Rh4BG305400

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011256
Physical Location & Seq
Reverse (-)
47550374 .. 47552343
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG305400.1

Sequence Viewer

Length: 495 bp
ATGGCTACCATCGAGAAGAAGGATTTAGAGGGCAAGGTAGATGAAGCCACGGGCAAGTTTAACAAGCATGCTCCTCCTTTGGCTAAGCAGGTTGCAAGCCAAGGTCACAGTTTGATCCAGACAACTTTGGAGAAGGGCCAAAAATTTGTGAAGGAGGCTCAAACTGGGGGTCCTCGCTCAGCCATTCATTATGCAGCTACAGAATCTAAGCAGCTTGTATTTAACCAATCTGTGAAGCTGTGGGTTGTACTTGACCAGTACCATCCAATCCACAAAGTGGCAGAGAAGGCTGCACCAACAGCTGCTCACTTGTCCGAGAAATACAACCACACCATAAGGGACTTGACCATGAAGGGATATACCATCTTTGGCTATTTGCCCTTGGTTCCGGTTGATGATATTTCCAAAGCAGTTAAGCAGGGTAAGGCTGGGAAGAAGGGAGATGCAGCTGGGCATGTTGAGCATAAATCTGATTCTGATTCTGATTCCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

164

Amino Acids

17.87

Weight (kDa)

8.93

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 10 - 140 6.5e-39 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67360 AT1G67360
fragaria_vesca FvH4_4g23790
malus_domestica MD01G1161800.v1.1 MD08G1165600.v1.1 MD13G1118800.v1.1 MD16G1119000.v1.1
prunus_persica Prupe.1G226000_v2.0.a1
pyrus_communis pycom13g10370 pycom16g10190
rosa_chinensis RchiOBHm_Chr4g0430281
rosa_laevigata RLG00000006988
rosa_multiflora Rmu_sc0001089.1_g000026
rosa_roxburghii Rroxscaffold_5G00372080
rosa_rugosa Rorug04G0242400
rosa_samantha Rh4AG298800 Rh4BG305400 Rh4CG320900 Rh4DG302700
rosa_wichuraiana Rw4G025910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 277
AclWI GGATC 1 cut(s) 109
AcsI RAATTY 1 cut(s) 143
AdeI CACNNNGTG 1 cut(s) 277
AfaI GTAC 2 cut(s) 249, 260
AfiI CCNNNNNNNGG 1 cut(s) 277
AluBI AGCT 5 cut(s) 197, 214, 238, 302, 449
AluI AGCT 5 cut(s) 197, 214, 238, 302, 449
AlwI GGATC 1 cut(s) 109
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 5 cut(s) 194, 211, 290, 302, 446
ApoI RAATTY 1 cut(s) 143
ArsI GACNNNNNNTTYG 2 cut(s) 154, 186
AspS9I GGNCC 2 cut(s) 136, 170
AvaII GGWCC 1 cut(s) 170
BbvI GCAGC 5 cut(s) 206, 223, 277, 289, 458
BccI CCATC 3 cut(s) 17, 270, 371
BfmI CTRYAG 1 cut(s) 198
BfuAI ACCTGC 1 cut(s) 79
BisI GCNGC 5 cut(s) 195, 212, 291, 303, 447
BlpI GCTNAGC 2 cut(s) 84, 178
BlsI GCNGC 5 cut(s) 196, 213, 292, 304, 448
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 136, 170
BmiI GGNNCC 2 cut(s) 171, 387
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 433
BmuI ACTGGG 1 cut(s) 174
Bpu1102I GCTNAGC 2 cut(s) 84, 178
BsaJI CCNNGG 3 cut(s) 48, 100, 381
BsaWI WCCGGW 1 cut(s) 388
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 169, 256
BseDI CCNNGG 3 cut(s) 48, 100, 381
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 2 cut(s) 169, 256
BseRI GAGGAG 1 cut(s) 63
BseXI GCAGC 5 cut(s) 206, 223, 277, 289, 458
BseYI CCCAGC 2 cut(s) 428, 449
BsgI GTGCAG 1 cut(s) 276
BshFI GGCC 1 cut(s) 138
BsiSI CCGG 1 cut(s) 389
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 353
BsnI GGCC 1 cut(s) 138
Bsp143I GATC 1 cut(s) 114
Bsp1720I GCTNAGC 2 cut(s) 84, 178
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 191
BspLI GGNNCC 2 cut(s) 171, 387
BspMI ACCTGC 1 cut(s) 79
BspPI GGATC 1 cut(s) 109
BsrI ACTGG 2 cut(s) 169, 256
BssECI CCNNGG 3 cut(s) 48, 100, 381
BssMI GATC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 100, 381
Bst4CI ACNGT 1 cut(s) 110
BstC8I GCNNGC 2 cut(s) 69, 97
BstDEI CTNAG 3 cut(s) 84, 178, 207
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 262
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 3 cut(s) 287, 299, 460
BstNSI RCATGY 2 cut(s) 71, 458
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 5 cut(s) 206, 223, 277, 289, 458
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 1 cut(s) 262
BveI ACCTGC 1 cut(s) 79
Cac8I GCNNGC 2 cut(s) 69, 97
Cfr13I GGNCC 2 cut(s) 136, 170
Csp6I GTAC 2 cut(s) 248, 259
CviAII CATG 3 cut(s) 68, 349, 455
CviQI GTAC 2 cut(s) 248, 259
DdeI CTNAG 3 cut(s) 84, 178, 207
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
DraIII CACNNNGTG 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 100, 381
Eco47I GGWCC 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 170
EcoT14I CCWWGG 2 cut(s) 100, 381
ErhI CCWWGG 2 cut(s) 100, 381
FaeI CATG 3 cut(s) 71, 352, 458
FaiI YATR 7 cut(s) 69, 192, 335, 350, 360, 456, 465
FaqI GGGAC 1 cut(s) 353
FatI CATG 3 cut(s) 67, 348, 454
Fnu4HI GCNGC 5 cut(s) 195, 212, 291, 303, 447
FokI GGATG 1 cut(s) 249
Fsp4HI GCNGC 5 cut(s) 195, 212, 291, 303, 447
GluI GCNGC 5 cut(s) 195, 212, 291, 303, 447
GsaI CCCAGC 2 cut(s) 432, 453
HaeIII GGCC 1 cut(s) 138
HapII CCGG 1 cut(s) 389
Hin1II CATG 3 cut(s) 71, 352, 458
HinfI GANTC 4 cut(s) 203, 473, 479, 485
HpaII CCGG 1 cut(s) 389
Hpy188I TCNGA 5 cut(s) 316, 472, 478, 484, 490
Hpy188III TCNNGA 2 cut(s) 13, 118
HpyAV CCTTC 6 cut(s) 13, 127, 145, 280, 346, 430
HpyCH4III ACNGT 1 cut(s) 110
HpyCH4V TGCA 4 cut(s) 95, 194, 293, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 299, 460
HpyF3I CTNAG 3 cut(s) 84, 178, 207
Hsp92II CATG 3 cut(s) 71, 352, 458
Kzo9I GATC 1 cut(s) 114
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 8 cut(s) 74, 131, 150, 269, 402, 404, 414, 435
Lsp1109I GCAGC 5 cut(s) 206, 223, 277, 289, 458
LweI GCATC 1 cut(s) 433
MaeIII GTNAC 1 cut(s) 104
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 2 cut(s) 28, 445
MluCI AATT 1 cut(s) 143
MnlI CCTC 4 cut(s) 22, 84, 148, 183
MseI TTAA 3 cut(s) 60, 222, 414
MspA1I CMGCKG 2 cut(s) 302, 449
MspI CCGG 1 cut(s) 389
MwoI GCNNNNNNNGC 3 cut(s) 287, 299, 460
NdeII GATC 1 cut(s) 114
NlaIII CATG 3 cut(s) 71, 352, 458
NlaIV GGNNCC 2 cut(s) 171, 387
NmuCI GTSAC 1 cut(s) 104
NspI RCATGY 2 cut(s) 71, 458
PaeI GCATGC 1 cut(s) 71
PfeI GAWTC 4 cut(s) 203, 473, 479, 485
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 5 cut(s) 196, 213, 292, 304, 448
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
PspFI CCCAGC 2 cut(s) 428, 449
PspN4I GGNNCC 2 cut(s) 171, 387
PspPI GGNCC 2 cut(s) 136, 170
PspPPI RGGWCCY 1 cut(s) 170
PvuII CAGCTG 2 cut(s) 302, 449
RsaI GTAC 2 cut(s) 249, 260
RsaNI GTAC 2 cut(s) 248, 259
SaqAI TTAA 3 cut(s) 60, 222, 414
SatI GCNGC 5 cut(s) 195, 212, 291, 303, 447
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 2 cut(s) 136, 170
SetI ASST 8 cut(s) 39, 93, 106, 199, 216, 240, 304, 451
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 1 cut(s) 170
SphI GCATGC 1 cut(s) 71
Sse9I AATT 1 cut(s) 143
StyI CCWWGG 2 cut(s) 100, 381
TaaI ACNGT 1 cut(s) 110
TaqI TCGA 1 cut(s) 12
TasI AATT 1 cut(s) 143
TatI WGTACW 1 cut(s) 247
TfiI GAWTC 4 cut(s) 203, 473, 479, 485
Tru1I TTAA 3 cut(s) 60, 222, 414
Tru9I TTAA 3 cut(s) 60, 222, 414
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 5 cut(s) 194, 211, 290, 302, 446
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 3 cut(s) 57, 176, 365
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 1 cut(s) 143
XceI RCATGY 2 cut(s) 71, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.