Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0006834

Physical Location & Seq

Genomic Coordinates Reverse (-)

40818729 .. 40820268

Gene Structure 1540 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0240000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1044 bp

ATGGCCTTGTGGGGAGCTTTATCTCCTCACCTTCTACTCCTCGGCCTCATGTGCATCCTCCTCTACCCCAACGTTGGCATTCGCCTCGGCTCTGTCCGTCGTCCCTCCCCGGACGTCCCCGTCTTTCGGGAAGCCCCGGCCTTTCGGAACGGTGACCAATGCAGCTCGGGAAATAACACTGACGATATCCATGTGGCCATGACCCTCGACGCCAATTACATCCGGGGCACGATGGCTGCTGTCTTCTCTTTGTTGCAACATTCTACCTGCCCTGAAAACCTCAAGTTCCACTTTCTATCTGCCCATGTTGCGCCGGAGCTATTTTCGAGAATCAACTCCACGTTTCCTTACTTGGACTTCAAGACTTACCCCTTTGATTCAAACAGAGTTCGTGGGAAGATATCAAGGTCCATAAGACGAGCATTGGATCAACCTATAAATTATGCGAGAATTTACCTCGCTGATCTTCTTCCAAGTGATATTAGACGTGTTATTTACTTGGATTCAGACCTCGTTGTGGTCGACGACATTGCGAAGCTGTGGGGGGTGGACATGGAAGACAAGGTGGTGGCTGCCCCAGAATATTGTCAAGCAAATTTCACACAATACTTCACAGATGCATTATGGTCCGACCCGGATTTGTCAAAGACGTTCCGAGGAAGAAACCCATGTTATTTCAACACAGGAGTGATGGTGGTGGATGTGGATAAATGGAGAAAAGGAGGGTATACACAGAAAGTAGAGGAGTGGATGGCCCTGCAAAAGAGAAAGAGGCTATACCATTTGGGGTCTTTGCCGCCATTTTTGCTGGTTTTGGCTGGGAACATCAAGGCTGTGGATCACAGGTGGAACCAACATGGGCTTGGTGGTGACAACTTTGAAGGAAAGTGTAGGCGCCTCCACCCAGGTCCCATTAGTCTTCTTCATTGGAGCGGAAAGGGTAAGCCATGGTTGCGGTTGGATTCTAGGAGAACATGTACTGTCGATCATCTTTGGGCGCCATACGATCTCTACCAGTCTTCGAGAAGCTTTCTGGAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009965 GO:0009966 GO:0009987 GO:0010016 GO:0010051 GO:0010305 GO:0010498 GO:0010646 GO:0010928 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0023051 GO:0030163 GO:0031146 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0048366 GO:0048367 GO:0048583 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051603 GO:0060776 GO:0065007 GO:0070647 GO:0071704 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box

Protein Analysis

347

Amino Acids

39.65

Weight (kDa)

8.87

Isoelectric Point (pI)

46.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_8	PF01501	64 - 319	8e-59	Glycosyl transferase family 8
Glyco_transf_24	PF18404	78 - 242	3.5e-09	Glucosyltransferase 24

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006834)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27340
fragaria_vesca	FvH4_4g23620 FvH4_4g23620 FvH4_4g23620
malus_domestica	MD13G1120600.v1.1
prunus_persica	Prupe.1G224600_v2.0.a1 Prupe.1G224600_v2.0.a1 Prupe.1G224600_v2.0.a1 Prupe.1G224600_v2.0.a1 Prupe.1G224600_v2.0.a1 Prupe.1G224600_v2.0.a1 Prupe.1G224600_v2.0.a1
pyrus_communis	pycom13g10500 pycom16g10300
rosa_chinensis	RchiOBHm_Chr4g0430081
rosa_laevigata	RLG00000007005
rosa_multiflora	Rmu_ssc0000150.1_g000027
rosa_roxburghii	Rroxscaffold_5G00371890
rosa_rugosa	Rorug04G0239900 Rorug04G0239900 Rorug04G0240000
rosa_samantha	Rh4AG297000 Rh4BG303500 Rh4CG319000 Rh4DG300700
rosa_wichuraiana	Rw4G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	119
AatII	GACGTC	1 cut(s)	117
Acc36I	ACCTGC	1 cut(s)	275
AccB1I	GGYRCC	2 cut(s)	894, 997
AccBSI	CCGCTC	1 cut(s)	933
AccI	GTMKAC	2 cut(s)	522, 728
AciI	CCGC	3 cut(s)	797, 933, 955
AclI	AACGTT	1 cut(s)	72
AclWI	GGATC	2 cut(s)	435, 846
AcoI	YGGCCR	1 cut(s)	195
AcsI	RAATTY	2 cut(s)	450, 595
AcyI	GRCGYC	4 cut(s)	114, 210, 895, 998
AfaI	GTAC	1 cut(s)	979
AfiI	CCNNNNNNNGG	3 cut(s)	74, 126, 517
AflIII	ACRYGT	2 cut(s)	487, 974
AgsI	TTSAA	4 cut(s)	361, 381, 679, 881
AjiI	CACGTC	1 cut(s)	488
AjnI	CCWGG	1 cut(s)	904
AleI	CACNNNNGTG	1 cut(s)	686
AluBI	AGCT	5 cut(s)	17, 165, 319, 538, 1029
AluI	AGCT	5 cut(s)	17, 165, 319, 538, 1029
AlwI	GGATC	2 cut(s)	435, 846
Ama87I	CYCGRG	1 cut(s)	166
AoxI	GGCC	5 cut(s)	3, 43, 138, 195, 753
ApeKI	GCWGC	3 cut(s)	162, 236, 572
ApoI	RAATTY	2 cut(s)	450, 595
AspLEI	GCGC	3 cut(s)	313, 897, 1000
AspS9I	GGNCC	4 cut(s)	408, 627, 754, 908
AsuC2I	CCSGG	4 cut(s)	110, 137, 224, 635
AsuHPI	GGTGA	3 cut(s)	20, 164, 881
AvaI	CYCGRG	1 cut(s)	166
AvaII	GGWCC	3 cut(s)	408, 627, 908
BaeGI	GKGCMC	1 cut(s)	230
BalI	TGGCCA	1 cut(s)	197
BanI	GGYRCC	2 cut(s)	894, 997
BbsI	GAAGAC	4 cut(s)	235, 564, 911, 1011
BbvI	GCAGC	3 cut(s)	174, 223, 559
BccI	CCATC	3 cut(s)	226, 685, 745
BcgI	CGANNNNNNTGC	4 cut(s)	67, 101, 512, 546
BciT130I	CCWGG	1 cut(s)	906
BcnI	CCSGG	4 cut(s)	110, 137, 224, 635
BfaI	CTAG	1 cut(s)	966
BfoI	RGCGCY	2 cut(s)	898, 1001
BfuAI	ACCTGC	1 cut(s)	275
BisI	GCNGC	4 cut(s)	163, 237, 573, 797
BlsI	GCNGC	4 cut(s)	164, 238, 574, 798
Bme1390I	CCNGG	5 cut(s)	110, 137, 224, 635, 906
Bme18I	GGWCC	3 cut(s)	408, 627, 908
BmeT110I	CYCGRG	1 cut(s)	166
BmgBI	CACGTC	1 cut(s)	488
BmgT120I	GGNCC	4 cut(s)	408, 627, 754, 908
BmiI	GGNNCC	4 cut(s)	851, 896, 910, 999
BmrFI	CCNGG	5 cut(s)	110, 137, 224, 635, 906
BmsI	GCATC	2 cut(s)	63, 607
BpiI	GAAGAC	4 cut(s)	235, 564, 911, 1011
BpuEI	CTTGAG	1 cut(s)	266
BpuMI	CCSGG	4 cut(s)	110, 137, 224, 635
BsaHI	GRCGYC	4 cut(s)	114, 210, 895, 998
BsaJI	CCNNGG	8 cut(s)	40, 85, 108, 135, 223, 655, 904, 947
BsaXI	ACNNNNNCTCC	4 cut(s)	678, 708, 961, 991
Bsc4I	CCNNNNNNNGG	3 cut(s)	74, 126, 517
Bse1I	ACTGG	1 cut(s)	1015
Bse3DI	GCAATG	1 cut(s)	528
BseBI	CCWGG	1 cut(s)	906
BseDI	CCNNGG	8 cut(s)	40, 85, 108, 135, 223, 655, 904, 947
BseGI	GGATG	4 cut(s)	54, 219, 706, 756
BseLI	CCNNNNNNNGG	3 cut(s)	74, 126, 517
BseMI	GCAATG	1 cut(s)	528
BseNI	ACTGG	1 cut(s)	1015
BseRI	GAGGAG	4 cut(s)	15, 29, 50, 758
BseSI	GKGCMC	1 cut(s)	230
BseXI	GCAGC	3 cut(s)	174, 223, 559
BseYI	CCCAGC	1 cut(s)	818
BshFI	GGCC	5 cut(s)	5, 45, 140, 197, 755
BshNI	GGYRCC	2 cut(s)	894, 997
BsiHKCI	CYCGRG	1 cut(s)	166
BsiSI	CCGG	5 cut(s)	110, 137, 223, 314, 635
BslFI	GGGAC	3 cut(s)	87, 101, 894
BslI	CCNNNNNNNGG	3 cut(s)	74, 126, 517
BsmFI	GGGAC	3 cut(s)	87, 101, 894
BsmI	GAATGC	1 cut(s)	78
BsnI	GGCC	5 cut(s)	5, 45, 140, 197, 755
BsoBI	CYCGRG	1 cut(s)	166
Bsp1286I	GDGCHC	1 cut(s)	230
Bsp143I	GATC	5 cut(s)	427, 463, 838, 985, 1006
Bsp19I	CCATGG	1 cut(s)	947
BspACI	CCGC	3 cut(s)	797, 933, 955
BspANI	GGCC	5 cut(s)	5, 45, 140, 197, 755
BspLI	GGNNCC	4 cut(s)	851, 896, 910, 999
BspMI	ACCTGC	1 cut(s)	275
BspPI	GGATC	2 cut(s)	435, 846
BspT107I	GGYRCC	2 cut(s)	894, 997
BsrBI	CCGCTC	1 cut(s)	933
BsrDI	GCAATG	1 cut(s)	528
BsrI	ACTGG	1 cut(s)	1015
BssECI	CCNNGG	8 cut(s)	40, 85, 108, 135, 223, 655, 904, 947
BssMI	GATC	5 cut(s)	427, 463, 838, 985, 1006
BssNAI	GTATAC	1 cut(s)	729
BssNI	GRCGYC	4 cut(s)	114, 210, 895, 998
BssT1I	CCWWGG	1 cut(s)	947
Bst1107I	GTATAC	1 cut(s)	729
Bst2UI	CCWGG	1 cut(s)	906
Bst4CI	ACNGT	2 cut(s)	152, 982
BstACI	GRCGYC	4 cut(s)	114, 210, 895, 998
BstDSI	CCRYGG	1 cut(s)	947
BstEII	GGTNACC	1 cut(s)	152
BstF5I	GGATG	4 cut(s)	54, 219, 706, 756
BstH2I	RGCGCY	2 cut(s)	898, 1001
BstHHI	GCGC	3 cut(s)	313, 897, 1000
BstKTI	GATC	5 cut(s)	430, 466, 841, 988, 1009
BstMBI	GATC	5 cut(s)	427, 463, 838, 985, 1006
BstMWI	GCNNNNNNNGC	4 cut(s)	51, 308, 805, 952
BstNI	CCWGG	1 cut(s)	906
BstNSI	RCATGY	1 cut(s)	978
BstPI	GGTNACC	1 cut(s)	152
BstSCI	CCNGG	5 cut(s)	108, 135, 222, 633, 904
BstSLI	GKGCMC	1 cut(s)	230
BstV1I	GCAGC	3 cut(s)	174, 223, 559
BstV2I	GAAGAC	4 cut(s)	235, 564, 911, 1011
BstZ17I	GTATAC	1 cut(s)	729
BsuRI	GGCC	5 cut(s)	5, 45, 140, 197, 755
BtgI	CCRYGG	1 cut(s)	947
BtrI	CACGTC	1 cut(s)	488
BtsCI	GGATG	4 cut(s)	54, 219, 706, 756
BtsIMutI	CAGTG	1 cut(s)	177
BveI	ACCTGC	1 cut(s)	275
CfoI	GCGC	3 cut(s)	313, 897, 1000
Cfr13I	GGNCC	4 cut(s)	408, 627, 754, 908
CseI	GACGC	1 cut(s)	218
Csp6I	GTAC	1 cut(s)	978
CviAII	CATG	9 cut(s)	49, 191, 199, 305, 553, 669, 857, 948, 975
CviQI	GTAC	1 cut(s)	978
DinI	GGCGCC	2 cut(s)	896, 999
DpnI	GATC	5 cut(s)	429, 465, 840, 987, 1008
DpnII	GATC	5 cut(s)	427, 463, 838, 985, 1006
DrdI	GACNNNNNNGTC	1 cut(s)	119
DseDI	GACNNNNNNGTC	1 cut(s)	119
EaeI	YGGCCR	1 cut(s)	195
Eco130I	CCWWGG	1 cut(s)	947
Eco32I	GATATC	2 cut(s)	187, 402
Eco47I	GGWCC	3 cut(s)	408, 627, 908
Eco88I	CYCGRG	1 cut(s)	166
Eco91I	GGTNACC	1 cut(s)	152
EcoO109I	RGGNCCY	1 cut(s)	908
EcoO65I	GGTNACC	1 cut(s)	152
EcoRII	CCWGG	1 cut(s)	904
EcoRV	GATATC	2 cut(s)	187, 402
EcoT14I	CCWWGG	1 cut(s)	947
EcoT22I	ATGCAT	1 cut(s)	622
EgeI	GGCGCC	2 cut(s)	896, 999
EheI	GGCGCC	2 cut(s)	896, 999
ErhI	CCWWGG	1 cut(s)	947
FaeI	CATG	9 cut(s)	52, 194, 202, 308, 556, 672, 860, 951, 978
FalI	AAGNNNNNCTT	2 cut(s)	275, 307
FaqI	GGGAC	3 cut(s)	87, 101, 894
FatI	CATG	9 cut(s)	48, 190, 198, 304, 552, 668, 856, 947, 974
FblI	GTMKAC	2 cut(s)	522, 728
Fnu4HI	GCNGC	4 cut(s)	163, 237, 573, 797
FokI	GGATG	4 cut(s)	41, 206, 713, 763
Fsp4HI	GCNGC	4 cut(s)	163, 237, 573, 797
FspBI	CTAG	1 cut(s)	966
GlaI	GCGC	3 cut(s)	312, 896, 999
GluI	GCNGC	4 cut(s)	163, 237, 573, 797
GsaI	CCCAGC	1 cut(s)	822
HaeII	RGCGCY	2 cut(s)	898, 1001
HaeIII	GGCC	5 cut(s)	5, 45, 140, 197, 755
HapII	CCGG	5 cut(s)	110, 137, 223, 314, 635
HgaI	GACGC	1 cut(s)	218
HhaI	GCGC	3 cut(s)	313, 897, 1000
Hin1I	GRCGYC	4 cut(s)	114, 210, 895, 998
Hin1II	CATG	9 cut(s)	52, 194, 202, 308, 556, 672, 860, 951, 978
Hin6I	GCGC	3 cut(s)	311, 895, 998
HinP1I	GCGC	3 cut(s)	311, 895, 998
HincII	GTYRAC	1 cut(s)	523
HindII	GTYRAC	1 cut(s)	523
HindIII	AAGCTT	1 cut(s)	1027
HinfI	GANTC	4 cut(s)	330, 377, 503, 962
HpaII	CCGG	5 cut(s)	110, 137, 223, 314, 635
HphI	GGTGA	3 cut(s)	20, 164, 881
Hpy166II	GTNNAC	3 cut(s)	523, 550, 729
Hpy188I	TCNGA	4 cut(s)	147, 508, 631, 656
Hpy188III	TCNNGA	6 cut(s)	128, 168, 327, 361, 1023, 1034
Hpy8I	GTNNAC	3 cut(s)	523, 550, 729
Hpy99I	CGWCG	3 cut(s)	102, 212, 527
HpyAV	CCTTC	2 cut(s)	41, 875
HpyCH4III	ACNGT	2 cut(s)	152, 982
HpyCH4IV	ACGT	5 cut(s)	72, 114, 341, 487, 650
HpyCH4V	TGCA	5 cut(s)	54, 162, 256, 620, 760
HpyF10VI	GCNNNNNNNGC	4 cut(s)	51, 308, 805, 952
HpySE526I	ACGT	5 cut(s)	72, 114, 341, 487, 650
Hsp92I	GRCGYC	4 cut(s)	114, 210, 895, 998
Hsp92II	CATG	9 cut(s)	52, 194, 202, 308, 556, 672, 860, 951, 978
HspAI	GCGC	3 cut(s)	311, 895, 998
KasI	GGCGCC	2 cut(s)	894, 997
Kzo9I	GATC	5 cut(s)	427, 463, 838, 985, 1006
LmnI	GCTCC	3 cut(s)	14, 316, 930
Lsp1109I	GCAGC	3 cut(s)	174, 223, 559
LweI	GCATC	2 cut(s)	63, 607
MaeI	CTAG	1 cut(s)	966
MaeII	ACGT	5 cut(s)	72, 114, 341, 487, 650
MaeIII	GTNAC	2 cut(s)	152, 869
MalI	GATC	5 cut(s)	429, 465, 840, 987, 1008
MbiI	CCGCTC	1 cut(s)	933
MboI	GATC	5 cut(s)	427, 463, 838, 985, 1006
MboII	GAAGA	9 cut(s)	235, 409, 458, 461, 569, 672, 911, 914, 1011
MhlI	GDGCHC	1 cut(s)	230
MlsI	TGGCCA	1 cut(s)	197
MluCI	AATT	4 cut(s)	214, 439, 450, 595
MluNI	TGGCCA	1 cut(s)	197
Mly113I	GGCGCC	2 cut(s)	895, 998
MmeI	TCCRAC	2 cut(s)	654, 939
Mox20I	TGGCCA	1 cut(s)	197
Mph1103I	ATGCAT	1 cut(s)	622
MscI	TGGCCA	1 cut(s)	197
MslI	CAYNNNNRTG	1 cut(s)	686
Msp20I	TGGCCA	1 cut(s)	197
MspI	CCGG	5 cut(s)	110, 137, 223, 314, 635
MspR9I	CCNGG	5 cut(s)	110, 137, 224, 635, 906
Mva1269I	GAATGC	1 cut(s)	78
MvaI	CCWGG	1 cut(s)	906
MwoI	GCNNNNNNNGC	4 cut(s)	51, 308, 805, 952
NarI	GGCGCC	2 cut(s)	895, 998
NciI	CCSGG	4 cut(s)	110, 137, 224, 635
NcoI	CCATGG	1 cut(s)	947
NdeII	GATC	5 cut(s)	427, 463, 838, 985, 1006
NlaIII	CATG	9 cut(s)	52, 194, 202, 308, 556, 672, 860, 951, 978
NlaIV	GGNNCC	4 cut(s)	851, 896, 910, 999
NmeAIII	GCCGAG	2 cut(s)	21, 66
NmuCI	GTSAC	2 cut(s)	152, 869
NsiI	ATGCAT	1 cut(s)	622
NspI	RCATGY	1 cut(s)	978
OliI	CACNNNNGTG	1 cut(s)	686
PciI	ACATGT	1 cut(s)	974
PcsI	WCGNNNNNNNCGW	1 cut(s)	519
PctI	GAATGC	1 cut(s)	78
PfeI	GAWTC	4 cut(s)	330, 377, 503, 962
PkrI	GCNGC	4 cut(s)	164, 238, 574, 798
PluTI	GGCGCC	2 cut(s)	898, 1001
PpuMI	RGGWCCY	1 cut(s)	908
PscI	ACATGT	1 cut(s)	974
Psp1406I	AACGTT	1 cut(s)	72
Psp5II	RGGWCCY	1 cut(s)	908
Psp6I	CCWGG	1 cut(s)	904
PspEI	GGTNACC	1 cut(s)	152
PspFI	CCCAGC	1 cut(s)	818
PspGI	CCWGG	1 cut(s)	904
PspN4I	GGNNCC	4 cut(s)	851, 896, 910, 999
PspPI	GGNCC	4 cut(s)	408, 627, 754, 908
PspPPI	RGGWCCY	1 cut(s)	908
RsaI	GTAC	1 cut(s)	979
RsaNI	GTAC	1 cut(s)	978
RseI	CAYNNNNRTG	1 cut(s)	686
SalI	GTCGAC	1 cut(s)	521
SatI	GCNGC	4 cut(s)	163, 237, 573, 797
Sau3AI	GATC	5 cut(s)	427, 463, 838, 985, 1006
Sau96I	GGNCC	4 cut(s)	408, 627, 754, 908
ScrFI	CCNGG	5 cut(s)	110, 137, 224, 635, 906
SduI	GDGCHC	1 cut(s)	230
SfaNI	GCATC	2 cut(s)	63, 607
SfoI	GGCGCC	2 cut(s)	896, 999
SinI	GGWCC	3 cut(s)	408, 627, 908
SmiMI	CAYNNNNRTG	1 cut(s)	686
SmlI	CTYRAG	1 cut(s)	281
SmoI	CTYRAG	1 cut(s)	281
Sse9I	AATT	4 cut(s)	214, 439, 450, 595
SsiI	CCGC	3 cut(s)	797, 933, 955
SspDI	GGCGCC	2 cut(s)	894, 997
SspI	AATATT	1 cut(s)	584
SspMI	CTAG	1 cut(s)	966
StyD4I	CCNGG	5 cut(s)	108, 135, 222, 633, 904
StyI	CCWWGG	1 cut(s)	947
TaaI	ACNGT	2 cut(s)	152, 982
TaiI	ACGT	5 cut(s)	75, 117, 344, 490, 653
TaqI	TCGA	5 cut(s)	207, 326, 522, 984, 1022
TasI	AATT	4 cut(s)	214, 439, 450, 595
TatI	WGTACW	1 cut(s)	977
TauI	GCSGC	1 cut(s)	799
TfiI	GAWTC	4 cut(s)	330, 377, 503, 962
TscAI	CASTG	1 cut(s)	184
TseFI	GTSAC	2 cut(s)	152, 869
TseI	GCWGC	3 cut(s)	162, 236, 572
Tsp45I	GTSAC	2 cut(s)	152, 869
TspDTI	ATGAA	1 cut(s)	914
TspGWI	ACGGA	1 cut(s)	86
TspRI	CASTG	1 cut(s)	184
VpaK11BI	GGWCC	3 cut(s)	408, 627, 908
XapI	RAATTY	2 cut(s)	450, 595
XceI	RCATGY	1 cut(s)	978
XcmI	CCANNNNNNNNNTGG	1 cut(s)	860
XmiI	GTMKAC	2 cut(s)	522, 728
XspI	CTAG	1 cut(s)	966
ZraI	GACGTC	1 cut(s)	115
Zsp2I	ATGCAT	1 cut(s)	622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.