Rorug03G0328900

DNA-directed RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007390
Physical Location & Seq
Reverse (-)
37977716 .. 37980153
2438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0328900.1

Sequence Viewer

Length: 1197 bp
ATGGCGCTTGTGAAACCCATCACGAATTTCAGCAATGTCGTTACTCCCAAATTTGGGAATACCAGAAGCACTAGTAGCAAGTCGAGCACCATGATCAGAATGTCGTCGTCGTCGTCGTCCACCAAAAAGTCCACTACTAAGAAAGCCATCAAGGAGACGCTGTTGGCACCAAGGTTCTACACCACCGACTTTGATGAGATGGAGACCCTTTTCAACACTGAAATCAACAACAATTTGAACCAGGTTGAGTTTGAGGCTCTGCTTCAGGAGTTCAAGACCGACTACAACCAGACCCATTTTGTGAGGAACAAGGAGTTCAAGGTGGCTGCTGATAATCTCCAGGGCCCCCTCAGACAGATCTTTGTCGAGTTCTTGGAGAGGTCTTGTACTGCTGAGTTCTCTGGCTTCCTTCTTTACAAAGAGCTTGGAAGAAGGCTTAAGAAAACCAATCCAGTGGTAGCTGAGATATTCTCTCTCATGTCCAGGGATGAAGCCAGGCATGCCGGTTTCTTGAACAAAGGCCTGTCGGATTTCAATTTGGCATTGGACTTGGGGTTTCTGACCAAGGCAAGAAAATACACATTTTTCAAACCAAAGTTCATCTTCTATGCCACTTATTTGTCTGAGAAAATTGGATACTGGAGATACATTACCATATACAGGCATCTCAAGGCAAATCCCGAGTACCAGTGCTATCCCATTTTCAAGTATTTTGAGAATTGGTGCCAGGATGAGAACAGACATGGTGACTTCTTTTCTGCACTGATGAAGGCGCAACCCCAATTCCTGAATGACTGGAAGGCAAAGTTGTGGTCTCGATTCTTCTGCTTATCGGTATATGTGACAATGTACCTAAATGATTGCCAACGGACAGCTTTCTATGAAGGCATCGGGCTAAATACAAAAGAATTTGATATGCATGTCATCATTGAGACAAATCGCACAACAGCCAGAATTTTCCCAGCCGTGCTTGACGTTGAGAACCCAGAATTCAAAAGGAAACTTGACCGCATGGTGGAGATTAACCAGAAGCTCATTGCTGTTGGGGAAAGCGACGACATTCCCCTGGTGAAAAATTTGAGGAGGATACCAATTGTTGCTGCATTGGCCTCTGAACTTCTGGCTGCATACTTAATGCCACCCATTGAGTCTGGATCCGTCGATTTTGCGGAGTTTGAGCCCCAAGTGGTGTACTAA

Protein Analysis

398

Amino Acids

46.28

Weight (kDa)

8.83

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubrerythrin PF02915 123 - 256 3.7e-43 Rubrerythrin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 166, 723
AciI CCGC 2 cut(s) 1009, 1169
AclWI GGATC 2 cut(s) 1149, 1162
AcsI RAATTY 6 cut(s) 25, 50, 908, 954, 989, 1075
AcuI CTGAAG 1 cut(s) 248
AfaI GTAC 4 cut(s) 388, 686, 851, 1193
AfiI CCNNNNNNNGG 4 cut(s) 53, 54, 660, 1015
AflII CTTAAG 1 cut(s) 437
AgsI TTSAA 9 cut(s) 214, 238, 274, 319, 514, 535, 589, 706, 994
AhlI ACTAGT 1 cut(s) 71
AjnI CCWGG 6 cut(s) 240, 339, 482, 494, 726, 1065
AluBI AGCT 4 cut(s) 424, 461, 875, 1033
AluI AGCT 4 cut(s) 424, 461, 875, 1033
Alw21I GWGCWC 1 cut(s) 89
Alw26I GTCTC 4 cut(s) 149, 197, 819, 926
AlwI GGATC 2 cut(s) 1149, 1162
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 3 cut(s) 343, 520, 1107
ApaI GGGCCC 1 cut(s) 347
ApeKI GCWGC 3 cut(s) 326, 1100, 1124
ApoI RAATTY 6 cut(s) 25, 50, 908, 954, 989, 1075
ArsI GACNNNNNNTTYG 2 cut(s) 797, 829
AspLEI GCGC 2 cut(s) 7, 775
AspS9I GGNCC 2 cut(s) 343, 344
AsuHPI GGTGA 2 cut(s) 758, 1081
AvaI CYCGRG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 347
BamHI GGATCC 1 cut(s) 1154
BanI GGYRCC 2 cut(s) 166, 723
BanII GRGCYC 2 cut(s) 347, 1182
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 3 cut(s) 313, 1087, 1111
BccI CCATC 3 cut(s) 26, 155, 193
BceAI ACGGC 1 cut(s) 950
BcgI CGANNNNNNTGC 2 cut(s) 807, 841
BciT130I CCWGG 6 cut(s) 242, 341, 484, 496, 728, 1067
BciVI GTATCC 2 cut(s) 629, 1080
BclI TGATCA 1 cut(s) 93
BcoDI GTCTC 4 cut(s) 149, 197, 819, 926
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 1 cut(s) 72
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 437
BfuI GTATCC 2 cut(s) 629, 1080
BglII AGATCT 1 cut(s) 357
BisI GCNGC 3 cut(s) 327, 1101, 1125
BlsI GCNGC 3 cut(s) 328, 1102, 1126
Bme1390I CCNGG 6 cut(s) 242, 341, 484, 496, 728, 1067
BmeT110I CYCGRG 1 cut(s) 680
BmgT120I GGNCC 2 cut(s) 343, 344
BmiI GGNNCC 5 cut(s) 168, 345, 346, 725, 1156
BmrFI CCNGG 6 cut(s) 242, 341, 484, 496, 728, 1067
BmsI GCATC 2 cut(s) 673, 897
BplI GAGNNNNNCTC 2 cut(s) 455, 487
BpmI CTGGAG 2 cut(s) 323, 661
BpuEI CTTGAG 1 cut(s) 653
BsaI GGTCTC 2 cut(s) 197, 819
BsaJI CCNNGG 5 cut(s) 170, 340, 483, 564, 1065
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 4 cut(s) 53, 54, 660, 1015
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 4 cut(s) 452, 644, 688, 800
Bse3DI GCAATG 2 cut(s) 40, 1035
BseBI CCWGG 6 cut(s) 242, 341, 484, 496, 728, 1067
BseDI CCNNGG 5 cut(s) 170, 340, 483, 564, 1065
BseGI GGATG 2 cut(s) 493, 736
BseLI CCNNNNNNNGG 4 cut(s) 53, 54, 660, 1015
BseMI GCAATG 2 cut(s) 40, 1035
BseMII CTCAG 4 cut(s) 364, 384, 453, 615
BseNI ACTGG 4 cut(s) 452, 644, 688, 800
BseRI GAGGAG 1 cut(s) 1096
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 3 cut(s) 313, 1087, 1111
BseYI CCCAGC 1 cut(s) 961
BsgI GTGCAG 1 cut(s) 744
BshFI GGCC 3 cut(s) 345, 522, 1109
BshNI GGYRCC 2 cut(s) 166, 723
BsiHKAI GWGCWC 1 cut(s) 89
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 1 cut(s) 504
BslI CCNNNNNNNGG 4 cut(s) 53, 54, 660, 1015
BsmAI GTCTC 4 cut(s) 149, 197, 819, 926
BsmBI CGTCTC 1 cut(s) 149
BsnI GGCC 3 cut(s) 345, 522, 1109
Bso31I GGTCTC 2 cut(s) 197, 819
BsoBI CYCGRG 1 cut(s) 680
Bsp120I GGGCCC 1 cut(s) 343
Bsp1286I GDGCHC 3 cut(s) 89, 347, 1182
Bsp143I GATC 3 cut(s) 93, 357, 1154
BspACI CCGC 2 cut(s) 1009, 1169
BspANI GGCC 3 cut(s) 345, 522, 1109
BspCNI CTCAG 4 cut(s) 363, 385, 454, 616
BspLI GGNNCC 5 cut(s) 168, 345, 346, 725, 1156
BspPI GGATC 2 cut(s) 1149, 1162
BspT107I GGYRCC 2 cut(s) 166, 723
BspTI CTTAAG 1 cut(s) 437
BspTNI GGTCTC 2 cut(s) 197, 819
BsrDI GCAATG 2 cut(s) 40, 1035
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 4 cut(s) 452, 644, 688, 800
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 5 cut(s) 170, 340, 483, 564, 1065
BssMI GATC 3 cut(s) 93, 357, 1154
BssT1I CCWWGG 2 cut(s) 170, 564
Bst2UI CCWGG 6 cut(s) 242, 341, 484, 496, 728, 1067
BstAFI CTTAAG 1 cut(s) 437
BstC8I GCNNGC 1 cut(s) 501
BstDEI CTNAG 5 cut(s) 138, 350, 393, 462, 624
BstF5I GGATG 2 cut(s) 493, 736
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 775
BstKTI GATC 3 cut(s) 96, 360, 1157
BstMAI GTCTC 4 cut(s) 149, 197, 819, 926
BstMBI GATC 3 cut(s) 93, 357, 1154
BstMWI GCNNNNNNNGC 4 cut(s) 75, 84, 500, 1106
BstNI CCWGG 6 cut(s) 242, 341, 484, 496, 728, 1067
BstNSI RCATGY 2 cut(s) 503, 923
BstSCI CCNGG 6 cut(s) 240, 339, 482, 494, 726, 1065
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 3 cut(s) 313, 1087, 1111
BstX2I RGATCY 2 cut(s) 357, 1154
BstXI CCANNNNNNTGG 1 cut(s) 454
BstYI RGATCY 2 cut(s) 357, 1154
BsuI GTATCC 2 cut(s) 629, 1080
BsuRI GGCC 3 cut(s) 345, 522, 1109
BtsCI GGATG 2 cut(s) 493, 736
BtsIMutI CAGTG 4 cut(s) 216, 459, 695, 761
Cac8I GCNNGC 1 cut(s) 501
CfoI GCGC 2 cut(s) 7, 775
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 2 cut(s) 343, 344
CseI GACGC 1 cut(s) 166
CsiI ACCWGGT 1 cut(s) 240
Csp6I GTAC 4 cut(s) 387, 685, 850, 1192
CspCI CAANNNNNGTGG 2 cut(s) 172, 207
CviAII CATG 6 cut(s) 91, 478, 500, 743, 920, 1012
CviQI GTAC 4 cut(s) 387, 685, 850, 1192
DdeI CTNAG 5 cut(s) 138, 350, 393, 462, 624
DpnI GATC 3 cut(s) 95, 359, 1156
DpnII GATC 3 cut(s) 93, 357, 1154
Eco130I CCWWGG 2 cut(s) 170, 564
Eco147I AGGCCT 1 cut(s) 522
Eco24I GRGCYC 2 cut(s) 347, 1182
Eco31I GGTCTC 2 cut(s) 197, 819
Eco57I CTGAAG 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 680
EcoO109I RGGNCCY 2 cut(s) 343, 344
EcoRI GAATTC 1 cut(s) 989
EcoRII CCWGG 6 cut(s) 240, 339, 482, 494, 726, 1065
EcoT14I CCWWGG 2 cut(s) 170, 564
EcoT22I ATGCAT 1 cut(s) 921
EcoT38I GRGCYC 2 cut(s) 347, 1182
ErhI CCWWGG 2 cut(s) 170, 564
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 6 cut(s) 94, 481, 503, 746, 923, 1015
FalI AAGNNNNNCTT 2 cut(s) 587, 619
FatI CATG 6 cut(s) 90, 477, 499, 742, 919, 1011
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 327, 1101, 1125
FokI GGATG 2 cut(s) 500, 743
FriOI GRGCYC 2 cut(s) 347, 1182
Fsp4HI GCNGC 3 cut(s) 327, 1101, 1125
FspBI CTAG 1 cut(s) 72
GlaI GCGC 2 cut(s) 6, 774
GluI GCNGC 3 cut(s) 327, 1101, 1125
GsaI CCCAGC 1 cut(s) 965
GsuI CTGGAG 2 cut(s) 323, 661
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 345, 522, 1109
HapII CCGG 1 cut(s) 504
HgaI GACGC 1 cut(s) 166
HhaI GCGC 2 cut(s) 7, 775
Hin1II CATG 6 cut(s) 94, 481, 503, 746, 923, 1015
Hin6I GCGC 2 cut(s) 5, 773
HinP1I GCGC 2 cut(s) 5, 773
HinfI GANTC 2 cut(s) 819, 1148
HpaII CCGG 1 cut(s) 504
HphI GGTGA 2 cut(s) 758, 1081
Hpy166II GTNNAC 3 cut(s) 120, 132, 1192
Hpy188I TCNGA 6 cut(s) 98, 353, 529, 561, 625, 1114
Hpy188III TCNNGA 8 cut(s) 22, 266, 274, 511, 680, 787, 816, 1152
Hpy8I GTNNAC 3 cut(s) 120, 132, 1192
Hpy99I CGWCG 6 cut(s) 109, 112, 115, 118, 1058, 1163
HpyAV CCTTC 5 cut(s) 419, 426, 763, 793, 878
HpyCH4IV ACGT 1 cut(s) 975
HpyCH4V TGCA 4 cut(s) 761, 919, 1103, 1127
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 84, 500, 1106
HpyF3I CTNAG 5 cut(s) 138, 350, 393, 462, 624
HpySE526I ACGT 1 cut(s) 975
Hsp92II CATG 6 cut(s) 94, 481, 503, 746, 923, 1015
HspAI GCGC 2 cut(s) 5, 773
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 3 cut(s) 93, 357, 1154
Lsp1109I GCAGC 3 cut(s) 313, 1087, 1111
LweI GCATC 2 cut(s) 673, 897
MabI ACCWGGT 1 cut(s) 240
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 975
MaeIII GTNAC 3 cut(s) 40, 746, 841
MalI GATC 3 cut(s) 95, 359, 1156
MboI GATC 3 cut(s) 93, 357, 1154
MboII GAAGA 3 cut(s) 441, 595, 814
MfeI CAATTG 1 cut(s) 1092
MflI RGATCY 2 cut(s) 357, 1154
MhlI GDGCHC 3 cut(s) 89, 347, 1182
MlyI GAGTC 1 cut(s) 1157
MmeI TCCRAC 1 cut(s) 507
MnlI CCTC 7 cut(s) 247, 297, 359, 372, 1074, 1077, 1120
Mph1103I ATGCAT 1 cut(s) 921
MseI TTAA 3 cut(s) 438, 1023, 1133
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 6 cut(s) 242, 341, 484, 496, 728, 1067
MunI CAATTG 1 cut(s) 1092
MvaI CCWGG 6 cut(s) 242, 341, 484, 496, 728, 1067
MwoI GCNNNNNNNGC 4 cut(s) 75, 84, 500, 1106
NdeII GATC 3 cut(s) 93, 357, 1154
NlaIII CATG 6 cut(s) 94, 481, 503, 746, 923, 1015
NlaIV GGNNCC 5 cut(s) 168, 345, 346, 725, 1156
NmuCI GTSAC 2 cut(s) 746, 841
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 2 cut(s) 503, 923
PaeI GCATGC 1 cut(s) 503
PceI AGGCCT 1 cut(s) 522
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 113
PfeI GAWTC 1 cut(s) 819
PkrI GCNGC 3 cut(s) 328, 1102, 1126
PleI GAGTC 1 cut(s) 1156
PpsI GAGTC 1 cut(s) 1156
Psp6I CCWGG 6 cut(s) 240, 339, 482, 494, 726, 1065
PspFI CCCAGC 1 cut(s) 961
PspGI CCWGG 6 cut(s) 240, 339, 482, 494, 726, 1065
PspN4I GGNNCC 5 cut(s) 168, 345, 346, 725, 1156
PspOMI GGGCCC 1 cut(s) 343
PspPI GGNCC 2 cut(s) 343, 344
PsuI RGATCY 2 cut(s) 357, 1154
RsaI GTAC 4 cut(s) 388, 686, 851, 1193
RsaNI GTAC 4 cut(s) 387, 685, 850, 1192
SaqAI TTAA 3 cut(s) 438, 1023, 1133
SatI GCNGC 3 cut(s) 327, 1101, 1125
Sau3AI GATC 3 cut(s) 93, 357, 1154
Sau96I GGNCC 2 cut(s) 343, 344
SchI GAGTC 1 cut(s) 1157
ScrFI CCNGG 6 cut(s) 242, 341, 484, 496, 728, 1067
SduI GDGCHC 3 cut(s) 89, 347, 1182
SexAI ACCWGGT 1 cut(s) 240
SfaNI GCATC 2 cut(s) 673, 897
SmlI CTYRAG 2 cut(s) 437, 668
SmoI CTYRAG 2 cut(s) 437, 668
SpeI ACTAGT 1 cut(s) 71
SphI GCATGC 1 cut(s) 503
SseBI AGGCCT 1 cut(s) 522
SsiI CCGC 2 cut(s) 1009, 1169
SspMI CTAG 1 cut(s) 72
StuI AGGCCT 1 cut(s) 522
StyD4I CCNGG 6 cut(s) 240, 339, 482, 494, 726, 1065
StyI CCWWGG 2 cut(s) 170, 564
TaiI ACGT 1 cut(s) 978
TaqI TCGA 4 cut(s) 83, 366, 817, 1161
TaqII GACCGA 1 cut(s) 293
TatI WGTACW 2 cut(s) 386, 1191
TfiI GAWTC 1 cut(s) 819
Tru1I TTAA 3 cut(s) 438, 1023, 1133
Tru9I TTAA 3 cut(s) 438, 1023, 1133
TscAI CASTG 4 cut(s) 223, 459, 695, 768
TseFI GTSAC 2 cut(s) 746, 841
TseI GCWGC 3 cut(s) 326, 1100, 1124
Tsp45I GTSAC 2 cut(s) 746, 841
TspDTI ATGAA 4 cut(s) 504, 589, 782, 897
TspGWI ACGGA 2 cut(s) 883, 1147
TspRI CASTG 4 cut(s) 223, 459, 695, 768
Vha464I CTTAAG 1 cut(s) 437
XapI RAATTY 6 cut(s) 25, 50, 908, 954, 989, 1075
XceI RCATGY 2 cut(s) 503, 923
XspI CTAG 1 cut(s) 72
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.