Rh2AG562000

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011188
Physical Location & Seq
Reverse (-)
79147237 .. 79152633
5397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG562000.1

Sequence Viewer

Length: 1272 bp
ATGGAGCCAGCTGTAGTGTGTAATAACCAGAGCAACGGCGATGACATCATCCGGTGGTTTGAAGATGTATCGGAGAATGCAGAGTTGGTTCAAACGCAGACGCTGAGGCGGATACTTGAGCTGAATTGGGGGGTGGAGTATCTCAAGAAATGGTTGGGGGACGTCGAAATCGATGAGGTGGATGAATGTGCATTGGAGTCACTTTACACGTCTTTGGTACCGCTTGTTTCACATGCGGATTTAGAGCCTTACATTCAGAGAATTGCAAATGGGGACTCAGCTCCTCTACTCACCCAACAACCCATGACTACTCTCTCTTTGAGTTCCGGAACAACAGAAGGAAGACAGAAGTATGTACCGTTTACTCGCCATAGCGCACAGACTACTCTTCAAATTTTCAGACTGGCTGCAGCTTACAGATCAAGGGTTTATCCAACCAGGGAAGGAGGAAAGATCCTAGAATTCATATACAGCAGCAAACAGTTCAAAACAAGGGGAGGATTAACCGCCGGAACAGCTACAACTCACTACTATGCTAGTCAAGAATTCAAAATCAAACAGGAAACGACCAAGTCATTCACTTGCAGCCCAGAAGAAGTAATTTCAAGTGGAGACAATAAACAAGCTACATATTGTCATCTCCTTCTTGGTCTCTTCTTCTCTGATCATGTAGAGTTCATAACCTCGACGTTTGCCTACAGCATAGTCCAGTCCTTTGTCACATTTGAAGAGCTCTGGAGAGACTTATGCATTGACATTAGAGATGGCAGTCTGAGTAAAAGAATCAACGTACCAAAAGTGCGAAAATCAGTGTTGGACATCATCTCCCCAAACCCGAAATTGGCATCAAAAATCGAAGCTGCGTGCAAGGAACTGGAAGACTTGGATTGGTTTGGTCTGGTCCCTAAGCTTTGGCCAAATGCGAAGTATGTGTATTCCATAATGACAGGGTCAATGCAACCATACTTGAAAAAACTACGGCGCTATGCAGCTGATGTGCCATTGGTTAGTGCTGAGTATGGATCAACTGAGAGCTGGATTGGGGTGAATGTGGATCCTTGTTTGCCTCCAGAGGATGTCACTTTTGCAGTCGTGCCAACTTTTTCGTACTTCGAATTCATACCTCTGTATAGACACAAGCAAGATTGCAATTTAGCCGTTGATGACTTCATAGAGGATAAACCAGTTCCACTCTCACAAGTCAAGGTTGGACAGCAGTACGAGATCGTCCTCACCACCTTCACCGGTAAGTTAAGTTACCGTTCCAACTAA

Protein Analysis

423

Amino Acids

47.96

Weight (kDa)

5.29

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 17 - 335 1.3e-101 GH3 family N-terminal domain
GH3_M PF23571 363 - 421 6e-11 GH3 family central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03400 AT4G03400
fragaria_vesca FvH4_6g44990 FvH4_6g44990
malus_domestica MD17G1081000.v1.1
prunus_persica Prupe.3G233900_v2.0.a1
pyrus_communis pycom09g01640
rosa_chinensis RchiOBHm_Chr2g0162261
rosa_laevigata RLG00000021331
rosa_multiflora Rmu_sc0004020.1_g000022
rosa_roxburghii Rroxscaffold_2G00088210
rosa_rugosa Rorug02G0495700 Rorug02G0495800 Rorug02G0495900 Rorug02G0496000
rosa_samantha Rh2AG562000 Rh2BG575100 Rh2CG545300 Rh2DG585000
rosa_wichuraiana Rw2G046540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 165
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 217
AccIII TCCGGA 1 cut(s) 326
AciI CCGC 4 cut(s) 109, 221, 236, 507
AclWI GGATC 4 cut(s) 448, 1030, 1049, 1062
AcoI YGGCCR 1 cut(s) 914
AcsI RAATTY 4 cut(s) 393, 461, 545, 1115
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 5 cut(s) 219, 357, 792, 1109, 1220
AfiI CCNNNNNNNGG 1 cut(s) 841
AflIII ACRYGT 1 cut(s) 207
AgeI ACCGGT 1 cut(s) 1244
AgsI TTSAA 8 cut(s) 62, 92, 392, 487, 550, 606, 728, 970
AjiI CACGTC 1 cut(s) 210
AjnI CCWGG 1 cut(s) 437
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 3 cut(s) 606, 656, 735
AlwI GGATC 4 cut(s) 448, 1030, 1049, 1062
AlwNI CAGNNNCTG 1 cut(s) 103
Aor13HI TCCGGA 1 cut(s) 326
AoxI GGCC 1 cut(s) 914
ApeKI GCWGC 6 cut(s) 407, 410, 474, 585, 860, 989
ApoI RAATTY 4 cut(s) 393, 461, 545, 1115
AsiGI ACCGGT 1 cut(s) 1244
Asp718I GGTACC 1 cut(s) 217
AspLEI GCGC 2 cut(s) 377, 984
AspS9I GGNCC 1 cut(s) 901
AsuHPI GGTGA 4 cut(s) 283, 1057, 1225, 1234
AsuII TTCGAA 1 cut(s) 1113
AvaII GGWCC 1 cut(s) 901
BalI TGGCCA 1 cut(s) 916
BamHI GGATCC 1 cut(s) 1054
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 735
BbsI GAAGAC 2 cut(s) 349, 885
Bbv12I GWGCWC 1 cut(s) 735
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 6 cut(s) 394, 422, 486, 597, 847, 1001
BccI CCATC 1 cut(s) 758
BceAI ACGGC 3 cut(s) 52, 995, 1142
BciT130I CCWGG 1 cut(s) 439
BciVI GTATCC 1 cut(s) 105
BclI TGATCA 1 cut(s) 664
BcoDI GTCTC 3 cut(s) 606, 656, 735
BfaI CTAG 2 cut(s) 458, 537
BfmI CTRYAG 3 cut(s) 12, 408, 697
BfoI RGCGCY 1 cut(s) 985
BfuI GTATCC 1 cut(s) 105
BisI GCNGC 6 cut(s) 408, 411, 475, 586, 861, 990
BlsI GCNGC 6 cut(s) 409, 412, 476, 587, 862, 991
Bme1390I CCNGG 1 cut(s) 439
Bme18I GGWCC 1 cut(s) 901
BmgBI CACGTC 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 901
BmiI GGNNCC 4 cut(s) 6, 219, 903, 1056
BmrFI CCNGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 854
BpiI GAAGAC 2 cut(s) 349, 885
BpmI CTGGAG 2 cut(s) 757, 1053
Bpu10I CCTNAGC 2 cut(s) 104, 906
Bpu14I TTCGAA 1 cut(s) 1113
BpuEI CTTGAG 2 cut(s) 128, 137
Bsa29I ATCGAT 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 1 cut(s) 438
BsaWI WCCGGW 3 cut(s) 51, 326, 1244
BsaXI ACNNNNNCTCC 2 cut(s) 809, 839
Bsc4I CCNNNNNNNGG 1 cut(s) 841
Bse118I RCCGGY 1 cut(s) 1244
Bse1I ACTGG 4 cut(s) 408, 709, 879, 1184
BseAI TCCGGA 1 cut(s) 326
BseBI CCWGG 1 cut(s) 439
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 438
BseGI GGATG 3 cut(s) 48, 187, 1081
BseLI CCNNNNNNNGG 1 cut(s) 841
BseMII CTCAG 5 cut(s) 95, 291, 764, 1005, 1020
BseNI ACTGG 4 cut(s) 408, 709, 879, 1184
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 6 cut(s) 394, 422, 486, 597, 847, 1001
BshFI GGCC 1 cut(s) 916
BshNI GGYRCC 1 cut(s) 217
BshTI ACCGGT 1 cut(s) 1244
BshVI ATCGAT 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 735
BsiSI CCGG 4 cut(s) 52, 327, 510, 1245
BslFI GGGAC 3 cut(s) 173, 287, 887
BslI CCNNNNNNNGG 1 cut(s) 841
BsmAI GTCTC 3 cut(s) 606, 656, 735
BsmFI GGGAC 3 cut(s) 173, 287, 887
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 1 cut(s) 916
Bso31I GGTCTC 1 cut(s) 656
Bsp119I TTCGAA 1 cut(s) 1113
Bsp1286I GDGCHC 1 cut(s) 735
Bsp13I TCCGGA 1 cut(s) 326
Bsp143I GATC 6 cut(s) 419, 453, 664, 1022, 1054, 1224
BspACI CCGC 4 cut(s) 109, 221, 236, 507
BspANI GGCC 1 cut(s) 916
BspCNI CTCAG 5 cut(s) 96, 290, 765, 1006, 1021
BspDI ATCGAT 1 cut(s) 171
BspEI TCCGGA 1 cut(s) 326
BspLI GGNNCC 4 cut(s) 6, 219, 903, 1056
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 4 cut(s) 448, 1030, 1049, 1062
BspQI GCTCTTC 1 cut(s) 723
BspT104I TTCGAA 1 cut(s) 1113
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 656
BsrFI RCCGGY 1 cut(s) 1244
BsrI ACTGG 4 cut(s) 408, 709, 879, 1184
BssAI RCCGGY 1 cut(s) 1244
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 6 cut(s) 419, 453, 664, 1022, 1054, 1224
BssNI GRCGYC 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 3 cut(s) 360, 483, 1262
Bst6I CTCTTC 3 cut(s) 393, 659, 723
BstACI GRCGYC 1 cut(s) 162
BstBI TTCGAA 1 cut(s) 1113
BstC8I GCNNGC 2 cut(s) 9, 865
BstDEI CTNAG 6 cut(s) 104, 277, 773, 906, 1014, 1029
BstF5I GGATG 3 cut(s) 48, 187, 1081
BstH2I RGCGCY 1 cut(s) 985
BstHHI GCGC 2 cut(s) 377, 984
BstKTI GATC 6 cut(s) 422, 456, 667, 1025, 1057, 1227
BstMAI GTCTC 3 cut(s) 606, 656, 735
BstMBI GATC 6 cut(s) 419, 453, 664, 1022, 1054, 1224
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstNI CCWGG 1 cut(s) 439
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 437
BstSFI CTRYAG 3 cut(s) 12, 408, 697
BstV1I GCAGC 6 cut(s) 394, 422, 486, 597, 847, 1001
BstV2I GAAGAC 2 cut(s) 349, 885
BstX2I RGATCY 2 cut(s) 453, 1054
BstYI RGATCY 2 cut(s) 453, 1054
Bsu15I ATCGAT 1 cut(s) 171
BsuI GTATCC 1 cut(s) 105
BsuRI GGCC 1 cut(s) 916
BsuTUI ATCGAT 1 cut(s) 171
BtgZI GCGATG 1 cut(s) 54
BtrI CACGTC 1 cut(s) 210
BtsCI GGATG 3 cut(s) 48, 187, 1081
BtsIMutI CAGTG 1 cut(s) 816
Cac8I GCNNGC 2 cut(s) 9, 865
CaiI CAGNNNCTG 1 cut(s) 103
CfoI GCGC 2 cut(s) 377, 984
Cfr10I RCCGGY 1 cut(s) 1244
Cfr13I GGNCC 1 cut(s) 901
ClaI ATCGAT 1 cut(s) 171
CseI GACGC 1 cut(s) 109
Csp6I GTAC 5 cut(s) 218, 356, 791, 1108, 1219
CspAI ACCGGT 1 cut(s) 1244
CviAII CATG 3 cut(s) 233, 304, 668
CviQI GTAC 5 cut(s) 218, 356, 791, 1108, 1219
DdeI CTNAG 6 cut(s) 104, 277, 773, 906, 1014, 1029
DpnI GATC 6 cut(s) 421, 455, 666, 1024, 1056, 1226
DpnII GATC 6 cut(s) 419, 453, 664, 1022, 1054, 1224
EaeI YGGCCR 1 cut(s) 914
Eam1104I CTCTTC 3 cut(s) 393, 659, 723
EarI CTCTTC 3 cut(s) 393, 659, 723
EciI GGCGGA 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 735
Eco31I GGTCTC 1 cut(s) 656
Eco47I GGWCC 1 cut(s) 901
Eco53kI GAGCTC 1 cut(s) 733
EcoICRI GAGCTC 1 cut(s) 733
EcoRI GAATTC 3 cut(s) 461, 545, 1115
EcoRII CCWGG 1 cut(s) 437
EcoT22I ATGCAT 1 cut(s) 752
EcoT38I GRGCYC 1 cut(s) 735
FaeI CATG 3 cut(s) 236, 307, 671
FaqI GGGAC 3 cut(s) 173, 287, 887
FatI CATG 3 cut(s) 232, 303, 667
FbaI TGATCA 1 cut(s) 664
Fnu4HI GCNGC 6 cut(s) 408, 411, 475, 586, 861, 990
FokI GGATG 3 cut(s) 35, 194, 1088
FriOI GRGCYC 1 cut(s) 735
Fsp4HI GCNGC 6 cut(s) 408, 411, 475, 586, 861, 990
FspBI CTAG 2 cut(s) 458, 537
GlaI GCGC 2 cut(s) 376, 983
GluI GCNGC 6 cut(s) 408, 411, 475, 586, 861, 990
GsuI CTGGAG 2 cut(s) 757, 1053
HaeII RGCGCY 1 cut(s) 985
HaeIII GGCC 1 cut(s) 916
HapII CCGG 4 cut(s) 52, 327, 510, 1245
HgaI GACGC 1 cut(s) 109
HhaI GCGC 2 cut(s) 377, 984
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 3 cut(s) 236, 307, 671
Hin6I GCGC 2 cut(s) 375, 982
HinP1I GCGC 2 cut(s) 375, 982
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 3 cut(s) 197, 275, 783
HpaII CCGG 4 cut(s) 52, 327, 510, 1245
HphI GGTGA 4 cut(s) 283, 1057, 1225, 1234
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 5 cut(s) 73, 258, 401, 664, 774
Hpy188III TCNNGA 5 cut(s) 145, 327, 542, 736, 1070
Hpy8I GTNNAC 1 cut(s) 363
Hpy99I CGWCG 2 cut(s) 167, 691
HpyAV CCTTC 4 cut(s) 332, 437, 653, 1249
HpyCH4III ACNGT 3 cut(s) 360, 483, 1262
HpyCH4IV ACGT 4 cut(s) 162, 209, 689, 789
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpyF3I CTNAG 6 cut(s) 104, 277, 773, 906, 1014, 1029
HpySE526I ACGT 4 cut(s) 162, 209, 689, 789
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 3 cut(s) 236, 307, 671
HspAI GCGC 2 cut(s) 375, 982
Kpn2I TCCGGA 1 cut(s) 326
KpnI GGTACC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 6 cut(s) 419, 453, 664, 1022, 1054, 1224
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 2 cut(s) 4, 286
Lsp1109I GCAGC 6 cut(s) 394, 422, 486, 597, 847, 1001
LweI GCATC 1 cut(s) 854
MaeI CTAG 2 cut(s) 458, 537
MaeII ACGT 4 cut(s) 162, 209, 689, 789
MaeIII GTNAC 4 cut(s) 198, 718, 1078, 1256
MalI GATC 6 cut(s) 421, 455, 666, 1024, 1056, 1226
MboI GATC 6 cut(s) 419, 453, 664, 1022, 1054, 1224
MboII GAAGA 8 cut(s) 74, 354, 380, 605, 646, 649, 740, 890
MflI RGATCY 2 cut(s) 453, 1054
MhlI GDGCHC 1 cut(s) 735
MlsI TGGCCA 1 cut(s) 916
MluCI AATT 9 cut(s) 124, 261, 393, 461, 545, 600, 839, 1115, 1150
MluNI TGGCCA 1 cut(s) 916
MlyI GAGTC 2 cut(s) 206, 269
MmeI TCCRAC 3 cut(s) 458, 795, 1189
Mox20I TGGCCA 1 cut(s) 916
Mph1103I ATGCAT 1 cut(s) 752
MroI TCCGGA 1 cut(s) 326
MscI TGGCCA 1 cut(s) 916
MseI TTAA 2 cut(s) 503, 1253
MslI CAYNNNNRTG 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 916
MspA1I CMGCKG 2 cut(s) 11, 992
MspI CCGG 4 cut(s) 52, 327, 510, 1245
MspR9I CCNGG 1 cut(s) 439
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 515
NdeII GATC 6 cut(s) 419, 453, 664, 1022, 1054, 1224
NlaIII CATG 3 cut(s) 236, 307, 671
NlaIV GGNNCC 4 cut(s) 6, 219, 903, 1056
NmuCI GTSAC 3 cut(s) 198, 718, 1078
NsiI ATGCAT 1 cut(s) 752
NspI RCATGY 1 cut(s) 236
NspV TTCGAA 1 cut(s) 1113
PciSI GCTCTTC 1 cut(s) 723
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 1 cut(s) 82
PfeI GAWTC 1 cut(s) 783
PflFI GACNNNGTC 2 cut(s) 571, 949
PinAI ACCGGT 1 cut(s) 1244
PkrI GCNGC 6 cut(s) 409, 412, 476, 587, 862, 991
PleI GAGTC 2 cut(s) 205, 269
PpsI GAGTC 2 cut(s) 205, 269
Psp124BI GAGCTC 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 437
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 4 cut(s) 6, 219, 903, 1056
PspPI GGNCC 1 cut(s) 901
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 103
PsuI RGATCY 2 cut(s) 453, 1054
PsyI GACNNNGTC 2 cut(s) 571, 949
PvuII CAGCTG 2 cut(s) 11, 992
RsaI GTAC 5 cut(s) 219, 357, 792, 1109, 1220
RsaNI GTAC 5 cut(s) 218, 356, 791, 1108, 1219
RseI CAYNNNNRTG 1 cut(s) 531
SacI GAGCTC 1 cut(s) 735
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 2 cut(s) 503, 1253
SatI GCNGC 6 cut(s) 408, 411, 475, 586, 861, 990
Sau3AI GATC 6 cut(s) 419, 453, 664, 1022, 1054, 1224
Sau96I GGNCC 1 cut(s) 901
SchI GAGTC 2 cut(s) 206, 269
ScrFI CCNGG 1 cut(s) 439
SduI GDGCHC 1 cut(s) 735
SfaNI GCATC 1 cut(s) 854
SfcI CTRYAG 3 cut(s) 12, 408, 697
SfuI TTCGAA 1 cut(s) 1113
SinI GGWCC 1 cut(s) 901
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 2 cut(s) 116, 143
SmoI CTYRAG 2 cut(s) 116, 143
Sse9I AATT 9 cut(s) 124, 261, 393, 461, 545, 600, 839, 1115, 1150
SsiI CCGC 4 cut(s) 109, 221, 236, 507
SspMI CTAG 2 cut(s) 458, 537
SstI GAGCTC 1 cut(s) 735
StyD4I CCNGG 1 cut(s) 437
TaaI ACNGT 3 cut(s) 360, 483, 1262
TaiI ACGT 4 cut(s) 165, 212, 692, 792
TaqI TCGA 5 cut(s) 165, 171, 686, 855, 1113
TasI AATT 9 cut(s) 124, 261, 393, 461, 545, 600, 839, 1115, 1150
TfiI GAWTC 1 cut(s) 783
Tru1I TTAA 2 cut(s) 503, 1253
Tru9I TTAA 2 cut(s) 503, 1253
TscAI CASTG 1 cut(s) 816
TseFI GTSAC 3 cut(s) 198, 718, 1078
TseI GCWGC 6 cut(s) 407, 410, 474, 585, 860, 989
Tsp45I GTSAC 3 cut(s) 198, 718, 1078
TspDTI ATGAA 5 cut(s) 198, 454, 667, 1108, 1159
TspRI CASTG 1 cut(s) 816
Tth111I GACNNNGTC 2 cut(s) 571, 949
VpaK11BI GGWCC 1 cut(s) 901
XapI RAATTY 4 cut(s) 393, 461, 545, 1115
XceI RCATGY 1 cut(s) 236
XspI CTAG 2 cut(s) 458, 537
ZraI GACGTC 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.