Rw4G025900

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016829
Physical Location & Seq
Reverse (-)
52035212 .. 52037049
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025900.1

Sequence Viewer

Length: 693 bp
ATGCAGCTTGTTGTGGATCTTCATAACTTGCTCGGAGGAGACTCGAATCAGACTAGTAAGGAGTTGAAGCGCCTGAGGGTTTTGAGGATGGCAGTGATTTACATGATGATGTTTGTGCTGAATCTGTACGAGTTCGCGAAGAAGAACTTGGGGCCTCTGAGATTGACTGTGTGGACTGTGGAGGTTGCTATGATCACTATTGTTGGTCCAGTCTACCAGAGATTCAAGGGAGTGCCTGATGATTTCTTTGCTTTACTCGACACGAAGATTGATGAAGCAGCTGACAAGTTTGATAAGCATGCTCCTCCTTTGGCTAAGCAGGTGGTGTACCTAAGGCTAGGTTCTATCCAGAAAGCATTGAAAAAGACCCGAGAATTTGTGAACGAGGCTCAGGCTGGCGGTCTACACACAGCTATTCATTCTGCAGCTACAGAATATAAGCTGCTTGTCTTCAACCAATCCATGAAGTTTTGGGTTGGACTCGAGTACCAGTGTCCTCCCATACACACAGTGGTAGCCTTCTCTACACCAGCTGCTGCTTACTTGTCCCGGAAATACAACAAGGTGGTAAGAGACTTGAACCTGAAGGGTTACACTATCTTTGGATATTTACCTTTGGTTCCGATCGATCAGATATCAACAGCACTTAATAGGCGAGGCAGAGAAGATAGGAGAGGCAGCTATGCATGTTGA

Protein Analysis

230

Amino Acids

26.1

Weight (kDa)

9.63

Isoelectric Point (pI)

29.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 17 - 217 5.1e-61 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016829)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23780
rosa_chinensis RchiOBHm_Chr4g0430271
rosa_laevigata RLG00000006989
rosa_multiflora Rmu_sc0001089.1_g000025 Rmu_sc0027338.1_g000002
rosa_roxburghii Rroxscaffold_5G00372070
rosa_rugosa Rorug04G0242200 Rorug04G0242300 Rorug04G0242400
rosa_samantha Rh4AG298700 Rh4BG305300 Rh4CG320800 Rh4DG302600
rosa_wichuraiana Rw4G025900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 310
Acc36I ACCTGC 1 cut(s) 310
AccI GTMKAC 2 cut(s) 213, 403
AccII CGCG 1 cut(s) 137
AciI CCGC 1 cut(s) 399
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 1 cut(s) 374
AcuI CTGAAG 1 cut(s) 605
AdeI CACNNNGTG 1 cut(s) 511
AfaI GTAC 3 cut(s) 128, 329, 488
AgsI TTSAA 5 cut(s) 67, 226, 361, 454, 580
AhlI ACTAGT 1 cut(s) 53
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 7 cut(s) 7, 281, 413, 428, 442, 533, 681
AluI AGCT 7 cut(s) 7, 281, 413, 428, 442, 533, 681
Alw26I GTCTC 2 cut(s) 33, 567
AlwI GGATC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 536
Ama87I CYCGRG 2 cut(s) 369, 482
AoxI GGCC 1 cut(s) 152
ApeKI GCWGC 7 cut(s) 4, 278, 425, 442, 533, 536, 678
ApoI RAATTY 1 cut(s) 374
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 2 cut(s) 152, 206
AsuC2I CCSGG 1 cut(s) 550
AvaI CYCGRG 2 cut(s) 369, 482
AvaII GGWCC 1 cut(s) 206
AxyI CCTNAGG 2 cut(s) 74, 332
BaeI ACNNNNGTAYC 2 cut(s) 470, 503
BbsI GAAGAC 1 cut(s) 442
BbvI GCAGC 6 cut(s) 16, 290, 429, 437, 520, 523
BccI CCATC 1 cut(s) 82
BclI TGATCA 1 cut(s) 192
BcnI CCSGG 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 33, 567
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 2 cut(s) 54, 338
BfmI CTRYAG 2 cut(s) 423, 429
BfoI RGCGCY 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 310
BisI GCNGC 7 cut(s) 5, 279, 426, 443, 534, 537, 679
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 7 cut(s) 6, 280, 427, 444, 535, 538, 680
Bme1390I CCNGG 1 cut(s) 550
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 2 cut(s) 369, 482
BmgT120I GGNCC 2 cut(s) 152, 206
BmiI GGNNCC 2 cut(s) 153, 621
BmrFI CCNGG 1 cut(s) 550
BpiI GAAGAC 1 cut(s) 442
Bpu10I CCTNAGC 1 cut(s) 390
Bpu1102I GCTNAGC 1 cut(s) 315
BpuMI CCSGG 1 cut(s) 550
Bsa29I ATCGAT 1 cut(s) 627
Bse1I ACTGG 2 cut(s) 209, 490
Bse21I CCTNAGG 2 cut(s) 74, 332
BseCI ATCGAT 1 cut(s) 627
BseGI GGATG 1 cut(s) 93
BseMII CTCAG 3 cut(s) 65, 149, 404
BseNI ACTGG 2 cut(s) 209, 490
BseRI GAGGAG 2 cut(s) 51, 294
BseXI GCAGC 6 cut(s) 16, 290, 429, 437, 520, 523
Bsh1236I CGCG 1 cut(s) 137
Bsh1285I CGRYCG 1 cut(s) 627
BshFI GGCC 1 cut(s) 154
BshVI ATCGAT 1 cut(s) 627
BsiEI CGRYCG 1 cut(s) 627
BsiHKCI CYCGRG 2 cut(s) 369, 482
BsiSI CCGG 1 cut(s) 550
BslFI GGGAC 1 cut(s) 532
BsmAI GTCTC 2 cut(s) 33, 567
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 1 cut(s) 154
BsoBI CYCGRG 2 cut(s) 369, 482
Bsp143I GATC 4 cut(s) 16, 192, 624, 628
Bsp1720I GCTNAGC 1 cut(s) 315
Bsp68I TCGCGA 1 cut(s) 137
BspACI CCGC 1 cut(s) 399
BspANI GGCC 1 cut(s) 154
BspCNI CTCAG 3 cut(s) 66, 150, 403
BspDI ATCGAT 1 cut(s) 627
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 2 cut(s) 153, 621
BspMAI CTGCAG 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 1 cut(s) 24
BsrI ACTGG 2 cut(s) 209, 490
BssMI GATC 4 cut(s) 16, 192, 624, 628
Bst4CI ACNGT 3 cut(s) 169, 178, 511
BstC8I GCNNGC 2 cut(s) 300, 397
BstDEI CTNAG 5 cut(s) 74, 158, 315, 332, 390
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 1 cut(s) 137
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 4 cut(s) 19, 195, 627, 631
BstMAI GTCTC 2 cut(s) 33, 567
BstMBI GATC 4 cut(s) 16, 192, 624, 628
BstMCI CGRYCG 1 cut(s) 627
BstNSI RCATGY 2 cut(s) 302, 690
BstSCI CCNGG 1 cut(s) 548
BstSFI CTRYAG 2 cut(s) 423, 429
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 6 cut(s) 16, 290, 429, 437, 520, 523
BstV2I GAAGAC 1 cut(s) 442
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 627
Bsu36I CCTNAGG 2 cut(s) 74, 332
BsuRI GGCC 1 cut(s) 154
BsuTUI ATCGAT 1 cut(s) 627
BtsCI GGATG 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 99, 497, 516
BtuMI TCGCGA 1 cut(s) 137
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 2 cut(s) 300, 397
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 2 cut(s) 152, 206
ClaI ATCGAT 1 cut(s) 627
Csp6I GTAC 3 cut(s) 127, 328, 487
CviAII CATG 4 cut(s) 103, 299, 463, 687
CviQI GTAC 3 cut(s) 127, 328, 487
DdeI CTNAG 5 cut(s) 74, 158, 315, 332, 390
DpnI GATC 4 cut(s) 18, 194, 626, 630
DpnII GATC 4 cut(s) 16, 192, 624, 628
DraIII CACNNNGTG 1 cut(s) 511
Eco32I GATATC 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 605
Eco81I CCTNAGG 2 cut(s) 74, 332
Eco88I CYCGRG 2 cut(s) 369, 482
EcoO109I RGGNCCY 1 cut(s) 152
EcoRV GATATC 1 cut(s) 636
EcoT22I ATGCAT 1 cut(s) 688
FaeI CATG 4 cut(s) 106, 302, 466, 690
FaiI YATR 9 cut(s) 24, 104, 191, 300, 438, 464, 503, 684, 688
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 1 cut(s) 532
FatI CATG 4 cut(s) 102, 298, 462, 686
FbaI TGATCA 1 cut(s) 192
FblI GTMKAC 2 cut(s) 213, 403
Fnu4HI GCNGC 7 cut(s) 5, 279, 426, 443, 534, 537, 679
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 7 cut(s) 5, 279, 426, 443, 534, 537, 679
FspBI CTAG 2 cut(s) 54, 338
GlaI GCGC 1 cut(s) 71
GluI GCNGC 7 cut(s) 5, 279, 426, 443, 534, 537, 679
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 1 cut(s) 154
HapII CCGG 1 cut(s) 550
HhaI GCGC 1 cut(s) 72
Hin1II CATG 4 cut(s) 106, 302, 466, 690
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HinfI GANTC 5 cut(s) 41, 46, 121, 222, 480
HpaII CCGG 1 cut(s) 550
Hpy166II GTNNAC 5 cut(s) 174, 214, 328, 382, 404
Hpy188I TCNGA 5 cut(s) 35, 51, 159, 624, 633
Hpy188III TCNNGA 2 cut(s) 136, 349
Hpy8I GTNNAC 5 cut(s) 174, 214, 328, 382, 404
HpyAV CCTTC 2 cut(s) 529, 580
HpyCH4III ACNGT 3 cut(s) 169, 178, 511
HpyCH4V TGCA 3 cut(s) 4, 425, 686
HpyF3I CTNAG 5 cut(s) 74, 158, 315, 332, 390
Hsp92II CATG 4 cut(s) 106, 302, 466, 690
HspAI GCGC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 4 cut(s) 16, 192, 624, 628
LmnI GCTCC 1 cut(s) 307
Lsp1109I GCAGC 6 cut(s) 16, 290, 429, 437, 520, 523
MaeI CTAG 2 cut(s) 54, 338
MaeIII GTNAC 1 cut(s) 590
MalI GATC 4 cut(s) 18, 194, 626, 630
MboI GATC 4 cut(s) 16, 192, 624, 628
MboII GAAGA 6 cut(s) 11, 151, 154, 277, 442, 677
MflI RGATCY 1 cut(s) 16
MluCI AATT 1 cut(s) 374
MlyI GAGTC 2 cut(s) 35, 474
MmeI TCCRAC 1 cut(s) 457
Mph1103I ATGCAT 1 cut(s) 688
MseI TTAA 1 cut(s) 648
MslI CAYNNNNRTG 1 cut(s) 107
MspA1I CMGCKG 2 cut(s) 281, 533
MspI CCGG 1 cut(s) 550
MspR9I CCNGG 1 cut(s) 550
MvnI CGCG 1 cut(s) 137
NciI CCSGG 1 cut(s) 550
NdeII GATC 4 cut(s) 16, 192, 624, 628
NlaIII CATG 4 cut(s) 106, 302, 466, 690
NlaIV GGNNCC 2 cut(s) 153, 621
NruI TCGCGA 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 2 cut(s) 302, 690
PaeI GCATGC 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 482
PaqCI CACCTGC 1 cut(s) 310
PfeI GAWTC 3 cut(s) 46, 121, 222
PfoI TCCNGGA 1 cut(s) 548
PkrI GCNGC 7 cut(s) 6, 280, 427, 444, 535, 538, 680
Ple19I CGATCG 1 cut(s) 627
PleI GAGTC 2 cut(s) 35, 474
PpsI GAGTC 2 cut(s) 35, 474
PspN4I GGNNCC 2 cut(s) 153, 621
PspPI GGNCC 2 cut(s) 152, 206
PspXI VCTCGAGB 1 cut(s) 482
PstI CTGCAG 1 cut(s) 427
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 1 cut(s) 16
PvuI CGATCG 1 cut(s) 627
PvuII CAGCTG 2 cut(s) 281, 533
RruI TCGCGA 1 cut(s) 137
RsaI GTAC 3 cut(s) 128, 329, 488
RsaNI GTAC 3 cut(s) 127, 328, 487
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 7 cut(s) 5, 279, 426, 443, 534, 537, 679
Sau3AI GATC 4 cut(s) 16, 192, 624, 628
Sau96I GGNCC 2 cut(s) 152, 206
SchI GAGTC 2 cut(s) 35, 474
ScrFI CCNGG 1 cut(s) 550
SfcI CTRYAG 2 cut(s) 423, 429
Sfr274I CTCGAG 1 cut(s) 482
SinI GGWCC 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 482
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SpeI ACTAGT 1 cut(s) 53
SphI GCATGC 1 cut(s) 302
Sse9I AATT 1 cut(s) 374
SsiI CCGC 1 cut(s) 399
SspMI CTAG 2 cut(s) 54, 338
StyD4I CCNGG 1 cut(s) 548
TaaI ACNGT 3 cut(s) 169, 178, 511
TaqI TCGA 4 cut(s) 44, 258, 483, 627
TasI AATT 1 cut(s) 374
TfiI GAWTC 3 cut(s) 46, 121, 222
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 3 cut(s) 99, 497, 516
TseI GCWGC 7 cut(s) 4, 278, 425, 442, 533, 536, 678
TspDTI ATGAA 4 cut(s) 11, 288, 407, 479
TspRI CASTG 3 cut(s) 99, 497, 516
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 1 cut(s) 374
XceI RCATGY 2 cut(s) 302, 690
XcmI CCANNNNNNNNNTGG 1 cut(s) 508
XhoI CTCGAG 1 cut(s) 482
XmiI GTMKAC 2 cut(s) 213, 403
XspI CTAG 2 cut(s) 54, 338
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.