Rorug04G0242400

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011256
Physical Location & Seq
Forward (+)
41111341 .. 41113816
2476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0242400.1

Sequence Viewer

Length: 1338 bp
ATGAATCCACAGGAAATAGTACCGGCCAGAAGCAAAGAACAAGGAGAAGAATGGGAGCCCAAAGCTCCTTCCAACTTCAGGAAACTCCCACATGCTTTAGTCATGGACATTCTCTCAAGGCTCACAGTCATCACGCTCTTCAGTTGCAGGTGCGTTTGCAAATCATGGCTCTCTCTTATTTCCGACCCCAAATTTACTCATCTTCACCGTTCGAGATCATCCTTTGGCATCTTGATGCAAAACATACCGTTTTTTGAGAAATCAAAGGAAGCTTATTTCGCCCAGATACTAGAATGTGGAGGTTCTGATGCTTTCGTTGAGGAAATAATATTTACCCCACAAAATAGCATACCTGTCTCTGAATTTGGCTTGGTAAACTCATGCAATGGTTTGCTTCTTTTGTCTGGAGCTAAAAGTGATGACCCTTTGTATGTATGCAATCCAATTTTGGGTGAATACATCACCATTCCACCTACTAAGGGTAGGCGTTGGGGTAGTTTCATAGGACTTGGTTTTAGCGTCACGACCGATGAGTACAAGGTGTTGCAAACATTTTGTCCAGTGAACGAGTTGTGCACTTGCTCACATAATTACTACGAAGCTAAGATCTACACCATTGGTACAGGAGTTTGGAGAAGCATTGGAAATGTTCCTAGAGAACTTGTTGTGTTGCCCTTTAATAGTTTTCTCCATGGAGCTCTTCATTGGGTGCCATTTGAGGGTTATAGTTCTGAGTTTATATATTCATTCAACTTTGAAAGAGAAAAGTTTCAACCACTACCCCCACCTAGCTACTTTGGACCATTTGAGAAGGAATATTCACATTGTTTGAAGTTGGGAGTGTTAGAAGGTTCTCTTGTTTTGAGTGCCTTTGGTGCTGATACTAGCAAATTCGACTTGTGGATTATGAAGGATTATGGGGTCCAAGAGTCTTGGACTAAAATTCTTGTCATTGAAAATTTAAATCTGCATCTTAGCAACTCTGGCTGTGAATTGCTGGAGCCACTTATGTTTCTGCGTAATGGGGACATCCTAATGACGGTAGATGATCGTTATGTAGCCTTTTATGATCAAAAGAGAAAGAGGTTCTGCGAAGCTATAATGTCTCAAACAGAGTGGAAATTTCATTCAGTTGCTTACAGTCCATGCTTTGTCTCGCTTTATGATGTTTCAAAAGGAGAGCAGGTGAAAAGAGTAAGAGGAGGTAAGAGATCTGAGAAGCTGTTTGGCGAAGGGAGCAGTGGTTGTGCTGGTTCTAGATCATCTCCAGTCGCAACCACAAGTCAGCAAAACATAACTCTGGCTATGGATGACTTGTGTCTGAAGATGGTCGACTAA

Protein Analysis

445

Amino Acids

50.29

Weight (kDa)

6.05

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 27 - 64 2.1e-08 F-box-like
F-box PF00646 28 - 67 1.6e-09 F-box domain
b-prop_At3g26010-like PF24750 116 - 240 1.4e-06 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 119 - 371 8.3e-26 F-box associated beta propeller domain
FBA_3 PF08268 122 - 372 9.2e-21 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67360 AT1G67360
fragaria_vesca FvH4_4g23790
malus_domestica MD01G1161800.v1.1 MD08G1165600.v1.1 MD13G1118800.v1.1 MD16G1119000.v1.1
prunus_persica Prupe.1G226000_v2.0.a1
pyrus_communis pycom13g10370 pycom16g10190
rosa_chinensis RchiOBHm_Chr4g0430281
rosa_laevigata RLG00000006988
rosa_multiflora Rmu_sc0001089.1_g000026
rosa_roxburghii Rroxscaffold_5G00372080
rosa_rugosa Rorug04G0242400
rosa_samantha Rh4AG298800 Rh4BG305400 Rh4CG320900 Rh4DG302700
rosa_wichuraiana Rw4G025910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 138, 1174
Acc36I ACCTGC 2 cut(s) 138, 1174
AccB1I GGYRCC 1 cut(s) 709
AccI GTMKAC 1 cut(s) 1332
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 6 cut(s) 191, 362, 890, 942, 958, 1121
AcuI CTGAAG 2 cut(s) 61, 124
AfaI GTAC 3 cut(s) 21, 536, 622
AfiI CCNNNNNNNGG 5 cut(s) 78, 449, 479, 719, 1039
AgsI TTSAA 6 cut(s) 751, 758, 773, 832, 956, 1173
AluBI AGCT 8 cut(s) 65, 272, 410, 602, 698, 792, 1097, 1222
AluI AGCT 8 cut(s) 65, 272, 410, 602, 698, 792, 1097, 1222
Alw21I GWGCWC 2 cut(s) 578, 700
Alw26I GTCTC 3 cut(s) 361, 1110, 1159
Alw44I GTGCAC 1 cut(s) 574
AoxI GGCC 1 cut(s) 24
ApaLI GTGCAC 1 cut(s) 574
ApoI RAATTY 6 cut(s) 191, 362, 890, 942, 958, 1121
ArsI GACNNNNNNTTYG 2 cut(s) 541, 573
Asp700I GAANNNNTTC 1 cut(s) 768
AspS9I GGNCC 2 cut(s) 800, 922
AsuHPI GGTGA 4 cut(s) 197, 454, 464, 1198
AvaII GGWCC 2 cut(s) 800, 922
BaeGI GKGCMC 1 cut(s) 578
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
BanI GGYRCC 1 cut(s) 709
BanII GRGCYC 2 cut(s) 60, 700
Bbv12I GWGCWC 2 cut(s) 578, 700
BccI CCATC 1 cut(s) 1321
BclI TGATCA 1 cut(s) 1069
BcoDI GTCTC 3 cut(s) 361, 1110, 1159
BfaI CTAG 5 cut(s) 290, 654, 789, 885, 1257
BfuAI ACCTGC 2 cut(s) 138, 1174
BglII AGATCT 2 cut(s) 606, 1211
Bme18I GGWCC 2 cut(s) 800, 922
BmgT120I GGNCC 2 cut(s) 800, 922
BmiI GGNNCC 4 cut(s) 57, 711, 923, 1002
BmsI GCATC 4 cut(s) 225, 237, 298, 979
BoxI GACNNNNGTC 1 cut(s) 1317
BpmI CTGGAG 3 cut(s) 426, 1019, 1251
BpuEI CTTGAG 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 691
BsaXI ACNNNNNCTCC 4 cut(s) 1170, 1200, 1228, 1258
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 449, 479, 719, 1039
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 2 cut(s) 560, 1268
Bse3DI GCAATG 1 cut(s) 391
BseDI CCNNGG 1 cut(s) 691
BseGI GGATG 3 cut(s) 218, 1029, 1315
BseLI CCNNNNNNNGG 5 cut(s) 78, 449, 479, 719, 1039
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 2 cut(s) 723, 1206
BseNI ACTGG 2 cut(s) 560, 1268
BseRI GAGGAG 1 cut(s) 1215
BseSI GKGCMC 1 cut(s) 578
Bsh1285I CGRYCG 1 cut(s) 528
BshFI GGCC 1 cut(s) 26
BshNI GGYRCC 1 cut(s) 709
BsiEI CGRYCG 1 cut(s) 528
BsiHKAI GWGCWC 2 cut(s) 578, 700
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 1040
BslI CCNNNNNNNGG 5 cut(s) 78, 449, 479, 719, 1039
BsmAI GTCTC 3 cut(s) 361, 1110, 1159
BsmFI GGGAC 1 cut(s) 1040
BsnI GGCC 1 cut(s) 26
Bsp1286I GDGCHC 3 cut(s) 60, 578, 700
Bsp143I GATC 6 cut(s) 215, 606, 1048, 1069, 1211, 1259
Bsp19I CCATGG 1 cut(s) 691
BspANI GGCC 1 cut(s) 26
BspCNI CTCAG 2 cut(s) 724, 1207
BspLI GGNNCC 4 cut(s) 57, 711, 923, 1002
BspMI ACCTGC 2 cut(s) 138, 1174
BspQI GCTCTTC 2 cut(s) 143, 705
BspT107I GGYRCC 1 cut(s) 709
BsrDI GCAATG 1 cut(s) 391
BsrFI RCCGGY 1 cut(s) 22
BsrI ACTGG 2 cut(s) 560, 1268
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 691
BssMI GATC 6 cut(s) 215, 606, 1048, 1069, 1211, 1259
BssT1I CCWWGG 1 cut(s) 691
Bst4CI ACNGT 5 cut(s) 127, 209, 249, 1042, 1142
Bst6I CTCTTC 2 cut(s) 143, 705
BstDEI CTNAG 5 cut(s) 477, 603, 732, 974, 1215
BstDSI CCRYGG 1 cut(s) 691
BstF5I GGATG 3 cut(s) 218, 1029, 1315
BstKTI GATC 6 cut(s) 218, 609, 1051, 1072, 1214, 1262
BstMAI GTCTC 3 cut(s) 361, 1110, 1159
BstMBI GATC 6 cut(s) 215, 606, 1048, 1069, 1211, 1259
BstMCI CGRYCG 1 cut(s) 528
BstMWI GCNNNNNNNGC 4 cut(s) 278, 875, 984, 1236
BstNSI RCATGY 1 cut(s) 95
BstPAI GACNNNNGTC 1 cut(s) 1317
BstSLI GKGCMC 1 cut(s) 578
BstX2I RGATCY 2 cut(s) 606, 1211
BstYI RGATCY 2 cut(s) 606, 1211
BsuRI GGCC 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 691
BtsCI GGATG 3 cut(s) 218, 1029, 1315
BtsI GCAGTG 1 cut(s) 1246
BtsIMutI CAGTG 2 cut(s) 567, 1246
BveI ACCTGC 2 cut(s) 138, 1174
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 2 cut(s) 800, 922
CseI GACGC 1 cut(s) 508
Csp6I GTAC 3 cut(s) 20, 535, 621
CspCI CAANNNNNGTGG 2 cut(s) 1097, 1132
CviAII CATG 6 cut(s) 92, 103, 165, 381, 692, 1146
CviQI GTAC 3 cut(s) 20, 535, 621
DdeI CTNAG 5 cut(s) 477, 603, 732, 974, 1215
DpnI GATC 6 cut(s) 217, 608, 1050, 1071, 1213, 1261
DpnII GATC 6 cut(s) 215, 606, 1048, 1069, 1211, 1259
DraI TTTAAA 1 cut(s) 963
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 2 cut(s) 143, 705
EarI CTCTTC 2 cut(s) 143, 705
Ecl136II GAGCTC 1 cut(s) 698
Eco130I CCWWGG 1 cut(s) 691
Eco24I GRGCYC 2 cut(s) 60, 700
Eco47I GGWCC 2 cut(s) 800, 922
Eco53kI GAGCTC 1 cut(s) 698
Eco57I CTGAAG 2 cut(s) 61, 124
EcoICRI GAGCTC 1 cut(s) 698
EcoT14I CCWWGG 1 cut(s) 691
EcoT38I GRGCYC 2 cut(s) 60, 700
ErhI CCWWGG 1 cut(s) 691
FaeI CATG 6 cut(s) 95, 106, 168, 384, 695, 1149
FalI AAGNNNNNCTT 2 cut(s) 840, 872
FaqI GGGAC 1 cut(s) 1040
FatI CATG 6 cut(s) 91, 102, 164, 380, 691, 1145
FbaI TGATCA 1 cut(s) 1069
FblI GTMKAC 1 cut(s) 1332
FokI GGATG 3 cut(s) 205, 1016, 1322
FriOI GRGCYC 2 cut(s) 60, 700
FspBI CTAG 5 cut(s) 290, 654, 789, 885, 1257
GsuI CTGGAG 3 cut(s) 426, 1019, 1251
HaeIII GGCC 1 cut(s) 26
HapII CCGG 1 cut(s) 23
HgaI GACGC 1 cut(s) 508
Hin1II CATG 6 cut(s) 95, 106, 168, 384, 695, 1149
HincII GTYRAC 1 cut(s) 1333
HindII GTYRAC 1 cut(s) 1333
HindIII AAGCTT 1 cut(s) 270
HinfI GANTC 2 cut(s) 4, 929
HpaII CCGG 1 cut(s) 23
HphI GGTGA 4 cut(s) 197, 454, 464, 1198
Hpy166II GTNNAC 4 cut(s) 376, 565, 576, 1333
Hpy188I TCNGA 6 cut(s) 184, 307, 361, 733, 1216, 1323
Hpy188III TCNNGA 6 cut(s) 79, 213, 232, 405, 523, 1257
Hpy8I GTNNAC 4 cut(s) 376, 565, 576, 1333
HpyAV CCTTC 5 cut(s) 78, 805, 842, 904, 1226
HpyCH4III ACNGT 5 cut(s) 127, 209, 249, 1042, 1142
HpyCH4V TGCA 8 cut(s) 147, 159, 238, 384, 438, 547, 576, 970
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 875, 984, 1236
HpyF3I CTNAG 5 cut(s) 477, 603, 732, 974, 1215
Hsp92II CATG 6 cut(s) 95, 106, 168, 384, 695, 1149
Ksp22I TGATCA 1 cut(s) 1069
Kzo9I GATC 6 cut(s) 215, 606, 1048, 1069, 1211, 1259
LguI GCTCTTC 2 cut(s) 143, 705
LmnI GCTCC 6 cut(s) 55, 70, 407, 695, 1000, 1236
LweI GCATC 4 cut(s) 225, 237, 298, 979
MaeI CTAG 5 cut(s) 290, 654, 789, 885, 1257
MaeIII GTNAC 1 cut(s) 520
MalI GATC 6 cut(s) 217, 608, 1050, 1071, 1213, 1261
MboI GATC 6 cut(s) 215, 606, 1048, 1069, 1211, 1259
MboII GAAGA 5 cut(s) 59, 130, 194, 692, 1336
MflI RGATCY 2 cut(s) 606, 1211
MhlI GDGCHC 3 cut(s) 60, 578, 700
MluCI AATT 9 cut(s) 191, 362, 444, 589, 890, 942, 958, 992, 1121
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 2 cut(s) 96, 207
MnlI CCTC 6 cut(s) 293, 313, 712, 1077, 1193, 1196
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 2 cut(s) 678, 962
MslI CAYNNNNRTG 2 cut(s) 233, 1034
MspI CCGG 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 278, 875, 984, 1236
NcoI CCATGG 1 cut(s) 691
NdeII GATC 6 cut(s) 215, 606, 1048, 1069, 1211, 1259
NlaIII CATG 6 cut(s) 95, 106, 168, 384, 695, 1149
NlaIV GGNNCC 4 cut(s) 57, 711, 923, 1002
NmuCI GTSAC 1 cut(s) 520
NspI RCATGY 1 cut(s) 95
PaqCI CACCTGC 2 cut(s) 138, 1174
PciSI GCTCTTC 2 cut(s) 143, 705
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 1 cut(s) 4
PleI GAGTC 1 cut(s) 937
PpsI GAGTC 1 cut(s) 937
PshAI GACNNNNGTC 1 cut(s) 1317
Psp124BI GAGCTC 1 cut(s) 700
PspN4I GGNNCC 4 cut(s) 57, 711, 923, 1002
PspPI GGNCC 2 cut(s) 800, 922
PsuI RGATCY 2 cut(s) 606, 1211
RsaI GTAC 3 cut(s) 21, 536, 622
RsaNI GTAC 3 cut(s) 20, 535, 621
RseI CAYNNNNRTG 2 cut(s) 233, 1034
SacI GAGCTC 1 cut(s) 700
SalI GTCGAC 1 cut(s) 1331
SapI GCTCTTC 2 cut(s) 143, 705
SaqAI TTAA 2 cut(s) 678, 962
Sau3AI GATC 6 cut(s) 215, 606, 1048, 1069, 1211, 1259
Sau96I GGNCC 2 cut(s) 800, 922
SchI GAGTC 1 cut(s) 938
SduI GDGCHC 3 cut(s) 60, 578, 700
SfaNI GCATC 4 cut(s) 225, 237, 298, 979
SinI GGWCC 2 cut(s) 800, 922
SmiI ATTTAAAT 1 cut(s) 963
SmiMI CAYNNNNRTG 2 cut(s) 233, 1034
SmlI CTYRAG 1 cut(s) 115
SmoI CTYRAG 1 cut(s) 115
Sse9I AATT 9 cut(s) 191, 362, 444, 589, 890, 942, 958, 992, 1121
SspI AATATT 2 cut(s) 330, 818
SspMI CTAG 5 cut(s) 290, 654, 789, 885, 1257
SstI GAGCTC 1 cut(s) 700
StyI CCWWGG 1 cut(s) 691
SwaI ATTTAAAT 1 cut(s) 963
TaaI ACNGT 5 cut(s) 127, 209, 249, 1042, 1142
TaqI TCGA 3 cut(s) 212, 894, 1332
TaqII GACCGA 1 cut(s) 542
TasI AATT 9 cut(s) 191, 362, 444, 589, 890, 942, 958, 992, 1121
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 678, 962
Tru9I TTAA 2 cut(s) 678, 962
TscAI CASTG 2 cut(s) 567, 1246
TseFI GTSAC 1 cut(s) 520
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 6 cut(s) 17, 490, 692, 735, 923, 1115
TspRI CASTG 2 cut(s) 567, 1246
VneI GTGCAC 1 cut(s) 574
VpaK11BI GGWCC 2 cut(s) 800, 922
XapI RAATTY 6 cut(s) 191, 362, 890, 942, 958, 1121
XbaI TCTAGA 1 cut(s) 1256
XceI RCATGY 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 1332
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 5 cut(s) 290, 654, 789, 885, 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.