Rh4CG320900

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0011256
Physical Location & Seq
Reverse (-)
58330482 .. 58332816
2335 bp
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UTR
Exon/CDS
Intron
Rh4CG320900.1

Sequence Viewer

Length: 498 bp
ATGGGTATGGTATTGACAGTTGTACATTCTTTTCGACAGGTAGATGAAGCCACGGGCAAGTTTAACAAGCATGCTCCTCCTTTGGCTAAGCAGGTTGCAAGCCAAGGTCACAGTTTCCTCCAGACGACTTTGGAGAAGGGCCAAAAATTTGTGAAGGAGGCTCAAACTGGGGGTCCTCGCTCAGCCATTCATTATGCAGCTACAGAATCTAAGCAGCTTGTATTTAACCAATCTGTGAAGCTGTGGGTTGTACTTGACCAATACCATCCAATCCACAAAGTGGCAGAGAAGGCTGCACCAACAGCTGCTCACTTGTCCGAGAAATACAACCACACCATAAGGGACTGGACCGTGAAGGGATATACCATCTTCAGTTATTTGCCCTTGGTTCCGGTTGATGATATTTCCAAAGCAGTTGAGCAGGGTAAGGCTGGGAAGAAGGGAGATGCAGCTGTGCATGTTGAGCATAAATCTGATTCTGATTCTGATTCCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

165

Amino Acids

18.16

Weight (kDa)

8.69

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 12 - 141 5.8e-37 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67360 AT1G67360
fragaria_vesca FvH4_4g23790
malus_domestica MD01G1161800.v1.1 MD08G1165600.v1.1 MD13G1118800.v1.1 MD16G1119000.v1.1
prunus_persica Prupe.1G226000_v2.0.a1
pyrus_communis pycom13g10370 pycom16g10190
rosa_chinensis RchiOBHm_Chr4g0430281
rosa_laevigata RLG00000006988
rosa_multiflora Rmu_sc0001089.1_g000026
rosa_roxburghii Rroxscaffold_5G00372080
rosa_rugosa Rorug04G0242400
rosa_samantha Rh4AG298800 Rh4BG305400 Rh4CG320900 Rh4DG302700
rosa_wichuraiana Rw4G025910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 280
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 1 cut(s) 355
AdeI CACNNNGTG 1 cut(s) 280
AfaI GTAC 2 cut(s) 24, 252
AfiI CCNNNNNNNGG 1 cut(s) 280
AluBI AGCT 5 cut(s) 200, 217, 241, 305, 452
AluI AGCT 5 cut(s) 200, 217, 241, 305, 452
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 5 cut(s) 197, 214, 293, 305, 449
ApoI RAATTY 1 cut(s) 146
ArsI GACNNNNNNTTYG 2 cut(s) 157, 189
AspS9I GGNCC 3 cut(s) 139, 173, 348
AvaII GGWCC 2 cut(s) 173, 348
BbvI GCAGC 5 cut(s) 209, 226, 280, 292, 461
BccI CCATC 2 cut(s) 273, 374
BfmI CTRYAG 1 cut(s) 201
BfuAI ACCTGC 1 cut(s) 82
BisI GCNGC 5 cut(s) 198, 215, 294, 306, 450
BlpI GCTNAGC 2 cut(s) 87, 181
BlsI GCNGC 5 cut(s) 199, 216, 295, 307, 451
Bme18I GGWCC 2 cut(s) 173, 348
BmgT120I GGNCC 3 cut(s) 139, 173, 348
BmiI GGNNCC 2 cut(s) 174, 390
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 436
BmuI ACTGGG 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 104
Bpu1102I GCTNAGC 2 cut(s) 87, 181
BsaJI CCNNGG 3 cut(s) 51, 103, 384
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse1I ACTGG 2 cut(s) 172, 350
BseDI CCNNGG 3 cut(s) 51, 103, 384
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 172, 350
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 5 cut(s) 209, 226, 280, 292, 461
BseYI CCCAGC 1 cut(s) 431
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 1 cut(s) 141
Bsp1407I TGTACA 1 cut(s) 22
Bsp1720I GCTNAGC 2 cut(s) 87, 181
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 1 cut(s) 194
BspLI GGNNCC 2 cut(s) 174, 390
BspMI ACCTGC 1 cut(s) 82
BsrGI TGTACA 1 cut(s) 22
BsrI ACTGG 2 cut(s) 172, 350
BssECI CCNNGG 3 cut(s) 51, 103, 384
BssT1I CCWWGG 2 cut(s) 103, 384
Bst4CI ACNGT 3 cut(s) 19, 113, 352
BstAUI TGTACA 1 cut(s) 22
BstC8I GCNNGC 2 cut(s) 72, 100
BstDEI CTNAG 3 cut(s) 87, 181, 210
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 265
BstMWI GCNNNNNNNGC 3 cut(s) 290, 302, 463
BstNSI RCATGY 2 cut(s) 74, 461
BstSFI CTRYAG 1 cut(s) 201
BstV1I GCAGC 5 cut(s) 209, 226, 280, 292, 461
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 1 cut(s) 265
BveI ACCTGC 1 cut(s) 82
Cac8I GCNNGC 2 cut(s) 72, 100
Cfr13I GGNCC 3 cut(s) 139, 173, 348
Csp6I GTAC 2 cut(s) 23, 251
CviAII CATG 2 cut(s) 71, 458
CviQI GTAC 2 cut(s) 23, 251
DdeI CTNAG 3 cut(s) 87, 181, 210
DraIII CACNNNGTG 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 103, 384
Eco47I GGWCC 2 cut(s) 173, 348
Eco57I CTGAAG 1 cut(s) 355
EcoO109I RGGNCCY 1 cut(s) 173
EcoT14I CCWWGG 2 cut(s) 103, 384
ErhI CCWWGG 2 cut(s) 103, 384
FaeI CATG 2 cut(s) 74, 461
FaiI YATR 7 cut(s) 8, 72, 195, 338, 363, 459, 468
FaqI GGGAC 1 cut(s) 356
FatI CATG 2 cut(s) 70, 457
Fnu4HI GCNGC 5 cut(s) 198, 215, 294, 306, 450
FokI GGATG 1 cut(s) 252
Fsp4HI GCNGC 5 cut(s) 198, 215, 294, 306, 450
GluI GCNGC 5 cut(s) 198, 215, 294, 306, 450
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 104
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 392
Hin1II CATG 2 cut(s) 74, 461
HinfI GANTC 4 cut(s) 206, 476, 482, 488
HpaII CCGG 1 cut(s) 392
Hpy188I TCNGA 5 cut(s) 319, 475, 481, 487, 493
Hpy188III TCNNGA 1 cut(s) 121
HpyAV CCTTC 5 cut(s) 130, 148, 283, 349, 433
HpyCH4III ACNGT 3 cut(s) 19, 113, 352
HpyCH4V TGCA 5 cut(s) 98, 197, 296, 449, 457
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 302, 463
HpyF3I CTNAG 3 cut(s) 87, 181, 210
Hsp92II CATG 2 cut(s) 74, 461
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 8 cut(s) 23, 77, 134, 153, 331, 405, 407, 417
Lsp1109I GCAGC 5 cut(s) 209, 226, 280, 292, 461
LweI GCATC 1 cut(s) 436
MaeIII GTNAC 1 cut(s) 107
MboII GAAGA 2 cut(s) 361, 448
MluCI AATT 1 cut(s) 146
MnlI CCTC 4 cut(s) 87, 128, 151, 186
MseI TTAA 2 cut(s) 63, 225
MspA1I CMGCKG 2 cut(s) 305, 452
MspI CCGG 1 cut(s) 392
MwoI GCNNNNNNNGC 3 cut(s) 290, 302, 463
NlaIII CATG 2 cut(s) 74, 461
NlaIV GGNNCC 2 cut(s) 174, 390
NmuCI GTSAC 1 cut(s) 107
NspI RCATGY 2 cut(s) 74, 461
PaeI GCATGC 1 cut(s) 74
PfeI GAWTC 4 cut(s) 206, 476, 482, 488
PflMI CCANNNNNTGG 1 cut(s) 280
PkrI GCNGC 5 cut(s) 199, 216, 295, 307, 451
PpuMI RGGWCCY 1 cut(s) 173
Psp5II RGGWCCY 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 431
PspN4I GGNNCC 2 cut(s) 174, 390
PspPI GGNCC 3 cut(s) 139, 173, 348
PspPPI RGGWCCY 1 cut(s) 173
PvuII CAGCTG 2 cut(s) 305, 452
RsaI GTAC 2 cut(s) 24, 252
RsaNI GTAC 2 cut(s) 23, 251
SaqAI TTAA 2 cut(s) 63, 225
SatI GCNGC 5 cut(s) 198, 215, 294, 306, 450
Sau96I GGNCC 3 cut(s) 139, 173, 348
SetI ASST 8 cut(s) 42, 96, 109, 202, 219, 243, 307, 454
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 2 cut(s) 173, 348
SphI GCATGC 1 cut(s) 74
Sse9I AATT 1 cut(s) 146
StyI CCWWGG 2 cut(s) 103, 384
TaaI ACNGT 3 cut(s) 19, 113, 352
TaqI TCGA 1 cut(s) 34
TasI AATT 1 cut(s) 146
TatI WGTACW 2 cut(s) 22, 250
TfiI GAWTC 4 cut(s) 206, 476, 482, 488
Tru1I TTAA 2 cut(s) 63, 225
Tru9I TTAA 2 cut(s) 63, 225
TseFI GTSAC 1 cut(s) 107
TseI GCWGC 5 cut(s) 197, 214, 293, 305, 449
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 2 cut(s) 60, 179
Van91I CCANNNNNTGG 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 173, 348
XapI RAATTY 1 cut(s) 146
XceI RCATGY 2 cut(s) 74, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.