5 cut(s) 391, 424, 454, 484, 514
BsaJI CCNNGG 2 cut(s) 289, 454
Bse1I ACTGG 1 cut(s) 265
BseBI CCWGG 3 cut(s) 180, 291, 456
BseDI CCNNGG 2 cut(s) 289, 454
BseGI GGATG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 265
BseXI GCAGC 2 cut(s) 242, 414
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 3 cut(s) 5, 65, 459
BsiHKCI CYCGRG 3 cut(s) 505, 520, 535
BslFI GGGAC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 3 cut(s) 5, 65, 459
BsoBI CYCGRG 3 cut(s) 505, 520, 535
Bsp143I GATC 1 cut(s) 273
BspACI CCGC 1 cut(s) 548
BspANI GGCC 3 cut(s) 5, 65, 459
BspLI GGNNCC 1 cut(s) 341
BspQI GCTCTTC 1 cut(s) 23
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 2 cut(s) 289, 454
BssMI GATC 1 cut(s) 273
Bst2UI CCWGG 3 cut(s) 180, 291, 456
Bst4CI ACNGT 4 cut(s) 103, 116, 361, 556
Bst6I CTCTTC 2 cut(s) 23, 385
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 24
BstKTI GATC 1 cut(s) 276
BstMBI GATC 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNI CCWGG 3 cut(s) 180, 291, 456
BstSCI CCNGG 3 cut(s) 178, 289, 454
BstV1I GCAGC 2 cut(s) 242, 414
BsuRI GGCC 3 cut(s) 5, 65, 459
BtsCI GGATG 1 cut(s) 24
Cac8I GCNNGC 1 cut(s) 282
Cfr13I GGNCC 3 cut(s) 99, 458, 512
Csp6I GTAC 3 cut(s) 435, 525, 540
CviAII CATG 1 cut(s) 610
CviQI GTAC 3 cut(s) 435, 525, 540
DdeI CTNAG 1 cut(s) 129
DpnI GATC 1 cut(s) 275
DpnII GATC 1 cut(s) 273
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 23, 385
EarI CTCTTC 2 cut(s) 23, 385
Eco47I GGWCC 2 cut(s) 99, 512
Eco88I CYCGRG 3 cut(s) 505, 520, 535
EcoRII CCWGG 3 cut(s) 178, 289, 454
FaeI CATG 1 cut(s) 613
FaiI YATR 7 cut(s) 35, 75, 147, 189, 568, 570, 611
FalI AAGNNNNNCTT 2 cut(s) 433, 465
FaqI GGGAC 1 cut(s) 98
FatI CATG 1 cut(s) 609
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 3 cut(s) 231, 403, 549
FokI GGATG 1 cut(s) 11
Fsp4HI GCNGC 3 cut(s) 231, 403, 549
FspBI CTAG 2 cut(s) 240, 387
GluI GCNGC 3 cut(s) 231, 403, 549
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 3 cut(s) 5, 65, 459
Hin1II CATG 1 cut(s) 613
HindIII AAGCTT 2 cut(s) 120, 278
HphI GGTGA 3 cut(s) 13, 164, 363
Hpy166II GTNNAC 2 cut(s) 482, 512
Hpy188I TCNGA 2 cut(s) 432, 560
Hpy8I GTNNAC 2 cut(s) 482, 512
HpyAV CCTTC 4 cut(s) 199, 246, 340, 384
HpyCH4III ACNGT 4 cut(s) 103, 116, 361, 556
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 230, 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 1 cut(s) 613
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 1 cut(s) 273
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 1 cut(s) 238
Lsp1109I GCAGC 2 cut(s) 242, 414
LweI GCATC 1 cut(s) 454
MaeI CTAG 2 cut(s) 240, 387
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 152, 355
MalI GATC 1 cut(s) 275
MboI GATC 1 cut(s) 273
MboII GAAGA 2 cut(s) 10, 372
MluCI AATT 7 cut(s) 42, 57, 70, 108, 140, 332, 582
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 6 cut(s) 257, 388, 448, 478, 538, 553
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 3 cut(s) 219, 254, 616
MspR9I CCNGG 3 cut(s) 180, 291, 456
MvaI CCWGG 3 cut(s) 180, 291, 456
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 1 cut(s) 273
NlaIII CATG 1 cut(s) 613
NlaIV GGNNCC 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 152
PciSI GCTCTTC 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 232, 404, 550
Psp6I CCWGG 3 cut(s) 178, 289, 454
PspGI CCWGG 3 cut(s) 178, 289, 454
PspN4I GGNNCC 1 cut(s) 341
PspPI GGNCC 3 cut(s) 99, 458, 512
RsaI GTAC 3 cut(s) 436, 526, 541
RsaNI GTAC 3 cut(s) 435, 525, 540
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 3 cut(s) 219, 254, 616
SatI GCNGC 3 cut(s) 231, 403, 549
Sau3AI GATC 1 cut(s) 273
Sau96I GGNCC 3 cut(s) 99, 458, 512
ScrFI CCNGG 3 cut(s) 180, 291, 456
SfaNI GCATC 1 cut(s) 454
SinI GGWCC 2 cut(s) 99, 512
SmlI CTYRAG 5 cut(s) 406, 439, 469, 499, 529
SmoI CTYRAG 5 cut(s) 406, 439, 469, 499, 529
Sse9I AATT 7 cut(s) 42, 57, 70, 108, 140, 332, 582
SsiI CCGC 1 cut(s) 548
SspMI CTAG 2 cut(s) 240, 387
StyD4I CCNGG 3 cut(s) 178, 289, 454
TaaI ACNGT 4 cut(s) 103, 116, 361, 556
TaiI ACGT 1 cut(s) 195
TasI AATT 7 cut(s) 42, 57, 70, 108, 140, 332, 582
TauI GCSGC 1 cut(s) 551
Tru1I TTAA 3 cut(s) 219, 254, 616
Tru9I TTAA 3 cut(s) 219, 254, 616
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 2 cut(s) 230, 402
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 176, 295
VpaK11BI GGWCC 2 cut(s) 99, 512
XcmI CCANNNNNNNNNTGG 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 302
XspI CTAG 2 cut(s) 240, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.