Rorug04G0242300

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016829
Physical Location & Seq
Reverse (-)
41096102 .. 41097125
1024 bp
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UTR
Exon/CDS
Intron
Rorug04G0242300.1

Sequence Viewer

Length: 555 bp
ATGGGTGTCAAAGACATTGCATTAACAGGTGTAGGCAACCTCATCAAGCTCCTCCCTACCGGCACGGTATTCTTGTTCCAGTTCCTCAACCCCGTTTTAACCAACAATGGCCAATGCAACATGTCCGTCAACAAGATTCTAAGCGCCATTTTCATTGCCGTTTCCGCCTTCTCTTGTTGCTTTGCTTCCTTCACCGACAGTTACACCGGCAGTGATGGACAAACTCACTATGGAATTGCAACAACAAAGGGTCTTTGGCCCTCAACCAGTTCTGGCTTTGTGGACTTGTCTGCTTACAAGCTTCGGGTTGGGGACTTTGTGCATGCCTTCTTTGCCTTGATCGTATTTGCAGTGGTATCACTTTTGGATTCCAACACTGTGAGGTGCTTCTATCCTGCATTTGAGTCCACTGAGAAGGTTCTGCTGCAGGCGTTGCCTCCTGTAATTGGCACTATTGCTGGTACTGTCTTTGTTGTGTTCCCTAATACACGCCACGGAATTGGGTACCCTTATTCAAGTTCTAATTCTTCACAAGACTCGGAGGACTCCTCCTAA

Protein Analysis

184

Amino Acids

19.67

Weight (kDa)

5.81

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 10 - 169 1.2e-62 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016829)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23780
rosa_chinensis RchiOBHm_Chr4g0430271
rosa_laevigata RLG00000006989
rosa_multiflora Rmu_sc0001089.1_g000025 Rmu_sc0027338.1_g000002
rosa_roxburghii Rroxscaffold_5G00372070
rosa_rugosa Rorug04G0242200 Rorug04G0242300 Rorug04G0242400
rosa_samantha Rh4AG298700 Rh4BG305300 Rh4CG320800 Rh4DG302600
rosa_wichuraiana Rw4G025900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 504
AciI CCGC 1 cut(s) 165
AcoI YGGCCR 1 cut(s) 109
AfaI GTAC 2 cut(s) 463, 506
AfiI CCNNNNNNNGG 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 120
AgsI TTSAA 1 cut(s) 516
AluBI AGCT 2 cut(s) 49, 301
AluI AGCT 2 cut(s) 49, 301
AoxI GGCC 2 cut(s) 109, 257
ApeKI GCWGC 1 cut(s) 424
Asp718I GGTACC 1 cut(s) 504
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 258
AsuHPI GGTGA 1 cut(s) 184
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 1 cut(s) 504
BbvI GCAGC 1 cut(s) 411
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 425
BfoI RGCGCY 1 cut(s) 147
BisI GCNGC 1 cut(s) 425
BlsI GCNGC 1 cut(s) 426
BmgT120I GGNCC 1 cut(s) 258
BmiI GGNNCC 1 cut(s) 506
BplI GAGNNNNNCTC 1 cut(s) 533
BsaJI CCNNGG 1 cut(s) 493
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse118I RCCGGY 2 cut(s) 59, 206
Bse1I ACTGG 2 cut(s) 79, 267
Bse3DI GCAATG 2 cut(s) 15, 153
BseDI CCNNGG 1 cut(s) 493
BseLI CCNNNNNNNGG 1 cut(s) 446
BseMI GCAATG 2 cut(s) 15, 153
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 2 cut(s) 79, 267
BseRI GAGGAG 2 cut(s) 41, 538
BseXI GCAGC 1 cut(s) 411
BshFI GGCC 2 cut(s) 111, 259
BshNI GGYRCC 1 cut(s) 504
BsiSI CCGG 2 cut(s) 60, 207
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 2 cut(s) 111, 259
Bsp143I GATC 1 cut(s) 339
BspACI CCGC 1 cut(s) 165
BspANI GGCC 2 cut(s) 111, 259
BspCNI CTCAG 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 506
BspMAI CTGCAG 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 504
BsrDI GCAATG 2 cut(s) 15, 153
BsrFI RCCGGY 2 cut(s) 59, 206
BsrI ACTGG 2 cut(s) 79, 267
BssAI RCCGGY 2 cut(s) 59, 206
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 1 cut(s) 339
Bst4CI ACNGT 4 cut(s) 67, 200, 379, 466
BstAPI GCANNNNNTGC 1 cut(s) 433
BstC8I GCNNGC 2 cut(s) 324, 429
BstDEI CTNAG 2 cut(s) 140, 411
BstDSI CCRYGG 1 cut(s) 493
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 1 cut(s) 342
BstMBI GATC 1 cut(s) 339
BstMWI GCNNNNNNNGC 3 cut(s) 164, 332, 433
BstNSI RCATGY 2 cut(s) 124, 326
BstSFI CTRYAG 1 cut(s) 425
BstV1I GCAGC 1 cut(s) 411
BstXI CCANNNNNNTGG 1 cut(s) 500
BsuRI GGCC 2 cut(s) 111, 259
BtgI CCRYGG 1 cut(s) 493
BtsI GCAGTG 2 cut(s) 217, 357
BtsIMutI CAGTG 4 cut(s) 217, 357, 375, 408
Cac8I GCNNGC 2 cut(s) 324, 429
CfoI GCGC 1 cut(s) 146
Cfr10I RCCGGY 2 cut(s) 59, 206
Cfr13I GGNCC 1 cut(s) 258
Csp6I GTAC 2 cut(s) 462, 505
CviAII CATG 2 cut(s) 121, 323
CviJI RGCY 5 cut(s) 49, 111, 259, 276, 301
CviKI_1 RGCY 5 cut(s) 49, 111, 259, 276, 301
CviQI GTAC 2 cut(s) 462, 505
DdeI CTNAG 2 cut(s) 140, 411
DpnI GATC 1 cut(s) 341
DpnII GATC 1 cut(s) 339
EaeI YGGCCR 1 cut(s) 109
EciI GGCGGA 1 cut(s) 154
FaeI CATG 2 cut(s) 124, 326
FaiI YATR 3 cut(s) 122, 231, 324
FaqI GGGAC 1 cut(s) 326
FatI CATG 2 cut(s) 120, 322
Fnu4HI GCNGC 1 cut(s) 425
Fsp4HI GCNGC 1 cut(s) 425
GlaI GCGC 1 cut(s) 145
GluI GCNGC 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 2 cut(s) 111, 259
HapII CCGG 2 cut(s) 60, 207
HhaI GCGC 1 cut(s) 146
Hin1II CATG 2 cut(s) 124, 326
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 5 cut(s) 136, 368, 404, 536, 545
HpaII CCGG 2 cut(s) 60, 207
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 3 cut(s) 130, 283, 408
Hpy188I TCNGA 1 cut(s) 541
Hpy8I GTNNAC 3 cut(s) 130, 283, 408
HpyAV CCTTC 4 cut(s) 178, 199, 337, 409
HpyCH4III ACNGT 4 cut(s) 67, 200, 379, 466
HpyCH4V TGCA 7 cut(s) 20, 117, 239, 322, 350, 398, 427
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 332, 433
HpyF3I CTNAG 2 cut(s) 140, 411
Hsp92II CATG 2 cut(s) 124, 326
HspAI GCGC 1 cut(s) 144
KpnI GGTACC 1 cut(s) 508
Kzo9I GATC 1 cut(s) 339
LmnI GCTCC 1 cut(s) 54
Lsp1109I GCAGC 1 cut(s) 411
MaeIII GTNAC 1 cut(s) 200
MalI GATC 1 cut(s) 341
MboI GATC 1 cut(s) 339
MboII GAAGA 1 cut(s) 519
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 4 cut(s) 234, 444, 498, 523
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 3 cut(s) 413, 530, 539
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 7 cut(s) 50, 62, 95, 271, 375, 447, 535
Mox20I TGGCCA 1 cut(s) 111
MscI TGGCCA 1 cut(s) 111
MseI TTAA 2 cut(s) 23, 98
Msp20I TGGCCA 1 cut(s) 111
MspI CCGG 2 cut(s) 60, 207
MwoI GCNNNNNNNGC 3 cut(s) 164, 332, 433
NdeII GATC 1 cut(s) 339
NlaIII CATG 2 cut(s) 124, 326
NlaIV GGNNCC 1 cut(s) 506
NspI RCATGY 2 cut(s) 124, 326
PaeI GCATGC 1 cut(s) 326
PciI ACATGT 1 cut(s) 120
PfeI GAWTC 2 cut(s) 136, 368
PkrI GCNGC 1 cut(s) 426
PleI GAGTC 3 cut(s) 412, 530, 539
PpsI GAGTC 3 cut(s) 412, 530, 539
PscI ACATGT 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 506
PspPI GGNCC 1 cut(s) 258
PstI CTGCAG 1 cut(s) 429
RsaI GTAC 2 cut(s) 463, 506
RsaNI GTAC 2 cut(s) 462, 505
SaqAI TTAA 2 cut(s) 23, 98
SatI GCNGC 1 cut(s) 425
Sau3AI GATC 1 cut(s) 339
Sau96I GGNCC 1 cut(s) 258
SchI GAGTC 3 cut(s) 413, 530, 539
SetI ASST 6 cut(s) 31, 42, 51, 303, 386, 420
SfcI CTRYAG 1 cut(s) 425
SphI GCATGC 1 cut(s) 326
Sse9I AATT 4 cut(s) 234, 444, 498, 523
SsiI CCGC 1 cut(s) 165
TaaI ACNGT 4 cut(s) 67, 200, 379, 466
TasI AATT 4 cut(s) 234, 444, 498, 523
TfiI GAWTC 2 cut(s) 136, 368
Tru1I TTAA 2 cut(s) 23, 98
Tru9I TTAA 2 cut(s) 23, 98
TscAI CASTG 4 cut(s) 217, 357, 382, 415
TseI GCWGC 1 cut(s) 424
TspDTI ATGAA 1 cut(s) 142
TspGWI ACGGA 2 cut(s) 115, 510
TspRI CASTG 4 cut(s) 217, 357, 382, 415
XceI RCATGY 2 cut(s) 124, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.