Rorug02G0518300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014143
Physical Location & Seq
Forward (+)
64969928 .. 64974547
4620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0518300.1

Sequence Viewer

Length: 612 bp
ATGAAACTGGAGCTCGATCACGTTACCGACGAGATGTGCCGCACAATCGAAGTCGACGGAGGCGTCCACCCCTCTCCGGCCGCGGCCGCCGCGTCTGACGGAGAGTGCGCCGATGATCTCTTCATTCCTCCGCTCAACTTCGCCATGGTCGATAACGGCATTTTCCGGTCCGGCTTCCCTGACTCCAACAACTTCTCCTTCCTCCAAACCCTAGGCCTCCGCTCCATTATATGTCTGTGCCCGGAGCCGTATCCAGAGACGAACGTGGAGTTCCTCAAGTCGAACGGGATCAAGCTCTTTCAATTTGGGATCGAAGGCTATAAGGAGCCTTTTATAAACATCCCTGAGGATACGATCCGTGAAGCACTAAAAGTTGTCCTTGATGTAAGGAATCACCCCATCTTAATTCATTGCAAACGAGGGAAGCATCGAACTGGTTGTGTGGTGGGATGCTTGAGAAAACTGCAGAGATGGTGCCTGTCATCCGTTTTTGATGAGTACCAGCGTTTTGCAGCCGCCAAAGCCAGAGTTGCTGATCAAAGGTTTATGGAGATGTTTGATGTTTCTAGTTTGAAACATTTACAGATGCCATTTTCATGTTCGAAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spo12

Protein Analysis

203

Amino Acids

22.93

Weight (kDa)

6.3

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Y_phosphatase2 PF03162 42 - 194 1.7e-64 Tyrosine phosphatase family
PTP-SAK PF22784 65 - 153 1.2e-06 Swiss Army Knife protein, DSP-PTPase phosphatase domain
PTP-NADK PF22741 92 - 178 3.4e-07 DSP-PTPase phosphatase fused to NAD+ Kinase
Y_phosphatase3 PF13350 115 - 172 1.5e-06 Tyrosine phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02120
fragaria_vesca FvH4_6g46850
malus_domestica MD09G1066800.v1.1 MD17G1059700.v1.1
prunus_persica Prupe.3G254900_v2.0.a1
pyrus_communis pycom111g05610 pycom17g05940
rosa_chinensis RchiOBHm_Chr2g0165841
rosa_laevigata RLG00000021561
rosa_multiflora Rmu_sc0008709.1_g000002
rosa_roxburghii Rroxscaffold_2G00085450
rosa_rugosa Rorug02G0518300
rosa_samantha Rh2AG583900 Rh2BG596000 Rh2CG566800 Rh2DG605800
rosa_wichuraiana Rw2G048700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 335
AasI GACNNNNNNGTC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 474
AccBSI CCGCTC 2 cut(s) 133, 222
AccI GTMKAC 1 cut(s) 54
AccII CGCG 2 cut(s) 83, 92
AciI CCGC 8 cut(s) 40, 81, 83, 87, 90, 131, 220, 516
AclWI GGATC 3 cut(s) 296, 317, 349
AcoI YGGCCR 2 cut(s) 78, 84
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 1 cut(s) 500
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 2 cut(s) 302, 574
AluBI AGCT 2 cut(s) 13, 295
AluI AGCT 2 cut(s) 13, 295
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 3 cut(s) 296, 317, 349
AoxI GGCC 3 cut(s) 78, 84, 214
ApeKI GCWGC 1 cut(s) 512
AspA2I CCTAGG 1 cut(s) 211
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 168
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 386
AsuII TTCGAA 1 cut(s) 602
AvaII GGWCC 1 cut(s) 168
AvrII CCTAGG 1 cut(s) 211
AxyI CCTNAGG 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 242
BanI GGYRCC 1 cut(s) 474
BanII GRGCYC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 1 cut(s) 524
BccI CCATC 2 cut(s) 407, 465
BceAI ACGGC 2 cut(s) 172, 232
BciVI GTATCC 2 cut(s) 261, 343
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 251
BfaI CTAG 2 cut(s) 212, 567
BfmI CTRYAG 1 cut(s) 464
BfuI GTATCC 2 cut(s) 261, 343
BisI GCNGC 7 cut(s) 40, 81, 84, 87, 90, 513, 516
BlnI CCTAGG 1 cut(s) 211
BlsI GCNGC 7 cut(s) 41, 82, 85, 88, 91, 514, 517
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 1 cut(s) 168
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 3 cut(s) 246, 327, 476
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 3 cut(s) 436, 440, 576
BpmI CTGGAG 1 cut(s) 29
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 2 cut(s) 260, 475
BpuMI CCSGG 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 81, 144, 211
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 12, 439
Bse21I CCTNAGG 1 cut(s) 345
Bse3DI GCAATG 1 cut(s) 409
BseDI CCNNGG 3 cut(s) 81, 144, 211
BseGI GGATG 3 cut(s) 339, 455, 482
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 2 cut(s) 12, 439
BseSI GKGCMC 1 cut(s) 242
BseX3I CGGCCG 2 cut(s) 78, 84
BseXI GCAGC 1 cut(s) 524
Bsh1236I CGCG 2 cut(s) 83, 92
Bsh1285I CGRYCG 2 cut(s) 81, 87
BshFI GGCC 3 cut(s) 80, 86, 216
BshNI GGYRCC 1 cut(s) 474
BsiEI CGRYCG 2 cut(s) 81, 87
BsiHKAI GWGCWC 1 cut(s) 15
BsiSI CCGG 4 cut(s) 77, 166, 171, 242
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 251
BsmBI CGTCTC 1 cut(s) 251
BsnI GGCC 3 cut(s) 80, 86, 216
Bsp119I TTCGAA 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 15, 242
Bsp143I GATC 6 cut(s) 16, 115, 288, 309, 354, 535
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 8 cut(s) 40, 81, 83, 87, 90, 131, 220, 516
BspANI GGCC 3 cut(s) 80, 86, 216
BspCNI CTCAG 1 cut(s) 337
BspFNI CGCG 2 cut(s) 83, 92
BspLI GGNNCC 3 cut(s) 246, 327, 476
BspMAI CTGCAG 1 cut(s) 468
BspPI GGATC 3 cut(s) 296, 317, 349
BspT104I TTCGAA 1 cut(s) 602
BspT107I GGYRCC 1 cut(s) 474
BsrBI CCGCTC 2 cut(s) 133, 222
BsrDI GCAATG 1 cut(s) 409
BsrI ACTGG 2 cut(s) 12, 439
BssECI CCNNGG 3 cut(s) 81, 144, 211
BssMI GATC 6 cut(s) 16, 115, 288, 309, 354, 535
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 2 cut(s) 144, 211
Bst6I CTCTTC 2 cut(s) 125, 599
BstACI GRCGYC 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 602
BstDEI CTNAG 1 cut(s) 345
BstDSI CCRYGG 2 cut(s) 81, 144
BstF5I GGATG 3 cut(s) 339, 455, 482
BstFNI CGCG 2 cut(s) 83, 92
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 6 cut(s) 19, 118, 291, 312, 357, 538
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 6 cut(s) 16, 115, 288, 309, 354, 535
BstMCI CGRYCG 2 cut(s) 81, 87
BstMWI GCNNNNNNNGC 4 cut(s) 86, 89, 521, 530
BstSCI CCNGG 1 cut(s) 240
BstSFI CTRYAG 1 cut(s) 464
BstSLI GKGCMC 1 cut(s) 242
BstUI CGCG 2 cut(s) 83, 92
BstV1I GCAGC 1 cut(s) 524
BstZI CGGCCG 2 cut(s) 78, 84
Bsu36I CCTNAGG 1 cut(s) 345
BsuI GTATCC 2 cut(s) 261, 343
BsuRI GGCC 3 cut(s) 80, 86, 216
BtgI CCRYGG 2 cut(s) 81, 144
BtsCI GGATG 3 cut(s) 339, 455, 482
CciNI GCGGCCGC 1 cut(s) 84
CfoI GCGC 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 168
Cfr42I CCGCGG 1 cut(s) 84
CpoI CGGWCCG 1 cut(s) 168
CseI GACGC 2 cut(s) 52, 81
Csp6I GTAC 1 cut(s) 499
CspI CGGWCCG 1 cut(s) 168
CviAII CATG 2 cut(s) 145, 597
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 1 cut(s) 345
DpnI GATC 6 cut(s) 18, 117, 290, 311, 356, 537
DpnII GATC 6 cut(s) 16, 115, 288, 309, 354, 535
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 2 cut(s) 78, 84
EagI CGGCCG 2 cut(s) 78, 84
Eam1104I CTCTTC 2 cut(s) 125, 599
EarI CTCTTC 2 cut(s) 125, 599
Ecl136II GAGCTC 1 cut(s) 13
EclXI CGGCCG 2 cut(s) 78, 84
Eco130I CCWWGG 2 cut(s) 144, 211
Eco147I AGGCCT 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 168
Eco52I CGGCCG 2 cut(s) 78, 84
Eco53kI GAGCTC 1 cut(s) 13
Eco81I CCTNAGG 1 cut(s) 345
EcoICRI GAGCTC 1 cut(s) 13
EcoT14I CCWWGG 2 cut(s) 144, 211
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 2 cut(s) 144, 211
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 2 cut(s) 148, 600
FaiI YATR 7 cut(s) 146, 230, 232, 321, 335, 548, 598
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FatI CATG 2 cut(s) 144, 596
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 7 cut(s) 40, 81, 84, 87, 90, 513, 516
FokI GGATG 3 cut(s) 326, 462, 469
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 7 cut(s) 40, 81, 84, 87, 90, 513, 516
FspBI CTAG 2 cut(s) 212, 567
GlaI GCGC 1 cut(s) 109
GluI GCNGC 7 cut(s) 40, 81, 84, 87, 90, 513, 516
GsuI CTGGAG 1 cut(s) 29
HaeIII GGCC 3 cut(s) 80, 86, 216
HapII CCGG 4 cut(s) 77, 166, 171, 242
HgaI GACGC 2 cut(s) 52, 81
HhaI GCGC 1 cut(s) 110
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 2 cut(s) 148, 600
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 2 cut(s) 182, 391
HpaII CCGG 4 cut(s) 77, 166, 171, 242
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 2 cut(s) 55, 67
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 2 cut(s) 55, 67
Hpy99I CGWCG 2 cut(s) 32, 59
HpyAV CCTTC 2 cut(s) 208, 308
HpyCH4IV ACGT 2 cut(s) 21, 264
HpyCH4V TGCA 3 cut(s) 414, 466, 512
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 89, 521, 530
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 2 cut(s) 21, 264
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 2 cut(s) 148, 600
HspAI GCGC 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 535
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 6 cut(s) 16, 115, 288, 309, 354, 535
LmnI GCTCC 4 cut(s) 10, 227, 244, 325
Lsp1109I GCAGC 1 cut(s) 524
LweI GCATC 3 cut(s) 436, 440, 576
MaeI CTAG 2 cut(s) 212, 567
MaeII ACGT 2 cut(s) 21, 264
MaeIII GTNAC 1 cut(s) 22
MalI GATC 6 cut(s) 18, 117, 290, 311, 356, 537
MbiI CCGCTC 2 cut(s) 133, 222
MboI GATC 6 cut(s) 16, 115, 288, 309, 354, 535
MboII GAAGA 1 cut(s) 112
MhlI GDGCHC 2 cut(s) 15, 242
MluCI AATT 2 cut(s) 302, 405
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 9 cut(s) 53, 82, 138, 212, 227, 284, 340, 413, 600
MseI TTAA 1 cut(s) 404
MslI CAYNNNNRTG 1 cut(s) 595
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 4 cut(s) 77, 166, 171, 242
MspR9I CCNGG 1 cut(s) 242
MvnI CGCG 2 cut(s) 83, 92
MwoI GCNNNNNNNGC 4 cut(s) 86, 89, 521, 530
NciI CCSGG 1 cut(s) 242
NcoI CCATGG 1 cut(s) 144
NdeII GATC 6 cut(s) 16, 115, 288, 309, 354, 535
NlaIII CATG 2 cut(s) 148, 600
NlaIV GGNNCC 3 cut(s) 246, 327, 476
NotI GCGGCCGC 1 cut(s) 84
NspV TTCGAA 1 cut(s) 602
PceI AGGCCT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 60, 147
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 7 cut(s) 41, 82, 85, 88, 91, 514, 517
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PsiI TTATAA 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 15
PspN4I GGNNCC 3 cut(s) 246, 327, 476
PspPI GGNCC 1 cut(s) 168
PstI CTGCAG 1 cut(s) 468
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 1 cut(s) 595
Rsr2I CGGWCCG 1 cut(s) 168
RsrII CGGWCCG 1 cut(s) 168
SacI GAGCTC 1 cut(s) 15
SacII CCGCGG 1 cut(s) 84
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 7 cut(s) 40, 81, 84, 87, 90, 513, 516
Sau3AI GATC 6 cut(s) 16, 115, 288, 309, 354, 535
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 2 cut(s) 15, 242
SetI ASST 6 cut(s) 15, 24, 267, 297, 545, 611
SfaNI GCATC 3 cut(s) 436, 440, 576
SfcI CTRYAG 1 cut(s) 464
Sfr303I CCGCGG 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 602
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 595
SmlI CTYRAG 2 cut(s) 275, 454
SmoI CTYRAG 2 cut(s) 275, 454
Sse9I AATT 2 cut(s) 302, 405
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 8 cut(s) 40, 81, 83, 87, 90, 131, 220, 516
SspMI CTAG 2 cut(s) 212, 567
SstI GAGCTC 1 cut(s) 15
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 2 cut(s) 144, 211
TaiI ACGT 2 cut(s) 24, 267
TaqI TCGA 8 cut(s) 15, 48, 54, 150, 281, 312, 430, 602
TasI AATT 2 cut(s) 302, 405
TauI GCSGC 6 cut(s) 42, 83, 86, 89, 92, 518
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TseI GCWGC 1 cut(s) 512
TspDTI ATGAA 4 cut(s) 17, 112, 398, 585
TspGWI ACGGA 4 cut(s) 72, 114, 347, 475
VpaK11BI GGWCC 1 cut(s) 168
XmaJI CCTAGG 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 2 cut(s) 212, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.