MD13G1118800.v1.1

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011256
Physical Location & Seq
Forward (+)
8684774 .. 8686689
1916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1118800.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGCCACCGTCGATAAGGGAGTAGAGATCGGCAAGGAGAAGGAACTGAAGCACCTAGGGTTTCTGAGGGTGGCGGCGATTCAGACCCTGGTGTGCGTTTCGAATCTGTACGATTACGCCAAGCAGAACTCCGGGCCGCTCAGATCGACGGTCGGTACTGTGGAGGGCGCGGTCACCGCCGTCGTTGGTCCCGTTTACCGGAAGTTTAAGGGCGTTCCTGACGACGTCCTCGCTTTCCTCGACGCCAAGGTGGATGAAGCCACGGACAAGTTCGACAAGCATGCTCCTCCTGTGGCTAAGCAGGTTGCGAGCCAAGCGCACTGCTTGATCCAGAAAGCAGCGGAAAAGGGGCAGAGAATTGTAAGGGAGGCTGAAACTGGCGGTCCACGCGCAGCTATTCACTATGCAGCTACTGAGTACAAGAACTTTGTCTTGAACCAATCTGTGAAGTTGTGGGTCGGACTCAACAAGTACCCTTCCATCCACACAGTGGCACAGAAGGCTGCACCAACCGCTGCGCACTGGTCTGGCAAATACAACCACACCGTAAAGGACTTGACCCGTAAGGGCTATGCCATCTTCGGCTATCTACCCTTGGTTCCGATCGATGAGATGTCCAAGGCAGTCGAAAAGGGAGAGGCGGAGAAGAAAGAAGATGCGGCTGCACATGTCGAGCACAAATCGGATTCTTCTGATTCTGATTGA

Protein Analysis

235

Amino Acids

25.43

Weight (kDa)

8.64

Isoelectric Point (pI)

28.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 12 - 210 4.8e-85 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67360 AT1G67360
fragaria_vesca FvH4_4g23790
malus_domestica MD01G1161800.v1.1 MD08G1165600.v1.1 MD13G1118800.v1.1 MD16G1119000.v1.1
prunus_persica Prupe.1G226000_v2.0.a1
pyrus_communis pycom13g10370 pycom16g10190
rosa_chinensis RchiOBHm_Chr4g0430281
rosa_laevigata RLG00000006988
rosa_multiflora Rmu_sc0001089.1_g000026
rosa_roxburghii Rroxscaffold_5G00372080
rosa_rugosa Rorug04G0242400
rosa_samantha Rh4AG298800 Rh4BG305400 Rh4CG320900 Rh4DG302700
rosa_wichuraiana Rw4G025910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 228
Acc16I TGCGCA 1 cut(s) 519
Acc36I ACCTGC 1 cut(s) 292
AccB7I CCANNNNNTGG 1 cut(s) 490
AccBSI CCGCTC 1 cut(s) 139
AccII CGCG 2 cut(s) 170, 390
AciI CCGC 9 cut(s) 74, 137, 170, 177, 341, 381, 513, 641, 659
AclWI GGATC 1 cut(s) 322
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 68
AcyI GRCGYC 2 cut(s) 225, 243
AdeI CACNNNGTG 1 cut(s) 490
AfaI GTAC 4 cut(s) 110, 157, 419, 473
AfiI CCNNNNNNNGG 2 cut(s) 198, 490
AflIII ACRYGT 1 cut(s) 667
AgsI TTSAA 1 cut(s) 436
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 2 cut(s) 395, 410
AluI AGCT 2 cut(s) 395, 410
Alw21I GWGCWC 1 cut(s) 678
AlwI GGATC 1 cut(s) 322
AlwNI CAGNNNCTG 2 cut(s) 88, 413
AoxI GGCC 2 cut(s) 3, 134
ApeKI GCWGC 6 cut(s) 338, 392, 407, 503, 515, 662
AspA2I CCTAGG 1 cut(s) 55
AspLEI GCGC 4 cut(s) 170, 319, 392, 520
AspS9I GGNCC 3 cut(s) 134, 188, 383
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 166
AsuII TTCGAA 1 cut(s) 101
AvaII GGWCC 2 cut(s) 188, 383
AvrII CCTAGG 1 cut(s) 55
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 678
BbvI GCAGC 6 cut(s) 350, 404, 419, 490, 502, 649
BccI CCATC 2 cut(s) 488, 584
BceAI ACGGC 1 cut(s) 164
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 89
BcnI CCSGG 1 cut(s) 133
BfaI CTAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 292
BisI GCNGC 9 cut(s) 75, 137, 339, 393, 408, 504, 516, 660, 663
BlnI CCTAGG 1 cut(s) 55
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 9 cut(s) 76, 138, 340, 394, 409, 505, 517, 661, 664
Bme1390I CCNGG 2 cut(s) 89, 133
Bme18I GGWCC 2 cut(s) 188, 383
BmgT120I GGNCC 3 cut(s) 134, 188, 383
BmiI GGNNCC 2 cut(s) 190, 600
BmrFI CCNGG 2 cut(s) 89, 133
BmsI GCATC 1 cut(s) 646
Bpu1102I GCTNAGC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 606
BsaHI GRCGYC 2 cut(s) 225, 243
BsaJI CCNNGG 6 cut(s) 55, 87, 246, 261, 594, 618
BsaWI WCCGGW 1 cut(s) 198
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 490
Bse1I ACTGG 2 cut(s) 382, 527
BseBI CCWGG 1 cut(s) 89
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 6 cut(s) 55, 87, 246, 261, 594, 618
BseGI GGATG 2 cut(s) 259, 480
BseLI CCNNNNNNNGG 2 cut(s) 198, 490
BseMII CTCAG 3 cut(s) 56, 154, 405
BseNI ACTGG 2 cut(s) 382, 527
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 6 cut(s) 350, 404, 419, 490, 502, 649
BsgI GTGCAG 2 cut(s) 489, 648
Bsh1236I CGCG 2 cut(s) 170, 390
Bsh1285I CGRYCG 2 cut(s) 153, 606
BshFI GGCC 2 cut(s) 5, 136
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 2 cut(s) 153, 606
BsiHKAI GWGCWC 1 cut(s) 678
BsiSI CCGG 2 cut(s) 132, 199
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 2 cut(s) 198, 490
BsmFI GGGAC 1 cut(s) 174
BsnI GGCC 2 cut(s) 5, 136
Bsp119I TTCGAA 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 678
Bsp143I GATC 4 cut(s) 27, 143, 327, 603
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 9 cut(s) 74, 137, 170, 177, 341, 381, 513, 641, 659
BspANI GGCC 2 cut(s) 5, 136
BspCNI CTCAG 3 cut(s) 57, 153, 406
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 2 cut(s) 170, 390
BspLI GGNNCC 2 cut(s) 190, 600
BspMI ACCTGC 1 cut(s) 292
BspPI GGATC 1 cut(s) 322
BspT104I TTCGAA 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 139
BsrI ACTGG 2 cut(s) 382, 527
BssECI CCNNGG 6 cut(s) 55, 87, 246, 261, 594, 618
BssMI GATC 4 cut(s) 27, 143, 327, 603
BssNI GRCGYC 2 cut(s) 225, 243
BssT1I CCWWGG 4 cut(s) 55, 246, 594, 618
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 5 cut(s) 10, 151, 160, 490, 547
BstACI GRCGYC 2 cut(s) 225, 243
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 2 cut(s) 282, 310
BstDEI CTNAG 4 cut(s) 65, 140, 297, 414
BstDSI CCRYGG 1 cut(s) 261
BstEII GGTNACC 1 cut(s) 172
BstF5I GGATG 2 cut(s) 259, 480
BstFNI CGCG 2 cut(s) 170, 390
BstHHI GCGC 4 cut(s) 170, 319, 392, 520
BstKTI GATC 4 cut(s) 30, 146, 330, 606
BstMBI GATC 4 cut(s) 27, 143, 327, 603
BstMCI CGRYCG 2 cut(s) 153, 606
BstMWI GCNNNNNNNGC 5 cut(s) 176, 314, 387, 500, 512
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 2 cut(s) 284, 671
BstPI GGTNACC 1 cut(s) 172
BstSCI CCNGG 2 cut(s) 87, 131
BstUI CGCG 2 cut(s) 170, 390
BstV1I GCAGC 6 cut(s) 350, 404, 419, 490, 502, 649
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 2 cut(s) 5, 136
BsuTUI ATCGAT 1 cut(s) 606
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 259, 480
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 3 cut(s) 319, 495, 520
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 2 cut(s) 282, 310
CaiI CAGNNNCTG 2 cut(s) 88, 413
CfoI GCGC 4 cut(s) 170, 319, 392, 520
Cfr13I GGNCC 3 cut(s) 134, 188, 383
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 1 cut(s) 251
Csp6I GTAC 4 cut(s) 109, 156, 418, 472
CviAII CATG 2 cut(s) 281, 668
CviQI GTAC 4 cut(s) 109, 156, 418, 472
DdeI CTNAG 4 cut(s) 65, 140, 297, 414
DpnI GATC 4 cut(s) 29, 145, 329, 605
DpnII GATC 4 cut(s) 27, 143, 327, 603
DraIII CACNNNGTG 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 656
Eco130I CCWWGG 4 cut(s) 55, 246, 594, 618
Eco47I GGWCC 2 cut(s) 188, 383
Eco57I CTGAAG 1 cut(s) 68
Eco91I GGTNACC 1 cut(s) 172
EcoO65I GGTNACC 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 4 cut(s) 55, 246, 594, 618
ErhI CCWWGG 4 cut(s) 55, 246, 594, 618
FaeI CATG 2 cut(s) 284, 671
FaiI YATR 4 cut(s) 282, 405, 573, 669
FaqI GGGAC 1 cut(s) 174
FatI CATG 2 cut(s) 280, 667
Fnu4HI GCNGC 9 cut(s) 75, 137, 339, 393, 408, 504, 516, 660, 663
FokI GGATG 2 cut(s) 266, 467
Fsp4HI GCNGC 9 cut(s) 75, 137, 339, 393, 408, 504, 516, 660, 663
FspBI CTAG 1 cut(s) 56
FspI TGCGCA 1 cut(s) 519
GlaI GCGC 4 cut(s) 169, 318, 391, 519
GluI GCNGC 9 cut(s) 75, 137, 339, 393, 408, 504, 516, 660, 663
HaeIII GGCC 2 cut(s) 5, 136
HapII CCGG 2 cut(s) 132, 199
HgaI GACGC 1 cut(s) 251
HhaI GCGC 4 cut(s) 170, 319, 392, 520
Hin1I GRCGYC 2 cut(s) 225, 243
Hin1II CATG 2 cut(s) 284, 671
Hin6I GCGC 4 cut(s) 168, 317, 390, 518
HinP1I GCGC 4 cut(s) 168, 317, 390, 518
HinfI GANTC 5 cut(s) 79, 103, 462, 686, 695
HpaII CCGG 2 cut(s) 132, 199
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 196, 386
Hpy188I TCNGA 8 cut(s) 66, 84, 143, 461, 603, 685, 694, 700
Hpy188III TCNNGA 3 cut(s) 218, 331, 433
Hpy8I GTNNAC 2 cut(s) 196, 386
Hpy99I CGWCG 5 cut(s) 14, 151, 185, 227, 245
HpyAV CCTTC 3 cut(s) 34, 486, 493
HpyCH4III ACNGT 5 cut(s) 10, 151, 160, 490, 547
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 407, 506, 665
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 314, 387, 500, 512
HpyF3I CTNAG 4 cut(s) 65, 140, 297, 414
HpySE526I ACGT 1 cut(s) 225
Hsp92I GRCGYC 2 cut(s) 225, 243
Hsp92II CATG 2 cut(s) 284, 671
HspAI GCGC 4 cut(s) 168, 317, 390, 518
Kzo9I GATC 4 cut(s) 27, 143, 327, 603
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 6 cut(s) 350, 404, 419, 490, 502, 649
LweI GCATC 1 cut(s) 646
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 172
MalI GATC 4 cut(s) 29, 145, 329, 605
MbiI CCGCTC 1 cut(s) 139
MboI GATC 4 cut(s) 27, 143, 327, 603
MboII GAAGA 4 cut(s) 571, 658, 665, 681
MhlI GDGCHC 1 cut(s) 678
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 357
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 7 cut(s) 60, 157, 239, 248, 297, 361, 631
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 341, 515
MspI CCGG 2 cut(s) 132, 199
MspR9I CCNGG 2 cut(s) 89, 133
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 2 cut(s) 170, 390
MwoI GCNNNNNNNGC 5 cut(s) 176, 314, 387, 500, 512
NciI CCSGG 1 cut(s) 133
NdeII GATC 4 cut(s) 27, 143, 327, 603
NlaIII CATG 2 cut(s) 284, 671
NlaIV GGNNCC 2 cut(s) 190, 600
NmuCI GTSAC 1 cut(s) 172
NsbI TGCGCA 1 cut(s) 519
NspI RCATGY 2 cut(s) 284, 671
NspV TTCGAA 1 cut(s) 101
PaeI GCATGC 1 cut(s) 284
PciI ACATGT 1 cut(s) 667
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 237
PfeI GAWTC 4 cut(s) 79, 103, 686, 695
PflFI GACNNNGTC 1 cut(s) 224
PflMI CCANNNNNTGG 1 cut(s) 490
PkrI GCNGC 9 cut(s) 76, 138, 340, 394, 409, 505, 517, 661, 664
Ple19I CGATCG 1 cut(s) 606
PleI GAGTC 1 cut(s) 456
PpsI GAGTC 1 cut(s) 456
PscI ACATGT 1 cut(s) 667
Psp6I CCWGG 1 cut(s) 87
PspEI GGTNACC 1 cut(s) 172
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 190, 600
PspPI GGNCC 3 cut(s) 134, 188, 383
PstNI CAGNNNCTG 2 cut(s) 88, 413
PsyI GACNNNGTC 1 cut(s) 224
PvuI CGATCG 1 cut(s) 606
RsaI GTAC 4 cut(s) 110, 157, 419, 473
RsaNI GTAC 4 cut(s) 109, 156, 418, 472
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 9 cut(s) 75, 137, 339, 393, 408, 504, 516, 660, 663
Sau3AI GATC 4 cut(s) 27, 143, 327, 603
Sau96I GGNCC 3 cut(s) 134, 188, 383
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 2 cut(s) 89, 133
SduI GDGCHC 1 cut(s) 678
SetI ASST 6 cut(s) 57, 228, 252, 306, 397, 412
SfaNI GCATC 1 cut(s) 646
SfuI TTCGAA 1 cut(s) 101
SinI GGWCC 2 cut(s) 188, 383
SphI GCATGC 1 cut(s) 284
Sse9I AATT 1 cut(s) 357
SsiI CCGC 9 cut(s) 74, 137, 170, 177, 341, 381, 513, 641, 659
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 2 cut(s) 87, 131
StyI CCWWGG 4 cut(s) 55, 246, 594, 618
TaaI ACNGT 5 cut(s) 10, 151, 160, 490, 547
TaiI ACGT 1 cut(s) 228
TaqI TCGA 8 cut(s) 12, 101, 146, 240, 273, 606, 627, 672
TasI AATT 1 cut(s) 357
TatI WGTACW 1 cut(s) 417
TauI GCSGC 3 cut(s) 77, 139, 662
TfiI GAWTC 4 cut(s) 79, 103, 686, 695
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 3 cut(s) 326, 495, 527
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 6 cut(s) 338, 392, 407, 503, 515, 662
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 270
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 3 cut(s) 326, 495, 527
Tth111I GACNNNGTC 1 cut(s) 224
Van91I CCANNNNNTGG 1 cut(s) 490
VpaK11BI GGWCC 2 cut(s) 188, 383
XceI RCATGY 2 cut(s) 284, 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
XmaJI CCTAGG 1 cut(s) 55
XspI CTAG 1 cut(s) 56
ZraI GACGTC 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.