Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0011133

Physical Location & Seq

Genomic Coordinates Forward (+)

61115560 .. 61122416

Gene Structure 6857 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0458300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2457 bp

ATGAACTCCAACCCCGCACACAGTAACAGTAACGGCTACTATGCACAGCCGCCGCAGCAGCACCAGCACCAGCACCAGCAAGGAATGAACAGAGTTCGCCGGGGGCCACGTGGCAGGTCACGTGACGGCCATGGAAGTGACATTATTAGGGTTTCTGAGGCTACCAAGCAGCCGGAAGGAGCGCCTTGCACGGACTTCGACATGGCCTATTTCCACTCCTACGCCCACGTTGGCATCCACGAAGAGATGATCAAGGATCGTGTGCGCACCGAAACTTACAGGGCAGCAATTATGCAGCACCAGAGTTGTATTGCTGGCAAAGTGGTAGTGGACGTTGGCTGTGGCACAGGAATTCTCTCTATCTTTTGTGCTCAAGCTGGTGCAAAACGTGTATATGCTGTCGATGCAAGTGACATTGCAGTGCAGGCAAATGAAGTTGTAAAAGCAAACAATCTATCTGATACCATCATCGTACTACATGGGCGAGTTGAGGATGTTGATATTGATGAGGAGGTTGATGTTATAATTTCAGAGTGGATGGGATACATGCTTCTGTATGAGAGTATGCTTGGAAGTGTCATTACAGCCAGAGATCGCTGGCTAAAACCCGGAGGTCTTATACTTCCTTCAAATGCAACGTTGTACATAGCACCTATCACACATGCTGATAGATACAGTGAAAGCATTGATTTCTGGCGCAATGTTTATGGAATTGATATGTCTGCCATGTTGCCTTTGGCAAAGCAATGTGCGTTTGAAGAGCCATGTGTTGAGACAATCGTGGGTGAGAATGTTTTAACGTGGCCACATGTGGTTAAGCATGTGGACTGCCATAAAATTTCACTTCATGAGCTGGAATCTGTTACAACAAGCTATAAGTTCAAATCAATGATGCGAGCTCCACTACATGGGTTTGCATTTTGGTTTGACGTCGAATTCAATGGACCTGCAATAGCTCTCACAAATAATCATGCACCATCCCAACTTCCTGACAATCATACAGTGGAGGGTCAGCAGAGGAGGAAACGGGCAAATCCCAGTGATGCGTTGGTGCTGTCGACTGCACCTGAGGACCCCCCAACTCATTGGCAACAGACTGTGCTATACTTTTATGACCCAATAGAGGTGGAGCAAGATCAACTGATTGAAGGTTCCGTTACATTATCACAAAGCAAAGAAAATCGTCGATTCATGAATATCCATCTCGAATATGCCTCGGGTGGAAGATCCTTTGTCAAAGAGTCAGTGATGAGCCCCCAACTCTCCGCAGACCTTGATTCTTTACAGATACTTCTGGGTGACCTTGCAATAACTTTTCCTTGCAAAGCCCTGAAGAAAAGGCTACTTATTAGAGACATAGATTCTCTTAAACTTGGGAGGGAGTTCAAGGGGGAAGAAAAGTGCCTTTTCCGAATACATAAGCTCTCCTTTTGCTTGCTTGTGCTTGCTCAATGGATCCGAAGTTATCTTTCTTCCCTTTGTGATGATGACATCCTTCTGGTTTCACCTACAGTCTCATTTTGGCTGGCAAATTCTCAGAATTTGGAGGCTACTTTTAGAGACTTCGAGGAGTCTAATCAACTTGGTGAAAAGCAAGTTGTGATATTCACGGTGAATGATAATCAAGACGTGAGCCAAAATGACGGCGGAAACCATTGGAGTTTGCTGGTGTATTACAGAAAATCAAATGCATTTGTGCATTACGACAGCTTGGGGGGTTTGAATAACTTGGTTGCCAGGAAGTTTTACGGAGCAGTTAAGAAATATGTGGCAGCTGCAGCAACACAAACACCAACTATTGGGACTAGTAGTTTGGTTGCCACCACTAGGCCTATTATCGGCCACAAGTACTACTCTAAGCACTTGCGAATTAGAGATCATCATCATAAGAAGAAGCAGGAGAAGAAGAAAGTATCGGGGCCTTTTGTTATGATGGGGCGCACCTTCTCTAACAAGTTTCATCAATTTAGAAATTATCACAAGAAGAAGAATATACTGGGGGGTTTTGTTAGGGGGCACAGCTTGCTTAAGAAGTGCTTGCGTCAGTATACAAATTATCATCCTACGAAGAATATAAGGACAAGGGATACGACTTGGAAGAAGGTGTGTCATGCCCTGCAAAACTACTTGGTCAATAATAATGGAGATGTTGAAGAGACTGGTACTAGTGATAAGGATGATGATAATGATCCTTGGTTTAGAGAAGAGAATGTTATGCCTCAGCAGACAAATCTGTACGACTGCGGTTTATATGTGATGTCTGTTGCTAGAGTAATCTGTCACTGGTACTACGACGAGGAGGAACAGCAGCACCAGCAGCAGAATTGCTGCTGCTTGGGGGACGACCGGTTTCCCAATATTATGAAGCATCTTGATAACTCTATAGAATACGCAATGCGCCCTCAATTGTTGAGGTTGATTCAACGCCTAAAACGTAATATTCCGTCTTCAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009966 GO:0010033 GO:0010037 GO:0010469 GO:0010646 GO:0015698 GO:0015706 GO:0023051 GO:0030545 GO:0042221 GO:0042493 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0065007 GO:0065009 GO:0071705 GO:0072347 GO:0098772 GO:1901700 GO:1902025 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

818

Amino Acids

93.31

Weight (kDa)

7.41

Isoelectric Point (pI)

48.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_9	PF08003	87 - 210	4.7e-06	Protein of unknown function (DUF1698)
TehB	PF03848	91 - 208	2.5e-06	Tellurite resistance protein TehB
TRM5-TYW2_MTfase	PF02475	94 - 182	3.8e-07	TRM5/TYW2 methyltransferase domain
Methyltransf_23	PF13489	103 - 210	3.5e-06	Methyltransferase domain
PrmA	PF06325	105 - 178	8.7e-13	Ribosomal protein L11 methyltransferase (PrmA)
MTS	PF05175	105 - 177	2.8e-07	Methyltransferase small domain
Methyltransf_31	PF13847	106 - 210	1.2e-06	Methyltransferase domain
Methyltransf_25	PF13649	109 - 205	2.3e-11	Methyltransferase domain
Methyltransf_11	PF08241	110 - 207	1.7e-09	Methyltransferase domain
PRMT_C	PF22528	211 - 316	3.6e-20	Arginine methyltransferase oligomerization subdomain
PRMT_C	PF22528	349 - 406	1.3e-14	Arginine methyltransferase oligomerization subdomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29290
fragaria_vesca	FvH4_3g07300
malus_domestica	MD00G1009200.v1.1 MD00G1221100.v1.1 MD10G1275500.v1.1
prunus_persica	Prupe.4G067000_v2.0.a1
pyrus_communis	pycom05g27420 pycom10g23010
rosa_chinensis	RchiOBHm_Chr5g0011661
rosa_laevigata	RLG00000031852
rosa_multiflora	Rmu_sc0000437.1_g000005 Rmu_sc0006382.1_g000003 Rmu_sc0017389.1_g000001
rosa_roxburghii	Rroxscaffold_1G00064850
rosa_rugosa	Rorug04G0458300
rosa_samantha	Rh5AG090100 Rh5BG085800 Rh5CG099500 Rh5DG085500
rosa_wichuraiana	Rw5G007840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	524
AatII	GACGTC	1 cut(s)	933
Acc16I	TGCGCA	1 cut(s)	266
Acc36I	ACCTGC	2 cut(s)	105, 955
AccB7I	CCANNNNNTGG	2 cut(s)	908, 1799
AccI	GTMKAC	2 cut(s)	1058, 2048
AciI	CCGC	6 cut(s)	15, 50, 53, 1266, 1647, 2244
AclI	AACGTT	1 cut(s)	638
AclWI	GGATC	5 cut(s)	264, 1221, 1450, 1463, 2183
AcoI	YGGCCR	3 cut(s)	127, 803, 1840
AcsI	RAATTY	5 cut(s)	351, 837, 935, 1531, 1540
AcuI	CTGAAG	1 cut(s)	1352
AcvI	CACGTG	2 cut(s)	110, 122
AcyI	GRCGYC	1 cut(s)	930
AfaI	GTAC	6 cut(s)	474, 644, 1850, 2164, 2237, 2288
AfiI	CCNNNNNNNGG	5 cut(s)	908, 1123, 1799, 1827, 1838
AflII	CTTAAG	1 cut(s)	2027
AflIII	ACRYGT	2 cut(s)	388, 808
AgeI	ACCGGT	1 cut(s)	2346
AhlI	ACTAGT	2 cut(s)	1805, 2165
AjiI	CACGTC	1 cut(s)	1630
AjnI	CCWGG	1 cut(s)	1736
AluBI	AGCT	9 cut(s)	377, 853, 873, 899, 956, 1423, 1710, 1775, 2022
AluI	AGCT	9 cut(s)	377, 853, 873, 899, 956, 1423, 1710, 1775, 2022
Alw21I	GWGCWC	2 cut(s)	373, 901
Alw26I	GTCTC	5 cut(s)	767, 1347, 1519, 1554, 2150
AlwI	GGATC	5 cut(s)	264, 1221, 1450, 1463, 2183
Ama87I	CYCGRG	1 cut(s)	1216
AoxI	GGCC	7 cut(s)	104, 127, 204, 803, 1829, 1840, 1919
ApoI	RAATTY	5 cut(s)	351, 837, 935, 1531, 1540
ArsI	GACNNNNNNTTYG	2 cut(s)	1795, 1827
AsiGI	ACCGGT	1 cut(s)	2346
AspLEI	GCGC	5 cut(s)	184, 267, 699, 1941, 2400
AspS9I	GGNCC	4 cut(s)	104, 944, 1072, 1919
AsuC2I	CCSGG	2 cut(s)	101, 609
AsuHPI	GGTGA	5 cut(s)	797, 1310, 1497, 1598, 1624
AvaI	CYCGRG	1 cut(s)	1216
AvaII	GGWCC	2 cut(s)	944, 1072
AxyI	CCTNAGG	1 cut(s)	1068
BaeGI	GKGCMC	1 cut(s)	2019
BalI	TGGCCA	1 cut(s)	805
BamHI	GGATCC	1 cut(s)	1455
BanII	GRGCYC	2 cut(s)	901, 1256
BarI	GAAGNNNNNNTAC	2 cut(s)	565, 597
BbrPI	CACGTG	2 cut(s)	110, 122
BbsI	GAAGAC	1 cut(s)	2439
Bbv12I	GWGCWC	2 cut(s)	373, 901
BbvCI	CCTCAGC	1 cut(s)	2220
BccI	CCATC	5 cut(s)	473, 532, 985, 1209, 1927
BceAI	ACGGC	3 cut(s)	49, 142, 1660
BcgI	CGANNNNNNTGC	2 cut(s)	178, 212
BciT130I	CCWGG	1 cut(s)	1738
BciVI	GTATCC	2 cut(s)	536, 2080
BclI	TGATCA	1 cut(s)	249
BcnI	CCSGG	2 cut(s)	101, 609
BcoDI	GTCTC	5 cut(s)	767, 1347, 1519, 1554, 2150
BcuI	ACTAGT	2 cut(s)	1805, 2165
BfaI	CTAG	4 cut(s)	1806, 1827, 2166, 2268
BfmI	CTRYAG	3 cut(s)	1509, 1776, 2382
BfoI	RGCGCY	1 cut(s)	185
BfrI	CTTAAG	1 cut(s)	2027
BfuAI	ACCTGC	2 cut(s)	105, 955
BfuI	GTATCC	2 cut(s)	536, 2080
BmcAI	AGTACT	1 cut(s)	1850
Bme1390I	CCNGG	3 cut(s)	101, 609, 1738
Bme18I	GGWCC	2 cut(s)	944, 1072
BmeT110I	CYCGRG	1 cut(s)	1216
BmgBI	CACGTC	1 cut(s)	1630
BmgT120I	GGNCC	4 cut(s)	104, 944, 1072, 1919
BmiI	GGNNCC	5 cut(s)	105, 1074, 1153, 1457, 1920
BmrFI	CCNGG	3 cut(s)	101, 609, 1738
BmrI	ACTGGG	2 cut(s)	1032, 2006
BmsI	GCATC	5 cut(s)	243, 394, 882, 1033, 2377
BmuI	ACTGGG	2 cut(s)	1032, 2006
BpiI	GAAGAC	1 cut(s)	2439
Bpu10I	CCTNAGC	1 cut(s)	2220
BpuEI	CTTGAG	1 cut(s)	357
BpuMI	CCSGG	2 cut(s)	101, 609
BsaAI	YACGTR	2 cut(s)	110, 122
BsaBI	GATNNNNATC	2 cut(s)	1881, 2187
BsaHI	GRCGYC	1 cut(s)	930
BsaJI	CCNNGG	4 cut(s)	100, 130, 1215, 2192
BsaWI	WCCGGW	1 cut(s)	2346
Bsc4I	CCNNNNNNNGG	5 cut(s)	908, 1123, 1799, 1827, 1838
Bse118I	RCCGGY	1 cut(s)	2346
Bse1I	ACTGG	4 cut(s)	1038, 2001, 2164, 2288
Bse21I	CCTNAGG	1 cut(s)	1068
Bse3DI	GCAATG	4 cut(s)	414, 706, 752, 2400
Bse8I	GATNNNNATC	2 cut(s)	1881, 2187
BseBI	CCWGG	1 cut(s)	1738
BseDI	CCNNGG	4 cut(s)	100, 130, 1215, 2192
BseGI	GGATG	7 cut(s)	234, 499, 543, 977, 1491, 2059, 2182
BseJI	GATNNNNATC	2 cut(s)	1881, 2187
BseLI	CCNNNNNNNGG	5 cut(s)	908, 1123, 1799, 1827, 1838
BseMI	GCAATG	4 cut(s)	414, 706, 752, 2400
BseMII	CTCAG	4 cut(s)	147, 1059, 1550, 2234
BseNI	ACTGG	4 cut(s)	1038, 2001, 2164, 2288
BseRI	GAGGAG	4 cut(s)	524, 1033, 1583, 2312
BseSI	GKGCMC	1 cut(s)	2019
BsgI	GTGCAG	2 cut(s)	443, 1047
Bsh1285I	CGRYCG	1 cut(s)	2347
BshFI	GGCC	7 cut(s)	106, 129, 206, 805, 1831, 1842, 1921
BshTI	ACCGGT	1 cut(s)	2346
BsiEI	CGRYCG	1 cut(s)	2347
BsiHKAI	GWGCWC	2 cut(s)	373, 901
BsiHKCI	CYCGRG	1 cut(s)	1216
BsiSI	CCGG	4 cut(s)	100, 173, 609, 2347
BslFI	GGGAC	2 cut(s)	1816, 2354
BslI	CCNNNNNNNGG	5 cut(s)	908, 1123, 1799, 1827, 1838
BsmAI	GTCTC	5 cut(s)	767, 1347, 1519, 1554, 2150
BsmFI	GGGAC	2 cut(s)	1816, 2354
BsnI	GGCC	7 cut(s)	106, 129, 206, 805, 1831, 1842, 1921
BsoBI	CYCGRG	1 cut(s)	1216
Bsp1286I	GDGCHC	4 cut(s)	373, 901, 1256, 2019
Bsp1407I	TGTACA	1 cut(s)	642
Bsp143I	GATC	8 cut(s)	249, 256, 592, 1135, 1226, 1455, 1876, 2188
Bsp19I	CCATGG	1 cut(s)	130
BspACI	CCGC	6 cut(s)	15, 50, 53, 1266, 1647, 2244
BspANI	GGCC	7 cut(s)	106, 129, 206, 805, 1831, 1842, 1921
BspCNI	CTCAG	4 cut(s)	148, 1060, 1549, 2233
BspHI	TCATGA	2 cut(s)	847, 1191
BspLI	GGNNCC	5 cut(s)	105, 1074, 1153, 1457, 1920
BspMAI	CTGCAG	1 cut(s)	1780
BspMI	ACCTGC	2 cut(s)	105, 955
BspPI	GGATC	5 cut(s)	264, 1221, 1450, 1463, 2183
BspQI	GCTCTTC	1 cut(s)	753
BspTI	CTTAAG	1 cut(s)	2027
BsrDI	GCAATG	4 cut(s)	414, 706, 752, 2400
BsrFI	RCCGGY	1 cut(s)	2346
BsrGI	TGTACA	1 cut(s)	642
BsrI	ACTGG	4 cut(s)	1038, 2001, 2164, 2288
BssAI	RCCGGY	1 cut(s)	2346
BssECI	CCNNGG	4 cut(s)	100, 130, 1215, 2192
BssMI	GATC	8 cut(s)	249, 256, 592, 1135, 1226, 1455, 1876, 2188
BssNAI	GTATAC	1 cut(s)	2049
BssNI	GRCGYC	1 cut(s)	930
BssT1I	CCWWGG	2 cut(s)	130, 2192
Bst1107I	GTATAC	1 cut(s)	2049
Bst2UI	CCWGG	1 cut(s)	1738
Bst4CI	ACNGT	7 cut(s)	23, 29, 677, 1003, 1099, 1513, 1612
Bst6I	CTCTTC	4 cut(s)	237, 753, 2148, 2199
BstACI	GRCGYC	1 cut(s)	930
BstAFI	CTTAAG	1 cut(s)	2027
BstAPI	GCANNNNNTGC	1 cut(s)	2023
BstAUI	TGTACA	1 cut(s)	642
BstBAI	YACGTR	2 cut(s)	110, 122
BstC8I	GCNNGC	9 cut(s)	316, 426, 599, 897, 1436, 1446, 1527, 2024, 2039
BstDEI	CTNAG	5 cut(s)	156, 1068, 1536, 1857, 2220
BstDSI	CCRYGG	1 cut(s)	130
BstEII	GGTNACC	1 cut(s)	1298
BstF5I	GGATG	7 cut(s)	234, 499, 543, 977, 1491, 2059, 2182
BstH2I	RGCGCY	1 cut(s)	185
BstHHI	GCGC	5 cut(s)	184, 267, 699, 1941, 2400
BstKTI	GATC	8 cut(s)	252, 259, 595, 1138, 1229, 1458, 1879, 2191
BstMAI	GTCTC	5 cut(s)	767, 1347, 1519, 1554, 2150
BstMBI	GATC	8 cut(s)	249, 256, 592, 1135, 1226, 1455, 1876, 2188
BstMCI	CGRYCG	1 cut(s)	2347
BstMWI	GCNNNNNNNGC	9 cut(s)	55, 58, 64, 404, 425, 1778, 2023, 2314, 2317
BstNI	CCWGG	1 cut(s)	1738
BstNSI	RCATGY	4 cut(s)	550, 665, 812, 824
BstPI	GGTNACC	1 cut(s)	1298
BstSCI	CCNGG	3 cut(s)	99, 607, 1736
BstSFI	CTRYAG	3 cut(s)	1509, 1776, 2382
BstSLI	GKGCMC	1 cut(s)	2019
BstV2I	GAAGAC	1 cut(s)	2439
BstX2I	RGATCY	2 cut(s)	1226, 1455
BstXI	CCANNNNNNTGG	1 cut(s)	1086
BstYI	RGATCY	2 cut(s)	1226, 1455
BstZ17I	GTATAC	1 cut(s)	2049
Bsu36I	CCTNAGG	1 cut(s)	1068
BsuI	GTATCC	2 cut(s)	536, 2080
BsuRI	GGCC	7 cut(s)	106, 129, 206, 805, 1831, 1842, 1921
BtgI	CCRYGG	1 cut(s)	130
BtrI	CACGTC	1 cut(s)	1630
BtsCI	GGATG	7 cut(s)	234, 499, 543, 977, 1491, 2059, 2182
BtsI	GCAGTG	1 cut(s)	426
BtsIMutI	CAGTG	6 cut(s)	426, 682, 1008, 1045, 1251, 2281
BveI	ACCTGC	2 cut(s)	105, 955
Cac8I	GCNNGC	9 cut(s)	316, 426, 599, 897, 1436, 1446, 1527, 2024, 2039
CciI	TCATGA	2 cut(s)	847, 1191
CfoI	GCGC	5 cut(s)	184, 267, 699, 1941, 2400
Cfr10I	RCCGGY	1 cut(s)	2346
Cfr13I	GGNCC	4 cut(s)	104, 944, 1072, 1919
CseI	GACGC	1 cut(s)	2030
Csp6I	GTAC	6 cut(s)	473, 643, 1849, 2163, 2236, 2287
CspAI	ACCGGT	1 cut(s)	2346
CspCI	CAANNNNNGTGG	2 cut(s)	1107, 1142
CviQI	GTAC	6 cut(s)	473, 643, 1849, 2163, 2236, 2287
DdeI	CTNAG	5 cut(s)	156, 1068, 1536, 1857, 2220
DpnI	GATC	8 cut(s)	251, 258, 594, 1137, 1228, 1457, 1878, 2190
DpnII	GATC	8 cut(s)	249, 256, 592, 1135, 1226, 1455, 1876, 2188
EaeI	YGGCCR	3 cut(s)	127, 803, 1840
Eam1104I	CTCTTC	4 cut(s)	237, 753, 2148, 2199
EarI	CTCTTC	4 cut(s)	237, 753, 2148, 2199
EciI	GGCGGA	1 cut(s)	1662
Ecl136II	GAGCTC	1 cut(s)	899
Eco130I	CCWWGG	2 cut(s)	130, 2192
Eco147I	AGGCCT	1 cut(s)	1831
Eco24I	GRGCYC	2 cut(s)	901, 1256
Eco47I	GGWCC	2 cut(s)	944, 1072
Eco53kI	GAGCTC	1 cut(s)	899
Eco57I	CTGAAG	1 cut(s)	1352
Eco72I	CACGTG	2 cut(s)	110, 122
Eco81I	CCTNAGG	1 cut(s)	1068
Eco88I	CYCGRG	1 cut(s)	1216
Eco91I	GGTNACC	1 cut(s)	1298
EcoICRI	GAGCTC	1 cut(s)	899
EcoO109I	RGGNCCY	2 cut(s)	1072, 1919
EcoO65I	GGTNACC	1 cut(s)	1298
EcoRI	GAATTC	2 cut(s)	351, 935
EcoRII	CCWGG	1 cut(s)	1736
EcoT14I	CCWWGG	2 cut(s)	130, 2192
EcoT22I	ATGCAT	1 cut(s)	1693
EcoT38I	GRGCYC	2 cut(s)	901, 1256
ErhI	CCWWGG	2 cut(s)	130, 2192
FalI	AAGNNNNNCTT	4 cut(s)	1412, 1444, 2021, 2053
FaqI	GGGAC	2 cut(s)	1816, 2354
FauI	CCCGC	1 cut(s)	22
FbaI	TGATCA	1 cut(s)	249
FblI	GTMKAC	2 cut(s)	1058, 2048
FokI	GGATG	7 cut(s)	221, 506, 550, 964, 1478, 2046, 2189
FriOI	GRGCYC	2 cut(s)	901, 1256
FspAI	RTGCGCAY	1 cut(s)	266
FspBI	CTAG	4 cut(s)	1806, 1827, 2166, 2268
FspI	TGCGCA	1 cut(s)	266
GlaI	GCGC	5 cut(s)	183, 266, 698, 1940, 2399
HaeII	RGCGCY	1 cut(s)	185
HaeIII	GGCC	7 cut(s)	106, 129, 206, 805, 1831, 1842, 1921
HapII	CCGG	4 cut(s)	100, 173, 609, 2347
HgaI	GACGC	1 cut(s)	2030
HhaI	GCGC	5 cut(s)	184, 267, 699, 1941, 2400
Hin1I	GRCGYC	1 cut(s)	930
Hin6I	GCGC	5 cut(s)	182, 265, 697, 1939, 2398
HinP1I	GCGC	5 cut(s)	182, 265, 697, 1939, 2398
HincII	GTYRAC	1 cut(s)	1059
HindII	GTYRAC	1 cut(s)	1059
HinfI	GANTC	7 cut(s)	857, 1188, 1241, 1277, 1361, 1571, 2419
HpaII	CCGG	4 cut(s)	100, 173, 609, 2347
HphI	GGTGA	5 cut(s)	797, 1310, 1497, 1598, 1624
Hpy166II	GTNNAC	4 cut(s)	331, 826, 1059, 2049
Hpy188I	TCNGA	6 cut(s)	157, 460, 532, 1412, 1460, 1539
Hpy188III	TCNNGA	6 cut(s)	848, 989, 1192, 1205, 1625, 2372
Hpy8I	GTNNAC	4 cut(s)	331, 826, 1059, 2049
Hpy99I	CGWCG	3 cut(s)	935, 1188, 2297
HpyAV	CCTTC	6 cut(s)	170, 636, 1142, 1505, 1954, 2095
HpyCH4III	ACNGT	7 cut(s)	23, 29, 677, 1003, 1099, 1513, 1612
HpyF10VI	GCNNNNNNNGC	9 cut(s)	55, 58, 64, 404, 425, 1778, 2023, 2314, 2317
HpyF3I	CTNAG	5 cut(s)	156, 1068, 1536, 1857, 2220
Hsp92I	GRCGYC	1 cut(s)	930
HspAI	GCGC	5 cut(s)	182, 265, 697, 1939, 2398
Ksp22I	TGATCA	1 cut(s)	249
Kzo9I	GATC	8 cut(s)	249, 256, 592, 1135, 1226, 1455, 1876, 2188
LguI	GCTCTTC	1 cut(s)	753
LmnI	GCTCC	4 cut(s)	179, 904, 1129, 1751
LweI	GCATC	5 cut(s)	243, 394, 882, 1033, 2377
MaeI	CTAG	4 cut(s)	1806, 1827, 2166, 2268
MalI	GATC	8 cut(s)	251, 258, 594, 1137, 1228, 1457, 1878, 2190
MboI	GATC	8 cut(s)	249, 256, 592, 1135, 1226, 1455, 1876, 2188
MfeI	CAATTG	1 cut(s)	2405
MflI	RGATCY	2 cut(s)	1226, 1455
MhlI	GDGCHC	4 cut(s)	373, 901, 1256, 2019
MlsI	TGGCCA	1 cut(s)	805
MluNI	TGGCCA	1 cut(s)	805
MlyI	GAGTC	2 cut(s)	1250, 1580
MmeI	TCCRAC	1 cut(s)	33
Mox20I	TGGCCA	1 cut(s)	805
Mph1103I	ATGCAT	1 cut(s)	1693
MscI	TGGCCA	1 cut(s)	805
MseI	TTAA	5 cut(s)	797, 816, 1368, 1758, 2028
MslI	CAYNNNNRTG	2 cut(s)	135, 419
Msp20I	TGGCCA	1 cut(s)	805
MspA1I	CMGCKG	1 cut(s)	1775
MspCI	CTTAAG	1 cut(s)	2027
MspI	CCGG	4 cut(s)	100, 173, 609, 2347
MspR9I	CCNGG	3 cut(s)	101, 609, 1738
MunI	CAATTG	1 cut(s)	2405
MvaI	CCWGG	1 cut(s)	1738
MwoI	GCNNNNNNNGC	9 cut(s)	55, 58, 64, 404, 425, 1778, 2023, 2314, 2317
NciI	CCSGG	2 cut(s)	101, 609
NcoI	CCATGG	1 cut(s)	130
NdeII	GATC	8 cut(s)	249, 256, 592, 1135, 1226, 1455, 1876, 2188
NlaIV	GGNNCC	5 cut(s)	105, 1074, 1153, 1457, 1920
NmuCI	GTSAC	6 cut(s)	117, 122, 137, 410, 1298, 2279
NsbI	TGCGCA	1 cut(s)	266
NsiI	ATGCAT	1 cut(s)	1693
NspI	RCATGY	4 cut(s)	550, 665, 812, 824
PagI	TCATGA	2 cut(s)	847, 1191
PceI	AGGCCT	1 cut(s)	1831
PciI	ACATGT	1 cut(s)	808
PciSI	GCTCTTC	1 cut(s)	753
PcsI	WCGNNNNNNNCGW	1 cut(s)	2431
PfeI	GAWTC	5 cut(s)	857, 1188, 1277, 1361, 2419
PflMI	CCANNNNNTGG	2 cut(s)	908, 1799
PinAI	ACCGGT	1 cut(s)	2346
PleI	GAGTC	2 cut(s)	1249, 1579
PmaCI	CACGTG	2 cut(s)	110, 122
PmlI	CACGTG	2 cut(s)	110, 122
PpsI	GAGTC	2 cut(s)	1249, 1579
Ppu21I	YACGTR	2 cut(s)	110, 122
PpuMI	RGGWCCY	1 cut(s)	1072
PscI	ACATGT	1 cut(s)	808
PsiI	TTATAA	1 cut(s)	524
Psp124BI	GAGCTC	1 cut(s)	901
Psp1406I	AACGTT	1 cut(s)	638
Psp5II	RGGWCCY	1 cut(s)	1072
Psp6I	CCWGG	1 cut(s)	1736
PspCI	CACGTG	2 cut(s)	110, 122
PspEI	GGTNACC	1 cut(s)	1298
PspGI	CCWGG	1 cut(s)	1736
PspN4I	GGNNCC	5 cut(s)	105, 1074, 1153, 1457, 1920
PspPI	GGNCC	4 cut(s)	104, 944, 1072, 1919
PspPPI	RGGWCCY	1 cut(s)	1072
PstI	CTGCAG	1 cut(s)	1780
PsuI	RGATCY	2 cut(s)	1226, 1455
PvuII	CAGCTG	1 cut(s)	1775
RsaI	GTAC	6 cut(s)	474, 644, 1850, 2164, 2237, 2288
RsaNI	GTAC	6 cut(s)	473, 643, 1849, 2163, 2236, 2287
RseI	CAYNNNNRTG	2 cut(s)	135, 419
SacI	GAGCTC	1 cut(s)	901
SalI	GTCGAC	1 cut(s)	1057
SapI	GCTCTTC	1 cut(s)	753
SaqAI	TTAA	5 cut(s)	797, 816, 1368, 1758, 2028
Sau3AI	GATC	8 cut(s)	249, 256, 592, 1135, 1226, 1455, 1876, 2188
Sau96I	GGNCC	4 cut(s)	104, 944, 1072, 1919
ScaI	AGTACT	1 cut(s)	1850
SchI	GAGTC	2 cut(s)	1250, 1580
ScrFI	CCNGG	3 cut(s)	101, 609, 1738
SduI	GDGCHC	4 cut(s)	373, 901, 1256, 2019
SfaNI	GCATC	5 cut(s)	243, 394, 882, 1033, 2377
SfcI	CTRYAG	3 cut(s)	1509, 1776, 2382
SinI	GGWCC	2 cut(s)	944, 1072
SmiMI	CAYNNNNRTG	2 cut(s)	135, 419
SmlI	CTYRAG	2 cut(s)	372, 2027
SmoI	CTYRAG	2 cut(s)	372, 2027
SpeI	ACTAGT	2 cut(s)	1805, 2165
SseBI	AGGCCT	1 cut(s)	1831
SsiI	CCGC	6 cut(s)	15, 50, 53, 1266, 1647, 2244
SspI	AATATT	2 cut(s)	2359, 2440
SspMI	CTAG	4 cut(s)	1806, 1827, 2166, 2268
SstI	GAGCTC	1 cut(s)	901
StuI	AGGCCT	1 cut(s)	1831
StyD4I	CCNGG	3 cut(s)	99, 607, 1736
StyI	CCWWGG	2 cut(s)	130, 2192
TaaI	ACNGT	7 cut(s)	23, 29, 677, 1003, 1099, 1513, 1612
TaqI	TCGA	7 cut(s)	198, 402, 933, 1058, 1186, 1206, 1566
TatI	WGTACW	2 cut(s)	642, 1848
TauI	GCSGC	2 cut(s)	52, 55
TfiI	GAWTC	5 cut(s)	857, 1188, 1277, 1361, 2419
Tru1I	TTAA	5 cut(s)	797, 816, 1368, 1758, 2028
Tru9I	TTAA	5 cut(s)	797, 816, 1368, 1758, 2028
TscAI	CASTG	6 cut(s)	426, 682, 1008, 1045, 1251, 2288
TseFI	GTSAC	6 cut(s)	117, 122, 137, 410, 1298, 2279
Tsp45I	GTSAC	6 cut(s)	117, 122, 137, 410, 1298, 2279
TspDTI	ATGAA	8 cut(s)	17, 101, 447, 836, 1180, 1208, 1949, 2378
TspGWI	ACGGA	4 cut(s)	206, 1144, 1764, 2433
TspRI	CASTG	6 cut(s)	426, 682, 1008, 1045, 1251, 2288
Van91I	CCANNNNNTGG	2 cut(s)	908, 1799
Vha464I	CTTAAG	1 cut(s)	2027
VpaK11BI	GGWCC	2 cut(s)	944, 1072
XapI	RAATTY	5 cut(s)	351, 837, 935, 1531, 1540
XceI	RCATGY	4 cut(s)	550, 665, 812, 824
XcmI	CCANNNNNNNNNTGG	2 cut(s)	733, 1045
XmiI	GTMKAC	2 cut(s)	1058, 2048
XspI	CTAG	4 cut(s)	1806, 1827, 2166, 2268
ZraI	GACGTC	1 cut(s)	931
ZrmI	AGTACT	1 cut(s)	1850
Zsp2I	ATGCAT	1 cut(s)	1693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.