FvH4_4g23790

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011256
Physical Location & Seq
Reverse (-)
26161965 .. 26164434
2470 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23790.t1

Sequence Viewer

Length: 702 bp
ATGGCCACCGTTGAGAAGAAGGATTTGGAGTGCAAGGAGCTGAAGCATCTAGGGTTTGTGAGGATCGCAGCGATTCAGACGCTGGTGTGCGTTTCGAATCTGTACGAGTATGCGAAGCAGAACTCTGGGCCGTTGAGATCGACCGTTGGGACTGTGGAGGGTGCTGTCACCACCGTCGTGGGCCCGGTCTATAAGAAGTTCAAGGGCGTGCCTGATGATCTTCTTGCCTTTCTTGATACCAAGGTAGATGAAGCCACTGACAAGTTCAACAAGCATGCTCCTCCTTTGGCTAAGCAGGTTGCAAGCCAAGGTCACTATTTGGTCCAGACAGCTTGGGAGAAGGGCAAAAAACTCGTGAAGGAGGCCCAAACTGGGGGTCCTCGCTCAGCCATCCATTATGCAGCTACAGAATCTAAGCAGCTTGTATTTAACCAATCTGTGAAGTTGTGGGTTGTACTTGACCAGTACCATCCAATCCACAAAGTGGCAGAGAAGGCTGCACCAACAGCTGCTCACTTGTCCGAGAAGTACAACTATACCATAAAGGACTTGACCGTGAAGGGCTATCCCATCTTCGGTTATTTGCCCTTGGTTCCAGTTGATGATATATCCAAAGCAGTTAAGCAGGGAAAGGCTGGGAAGAAGGGGGATGCAGCTCCGCATCATGAGCATAAATCTGATTCTGATTCTGATTCCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

234

Amino Acids

25.52

Weight (kDa)

8.94

Isoelectric Point (pI)

33.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 11 - 209 3.5e-80 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67360 AT1G67360
fragaria_vesca FvH4_4g23790
malus_domestica MD01G1161800.v1.1 MD08G1165600.v1.1 MD13G1118800.v1.1 MD16G1119000.v1.1
prunus_persica Prupe.1G226000_v2.0.a1
pyrus_communis pycom13g10370 pycom16g10190
rosa_chinensis RchiOBHm_Chr4g0430281
rosa_laevigata RLG00000006988
rosa_multiflora Rmu_sc0001089.1_g000026
rosa_roxburghii Rroxscaffold_5G00372080
rosa_rugosa Rorug04G0242400
rosa_samantha Rh4AG298800 Rh4BG305400 Rh4CG320900 Rh4DG302700
rosa_wichuraiana Rw4G025910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 286
AccB7I CCANNNNNTGG 1 cut(s) 484
AciI CCGC 1 cut(s) 659
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 62
AdeI CACNNNGTG 1 cut(s) 484
AfaI GTAC 4 cut(s) 104, 456, 467, 530
AfiI CCNNNNNNNGG 4 cut(s) 372, 373, 484, 575
AgsI TTSAA 2 cut(s) 202, 268
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AleI CACNNNNGTG 1 cut(s) 176
AluBI AGCT 6 cut(s) 40, 332, 404, 421, 509, 656
AluI AGCT 6 cut(s) 40, 332, 404, 421, 509, 656
AlwI GGATC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 82
AoxI GGCC 4 cut(s) 3, 128, 181, 363
ApaI GGGCCC 1 cut(s) 185
ApeKI GCWGC 6 cut(s) 68, 401, 418, 497, 509, 653
ArsI GACNNNNNNTTYG 2 cut(s) 361, 393
AspS9I GGNCC 6 cut(s) 128, 181, 182, 322, 364, 377
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 160
AsuII TTCGAA 1 cut(s) 95
AvaII GGWCC 2 cut(s) 322, 377
BaeGI GKGCMC 1 cut(s) 185
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 185
BauI CACGAG 1 cut(s) 353
BbvI GCAGC 6 cut(s) 80, 413, 430, 484, 496, 665
BccI CCATC 3 cut(s) 398, 477, 578
BceAI ACGGC 1 cut(s) 115
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BcnI CCSGG 1 cut(s) 185
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 405
BfuAI ACCTGC 1 cut(s) 286
BisI GCNGC 6 cut(s) 69, 402, 419, 498, 510, 654
BlpI GCTNAGC 2 cut(s) 291, 385
BlsI GCNGC 6 cut(s) 70, 403, 420, 499, 511, 655
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 2 cut(s) 322, 377
BmgT120I GGNCC 6 cut(s) 128, 181, 182, 322, 364, 377
BmiI GGNNCC 3 cut(s) 183, 378, 594
BmrFI CCNGG 1 cut(s) 185
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 3 cut(s) 55, 640, 670
BmuI ACTGGG 1 cut(s) 381
Bpu1102I GCTNAGC 2 cut(s) 291, 385
Bpu14I TTCGAA 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 185
BsaJI CCNNGG 3 cut(s) 240, 307, 588
Bsc4I CCNNNNNNNGG 4 cut(s) 372, 373, 484, 575
Bse1I ACTGG 3 cut(s) 376, 463, 596
BseDI CCNNGG 3 cut(s) 240, 307, 588
BseGI GGATG 3 cut(s) 390, 469, 655
BseLI CCNNNNNNNGG 4 cut(s) 372, 373, 484, 575
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 3 cut(s) 376, 463, 596
BseRI GAGGAG 1 cut(s) 270
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 6 cut(s) 80, 413, 430, 484, 496, 665
BseYI CCCAGC 1 cut(s) 635
BsgI GTGCAG 1 cut(s) 483
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 4 cut(s) 5, 130, 183, 365
BsiEI CGRYCG 1 cut(s) 144
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 4 cut(s) 372, 373, 484, 575
BsmFI GGGAC 1 cut(s) 163
BsnI GGCC 4 cut(s) 5, 130, 183, 365
Bsp119I TTCGAA 1 cut(s) 95
Bsp120I GGGCCC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 3 cut(s) 63, 137, 217
Bsp1720I GCTNAGC 2 cut(s) 291, 385
BspACI CCGC 1 cut(s) 659
BspANI GGCC 4 cut(s) 5, 130, 183, 365
BspCNI CTCAG 1 cut(s) 398
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 3 cut(s) 183, 378, 594
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 95
BsrI ACTGG 3 cut(s) 376, 463, 596
BssECI CCNNGG 3 cut(s) 240, 307, 588
BssMI GATC 3 cut(s) 63, 137, 217
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 3 cut(s) 240, 307, 588
Bst2BI CACGAG 1 cut(s) 353
Bst4CI ACNGT 5 cut(s) 10, 145, 154, 175, 556
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 3 cut(s) 209, 276, 304
BstDEI CTNAG 3 cut(s) 291, 385, 414
BstF5I GGATG 3 cut(s) 390, 469, 655
BstKTI GATC 3 cut(s) 66, 140, 220
BstMBI GATC 3 cut(s) 63, 137, 217
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 3 cut(s) 494, 506, 667
BstNSI RCATGY 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 1 cut(s) 185
BstV1I GCAGC 6 cut(s) 80, 413, 430, 484, 496, 665
BstXI CCANNNNNNTGG 1 cut(s) 178
BsuRI GGCC 4 cut(s) 5, 130, 183, 365
BtsCI GGATG 3 cut(s) 390, 469, 655
BtsIMutI CAGTG 1 cut(s) 255
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 209, 276, 304
CaiI CAGNNNCTG 1 cut(s) 82
CciI TCATGA 1 cut(s) 664
Cfr13I GGNCC 6 cut(s) 128, 181, 182, 322, 364, 377
CseI GACGC 1 cut(s) 88
Csp6I GTAC 4 cut(s) 103, 455, 466, 529
CviAII CATG 2 cut(s) 275, 665
CviQI GTAC 4 cut(s) 103, 455, 466, 529
DdeI CTNAG 3 cut(s) 291, 385, 414
DpnI GATC 3 cut(s) 65, 139, 219
DpnII GATC 3 cut(s) 63, 137, 217
DraIII CACNNNGTG 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 3 cut(s) 240, 307, 588
Eco24I GRGCYC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 322, 377
Eco57I CTGAAG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 377
EcoT14I CCWWGG 3 cut(s) 240, 307, 588
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 3 cut(s) 240, 307, 588
FaeI CATG 2 cut(s) 278, 668
FaiI YATR 9 cut(s) 111, 192, 276, 399, 537, 542, 608, 666, 672
FaqI GGGAC 1 cut(s) 163
FatI CATG 2 cut(s) 274, 664
Fnu4HI GCNGC 6 cut(s) 69, 402, 419, 498, 510, 654
FokI GGATG 3 cut(s) 377, 456, 662
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 6 cut(s) 69, 402, 419, 498, 510, 654
FspBI CTAG 1 cut(s) 50
GluI GCNGC 6 cut(s) 69, 402, 419, 498, 510, 654
GsaI CCCAGC 1 cut(s) 639
HaeIII GGCC 4 cut(s) 5, 130, 183, 365
HapII CCGG 1 cut(s) 185
HgaI GACGC 1 cut(s) 88
Hin1II CATG 2 cut(s) 278, 668
HinfI GANTC 6 cut(s) 73, 97, 410, 680, 686, 692
HpaII CCGG 1 cut(s) 185
HphI GGTGA 1 cut(s) 160
Hpy188I TCNGA 6 cut(s) 78, 523, 679, 685, 691, 697
Hpy188III TCNNGA 4 cut(s) 233, 325, 355, 665
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 6 cut(s) 13, 334, 352, 487, 553, 637
HpyCH4III ACNGT 5 cut(s) 10, 145, 154, 175, 556
HpyCH4V TGCA 5 cut(s) 33, 302, 401, 500, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 506, 667
HpyF3I CTNAG 3 cut(s) 291, 385, 414
Hsp92II CATG 2 cut(s) 278, 668
Kzo9I GATC 3 cut(s) 63, 137, 217
LmnI GCTCC 3 cut(s) 37, 283, 661
Lsp1109I GCAGC 6 cut(s) 80, 413, 430, 484, 496, 665
LweI GCATC 3 cut(s) 55, 640, 670
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 2 cut(s) 166, 311
MalI GATC 3 cut(s) 65, 139, 219
MboI GATC 3 cut(s) 63, 137, 217
MboII GAAGA 4 cut(s) 28, 212, 565, 652
MhlI GDGCHC 1 cut(s) 185
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 5 cut(s) 54, 151, 291, 355, 390
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 429, 621
MslI CAYNNNNRTG 1 cut(s) 176
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 1 cut(s) 185
MwoI GCNNNNNNNGC 3 cut(s) 494, 506, 667
NciI CCSGG 1 cut(s) 185
NdeII GATC 3 cut(s) 63, 137, 217
NlaIII CATG 2 cut(s) 278, 668
NlaIV GGNNCC 3 cut(s) 183, 378, 594
NmuCI GTSAC 2 cut(s) 166, 311
NspI RCATGY 1 cut(s) 278
NspV TTCGAA 1 cut(s) 95
OliI CACNNNNGTG 1 cut(s) 176
PaeI GCATGC 1 cut(s) 278
PagI TCATGA 1 cut(s) 664
PfeI GAWTC 6 cut(s) 73, 97, 410, 680, 686, 692
PflMI CCANNNNNTGG 1 cut(s) 484
PkrI GCNGC 6 cut(s) 70, 403, 420, 499, 511, 655
PpuMI RGGWCCY 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 377
PspFI CCCAGC 1 cut(s) 635
PspN4I GGNNCC 3 cut(s) 183, 378, 594
PspOMI GGGCCC 1 cut(s) 181
PspPI GGNCC 6 cut(s) 128, 181, 182, 322, 364, 377
PspPPI RGGWCCY 1 cut(s) 377
PstNI CAGNNNCTG 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 509
RsaI GTAC 4 cut(s) 104, 456, 467, 530
RsaNI GTAC 4 cut(s) 103, 455, 466, 529
RseI CAYNNNNRTG 1 cut(s) 176
SaqAI TTAA 2 cut(s) 429, 621
SatI GCNGC 6 cut(s) 69, 402, 419, 498, 510, 654
Sau3AI GATC 3 cut(s) 63, 137, 217
Sau96I GGNCC 6 cut(s) 128, 181, 182, 322, 364, 377
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 185
SetI ASST 9 cut(s) 42, 246, 300, 313, 334, 406, 423, 511, 658
SfaNI GCATC 3 cut(s) 55, 640, 670
SfcI CTRYAG 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 95
SinI GGWCC 2 cut(s) 322, 377
SmiMI CAYNNNNRTG 1 cut(s) 176
SphI GCATGC 1 cut(s) 278
SsiI CCGC 1 cut(s) 659
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 3 cut(s) 240, 307, 588
TaaI ACNGT 5 cut(s) 10, 145, 154, 175, 556
TaqI TCGA 2 cut(s) 95, 140
TatI WGTACW 2 cut(s) 454, 528
TfiI GAWTC 6 cut(s) 73, 97, 410, 680, 686, 692
Tru1I TTAA 2 cut(s) 429, 621
Tru9I TTAA 2 cut(s) 429, 621
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 166, 311
TseI GCWGC 6 cut(s) 68, 401, 418, 497, 509, 653
Tsp45I GTSAC 2 cut(s) 166, 311
TspDTI ATGAA 1 cut(s) 264
TspRI CASTG 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 322, 377
XceI RCATGY 1 cut(s) 278
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.