Rorug05G0365600

Pentatricopeptide repeat-containing protein At1g07590

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012552
Physical Location & Seq
Forward (+)
48714093 .. 48714608
516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0365600.1

Sequence Viewer

Length: 516 bp
ATGAGAAGCTTTGGAATGATTTTGCTATTGCCGCTCCCTGCTATTGTCGCTAGTACTATGGCCTGGTTTGAAGAATATAGCCGCATAAACAAGGCTACTGGAAGTCAGGCTTCTCCTCGAATTAAAGGCAATCAACCTTGGCGCCCACCTATAGCAGGGATGCTAAAATTGAACACAGATGGAGCATTTCTACAGTTAGCAACTCATGGAGGGGTGGGAGGTGTCTTGCGGAATGAAAAGGGTGAATTCATTGCTGGATTTGCTTATCATAAAGAGTATGTCACTTCTGCAATGCATGTGGAACTTCTTGCAATTAGACATGGGCTAGAATTGTTGCAAGCCATGACAGTAACGAATGTTATTGTTCAAAGTGATTGTCTCTTGGCTATAAAGGCTATTTTTGATGAGGGTGAGGATTTCACAACTCTAGGAAACTTAGTGGACGAGATATATCAAGGCTCTTCTTCATTAGTTGCCTTCGATTGGGATACAACATGCTTATCGAACCTCCAATAG

Protein Analysis

171

Amino Acids

18.71

Weight (kDa)

5.28

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 58 - 160 3.8e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07590
fragaria_vesca FvH4_3g36060 FvH4_3g36060
malus_domestica MD11G1109000.v1.1
prunus_persica Prupe.6G077700_v2.0.a1
pyrus_communis pycom11g09010
rosa_chinensis RchiOBHm_Chr5g0064501
rosa_laevigata RLG00000035729
rosa_multiflora Rmu_sc0003352.1_g000001 Rmu_sc0018367.1_g000006 Rmu_sc0032871.1_g000002
rosa_roxburghii Rroxscaffold_1G00016520
rosa_rugosa Rorug05G0365500 Rorug05G0365600
rosa_samantha Rh5AG423600 Rh5BG439000 Rh5CG461700 Rh5DG452300
rosa_wichuraiana Rw5G039820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccBSI CCGCTC 1 cut(s) 34
AciI CCGC 3 cut(s) 32, 82, 229
AcsI RAATTY 1 cut(s) 245
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 155, 483
AgsI TTSAA 3 cut(s) 71, 172, 368
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 1 cut(s) 9
AluI AGCT 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 383
AoxI GGCC 1 cut(s) 60
ApoI RAATTY 1 cut(s) 245
ArsI GACNNNNNNTTYG 2 cut(s) 361, 393
AspLEI GCGC 1 cut(s) 144
AsuHPI GGTGA 2 cut(s) 254, 422
BanI GGYRCC 1 cut(s) 141
BccI CCATC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 64
BciVI GTATCC 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 3 cut(s) 51, 326, 428
BfmI CTRYAG 2 cut(s) 150, 191
BfoI RGCGCY 1 cut(s) 145
BfuI GTATCC 1 cut(s) 481
BisI GCNGC 2 cut(s) 32, 82
BlsI GCNGC 2 cut(s) 33, 83
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 64
BmsI GCATC 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 137
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 483
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 2 cut(s) 249, 297
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 155, 483
BseMI GCAATG 2 cut(s) 249, 297
BseNI ACTGG 1 cut(s) 103
BseRI GAGGAG 1 cut(s) 105
BshFI GGCC 1 cut(s) 62
BshNI GGYRCC 1 cut(s) 141
BslI CCNNNNNNNGG 2 cut(s) 155, 483
BsmAI GTCTC 1 cut(s) 383
BsnI GGCC 1 cut(s) 62
BspACI CCGC 3 cut(s) 32, 82, 229
BspANI GGCC 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 143
BspQI GCTCTTC 1 cut(s) 466
BspT107I GGYRCC 1 cut(s) 141
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 2 cut(s) 249, 297
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 137
BssNI GRCGYC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 195, 349
Bst6I CTCTTC 1 cut(s) 466
BstACI GRCGYC 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 1 cut(s) 436
BstENI CCTNNNNNAGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 165
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 1 cut(s) 144
BstMAI GTCTC 1 cut(s) 383
BstMWI GCNNNNNNNGC 4 cut(s) 31, 47, 260, 392
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 2 cut(s) 299, 498
BstSCI CCNGG 1 cut(s) 62
BstSFI CTRYAG 2 cut(s) 150, 191
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 339
CfoI GCGC 1 cut(s) 144
Csp6I GTAC 1 cut(s) 54
CspCI CAANNNNNGTGG 2 cut(s) 279, 314
CviAII CATG 5 cut(s) 206, 296, 320, 343, 495
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 1 cut(s) 436
DinI GGCGCC 1 cut(s) 143
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 137
EcoNI CCTNNNNNAGG 1 cut(s) 153
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 297
EgeI GGCGCC 1 cut(s) 143
EheI GGCGCC 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 5 cut(s) 209, 299, 323, 346, 498
FalI AAGNNNNNCTT 2 cut(s) 94, 126
FatI CATG 5 cut(s) 205, 295, 319, 342, 494
Fnu4HI GCNGC 2 cut(s) 32, 82
FokI GGATG 1 cut(s) 172
Fsp4HI GCNGC 2 cut(s) 32, 82
FspBI CTAG 3 cut(s) 51, 326, 428
GlaI GCGC 1 cut(s) 143
GluI GCNGC 2 cut(s) 32, 82
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 1 cut(s) 62
HhaI GCGC 1 cut(s) 144
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 5 cut(s) 209, 299, 323, 346, 498
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 7
HphI GGTGA 2 cut(s) 254, 422
Hpy166II GTNNAC 1 cut(s) 442
Hpy8I GTNNAC 1 cut(s) 442
HpyAV CCTTC 1 cut(s) 487
HpyCH4III ACNGT 2 cut(s) 195, 349
HpyCH4V TGCA 4 cut(s) 290, 295, 311, 337
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 47, 260, 392
HpyF3I CTNAG 1 cut(s) 436
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 5 cut(s) 209, 299, 323, 346, 498
HspAI GCGC 1 cut(s) 142
KasI GGCGCC 1 cut(s) 141
LguI GCTCTTC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 39, 182
LpnPI CCDG 7 cut(s) 49, 51, 76, 84, 92, 141, 240
LweI GCATC 1 cut(s) 150
MaeI CTAG 3 cut(s) 51, 326, 428
MaeIII GTNAC 2 cut(s) 280, 349
MbiI CCGCTC 1 cut(s) 34
MboII GAAGA 3 cut(s) 83, 453, 456
MluCI AATT 5 cut(s) 120, 167, 245, 312, 329
Mly113I GGCGCC 1 cut(s) 142
MnlI CCTC 5 cut(s) 126, 203, 212, 400, 406
Mph1103I ATGCAT 1 cut(s) 297
MseI TTAA 1 cut(s) 123
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 4 cut(s) 31, 47, 260, 392
NarI GGCGCC 1 cut(s) 142
NlaIII CATG 5 cut(s) 209, 299, 323, 346, 498
NlaIV GGNNCC 1 cut(s) 143
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 297
NspI RCATGY 2 cut(s) 299, 498
PciSI GCTCTTC 1 cut(s) 466
PkrI GCNGC 2 cut(s) 33, 83
PluTI GGCGCC 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 143
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 466
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 2 cut(s) 32, 82
ScaI AGTACT 1 cut(s) 55
ScrFI CCNGG 1 cut(s) 64
SetI ASST 5 cut(s) 11, 139, 151, 223, 510
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 2 cut(s) 150, 191
SfoI GGCGCC 1 cut(s) 143
Sse9I AATT 5 cut(s) 120, 167, 245, 312, 329
SsiI CCGC 3 cut(s) 32, 82, 229
SspDI GGCGCC 1 cut(s) 141
SspMI CTAG 3 cut(s) 51, 326, 428
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 137
TaaI ACNGT 2 cut(s) 195, 349
TaqI TCGA 3 cut(s) 118, 480, 503
TasI AATT 5 cut(s) 120, 167, 245, 312, 329
TatI WGTACW 1 cut(s) 53
TauI GCSGC 2 cut(s) 34, 84
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseFI GTSAC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 3 cut(s) 238, 249, 456
XagI CCTNNNNNAGG 1 cut(s) 153
XapI RAATTY 1 cut(s) 245
XceI RCATGY 2 cut(s) 299, 498
XspI CTAG 3 cut(s) 51, 326, 428
ZrmI AGTACT 1 cut(s) 55
Zsp2I ATGCAT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.