Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011256

Physical Location & Seq

Genomic Coordinates Reverse (-)

26697257 .. 26697919

Gene Structure 663 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1161800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 663 bp

ATGGCCACCGTCGATAAGGGAGTAGAGATCGGCAAGGAGAAGGAACTGAAGCACCTAGGGTTTCTGAGGGTGGCGGCGATTCAGACCCTGGTGTGCATTTCGAATCTGTACGATTACGCCAAGCAGAACTCCGGGCCGCTCAGATCGATGGTCGGGACGGTAGAGGGAGCGGTCACCGCCGTCGTTGGTCCCGTTTACCGGAAGTTTAAGGGCGTTCCTGACGATGTCCTCACTTTCCTCGACGCCAAGGTGGATGAAGCCACAGACAAGTTCGACAAGCATGCTCCTGTGGCCAAGCAGGTTGCAAGCCAAGCGCACTGCTTGATCCAGAAAGCAGCGGAAAAGGGGCAGAGAATTGTAAGGGAGGCTGAAACTGGTGGTCCACGCGCAGCTATTCACTATGCAGCTACTGAGTACAAGAACTTTGTCTTGAACCAATCTGTGAAGTTGTGGGTCGGACTCAACAAGTACCCTTCCATCCACACAGTGGCACAGAAGGCTGCACCAACCGCTGCGCACTGGTCTGGCAAATACAACCACATCGTAAAGGACTTGACCCGTAAGGGCTATGCCATCTTCGGCTATCTACCCCTGGTTCCGATCGATGAGATGTCTAAGGCAGTTGAAAAGGGAGAGACGGAGGCTGATTTTATCGTCAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005811 GO:0006996 GO:0007275 GO:0008150 GO:0009555 GO:0009987 GO:0012505 GO:0016043 GO:0032501 GO:0032502 GO:0034389 GO:0040007 GO:0040008 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044444 GO:0044464 GO:0045927 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048589 GO:0048856 GO:0050789 GO:0065007 GO:0071840 GO:0080134 GO:0080186 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

REF

Protein Analysis

221

Amino Acids

24.07

Weight (kDa)

9.03

Isoelectric Point (pI)

27.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
REF	PF05755	12 - 209	2.7e-82	Rubber elongation factor protein (REF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011256)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67360 AT1G67360
fragaria_vesca	FvH4_4g23790
malus_domestica	MD01G1161800.v1.1 MD08G1165600.v1.1 MD13G1118800.v1.1 MD16G1119000.v1.1
prunus_persica	Prupe.1G226000_v2.0.a1
pyrus_communis	pycom13g10370 pycom16g10190
rosa_chinensis	RchiOBHm_Chr4g0430281
rosa_laevigata	RLG00000006988
rosa_multiflora	Rmu_sc0001089.1_g000026
rosa_roxburghii	Rroxscaffold_5G00372080
rosa_rugosa	Rorug04G0242400
rosa_samantha	Rh4AG298800 Rh4BG305400 Rh4CG320900 Rh4DG302700
rosa_wichuraiana	Rw4G025910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	516
Acc36I	ACCTGC	1 cut(s)	289
AccB7I	CCANNNNNTGG	1 cut(s)	487
AccBSI	CCGCTC	2 cut(s)	139, 170
AccII	CGCG	1 cut(s)	387
AciI	CCGC	6 cut(s)	74, 137, 170, 177, 338, 510
AclWI	GGATC	1 cut(s)	319
AcoI	YGGCCR	2 cut(s)	3, 291
AcuI	CTGAAG	1 cut(s)	68
AcyI	GRCGYC	1 cut(s)	243
AdeI	CACNNNGTG	1 cut(s)	487
AfaI	GTAC	3 cut(s)	110, 416, 470
AfiI	CCNNNNNNNGG	2 cut(s)	198, 487
AgsI	TTSAA	2 cut(s)	433, 626
AjnI	CCWGG	2 cut(s)	87, 591
AluBI	AGCT	2 cut(s)	392, 407
AluI	AGCT	2 cut(s)	392, 407
Alw26I	GTCTC	1 cut(s)	629
AlwI	GGATC	1 cut(s)	319
AlwNI	CAGNNNCTG	2 cut(s)	88, 410
AoxI	GGCC	3 cut(s)	3, 134, 291
ApeKI	GCWGC	5 cut(s)	335, 389, 404, 500, 512
AspA2I	CCTAGG	1 cut(s)	55
AspLEI	GCGC	3 cut(s)	316, 389, 517
AspS9I	GGNCC	3 cut(s)	134, 188, 380
AsuC2I	CCSGG	1 cut(s)	133
AsuHPI	GGTGA	1 cut(s)	166
AsuII	TTCGAA	1 cut(s)	101
AvaII	GGWCC	2 cut(s)	188, 380
AvrII	CCTAGG	1 cut(s)	55
BalI	TGGCCA	2 cut(s)	5, 293
BbvI	GCAGC	5 cut(s)	347, 401, 416, 487, 499
BccI	CCATC	3 cut(s)	142, 485, 581
BceAI	ACGGC	1 cut(s)	164
BcgI	CGANNNNNNTGC	2 cut(s)	263, 297
BciT130I	CCWGG	2 cut(s)	89, 593
BcnI	CCSGG	1 cut(s)	133
BcoDI	GTCTC	1 cut(s)	629
BfaI	CTAG	1 cut(s)	56
BfuAI	ACCTGC	1 cut(s)	289
BisI	GCNGC	7 cut(s)	75, 137, 336, 390, 405, 501, 513
BlnI	CCTAGG	1 cut(s)	55
BlsI	GCNGC	7 cut(s)	76, 138, 337, 391, 406, 502, 514
Bme1390I	CCNGG	3 cut(s)	89, 133, 593
Bme18I	GGWCC	2 cut(s)	188, 380
BmgT120I	GGNCC	3 cut(s)	134, 188, 380
BmiI	GGNNCC	2 cut(s)	190, 597
BmrFI	CCNGG	3 cut(s)	89, 133, 593
Bpu14I	TTCGAA	1 cut(s)	101
BpuMI	CCSGG	1 cut(s)	133
Bsa29I	ATCGAT	2 cut(s)	146, 603
BsaHI	GRCGYC	1 cut(s)	243
BsaJI	CCNNGG	4 cut(s)	55, 87, 246, 591
BsaWI	WCCGGW	1 cut(s)	198
Bsc4I	CCNNNNNNNGG	2 cut(s)	198, 487
Bse1I	ACTGG	2 cut(s)	379, 524
BseBI	CCWGG	2 cut(s)	89, 593
BseCI	ATCGAT	2 cut(s)	146, 603
BseDI	CCNNGG	4 cut(s)	55, 87, 246, 591
BseGI	GGATG	2 cut(s)	259, 477
BseLI	CCNNNNNNNGG	2 cut(s)	198, 487
BseMII	CTCAG	3 cut(s)	56, 154, 402
BseNI	ACTGG	2 cut(s)	379, 524
BseXI	GCAGC	5 cut(s)	347, 401, 416, 487, 499
BsgI	GTGCAG	1 cut(s)	486
Bsh1236I	CGCG	1 cut(s)	387
Bsh1285I	CGRYCG	1 cut(s)	603
BshFI	GGCC	3 cut(s)	5, 136, 293
BshVI	ATCGAT	2 cut(s)	146, 603
BsiEI	CGRYCG	1 cut(s)	603
BsiSI	CCGG	2 cut(s)	132, 199
BslFI	GGGAC	2 cut(s)	169, 174
BslI	CCNNNNNNNGG	2 cut(s)	198, 487
BsmAI	GTCTC	1 cut(s)	629
BsmBI	CGTCTC	1 cut(s)	629
BsmFI	GGGAC	2 cut(s)	169, 174
BsnI	GGCC	3 cut(s)	5, 136, 293
Bsp119I	TTCGAA	1 cut(s)	101
Bsp143I	GATC	4 cut(s)	27, 143, 324, 600
BspACI	CCGC	6 cut(s)	74, 137, 170, 177, 338, 510
BspANI	GGCC	3 cut(s)	5, 136, 293
BspCNI	CTCAG	3 cut(s)	57, 153, 403
BspDI	ATCGAT	2 cut(s)	146, 603
BspFNI	CGCG	1 cut(s)	387
BspLI	GGNNCC	2 cut(s)	190, 597
BspMI	ACCTGC	1 cut(s)	289
BspPI	GGATC	1 cut(s)	319
BspT104I	TTCGAA	1 cut(s)	101
BsrBI	CCGCTC	2 cut(s)	139, 170
BsrI	ACTGG	2 cut(s)	379, 524
BssECI	CCNNGG	4 cut(s)	55, 87, 246, 591
BssMI	GATC	4 cut(s)	27, 143, 324, 600
BssNI	GRCGYC	1 cut(s)	243
BssT1I	CCWWGG	2 cut(s)	55, 246
Bst2UI	CCWGG	2 cut(s)	89, 593
Bst4CI	ACNGT	3 cut(s)	10, 160, 487
BstACI	GRCGYC	1 cut(s)	243
BstBI	TTCGAA	1 cut(s)	101
BstC8I	GCNNGC	2 cut(s)	282, 307
BstDEI	CTNAG	4 cut(s)	65, 140, 411, 615
BstEII	GGTNACC	1 cut(s)	172
BstF5I	GGATG	2 cut(s)	259, 477
BstFNI	CGCG	1 cut(s)	387
BstHHI	GCGC	3 cut(s)	316, 389, 517
BstKTI	GATC	4 cut(s)	30, 146, 327, 603
BstMAI	GTCTC	1 cut(s)	629
BstMBI	GATC	4 cut(s)	27, 143, 324, 600
BstMCI	CGRYCG	1 cut(s)	603
BstMWI	GCNNNNNNNGC	5 cut(s)	176, 290, 311, 497, 509
BstNI	CCWGG	2 cut(s)	89, 593
BstNSI	RCATGY	1 cut(s)	284
BstPI	GGTNACC	1 cut(s)	172
BstSCI	CCNGG	3 cut(s)	87, 131, 591
BstUI	CGCG	1 cut(s)	387
BstV1I	GCAGC	5 cut(s)	347, 401, 416, 487, 499
Bsu15I	ATCGAT	2 cut(s)	146, 603
BsuRI	GGCC	3 cut(s)	5, 136, 293
BsuTUI	ATCGAT	2 cut(s)	146, 603
BtsCI	GGATG	2 cut(s)	259, 477
BtsI	GCAGTG	1 cut(s)	316
BtsIMutI	CAGTG	3 cut(s)	316, 492, 517
BveI	ACCTGC	1 cut(s)	289
Cac8I	GCNNGC	2 cut(s)	282, 307
CaiI	CAGNNNCTG	2 cut(s)	88, 410
CfoI	GCGC	3 cut(s)	316, 389, 517
Cfr13I	GGNCC	3 cut(s)	134, 188, 380
ClaI	ATCGAT	2 cut(s)	146, 603
CseI	GACGC	1 cut(s)	251
Csp6I	GTAC	3 cut(s)	109, 415, 469
CviAII	CATG	1 cut(s)	281
CviQI	GTAC	3 cut(s)	109, 415, 469
DdeI	CTNAG	4 cut(s)	65, 140, 411, 615
DpnI	GATC	4 cut(s)	29, 145, 326, 602
DpnII	GATC	4 cut(s)	27, 143, 324, 600
DraIII	CACNNNGTG	1 cut(s)	487
EaeI	YGGCCR	2 cut(s)	3, 291
Eco130I	CCWWGG	2 cut(s)	55, 246
Eco47I	GGWCC	2 cut(s)	188, 380
Eco57I	CTGAAG	1 cut(s)	68
Eco91I	GGTNACC	1 cut(s)	172
EcoO65I	GGTNACC	1 cut(s)	172
EcoRII	CCWGG	2 cut(s)	87, 591
EcoT14I	CCWWGG	2 cut(s)	55, 246
ErhI	CCWWGG	2 cut(s)	55, 246
Esp3I	CGTCTC	1 cut(s)	629
FaeI	CATG	1 cut(s)	284
FaiI	YATR	3 cut(s)	282, 402, 570
FaqI	GGGAC	2 cut(s)	169, 174
FatI	CATG	1 cut(s)	280
Fnu4HI	GCNGC	7 cut(s)	75, 137, 336, 390, 405, 501, 513
FokI	GGATG	2 cut(s)	266, 464
Fsp4HI	GCNGC	7 cut(s)	75, 137, 336, 390, 405, 501, 513
FspBI	CTAG	1 cut(s)	56
FspI	TGCGCA	1 cut(s)	516
GlaI	GCGC	3 cut(s)	315, 388, 516
GluI	GCNGC	7 cut(s)	75, 137, 336, 390, 405, 501, 513
HaeIII	GGCC	3 cut(s)	5, 136, 293
HapII	CCGG	2 cut(s)	132, 199
HgaI	GACGC	1 cut(s)	251
HhaI	GCGC	3 cut(s)	316, 389, 517
Hin1I	GRCGYC	1 cut(s)	243
Hin1II	CATG	1 cut(s)	284
Hin6I	GCGC	3 cut(s)	314, 387, 515
HinP1I	GCGC	3 cut(s)	314, 387, 515
HinfI	GANTC	3 cut(s)	79, 103, 459
HpaII	CCGG	2 cut(s)	132, 199
HphI	GGTGA	1 cut(s)	166
Hpy166II	GTNNAC	2 cut(s)	196, 383
Hpy188I	TCNGA	5 cut(s)	66, 84, 143, 458, 600
Hpy188III	TCNNGA	4 cut(s)	154, 218, 328, 430
Hpy8I	GTNNAC	2 cut(s)	196, 383
Hpy99I	CGWCG	3 cut(s)	14, 185, 245
HpyAV	CCTTC	3 cut(s)	34, 483, 490
HpyCH4III	ACNGT	3 cut(s)	10, 160, 487
HpyCH4V	TGCA	4 cut(s)	96, 305, 404, 503
HpyF10VI	GCNNNNNNNGC	5 cut(s)	176, 290, 311, 497, 509
HpyF3I	CTNAG	4 cut(s)	65, 140, 411, 615
Hsp92I	GRCGYC	1 cut(s)	243
Hsp92II	CATG	1 cut(s)	284
HspAI	GCGC	3 cut(s)	314, 387, 515
Kzo9I	GATC	4 cut(s)	27, 143, 324, 600
LmnI	GCTCC	2 cut(s)	167, 289
Lsp1109I	GCAGC	5 cut(s)	347, 401, 416, 487, 499
MaeI	CTAG	1 cut(s)	56
MaeIII	GTNAC	1 cut(s)	172
MalI	GATC	4 cut(s)	29, 145, 326, 602
MbiI	CCGCTC	2 cut(s)	139, 170
MboI	GATC	4 cut(s)	27, 143, 324, 600
MboII	GAAGA	1 cut(s)	568
MlsI	TGGCCA	2 cut(s)	5, 293
MluCI	AATT	2 cut(s)	354, 658
MluNI	TGGCCA	2 cut(s)	5, 293
MlyI	GAGTC	1 cut(s)	453
MmeI	TCCRAC	1 cut(s)	436
MnlI	CCTC	6 cut(s)	60, 157, 239, 248, 358, 634
Mox20I	TGGCCA	2 cut(s)	5, 293
MscI	TGGCCA	2 cut(s)	5, 293
MseI	TTAA	2 cut(s)	207, 661
Msp20I	TGGCCA	2 cut(s)	5, 293
MspA1I	CMGCKG	2 cut(s)	338, 512
MspI	CCGG	2 cut(s)	132, 199
MspR9I	CCNGG	3 cut(s)	89, 133, 593
MvaI	CCWGG	2 cut(s)	89, 593
MvnI	CGCG	1 cut(s)	387
MwoI	GCNNNNNNNGC	5 cut(s)	176, 290, 311, 497, 509
NciI	CCSGG	1 cut(s)	133
NdeII	GATC	4 cut(s)	27, 143, 324, 600
NlaIII	CATG	1 cut(s)	284
NlaIV	GGNNCC	2 cut(s)	190, 597
NmuCI	GTSAC	1 cut(s)	172
NsbI	TGCGCA	1 cut(s)	516
NspI	RCATGY	1 cut(s)	284
NspV	TTCGAA	1 cut(s)	101
PaeI	GCATGC	1 cut(s)	284
PcsI	WCGNNNNNNNCGW	1 cut(s)	189
PfeI	GAWTC	2 cut(s)	79, 103
PflFI	GACNNNGTC	1 cut(s)	224
PflMI	CCANNNNNTGG	1 cut(s)	487
PkrI	GCNGC	7 cut(s)	76, 138, 337, 391, 406, 502, 514
Ple19I	CGATCG	1 cut(s)	603
PleI	GAGTC	1 cut(s)	453
PpsI	GAGTC	1 cut(s)	453
Psp6I	CCWGG	2 cut(s)	87, 591
PspEI	GGTNACC	1 cut(s)	172
PspGI	CCWGG	2 cut(s)	87, 591
PspN4I	GGNNCC	2 cut(s)	190, 597
PspPI	GGNCC	3 cut(s)	134, 188, 380
PstNI	CAGNNNCTG	2 cut(s)	88, 410
PsyI	GACNNNGTC	1 cut(s)	224
PvuI	CGATCG	1 cut(s)	603
RsaI	GTAC	3 cut(s)	110, 416, 470
RsaNI	GTAC	3 cut(s)	109, 415, 469
SaqAI	TTAA	2 cut(s)	207, 661
SatI	GCNGC	7 cut(s)	75, 137, 336, 390, 405, 501, 513
Sau3AI	GATC	4 cut(s)	27, 143, 324, 600
Sau96I	GGNCC	3 cut(s)	134, 188, 380
SchI	GAGTC	1 cut(s)	453
ScrFI	CCNGG	3 cut(s)	89, 133, 593
SetI	ASST	5 cut(s)	57, 252, 303, 394, 409
SfuI	TTCGAA	1 cut(s)	101
SinI	GGWCC	2 cut(s)	188, 380
SphI	GCATGC	1 cut(s)	284
Sse9I	AATT	2 cut(s)	354, 658
SsiI	CCGC	6 cut(s)	74, 137, 170, 177, 338, 510
SspMI	CTAG	1 cut(s)	56
StyD4I	CCNGG	3 cut(s)	87, 131, 591
StyI	CCWWGG	2 cut(s)	55, 246
TaaI	ACNGT	3 cut(s)	10, 160, 487
TaqI	TCGA	6 cut(s)	12, 101, 146, 240, 273, 603
TasI	AATT	2 cut(s)	354, 658
TatI	WGTACW	1 cut(s)	414
TauI	GCSGC	2 cut(s)	77, 139
TfiI	GAWTC	2 cut(s)	79, 103
Tru1I	TTAA	2 cut(s)	207, 661
Tru9I	TTAA	2 cut(s)	207, 661
TscAI	CASTG	3 cut(s)	323, 492, 524
TseFI	GTSAC	1 cut(s)	172
TseI	GCWGC	5 cut(s)	335, 389, 404, 500, 512
Tsp45I	GTSAC	1 cut(s)	172
TspDTI	ATGAA	1 cut(s)	270
TspGWI	ACGGA	1 cut(s)	653
TspRI	CASTG	3 cut(s)	323, 492, 524
Tth111I	GACNNNGTC	1 cut(s)	224
Van91I	CCANNNNNTGG	1 cut(s)	487
VpaK11BI	GGWCC	2 cut(s)	188, 380
XceI	RCATGY	1 cut(s)	284
XcmI	CCANNNNNNNNNTGG	1 cut(s)	484
XmaJI	CCTAGG	1 cut(s)	55
XspI	CTAG	1 cut(s)	56

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.