RLG00000006988

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011256
Physical Location & Seq
Forward (+)
12472069 .. 12474019
1951 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006988

Sequence Viewer

Length: 708 bp
ATGGCCACCGTCGAGAAGGATTTAGAGTGCAAGGTTGGGAACGAGAAGGCGCTGAAGCACCTAGGGTTTGTGAGGATCGCGGCGATTCAGACTCTGTTGTGCGTTTCGAATCTGTACGAGTATGCGAAGCAGAACTCTGGACCTCTGAGATCGACAGTTGGGACTGTGGAGGGTGCTGTCACCACCGTCGTTGGTCCGGTCTATGAGAAGCTCAAGGGCGTACCTGATGATCTTCTTGCTTTTCTTGACACCAAGGTAGATGAAGCCACGGGCAAGTTTAACAAGCATGCTCCTCCTTTGGCTAAGCAGGTTGCAAGCCAAGGTCACAGATTGATCCAGACAACTTTGGAGAAGGGCCAAAAATTTGTGAAGGAGGCTCAAACTGGGGGTCCTCGCTCAGCCATTCATTATGCAGCTACAGAATCTAAGCAGCTTGTATTTAACCAATCTGTGAAGCTGTGGGTTGTACTTGACCAGTACCATCCAATCCACAAAGTGGCAGAGAAGGCTGCACCAACAGCTGCTCACTTGTCCGAGAAATACAACCACACCATAAGGGACTTGACCGTGAAGGGATATACCATCTTCGGTTATTTGCCCTTGGTTCCGGTTGATGATATTTCCAAAGCAGTTAATCAGGGTAAGGCTGAGAAGAAGGGAGATGCAGCTGTGCATGTTGAGCATAAATCTGATTCTGATTCCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

236

Amino Acids

25.61

Weight (kDa)

8.65

Isoelectric Point (pI)

27.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 14 - 212 5.3e-80 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67360 AT1G67360
fragaria_vesca FvH4_4g23790
malus_domestica MD01G1161800.v1.1 MD08G1165600.v1.1 MD13G1118800.v1.1 MD16G1119000.v1.1
prunus_persica Prupe.1G226000_v2.0.a1
pyrus_communis pycom13g10370 pycom16g10190
rosa_chinensis RchiOBHm_Chr4g0430281
rosa_laevigata RLG00000006988
rosa_multiflora Rmu_sc0001089.1_g000026
rosa_roxburghii Rroxscaffold_5G00372080
rosa_rugosa Rorug04G0242400
rosa_samantha Rh4AG298800 Rh4BG305400 Rh4CG320900 Rh4DG302700
rosa_wichuraiana Rw4G025910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 298
AccB7I CCANNNNNTGG 1 cut(s) 496
AccII CGCG 1 cut(s) 80
AciI CCGC 1 cut(s) 80
AclWI GGATC 2 cut(s) 83, 328
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 74
AdeI CACNNNGTG 1 cut(s) 496
AfaI GTAC 4 cut(s) 116, 222, 468, 479
AfiI CCNNNNNNNGG 1 cut(s) 496
AluBI AGCT 6 cut(s) 211, 416, 433, 457, 521, 668
AluI AGCT 6 cut(s) 211, 416, 433, 457, 521, 668
AlwI GGATC 2 cut(s) 83, 328
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 2 cut(s) 3, 355
ApeKI GCWGC 5 cut(s) 413, 430, 509, 521, 665
ApoI RAATTY 1 cut(s) 362
ArsI GACNNNNNNTTYG 2 cut(s) 373, 405
AspA2I CCTAGG 1 cut(s) 61
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 4 cut(s) 140, 194, 355, 389
AsuHPI GGTGA 1 cut(s) 172
AsuII TTCGAA 1 cut(s) 107
AvaII GGWCC 3 cut(s) 140, 194, 389
AvrII CCTAGG 1 cut(s) 61
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 5 cut(s) 425, 442, 496, 508, 677
BccI CCATC 2 cut(s) 489, 590
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 417
BfoI RGCGCY 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 6 cut(s) 81, 414, 431, 510, 522, 666
BlnI CCTAGG 1 cut(s) 61
BlpI GCTNAGC 2 cut(s) 303, 397
BlsI GCNGC 6 cut(s) 82, 415, 432, 511, 523, 667
Bme18I GGWCC 3 cut(s) 140, 194, 389
BmgT120I GGNCC 4 cut(s) 140, 194, 355, 389
BmiI GGNNCC 2 cut(s) 390, 606
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 1 cut(s) 652
BmuI ACTGGG 1 cut(s) 393
Bpu1102I GCTNAGC 2 cut(s) 303, 397
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 197
BsaJI CCNNGG 5 cut(s) 61, 252, 267, 319, 600
BsaWI WCCGGW 2 cut(s) 196, 607
Bsc4I CCNNNNNNNGG 1 cut(s) 496
Bse1I ACTGG 2 cut(s) 388, 475
BseDI CCNNGG 5 cut(s) 61, 252, 267, 319, 600
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 1 cut(s) 496
BseMII CTCAG 3 cut(s) 137, 411, 639
BseNI ACTGG 2 cut(s) 388, 475
BseRI GAGGAG 1 cut(s) 282
BseXI GCAGC 5 cut(s) 425, 442, 496, 508, 677
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 2 cut(s) 5, 357
BsiSI CCGG 2 cut(s) 197, 608
BslFI GGGAC 2 cut(s) 175, 572
BslI CCNNNNNNNGG 1 cut(s) 496
BsmFI GGGAC 2 cut(s) 175, 572
BsnI GGCC 2 cut(s) 5, 357
Bsp119I TTCGAA 1 cut(s) 107
Bsp143I GATC 4 cut(s) 75, 149, 229, 333
Bsp1720I GCTNAGC 2 cut(s) 303, 397
BspACI CCGC 1 cut(s) 80
BspANI GGCC 2 cut(s) 5, 357
BspCNI CTCAG 3 cut(s) 138, 410, 640
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 2 cut(s) 390, 606
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 2 cut(s) 83, 328
BspT104I TTCGAA 1 cut(s) 107
BsrI ACTGG 2 cut(s) 388, 475
BssECI CCNNGG 5 cut(s) 61, 252, 267, 319, 600
BssMI GATC 4 cut(s) 75, 149, 229, 333
BssT1I CCWWGG 4 cut(s) 61, 252, 319, 600
Bst4CI ACNGT 5 cut(s) 10, 157, 166, 187, 568
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 2 cut(s) 288, 316
BstDEI CTNAG 5 cut(s) 146, 303, 397, 426, 648
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 481
BstFNI CGCG 1 cut(s) 80
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 4 cut(s) 78, 152, 232, 336
BstMBI GATC 4 cut(s) 75, 149, 229, 333
BstMWI GCNNNNNNNGC 3 cut(s) 506, 518, 679
BstNSI RCATGY 2 cut(s) 290, 677
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 5 cut(s) 425, 442, 496, 508, 677
BsuRI GGCC 2 cut(s) 5, 357
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 1 cut(s) 481
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 2 cut(s) 288, 316
CaiI CAGNNNCTG 1 cut(s) 94
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 4 cut(s) 140, 194, 355, 389
Csp6I GTAC 4 cut(s) 115, 221, 467, 478
CspCI CAANNNNNGTGG 2 cut(s) 172, 207
CviAII CATG 2 cut(s) 287, 674
CviQI GTAC 4 cut(s) 115, 221, 467, 478
DdeI CTNAG 5 cut(s) 146, 303, 397, 426, 648
DpnI GATC 4 cut(s) 77, 151, 231, 335
DpnII GATC 4 cut(s) 75, 149, 229, 333
DraIII CACNNNGTG 1 cut(s) 496
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 4 cut(s) 61, 252, 319, 600
Eco47I GGWCC 3 cut(s) 140, 194, 389
Eco57I CTGAAG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 389
EcoT14I CCWWGG 4 cut(s) 61, 252, 319, 600
ErhI CCWWGG 4 cut(s) 61, 252, 319, 600
FaeI CATG 2 cut(s) 290, 677
FaiI YATR 8 cut(s) 123, 204, 288, 411, 554, 579, 675, 684
FaqI GGGAC 2 cut(s) 175, 572
FatI CATG 2 cut(s) 286, 673
Fnu4HI GCNGC 6 cut(s) 81, 414, 431, 510, 522, 666
FokI GGATG 1 cut(s) 468
Fsp4HI GCNGC 6 cut(s) 81, 414, 431, 510, 522, 666
FspBI CTAG 1 cut(s) 62
GlaI GCGC 1 cut(s) 51
GluI GCNGC 6 cut(s) 81, 414, 431, 510, 522, 666
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 2 cut(s) 5, 357
HapII CCGG 2 cut(s) 197, 608
HhaI GCGC 1 cut(s) 52
Hin1II CATG 2 cut(s) 290, 677
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HinfI GANTC 6 cut(s) 85, 91, 109, 422, 692, 698
HpaII CCGG 2 cut(s) 197, 608
HphI GGTGA 1 cut(s) 172
Hpy188I TCNGA 6 cut(s) 90, 147, 535, 691, 697, 703
Hpy188III TCNNGA 4 cut(s) 13, 138, 245, 337
Hpy99I CGWCG 2 cut(s) 14, 191
HpyAV CCTTC 7 cut(s) 10, 40, 346, 364, 499, 565, 649
HpyCH4III ACNGT 5 cut(s) 10, 157, 166, 187, 568
HpyCH4V TGCA 6 cut(s) 30, 314, 413, 512, 665, 673
HpyF10VI GCNNNNNNNGC 3 cut(s) 506, 518, 679
HpyF3I CTNAG 5 cut(s) 146, 303, 397, 426, 648
Hsp92II CATG 2 cut(s) 290, 677
HspAI GCGC 1 cut(s) 50
Kzo9I GATC 4 cut(s) 75, 149, 229, 333
LmnI GCTCC 1 cut(s) 295
LpnPI CCDG 9 cut(s) 123, 210, 237, 293, 350, 369, 488, 621, 623
Lsp1109I GCAGC 5 cut(s) 425, 442, 496, 508, 677
LweI GCATC 1 cut(s) 652
MaeI CTAG 1 cut(s) 62
MaeIII GTNAC 2 cut(s) 178, 323
MalI GATC 4 cut(s) 77, 151, 231, 335
MboI GATC 4 cut(s) 75, 149, 229, 333
MboII GAAGA 3 cut(s) 224, 577, 664
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 362
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 85
MnlI CCTC 6 cut(s) 66, 153, 163, 303, 367, 402
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 279, 441, 633
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 521, 668
MspI CCGG 2 cut(s) 197, 608
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 3 cut(s) 506, 518, 679
NdeII GATC 4 cut(s) 75, 149, 229, 333
NlaIII CATG 2 cut(s) 290, 677
NlaIV GGNNCC 2 cut(s) 390, 606
NmuCI GTSAC 2 cut(s) 178, 323
NspI RCATGY 2 cut(s) 290, 677
NspV TTCGAA 1 cut(s) 107
PaeI GCATGC 1 cut(s) 290
PfeI GAWTC 5 cut(s) 85, 109, 422, 692, 698
PflMI CCANNNNNTGG 1 cut(s) 496
PkrI GCNGC 6 cut(s) 82, 415, 432, 511, 523, 667
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PpuMI RGGWCCY 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 389
PspN4I GGNNCC 2 cut(s) 390, 606
PspPI GGNCC 4 cut(s) 140, 194, 355, 389
PspPPI RGGWCCY 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 94
PvuII CAGCTG 2 cut(s) 521, 668
RsaI GTAC 4 cut(s) 116, 222, 468, 479
RsaNI GTAC 4 cut(s) 115, 221, 467, 478
SaqAI TTAA 3 cut(s) 279, 441, 633
SatI GCNGC 6 cut(s) 81, 414, 431, 510, 522, 666
Sau3AI GATC 4 cut(s) 75, 149, 229, 333
Sau96I GGNCC 4 cut(s) 140, 194, 355, 389
SchI GAGTC 1 cut(s) 85
SfaNI GCATC 1 cut(s) 652
SfcI CTRYAG 1 cut(s) 417
SfuI TTCGAA 1 cut(s) 107
SinI GGWCC 3 cut(s) 140, 194, 389
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SphI GCATGC 1 cut(s) 290
Sse9I AATT 1 cut(s) 362
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 62
StyI CCWWGG 4 cut(s) 61, 252, 319, 600
TaaI ACNGT 5 cut(s) 10, 157, 166, 187, 568
TaqI TCGA 3 cut(s) 12, 107, 152
TasI AATT 1 cut(s) 362
TatI WGTACW 1 cut(s) 466
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 5 cut(s) 85, 109, 422, 692, 698
Tru1I TTAA 3 cut(s) 279, 441, 633
Tru9I TTAA 3 cut(s) 279, 441, 633
TseFI GTSAC 2 cut(s) 178, 323
TseI GCWGC 5 cut(s) 413, 430, 509, 521, 665
Tsp45I GTSAC 2 cut(s) 178, 323
TspDTI ATGAA 2 cut(s) 276, 395
Van91I CCANNNNNTGG 1 cut(s) 496
VpaK11BI GGWCC 3 cut(s) 140, 194, 389
XapI RAATTY 1 cut(s) 362
XceI RCATGY 2 cut(s) 290, 677
XmaJI CCTAGG 1 cut(s) 61
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.