Rh4BG305300

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016829
Physical Location & Seq
Reverse (-)
47547161 .. 47549381
2221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG305300.1

Sequence Viewer

Length: 411 bp
ATGGTTGTTGCAGAGAAATCGAACGAAGCTGAGATCACCGAGCAGGACGCGCTTCTTCAGGTGGTGTACCTGACTCTGGGTTCTATCCAGAAAGCATTGAAAAAGACCCAAGCATTTGTGAACGAGACTCAGGCTGGCGGTCTACACACAGCTATCCATTCTGCAGCTACAGAATATAAGCTGCTTCTCTTCAACCAATCCATGAAGTTTTGGGTTGGACTCGAGTACCAGTGTCCTCCCATACACACAGTGGCAGAGAAGGCTACACCAGCTGCTGCTTGCTTGTCCCGGAAATACAACAAAGTGGTAAGAGACTTGAACCTGAAGGGTTACACTATCTTTGGATATTTACCTTTGGTTCCGATCGATCAGATATCAACAGCATTTAATAAGGCGAGGCAGAGAAGATAG

Protein Analysis

136

Amino Acids

15.19

Weight (kDa)

9.27

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 26 - 130 1.5e-24 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016829)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23780
rosa_chinensis RchiOBHm_Chr4g0430271
rosa_laevigata RLG00000006989
rosa_multiflora Rmu_sc0001089.1_g000025 Rmu_sc0027338.1_g000002
rosa_roxburghii Rroxscaffold_5G00372070
rosa_rugosa Rorug04G0242200 Rorug04G0242300 Rorug04G0242400
rosa_samantha Rh4AG298700 Rh4BG305300 Rh4CG320800 Rh4DG302600
rosa_wichuraiana Rw4G025900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 142
AccII CGCG 1 cut(s) 50
AciI CCGC 1 cut(s) 138
AcuI CTGAAG 2 cut(s) 41, 344
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 2 cut(s) 68, 227
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 3 cut(s) 100, 193, 319
AluBI AGCT 5 cut(s) 29, 152, 167, 181, 272
AluI AGCT 5 cut(s) 29, 152, 167, 181, 272
Alw26I GTCTC 2 cut(s) 119, 306
AlwNI CAGNNNCTG 1 cut(s) 275
Ama87I CYCGRG 1 cut(s) 221
ApeKI GCWGC 4 cut(s) 164, 181, 272, 275
AspLEI GCGC 1 cut(s) 52
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 221
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BbvI GCAGC 4 cut(s) 168, 176, 259, 262
BcgI CGANNNNNNTGC 1 cut(s) 34
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 119, 306
BfmI CTRYAG 2 cut(s) 162, 168
BisI GCNGC 4 cut(s) 165, 182, 273, 276
BlsI GCNGC 4 cut(s) 166, 183, 274, 277
Bme1390I CCNGG 1 cut(s) 289
BmeT110I CYCGRG 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 1 cut(s) 289
BpuMI CCSGG 1 cut(s) 289
Bsa29I ATCGAT 1 cut(s) 366
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 229
BseCI ATCGAT 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 2 cut(s) 21, 143
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 4 cut(s) 168, 176, 259, 262
Bsh1236I CGCG 1 cut(s) 50
Bsh1285I CGRYCG 1 cut(s) 366
BshVI ATCGAT 1 cut(s) 366
BsiEI CGRYCG 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 1 cut(s) 289
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 2 cut(s) 119, 306
BsmFI GGGAC 1 cut(s) 271
BsoBI CYCGRG 1 cut(s) 221
Bsp143I GATC 3 cut(s) 33, 363, 367
BspACI CCGC 1 cut(s) 138
BspCNI CTCAG 2 cut(s) 22, 142
BspDI ATCGAT 1 cut(s) 366
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 1 cut(s) 360
BspMAI CTGCAG 1 cut(s) 166
BsrI ACTGG 1 cut(s) 229
BssMI GATC 3 cut(s) 33, 363, 367
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 136, 280
BstDEI CTNAG 2 cut(s) 30, 129
BstFNI CGCG 1 cut(s) 50
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 3 cut(s) 36, 366, 370
BstMAI GTCTC 2 cut(s) 119, 306
BstMBI GATC 3 cut(s) 33, 363, 367
BstMCI CGRYCG 1 cut(s) 366
BstMWI GCNNNNNNNGC 3 cut(s) 49, 260, 269
BstSCI CCNGG 1 cut(s) 287
BstSFI CTRYAG 2 cut(s) 162, 168
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 4 cut(s) 168, 176, 259, 262
Bsu15I ATCGAT 1 cut(s) 366
BsuTUI ATCGAT 1 cut(s) 366
BtsIMutI CAGTG 2 cut(s) 236, 255
Cac8I GCNNGC 2 cut(s) 136, 280
CaiI CAGNNNCTG 1 cut(s) 275
CfoI GCGC 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 366
CseI GACGC 1 cut(s) 56
Csp6I GTAC 2 cut(s) 67, 226
CviAII CATG 1 cut(s) 202
CviJI RGCY 7 cut(s) 29, 134, 152, 167, 181, 263, 272
CviKI_1 RGCY 7 cut(s) 29, 134, 152, 167, 181, 263, 272
CviQI GTAC 2 cut(s) 67, 226
DdeI CTNAG 2 cut(s) 30, 129
DpnI GATC 3 cut(s) 35, 365, 369
DpnII GATC 3 cut(s) 33, 363, 367
DraIII CACNNNGTG 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco32I GATATC 1 cut(s) 375
Eco57I CTGAAG 2 cut(s) 41, 344
Eco88I CYCGRG 1 cut(s) 221
EcoRV GATATC 1 cut(s) 375
FaeI CATG 1 cut(s) 205
FaiI YATR 3 cut(s) 177, 203, 242
FaqI GGGAC 1 cut(s) 271
FatI CATG 1 cut(s) 201
FblI GTMKAC 1 cut(s) 142
Fnu4HI GCNGC 4 cut(s) 165, 182, 273, 276
Fsp4HI GCNGC 4 cut(s) 165, 182, 273, 276
GlaI GCGC 1 cut(s) 51
GluI GCNGC 4 cut(s) 165, 182, 273, 276
HapII CCGG 1 cut(s) 289
HgaI GACGC 1 cut(s) 56
HhaI GCGC 1 cut(s) 52
Hin1II CATG 1 cut(s) 205
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HinfI GANTC 3 cut(s) 73, 127, 219
HpaII CCGG 1 cut(s) 289
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 3 cut(s) 67, 121, 143
Hpy188I TCNGA 2 cut(s) 363, 372
Hpy188III TCNNGA 1 cut(s) 88
Hpy8I GTNNAC 3 cut(s) 67, 121, 143
HpyAV CCTTC 2 cut(s) 253, 319
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 2 cut(s) 11, 164
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 260, 269
HpyF3I CTNAG 2 cut(s) 30, 129
Hsp92II CATG 1 cut(s) 205
HspAI GCGC 1 cut(s) 50
Kzo9I GATC 3 cut(s) 33, 363, 367
Lsp1109I GCAGC 4 cut(s) 168, 176, 259, 262
MaeIII GTNAC 1 cut(s) 329
MalI GATC 3 cut(s) 35, 365, 369
MboI GATC 3 cut(s) 33, 363, 367
MboII GAAGA 2 cut(s) 47, 181
MlyI GAGTC 3 cut(s) 67, 121, 213
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 2 cut(s) 246, 390
MseI TTAA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 1 cut(s) 289
MspR9I CCNGG 1 cut(s) 289
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 3 cut(s) 49, 260, 269
NciI CCSGG 1 cut(s) 289
NdeII GATC 3 cut(s) 33, 363, 367
NlaIII CATG 1 cut(s) 205
NlaIV GGNNCC 1 cut(s) 360
PaeR7I CTCGAG 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 287
PkrI GCNGC 4 cut(s) 166, 183, 274, 277
Ple19I CGATCG 1 cut(s) 366
PleI GAGTC 3 cut(s) 67, 121, 213
PpsI GAGTC 3 cut(s) 67, 121, 213
PspN4I GGNNCC 1 cut(s) 360
PspXI VCTCGAGB 1 cut(s) 221
PstI CTGCAG 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 275
PvuI CGATCG 1 cut(s) 366
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 2 cut(s) 68, 227
RsaNI GTAC 2 cut(s) 67, 226
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 4 cut(s) 165, 182, 273, 276
Sau3AI GATC 3 cut(s) 33, 363, 367
SchI GAGTC 3 cut(s) 67, 121, 213
ScrFI CCNGG 1 cut(s) 289
SetI ASST 9 cut(s) 31, 63, 72, 154, 169, 183, 274, 324, 355
SfcI CTRYAG 2 cut(s) 162, 168
Sfr274I CTCGAG 1 cut(s) 221
SlaI CTCGAG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
SsiI CCGC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 287
TaaI ACNGT 1 cut(s) 250
TaqI TCGA 3 cut(s) 20, 222, 366
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 2 cut(s) 236, 255
TseI GCWGC 4 cut(s) 164, 181, 272, 275
TspDTI ATGAA 1 cut(s) 218
TspRI CASTG 2 cut(s) 236, 255
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XhoI CTCGAG 1 cut(s) 221
XmiI GTMKAC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.