Rorug03G0073500

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008973
Physical Location & Seq
Forward (+)
5808829 .. 5814070
5242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0073500.1

Sequence Viewer

Length: 1737 bp
ATGGGATTGGAGGAAAGGGCTGCCACATATAACAGCCAGAACGGCAAATGTAATCAGACATTCACTATTTGCAAGCATGGATTTACTGATCTATCGCACATTTCTCCATTGGTGTTCATATACCTCTTGAAAGAATGTTACTTAATTGGCACATGCAAGGCAACTAAGAAGTTCCGAGCTCTTCAAGAACAGGTCCATCTAGTGCTACGCAATGATCCCCAACCTGGACCAGCTACTTTTGTTATTCAGTGCCTCTATGTCTTGCCTATATTTGACTTATACTCTGAAGGGTTTAGTCATTTAATTATATCTGCTCTTCGTCGCTTCCTAAAAGAAGAAACTTCCGCGGGGGACTCGTTGGAAGCAAAAGACCTTGCTGCCCAGTTATTTCTAGATATTGTTGGGGGCTCTGTCAACCATGATGAGAGGATAGTTGAGAAGATGCTTGAAGTTTTTGATGTTCAGTTAGAGAATATTGAAAAAGTTTTGTGCCCAAATGCAATAAATGATGACACTTTTAGCATGGCAACAAAATTTGTTGAGGATTATATATATCAGTTGATAGAGTCTCAAACATACATGACTGCGGTTACTCTATTGGCGCACTTCTCTATCCGCCAGTCTGGTAAATCTTTTCTGCTTTGTATGATAGAAAGCCAACAATTGAAAGCAGCAGAGAAGTGGGCAACATTCATCGGAAAGCCAATGTTATGTGTTCTGGTCCAAGAGTATGTTGATAGAAACATGCTAAAGAATGCTTACGAGATTATACACAAAAACAATCTCCGACAGGAATTCCCCGACGTTCATCACAAGTATAAAGAGAGCTCATTAAAGAAACTAGCAGAAAAAGGATGCTGGGATGTTGCAGAAGCAAAGACAAAGAGTAATAGACAACTCCTTGAATACCTGGTTTATTTAGCCATGGAAGCTGGTTACTCAGAAAAGGTTGATGAACTATGTGATCGATATTCACTTAAAGGTTTCTTGAATGCCAAAGTGCCTGAGCCAAGCCTTCTCCATAGTCGCTATTTGCATCTGGATGAGTTAGTTGTAGAAGGTGTCATTTGGGTTGATGAAGTTAATGCTTTGCGTAAGGCAACCTGTCATCTTGAGGAATGCAAAGTTGTTGGTCTTGATTGTGAATGGAAACCGAATTATGTAAAAGGTTGCAAACCAAACAAGGTTTCTATTATGCAAATTGCTTCTGATAAGATGGTTTTCATACTTGACTTGATCAAATTATATGAAGACGTGCCTGATGTTCTGGATGACAGCTTAACTCGTATTCTACAGTCTCCTAGGATCCTAAAACTTGGCTATAATTTTCAATGTGATATGAGGCAGCTGGCTCAGTCATATGAAGAGTTAGAGTGTTTCAAGCACTATCAGATGCTACTGGACATTCAGAATGTGTTCAAAGAACCTCGGGGTGGTCTATCCGGGCTTGCAAAGAAAATACTTGGAGTTGGTTTGAACAAGACAAGAAGGAACAGCAATTGGGAGCAACGGCCTCTAACACCCAATCAGCTGGAATATGCAGCTCTCGATGCAGCTGTACTGGTTCACATATTTGGCCACGTTCGCGGTCATTCTCAGCCTGCTGATGGAGAGGGACATGGAAAACTCGAGTGGAAATCCTGCATTGTTTCCGACATGAATAACATCAAGAAGTCCAAGAAGGGAAGTCGAAAGGAGTCTTCTGAAGCTCAGACAAAGGAGCATTTAGCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

578

Amino Acids

65.95

Weight (kDa)

6.43

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 356 - 527 4.6e-22 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 347, 1587
AciI CCGC 5 cut(s) 345, 347, 587, 616, 1587
AclWI GGATC 3 cut(s) 209, 1300, 1313
AcoI YGGCCR 1 cut(s) 1576
AcsI RAATTY 2 cut(s) 533, 794
AcuI CTGAAG 2 cut(s) 306, 1725
AfaI GTAC 1 cut(s) 1560
AfiI CCNNNNNNNGG 3 cut(s) 224, 1433, 1607
AjiI CACGTC 1 cut(s) 1255
AjnI CCWGG 2 cut(s) 223, 909
AjuI GAANNNNNNNTTGG 2 cut(s) 1171, 1203
Alw21I GWGCWC 2 cut(s) 181, 830
Alw26I GTCTC 2 cut(s) 573, 1302
AlwI GGATC 3 cut(s) 209, 1300, 1313
Ama87I CYCGRG 2 cut(s) 1428, 1628
AoxI GGCC 2 cut(s) 1511, 1576
ApeKI GCWGC 6 cut(s) 20, 377, 671, 1345, 1541, 1553
ApoI RAATTY 2 cut(s) 533, 794
Asp700I GAANNNNTTC 1 cut(s) 1415
AspA2I CCTAGG 1 cut(s) 1301
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 3 cut(s) 193, 227, 721
AsuC2I CCSGG 1 cut(s) 1444
AvaI CYCGRG 2 cut(s) 1428, 1628
AvaII GGWCC 3 cut(s) 193, 227, 721
AvrII CCTAGG 1 cut(s) 1301
BaeGI GKGCMC 1 cut(s) 494
BalI TGGCCA 1 cut(s) 1578
BamHI GGATCC 1 cut(s) 1305
BanII GRGCYC 3 cut(s) 181, 410, 830
BbsI GAAGAC 2 cut(s) 1257, 1692
Bbv12I GWGCWC 2 cut(s) 181, 830
BbvI GCAGC 6 cut(s) 7, 364, 683, 1357, 1553, 1565
BccI CCATC 3 cut(s) 204, 1210, 1601
BceAI ACGGC 2 cut(s) 58, 1526
BciT130I CCWGG 2 cut(s) 225, 911
BclI TGATCA 1 cut(s) 1236
BcnI CCSGG 1 cut(s) 1444
BcoDI GTCTC 2 cut(s) 573, 1302
BfaI CTAG 4 cut(s) 200, 392, 842, 1302
BfmI CTRYAG 1 cut(s) 1292
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 6 cut(s) 21, 378, 672, 1346, 1542, 1554
BlnI CCTAGG 1 cut(s) 1301
BlsI GCNGC 6 cut(s) 22, 379, 673, 1347, 1543, 1555
Bme1390I CCNGG 3 cut(s) 225, 911, 1444
Bme18I GGWCC 3 cut(s) 193, 227, 721
BmeT110I CYCGRG 2 cut(s) 1428, 1628
BmgBI CACGTC 1 cut(s) 1255
BmgT120I GGNCC 3 cut(s) 193, 227, 721
BmiI GGNNCC 1 cut(s) 1307
BmrFI CCNGG 3 cut(s) 225, 911, 1444
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 5 cut(s) 432, 845, 1045, 1383, 1540
BmuI ACTGGG 1 cut(s) 376
BpiI GAAGAC 2 cut(s) 1257, 1692
Bpu10I CCTNAGC 1 cut(s) 1005
BpuEI CTTGAG 1 cut(s) 1133
BpuMI CCSGG 1 cut(s) 1444
Bsa29I ATCGAT 1 cut(s) 967
BsaJI CCNNGG 4 cut(s) 345, 924, 1301, 1427
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 1433, 1607
Bse1I ACTGG 4 cut(s) 382, 619, 1404, 1566
Bse3DI GCAATG 1 cut(s) 217
BseBI CCWGG 2 cut(s) 225, 911
BseCI ATCGAT 1 cut(s) 967
BseDI CCNNGG 4 cut(s) 345, 924, 1301, 1427
BseGI GGATG 4 cut(s) 860, 868, 1048, 1276
BseLI CCNNNNNNNGG 3 cut(s) 224, 1433, 1607
BseMI GCAATG 1 cut(s) 217
BseMII CTCAG 5 cut(s) 954, 996, 1367, 1610, 1724
BseNI ACTGG 4 cut(s) 382, 619, 1404, 1566
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 6 cut(s) 7, 364, 683, 1357, 1553, 1565
BseYI CCCAGC 1 cut(s) 858
Bsh1236I CGCG 2 cut(s) 347, 1587
BshFI GGCC 2 cut(s) 1513, 1578
BshVI ATCGAT 1 cut(s) 967
BsiHKAI GWGCWC 2 cut(s) 181, 830
BsiHKCI CYCGRG 2 cut(s) 1428, 1628
BsiSI CCGG 1 cut(s) 1443
BslFI GGGAC 2 cut(s) 365, 1629
BslI CCNNNNNNNGG 3 cut(s) 224, 1433, 1607
BsmAI GTCTC 2 cut(s) 573, 1302
BsmFI GGGAC 2 cut(s) 365, 1629
BsmI GAATGC 3 cut(s) 760, 997, 1124
BsnI GGCC 2 cut(s) 1513, 1578
BsoBI CYCGRG 2 cut(s) 1428, 1628
Bsp1286I GDGCHC 4 cut(s) 181, 410, 494, 830
Bsp143I GATC 5 cut(s) 88, 214, 964, 1236, 1305
Bsp19I CCATGG 1 cut(s) 924
BspACI CCGC 5 cut(s) 345, 347, 587, 616, 1587
BspANI GGCC 2 cut(s) 1513, 1578
BspCNI CTCAG 5 cut(s) 953, 997, 1366, 1609, 1723
BspDI ATCGAT 1 cut(s) 967
BspFNI CGCG 2 cut(s) 347, 1587
BspLI GGNNCC 1 cut(s) 1307
BspPI GGATC 3 cut(s) 209, 1300, 1313
BspQI GCTCTTC 2 cut(s) 186, 321
BsrDI GCAATG 1 cut(s) 217
BsrI ACTGG 4 cut(s) 382, 619, 1404, 1566
BssECI CCNNGG 4 cut(s) 345, 924, 1301, 1427
BssMI GATC 5 cut(s) 88, 214, 964, 1236, 1305
BssT1I CCWWGG 2 cut(s) 924, 1301
Bst2UI CCWGG 2 cut(s) 225, 911
Bst4CI ACNGT 1 cut(s) 1296
Bst6I CTCTTC 3 cut(s) 186, 321, 1359
BstC8I GCNNGC 4 cut(s) 74, 1350, 1449, 1602
BstDEI CTNAG 6 cut(s) 165, 940, 1005, 1353, 1596, 1710
BstDSI CCRYGG 2 cut(s) 345, 924
BstF5I GGATG 4 cut(s) 860, 868, 1048, 1276
BstFNI CGCG 2 cut(s) 347, 1587
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 5 cut(s) 91, 217, 967, 1239, 1308
BstMAI GTCTC 2 cut(s) 573, 1302
BstMBI GATC 5 cut(s) 88, 214, 964, 1236, 1305
BstMWI GCNNNNNNNGC 4 cut(s) 42, 929, 1550, 1584
BstNI CCWGG 2 cut(s) 225, 911
BstNSI RCATGY 2 cut(s) 156, 748
BstSCI CCNGG 3 cut(s) 223, 909, 1442
BstSFI CTRYAG 1 cut(s) 1292
BstSLI GKGCMC 1 cut(s) 494
BstUI CGCG 2 cut(s) 347, 1587
BstV1I GCAGC 6 cut(s) 7, 364, 683, 1357, 1553, 1565
BstV2I GAAGAC 2 cut(s) 1257, 1692
BstX2I RGATCY 1 cut(s) 1305
BstXI CCANNNNNNTGG 1 cut(s) 1531
BstYI RGATCY 1 cut(s) 1305
Bsu15I ATCGAT 1 cut(s) 967
BsuRI GGCC 2 cut(s) 1513, 1578
BsuTUI ATCGAT 1 cut(s) 967
BtgI CCRYGG 2 cut(s) 345, 924
BtrI CACGTC 1 cut(s) 1255
BtsCI GGATG 4 cut(s) 860, 868, 1048, 1276
BtsIMutI CAGTG 1 cut(s) 254
Cac8I GCNNGC 4 cut(s) 74, 1350, 1449, 1602
CfoI GCGC 1 cut(s) 604
Cfr13I GGNCC 3 cut(s) 193, 227, 721
Cfr42I CCGCGG 1 cut(s) 348
ClaI ATCGAT 1 cut(s) 967
CsiI ACCWGGT 1 cut(s) 909
Csp6I GTAC 1 cut(s) 1559
CviAII CATG 9 cut(s) 77, 153, 419, 523, 580, 745, 925, 1619, 1657
CviQI GTAC 1 cut(s) 1559
DdeI CTNAG 6 cut(s) 165, 940, 1005, 1353, 1596, 1710
DpnI GATC 5 cut(s) 90, 216, 966, 1238, 1307
DpnII GATC 5 cut(s) 88, 214, 964, 1236, 1305
EaeI YGGCCR 1 cut(s) 1576
Eam1104I CTCTTC 3 cut(s) 186, 321, 1359
EarI CTCTTC 3 cut(s) 186, 321, 1359
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 2 cut(s) 179, 828
Eco130I CCWWGG 2 cut(s) 924, 1301
Eco24I GRGCYC 3 cut(s) 181, 410, 830
Eco47I GGWCC 3 cut(s) 193, 227, 721
Eco53kI GAGCTC 2 cut(s) 179, 828
Eco57I CTGAAG 2 cut(s) 306, 1725
Eco88I CYCGRG 2 cut(s) 1428, 1628
EcoICRI GAGCTC 2 cut(s) 179, 828
EcoRI GAATTC 1 cut(s) 794
EcoRII CCWGG 2 cut(s) 223, 909
EcoT14I CCWWGG 2 cut(s) 924, 1301
EcoT38I GRGCYC 3 cut(s) 181, 410, 830
ErhI CCWWGG 2 cut(s) 924, 1301
FaeI CATG 9 cut(s) 80, 156, 422, 526, 583, 748, 928, 1622, 1660
FaqI GGGAC 2 cut(s) 365, 1629
FatI CATG 9 cut(s) 76, 152, 418, 522, 579, 744, 924, 1618, 1656
FauI CCCGC 1 cut(s) 340
FauNDI CATATG 1 cut(s) 1360
FbaI TGATCA 1 cut(s) 1236
Fnu4HI GCNGC 6 cut(s) 21, 378, 672, 1346, 1542, 1554
FokI GGATG 4 cut(s) 867, 875, 1055, 1283
FriOI GRGCYC 3 cut(s) 181, 410, 830
Fsp4HI GCNGC 6 cut(s) 21, 378, 672, 1346, 1542, 1554
FspBI CTAG 4 cut(s) 200, 392, 842, 1302
GlaI GCGC 1 cut(s) 603
GluI GCNGC 6 cut(s) 21, 378, 672, 1346, 1542, 1554
GsaI CCCAGC 1 cut(s) 862
HaeIII GGCC 2 cut(s) 1513, 1578
HapII CCGG 1 cut(s) 1443
HhaI GCGC 1 cut(s) 604
Hin1II CATG 9 cut(s) 80, 156, 422, 526, 583, 748, 928, 1622, 1660
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 3 cut(s) 353, 566, 1697
HpaII CCGG 1 cut(s) 1443
Hpy166II GTNNAC 2 cut(s) 415, 1567
Hpy8I GTNNAC 2 cut(s) 415, 1567
Hpy99I CGWCG 2 cut(s) 324, 806
HpyAV CCTTC 5 cut(s) 281, 1025, 1052, 1482, 1675
HpyCH4III ACNGT 1 cut(s) 1296
HpyCH4IV ACGT 3 cut(s) 804, 1254, 1581
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 929, 1550, 1584
HpyF3I CTNAG 6 cut(s) 165, 940, 1005, 1353, 1596, 1710
HpySE526I ACGT 3 cut(s) 804, 1254, 1581
Hsp92II CATG 9 cut(s) 80, 156, 422, 526, 583, 748, 928, 1622, 1660
HspAI GCGC 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 1236
KspI CCGCGG 1 cut(s) 348
Kzo9I GATC 5 cut(s) 88, 214, 964, 1236, 1305
LguI GCTCTTC 2 cut(s) 186, 321
LmnI GCTCC 2 cut(s) 1504, 1720
Lsp1109I GCAGC 6 cut(s) 7, 364, 683, 1357, 1553, 1565
LweI GCATC 5 cut(s) 432, 845, 1045, 1383, 1540
MabI ACCWGGT 1 cut(s) 909
MaeI CTAG 4 cut(s) 200, 392, 842, 1302
MaeII ACGT 3 cut(s) 804, 1254, 1581
MaeIII GTNAC 3 cut(s) 137, 589, 935
MalI GATC 5 cut(s) 90, 216, 966, 1238, 1307
MboI GATC 5 cut(s) 88, 214, 964, 1236, 1305
MboII GAAGA 7 cut(s) 173, 308, 347, 451, 1262, 1376, 1692
MfeI CAATTG 2 cut(s) 662, 1498
MflI RGATCY 1 cut(s) 1305
MhlI GDGCHC 4 cut(s) 181, 410, 494, 830
MlsI TGGCCA 1 cut(s) 1578
MluNI TGGCCA 1 cut(s) 1578
MlyI GAGTC 3 cut(s) 347, 575, 1706
MmeI TCCRAC 3 cut(s) 339, 811, 1677
Mox20I TGGCCA 1 cut(s) 1578
MroXI GAANNNNTTC 1 cut(s) 1415
MscI TGGCCA 1 cut(s) 1578
MseI TTAA 6 cut(s) 143, 302, 833, 978, 1083, 1280
MslI CAYNNNNRTG 1 cut(s) 1041
Msp20I TGGCCA 1 cut(s) 1578
MspA1I CMGCKG 4 cut(s) 347, 1348, 1531, 1556
MspI CCGG 1 cut(s) 1443
MspR9I CCNGG 3 cut(s) 225, 911, 1444
MunI CAATTG 2 cut(s) 662, 1498
Mva1269I GAATGC 3 cut(s) 760, 997, 1124
MvaI CCWGG 2 cut(s) 225, 911
MvnI CGCG 2 cut(s) 347, 1587
MwoI GCNNNNNNNGC 4 cut(s) 42, 929, 1550, 1584
NciI CCSGG 1 cut(s) 1444
NcoI CCATGG 1 cut(s) 924
NdeI CATATG 1 cut(s) 1360
NdeII GATC 5 cut(s) 88, 214, 964, 1236, 1305
NlaIII CATG 9 cut(s) 80, 156, 422, 526, 583, 748, 928, 1622, 1660
NlaIV GGNNCC 1 cut(s) 1307
NspI RCATGY 2 cut(s) 156, 748
PaeR7I CTCGAG 1 cut(s) 1628
PciSI GCTCTTC 2 cut(s) 186, 321
PctI GAATGC 3 cut(s) 760, 997, 1124
PdmI GAANNNNTTC 1 cut(s) 1415
PkrI GCNGC 6 cut(s) 22, 379, 673, 1347, 1543, 1555
PleI GAGTC 3 cut(s) 347, 574, 1705
PpsI GAGTC 3 cut(s) 347, 574, 1705
Psp124BI GAGCTC 2 cut(s) 181, 830
Psp6I CCWGG 2 cut(s) 223, 909
PspFI CCCAGC 1 cut(s) 858
PspGI CCWGG 2 cut(s) 223, 909
PspN4I GGNNCC 1 cut(s) 1307
PspPI GGNCC 3 cut(s) 193, 227, 721
PspXI VCTCGAGB 1 cut(s) 1628
PsuI RGATCY 1 cut(s) 1305
PvuII CAGCTG 3 cut(s) 1348, 1531, 1556
RsaI GTAC 1 cut(s) 1560
RsaNI GTAC 1 cut(s) 1559
RseI CAYNNNNRTG 1 cut(s) 1041
SacI GAGCTC 2 cut(s) 181, 830
SacII CCGCGG 1 cut(s) 348
SapI GCTCTTC 2 cut(s) 186, 321
SaqAI TTAA 6 cut(s) 143, 302, 833, 978, 1083, 1280
SatI GCNGC 6 cut(s) 21, 378, 672, 1346, 1542, 1554
Sau3AI GATC 5 cut(s) 88, 214, 964, 1236, 1305
Sau96I GGNCC 3 cut(s) 193, 227, 721
SchI GAGTC 3 cut(s) 347, 575, 1706
ScrFI CCNGG 3 cut(s) 225, 911, 1444
SduI GDGCHC 4 cut(s) 181, 410, 494, 830
SexAI ACCWGGT 1 cut(s) 909
SfaNI GCATC 5 cut(s) 432, 845, 1045, 1383, 1540
SfcI CTRYAG 1 cut(s) 1292
Sfr274I CTCGAG 1 cut(s) 1628
Sfr303I CCGCGG 1 cut(s) 348
SgrBI CCGCGG 1 cut(s) 348
SinI GGWCC 3 cut(s) 193, 227, 721
SlaI CTCGAG 1 cut(s) 1628
SmiMI CAYNNNNRTG 1 cut(s) 1041
SmlI CTYRAG 2 cut(s) 1112, 1628
SmoI CTYRAG 2 cut(s) 1112, 1628
SsiI CCGC 5 cut(s) 345, 347, 587, 616, 1587
SspI AATATT 1 cut(s) 475
SspMI CTAG 4 cut(s) 200, 392, 842, 1302
SstI GAGCTC 2 cut(s) 181, 830
StyD4I CCNGG 3 cut(s) 223, 909, 1442
StyI CCWWGG 2 cut(s) 924, 1301
TaaI ACNGT 1 cut(s) 1296
TaiI ACGT 3 cut(s) 807, 1257, 1584
TaqI TCGA 4 cut(s) 967, 1548, 1629, 1690
TatI WGTACW 1 cut(s) 1558
Tru1I TTAA 6 cut(s) 143, 302, 833, 978, 1083, 1280
Tru9I TTAA 6 cut(s) 143, 302, 833, 978, 1083, 1280
TscAI CASTG 1 cut(s) 254
TseI GCWGC 6 cut(s) 20, 377, 671, 1345, 1541, 1553
TspDTI ATGAA 9 cut(s) 106, 682, 797, 969, 1092, 1213, 1263, 1377, 1673
TspRI CASTG 1 cut(s) 254
VpaK11BI GGWCC 3 cut(s) 193, 227, 721
XapI RAATTY 2 cut(s) 533, 794
XbaI TCTAGA 1 cut(s) 391
XceI RCATGY 2 cut(s) 156, 748
XhoI CTCGAG 1 cut(s) 1628
XmaJI CCTAGG 1 cut(s) 1301
XmnI GAANNNNTTC 1 cut(s) 1415
XspI CTAG 4 cut(s) 200, 392, 842, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.