Rorug05G0329700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012933
Physical Location & Seq
Reverse (-)
39708237 .. 39713613
5377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0329700.1

Sequence Viewer

Length: 663 bp
ATGGCGATTCTCTACGCCGTTGTTGCTCGCGGCACCGTCGTCTTGTCCGAGTTTAGCGCCGTCAGCGGAAACACAGGCGCCGTGGCTCGCCGGATCTTAGAGAAGCTTCCGCCCGACTCCGATTCCAGGGTTTGCTTCTCCCAGGATCGCTACATCTTCCACATACTCACCTTGGATGGCCTCACTTTTCTCTGCATGGCCAACGACACTTTCGGCAGGAGGATACCGTTTGCGTACTTGGAGGATATTCATATGAGATTCATGAAAAACTACGGCAGAGTTGCGCCTTATGCGCCTGCTTATGCGATGAACGACGAGTTTTCGAGGGTGTTGCATCAGCAAATGGAGTTTTTCTCCAGCAATCCCAGTGCCGATACTCTCAGCCGCGTGAGAGGGGAAGTCGGTGAGATACGAACTATAATGGTCGACAACATAGAAAAGATATTGGAGAGAGGCGACCGAATCGAACTTCTTGTTGACAAAACTGCAACTATGCAAGACAGTGCATTTCACTTCAAGAAACAGTCAAAACGCCTTCATCGAGCTCTGTGGATGAAAAATGCCAAACTCTTGGCCCTGTTGACGTGCTTGATTGTTGTGTTCCTGTACATTATCATTGCTGCTTGTTGTGGAGGAATCACACTACCGTCCTGCAGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

24.87

Weight (kDa)

8.93

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 29 - 108 5.2e-23 Regulated-SNARE-like domain
Synaptobrevin PF00957 127 - 206 3.3e-27 Synaptobrevin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012933)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60400 AT5G60400 AT5G60400
fragaria_vesca FvH4_3g33040
malus_domestica MD03G1112500.v1.1 MD11G1131400.v1.1
prunus_persica Prupe.6G095800_v2.0.a1 Prupe.6G096000_v2.0.a1
pyrus_communis pycom11g10860
rosa_chinensis RchiOBHm_Chr5g0060411
rosa_multiflora Rmu_sc0003270.1_g000013
rosa_roxburghii Rroxscaffold_1G00020420
rosa_rugosa Rorug05G0329700
rosa_samantha Rh5AG394400 Rh5BG406600 Rh5CG430400 Rh5DG421700
rosa_wichuraiana Rw5G037100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 32, 77
AccI GTMKAC 1 cut(s) 426
AccII CGCG 2 cut(s) 30, 387
AciI CCGC 4 cut(s) 30, 66, 110, 385
AclWI GGATC 2 cut(s) 101, 153
AcoI YGGCCR 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 2 cut(s) 236, 608
AfiI CCNNNNNNNGG 1 cut(s) 126
AgsI TTSAA 1 cut(s) 517
AjiI CACGTC 1 cut(s) 585
AjnI CCWGG 2 cut(s) 125, 141
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 2 cut(s) 106, 545
AluI AGCT 2 cut(s) 106, 545
Alw21I GWGCWC 1 cut(s) 547
AlwI GGATC 2 cut(s) 101, 153
AoxI GGCC 3 cut(s) 178, 198, 573
ApeKI GCWGC 1 cut(s) 620
AspLEI GCGC 4 cut(s) 59, 80, 286, 295
AspS9I GGNCC 1 cut(s) 574
AsuHPI GGTGA 2 cut(s) 160, 416
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 2 cut(s) 32, 77
BanII GRGCYC 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 1 cut(s) 607
BccI CCATC 1 cut(s) 170
BceAI ACGGC 4 cut(s) 2, 44, 65, 289
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 2 cut(s) 127, 143
BciVI GTATCC 1 cut(s) 216
BfmI CTRYAG 1 cut(s) 652
BfoI RGCGCY 2 cut(s) 60, 81
BfuI GTATCC 1 cut(s) 216
BisI GCNGC 3 cut(s) 31, 385, 621
BlsI GCNGC 3 cut(s) 32, 386, 622
Bme1390I CCNGG 2 cut(s) 127, 143
BmgBI CACGTC 1 cut(s) 585
BmgT120I GGNCC 1 cut(s) 574
BmiI GGNNCC 2 cut(s) 34, 79
BmrFI CCNGG 2 cut(s) 127, 143
BmrI ACTGGG 1 cut(s) 360
BmsI GCATC 1 cut(s) 343
BmuI ACTGGG 1 cut(s) 360
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 1 cut(s) 340
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 4 cut(s) 81, 126, 141, 171
Bsc4I CCNNNNNNNGG 1 cut(s) 126
Bse1I ACTGG 1 cut(s) 366
Bse3DI GCAATG 1 cut(s) 615
BseBI CCWGG 2 cut(s) 127, 143
BseDI CCNNGG 4 cut(s) 81, 126, 141, 171
BseGI GGATG 2 cut(s) 181, 558
BseLI CCNNNNNNNGG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 1 cut(s) 394
BseNI ACTGG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 607
Bsh1236I CGCG 2 cut(s) 30, 387
Bsh1285I CGRYCG 1 cut(s) 460
BshFI GGCC 3 cut(s) 180, 200, 575
BshNI GGYRCC 2 cut(s) 32, 77
BsiEI CGRYCG 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 1 cut(s) 91
BslI CCNNNNNNNGG 1 cut(s) 126
BsnI GGCC 3 cut(s) 180, 200, 575
Bsp1286I GDGCHC 1 cut(s) 547
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 3 cut(s) 93, 145, 656
BspACI CCGC 4 cut(s) 30, 66, 110, 385
BspANI GGCC 3 cut(s) 180, 200, 575
BspCNI CTCAG 1 cut(s) 393
BspFNI CGCG 2 cut(s) 30, 387
BspHI TCATGA 2 cut(s) 261, 659
BspLI GGNNCC 2 cut(s) 34, 79
BspMAI CTGCAG 1 cut(s) 656
BspPI GGATC 2 cut(s) 101, 153
BspT107I GGYRCC 2 cut(s) 32, 77
BsrDI GCAATG 1 cut(s) 615
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 1 cut(s) 366
BssECI CCNNGG 4 cut(s) 81, 126, 141, 171
BssMI GATC 3 cut(s) 93, 145, 656
BssNI GRCGYC 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 127, 143
Bst4CI ACNGT 5 cut(s) 37, 228, 503, 525, 648
BstACI GRCGYC 1 cut(s) 78
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 3 cut(s) 28, 88, 297
BstDEI CTNAG 2 cut(s) 97, 380
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 181, 558
BstFNI CGCG 2 cut(s) 30, 387
BstH2I RGCGCY 2 cut(s) 60, 81
BstHHI GCGC 4 cut(s) 59, 80, 286, 295
BstKTI GATC 3 cut(s) 96, 148, 659
BstMBI GATC 3 cut(s) 93, 145, 656
BstMCI CGRYCG 1 cut(s) 460
BstMWI GCNNNNNNNGC 4 cut(s) 23, 63, 290, 292
BstNI CCWGG 2 cut(s) 127, 143
BstSCI CCNGG 2 cut(s) 125, 141
BstSFI CTRYAG 1 cut(s) 652
BstUI CGCG 2 cut(s) 30, 387
BstV1I GCAGC 1 cut(s) 607
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 571
BstYI RGATCY 1 cut(s) 93
BsuI GTATCC 1 cut(s) 216
BsuRI GGCC 3 cut(s) 180, 200, 575
BtgI CCRYGG 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 320
BtrI CACGTC 1 cut(s) 585
BtsCI GGATG 2 cut(s) 181, 558
BtsIMutI CAGTG 2 cut(s) 373, 508
Cac8I GCNNGC 3 cut(s) 28, 88, 297
CciI TCATGA 2 cut(s) 261, 659
CfoI GCGC 4 cut(s) 59, 80, 286, 295
Cfr13I GGNCC 1 cut(s) 574
Csp6I GTAC 2 cut(s) 235, 607
CviAII CATG 3 cut(s) 196, 262, 660
CviJI RGCY 7 cut(s) 86, 106, 180, 200, 384, 545, 575
CviKI_1 RGCY 7 cut(s) 86, 106, 180, 200, 384, 545, 575
CviQI GTAC 2 cut(s) 235, 607
DdeI CTNAG 2 cut(s) 97, 380
DinI GGCGCC 1 cut(s) 79
DpnI GATC 3 cut(s) 95, 147, 658
DpnII GATC 3 cut(s) 93, 145, 656
EaeI YGGCCR 1 cut(s) 198
EciI GGCGGA 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 125, 141
EcoT14I CCWWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 547
EgeI GGCGCC 1 cut(s) 79
EheI GGCGCC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 3 cut(s) 199, 265, 663
FatI CATG 3 cut(s) 195, 261, 659
FauNDI CATATG 1 cut(s) 252
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 31, 385, 621
FokI GGATG 2 cut(s) 188, 565
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 3 cut(s) 31, 385, 621
GlaI GCGC 4 cut(s) 58, 79, 285, 294
GluI GCNGC 3 cut(s) 31, 385, 621
GsuI CTGGAG 1 cut(s) 340
HaeII RGCGCY 2 cut(s) 60, 81
HaeIII GGCC 3 cut(s) 180, 200, 575
HapII CCGG 1 cut(s) 91
HhaI GCGC 4 cut(s) 59, 80, 286, 295
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 3 cut(s) 199, 265, 663
Hin6I GCGC 4 cut(s) 57, 78, 284, 293
HinP1I GCGC 4 cut(s) 57, 78, 284, 293
HincII GTYRAC 3 cut(s) 427, 478, 582
HindII GTYRAC 3 cut(s) 427, 478, 582
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 6 cut(s) 7, 116, 122, 258, 462, 636
HpaII CCGG 1 cut(s) 91
HphI GGTGA 2 cut(s) 160, 416
Hpy166II GTNNAC 3 cut(s) 427, 478, 582
Hpy188I TCNGA 2 cut(s) 49, 121
Hpy188III TCNNGA 3 cut(s) 262, 517, 660
Hpy8I GTNNAC 3 cut(s) 427, 478, 582
Hpy99I CGWCG 2 cut(s) 41, 317
HpyAV CCTTC 1 cut(s) 545
HpyCH4III ACNGT 5 cut(s) 37, 228, 503, 525, 648
HpyCH4IV ACGT 1 cut(s) 584
HpyCH4V TGCA 6 cut(s) 195, 334, 488, 496, 506, 654
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 63, 290, 292
HpyF3I CTNAG 2 cut(s) 97, 380
HpySE526I ACGT 1 cut(s) 584
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 3 cut(s) 199, 265, 663
HspAI GCGC 4 cut(s) 57, 78, 284, 293
KasI GGCGCC 1 cut(s) 77
Kzo9I GATC 3 cut(s) 93, 145, 656
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 1 cut(s) 343
MaeII ACGT 1 cut(s) 584
MalI GATC 3 cut(s) 95, 147, 658
MboI GATC 3 cut(s) 93, 145, 656
MboII GAAGA 1 cut(s) 148
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 547
MlsI TGGCCA 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 200
Mly113I GGCGCC 1 cut(s) 78
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 7 cut(s) 191, 213, 235, 318, 386, 446, 626
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
Msp20I TGGCCA 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 1 cut(s) 91
MspR9I CCNGG 2 cut(s) 127, 143
MvaI CCWGG 2 cut(s) 127, 143
MvnI CGCG 2 cut(s) 30, 387
MwoI GCNNNNNNNGC 4 cut(s) 23, 63, 290, 292
NarI GGCGCC 1 cut(s) 78
NdeI CATATG 1 cut(s) 252
NdeII GATC 3 cut(s) 93, 145, 656
NlaIII CATG 3 cut(s) 199, 265, 663
NlaIV GGNNCC 2 cut(s) 34, 79
PagI TCATGA 2 cut(s) 261, 659
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 538
PfeI GAWTC 5 cut(s) 7, 122, 258, 462, 636
PkrI GCNGC 3 cut(s) 32, 386, 622
PleI GAGTC 1 cut(s) 110
PluTI GGCGCC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 110
Psp124BI GAGCTC 1 cut(s) 547
Psp6I CCWGG 2 cut(s) 125, 141
PspGI CCWGG 2 cut(s) 125, 141
PspN4I GGNNCC 2 cut(s) 34, 79
PspPI GGNCC 1 cut(s) 574
PstI CTGCAG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 93
RsaI GTAC 2 cut(s) 236, 608
RsaNI GTAC 2 cut(s) 235, 607
SacI GAGCTC 1 cut(s) 547
SalI GTCGAC 1 cut(s) 425
SatI GCNGC 3 cut(s) 31, 385, 621
Sau3AI GATC 3 cut(s) 93, 145, 656
Sau96I GGNCC 1 cut(s) 574
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 127, 143
SduI GDGCHC 1 cut(s) 547
SetI ASST 4 cut(s) 108, 173, 547, 587
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 652
SfoI GGCGCC 1 cut(s) 79
SsiI CCGC 4 cut(s) 30, 66, 110, 385
SspDI GGCGCC 1 cut(s) 77
SstI GAGCTC 1 cut(s) 547
StyD4I CCNGG 2 cut(s) 125, 141
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 5 cut(s) 37, 228, 503, 525, 648
TaiI ACGT 1 cut(s) 587
TaqI TCGA 4 cut(s) 323, 426, 465, 541
TaqII GACCGA 1 cut(s) 474
TatI WGTACW 1 cut(s) 606
TauI GCSGC 2 cut(s) 33, 387
TfiI GAWTC 5 cut(s) 7, 122, 258, 462, 636
TscAI CASTG 2 cut(s) 373, 508
TseI GCWGC 1 cut(s) 620
TspDTI ATGAA 6 cut(s) 239, 250, 278, 323, 527, 569
TspRI CASTG 2 cut(s) 373, 508
XmiI GTMKAC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.