Rorug05G0408000

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012511
Physical Location & Seq
Forward (+)
56262340 .. 56283735
21396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0408000.1

Sequence Viewer

Length: 1632 bp
ATGGAAACATCTGGGTATGGCCGTGATGGAGTTTTCAGGTCACTCAGACCACCTCTGGTACTACCCAGAGACCCAAATCTCAGCATGGTCTCTTCCCTCTTCAAAAACTCTTCTTCTTATCCACACAAACCTGCCCTCATTGATGCCGAGTCCTCTGAAACCTTGTCCTTCTCCCAGCTCAAGTCAACGGTCATCAAGGTTGCCCATGGCTTAATCCATCTGGGTATTAAGAAACACGACGTGGTTCTGATTTTCGCTCCCAATTCAATCCAGTTTCCTATTTGTTTCCTTGGAATTATTGCAGTTGGTGCCATTGCAACAACTTCAAATCCTCTCTACACTGTTTCAGAGCTTTCCAAGCAAGTCAAGGATTCCAATCCAAAACTGGTTATAACTGTGCCTGAATTGTGGGAAAAGGTGAAGGGTTTAAATTTTCCAGCAGTGATTCTAGGACCGAAAGGTTCATCTTCCCAAATTGAATCCAGCTCAAGAATTGTGGGTTTTCATGATTTGGTTCAGTTATCTGGGCCTGTCTCGGATTTCCCTAGTGTAAATGTTAAGCAGACTGATACTGCTGCGCTGTTGTACTCTTCAGGTACAACAGGCCTCAGCAAAGGTGTGATTCTGACTCATGGGAATTTCATTGCTTCCTCTCTGATGATCACACAAGATCAAGAAGTGGCTGGAGAGATGCACCATGTGTTTCTCTGTGTGTTGCCAATGTTCCATATGTTTGGATTGGCCGTCATCACTTACGCGCAGCTGCGAAAGGGAAATGCTGTGATTTCGATGTCCAGATTCAATCTGGAGAAGATTTTGATGACTGTTGAGAAGTACAAGGTCACCCATTTGTGGGTTGTGCCTCCTATTATACTTGCTCTGTCAAAGGACAGTGTGGTTAAGAAGTACAATCTTTCATCTTTGAAGCATATTGCGTCTGGTGCAGCTCCTCTGGCGAAAGAGTTGATGGAGGAGTGTGCAAAAATTATTCCTCAAGGTGTAGTTATTCAGGGTTATGGTATGACAGAAACCTGTGGAATTATTTCAATTGAGAATTCACTAGTAGGACCTCGACACAGTGGTTCAGCTGGCACTCTTGTTTCTGGAGTTGAATCTCAGATAGTCAGTGTAGATACACTGAAGCCTCTCCCTCCGAAGCAGTTGGGGGAGATATGGGTTAGGGGGCCTAGTATGATGATGGGTTATTTTAACAACCCACAGGCCACCCAGCTAACTTTAGATAAAAATGGTTGGGTACATACGGGAGATCTTGGATACTTTGATGAAAGTGGCCAACTTTTTGTGGTTGACCGAATTAAAGAACTCATTAAGTATAAAGGTTTTCAGGTAGCACCAGCTGAACTTGAAGGCCTGTTAGTTTCTCACCCTGAAATATTAGACGCCGTTGTCATCCCATTTCCTGATGCTGAAGCTGGTGAGGTCCCTGTTGCATATGTTGTGCGCTCGCCAAACAGTTCACTGACCGAAGAAGATATCAAGAGTTTTATAGCGAGTCAGGTTGCATCTTTCAAAAGACTGCGACGAGTGACATTCATAAACACTGTCCCCAAGTCGGCATCAGGAAAAATTCTCAGAAAAGAGCTCATCGAGAAAGTACGGTCCAAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 ubiquitin

Protein Analysis

543

Amino Acids

59.01

Weight (kDa)

8.86

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 35 - 403 1.4e-86 AMP-binding enzyme
AMP-binding_C PF13193 454 - 529 9.9e-17 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30100
fragaria_vesca FvH4_3g39290 FvH4_3g39290 FvH4_3g39290
malus_domestica MD03G1062600.v1.1 MD11G1066200.v1.1
prunus_persica Prupe.6G052600_v2.0.a1
pyrus_communis pycom03g05020
rosa_chinensis RchiOBHm_Chr5g0070721
rosa_laevigata RLG00000036153
rosa_multiflora Rmu_sc0002944.1_g000042 Rmu_sc0036080.1_g000003
rosa_roxburghii Rroxscaffold_1G00010470
rosa_rugosa Rorug05G0408000
rosa_samantha Rh5AG463500 Rh5BG481300 Rh5CG505600 Rh5DG493900
rosa_wichuraiana Rw5G043140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AasI GACNNNNNNGTC 1 cut(s) 1406
Acc36I ACCTGC 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 1 cut(s) 758
AcoI YGGCCR 3 cut(s) 19, 741, 1291
AcsI RAATTY 4 cut(s) 430, 637, 1054, 1587
AcuI CTGAAG 3 cut(s) 576, 1160, 1449
AcyI GRCGYC 1 cut(s) 1401
AdeI CACNNNGTG 2 cut(s) 241, 700
AfaI GTAC 7 cut(s) 60, 587, 598, 836, 908, 1257, 1617
AfiI CCNNNNNNNGG 3 cut(s) 852, 853, 1573
AhlI ACTAGT 1 cut(s) 1060
AjiI CACGTC 1 cut(s) 241
Alw21I GWGCWC 1 cut(s) 1605
Alw26I GTCTC 3 cut(s) 63, 94, 538
AoxI GGCC 8 cut(s) 19, 527, 604, 741, 1184, 1221, 1291, 1369
ApeKI GCWGC 4 cut(s) 575, 760, 763, 944
ApoI RAATTY 4 cut(s) 430, 637, 1054, 1587
Asp700I GAANNNNTTC 1 cut(s) 1042
AspLEI GCGC 3 cut(s) 580, 760, 1464
AspS9I GGNCC 6 cut(s) 452, 527, 1067, 1184, 1441, 1620
AsuHPI GGTGA 4 cut(s) 430, 835, 1376, 1448
AvaII GGWCC 4 cut(s) 452, 1067, 1441, 1620
BalI TGGCCA 1 cut(s) 1293
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 1605
Bbv12I GWGCWC 1 cut(s) 1605
BbvCI CCTCAGC 1 cut(s) 608
BbvI GCAGC 4 cut(s) 562, 750, 772, 956
BccI CCATC 4 cut(s) 20, 225, 961, 1192
BceAI ACGGC 3 cut(s) 6, 728, 1388
BciVI GTATCC 1 cut(s) 1268
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 3 cut(s) 63, 94, 538
BcuI ACTAGT 1 cut(s) 1060
BfaI CTAG 4 cut(s) 449, 546, 1061, 1188
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 1 cut(s) 1268
BglII AGATCT 1 cut(s) 1267
BisI GCNGC 4 cut(s) 576, 761, 764, 945
BlsI GCNGC 4 cut(s) 577, 762, 765, 946
Bme18I GGWCC 4 cut(s) 452, 1067, 1441, 1620
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 6 cut(s) 452, 527, 1067, 1184, 1441, 1620
BmiI GGNNCC 3 cut(s) 310, 1185, 1443
BmsI GCATC 5 cut(s) 133, 681, 1414, 1532, 1586
BpmI CTGGAG 3 cut(s) 705, 827, 1125
Bpu10I CCTNAGC 1 cut(s) 608
BpuEI CTTGAG 3 cut(s) 164, 472, 978
BsaBI GATNNNNATC 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 1401
BsaI GGTCTC 2 cut(s) 63, 94
BsaJI CCNNGG 2 cut(s) 205, 289
Bsc4I CCNNNNNNNGG 3 cut(s) 852, 853, 1573
Bse1I ACTGG 2 cut(s) 271, 390
Bse3DI GCAATG 2 cut(s) 312, 642
Bse8I GATNNNNATC 1 cut(s) 375
BseDI CCNNGG 2 cut(s) 205, 289
BseGI GGATG 1 cut(s) 1410
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 3 cut(s) 852, 853, 1573
BseMI GCAATG 2 cut(s) 312, 642
BseMII CTCAG 5 cut(s) 58, 94, 622, 1130, 1606
BseNI ACTGG 2 cut(s) 271, 390
BseRI GAGGAG 2 cut(s) 939, 986
BseXI GCAGC 4 cut(s) 562, 750, 772, 956
BseYI CCCAGC 2 cut(s) 174, 1227
BsgI GTGCAG 1 cut(s) 963
Bsh1236I CGCG 1 cut(s) 758
BshFI GGCC 8 cut(s) 21, 529, 606, 743, 1186, 1223, 1293, 1371
BshNI GGYRCC 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 1605
BslFI GGGAC 2 cut(s) 1427, 1550
BslI CCNNNNNNNGG 3 cut(s) 852, 853, 1573
BsmAI GTCTC 3 cut(s) 63, 94, 538
BsmFI GGGAC 2 cut(s) 1427, 1550
BsnI GGCC 8 cut(s) 21, 529, 606, 743, 1186, 1223, 1293, 1371
Bso31I GGTCTC 2 cut(s) 63, 94
Bsp1286I GDGCHC 1 cut(s) 1605
Bsp143I GATC 3 cut(s) 660, 670, 1267
Bsp19I CCATGG 1 cut(s) 205
BspANI GGCC 8 cut(s) 21, 529, 606, 743, 1186, 1223, 1293, 1371
BspCNI CTCAG 5 cut(s) 57, 93, 621, 1129, 1605
BspFNI CGCG 1 cut(s) 758
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 3 cut(s) 310, 1185, 1443
BspMI ACCTGC 1 cut(s) 139
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 2 cut(s) 63, 94
BsrDI GCAATG 2 cut(s) 312, 642
BsrI ACTGG 2 cut(s) 271, 390
BssECI CCNNGG 2 cut(s) 205, 289
BssMI GATC 3 cut(s) 660, 670, 1267
BssNI GRCGYC 1 cut(s) 1401
BssT1I CCWWGG 2 cut(s) 205, 289
Bst4CI ACNGT 9 cut(s) 190, 343, 397, 826, 893, 1079, 1475, 1564, 1620
Bst6I CTCTTC 4 cut(s) 97, 104, 115, 595
BstACI GRCGYC 1 cut(s) 1401
BstAPI GCANNNNNTGC 1 cut(s) 308
BstC8I GCNNGC 2 cut(s) 1090, 1466
BstDEI CTNAG 5 cut(s) 44, 80, 608, 1116, 1592
BstDSI CCRYGG 1 cut(s) 205
BstEII GGTNACC 1 cut(s) 841
BstF5I GGATG 1 cut(s) 1410
BstFNI CGCG 1 cut(s) 758
BstHHI GCGC 3 cut(s) 580, 760, 1464
BstKTI GATC 3 cut(s) 663, 673, 1270
BstMAI GTCTC 3 cut(s) 63, 94, 538
BstMBI GATC 3 cut(s) 660, 670, 1267
BstMWI GCNNNNNNNGC 4 cut(s) 308, 358, 941, 953
BstPI GGTNACC 1 cut(s) 841
BstUI CGCG 1 cut(s) 758
BstV1I GCAGC 4 cut(s) 562, 750, 772, 956
BstX2I RGATCY 1 cut(s) 1267
BstXI CCANNNNNNTGG 1 cut(s) 734
BstYI RGATCY 1 cut(s) 1267
BsuI GTATCC 1 cut(s) 1268
BsuRI GGCC 8 cut(s) 21, 529, 606, 743, 1186, 1223, 1293, 1371
BtgI CCRYGG 1 cut(s) 205
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 1 cut(s) 1410
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 8 cut(s) 339, 447, 898, 1084, 1132, 1136, 1478, 1560
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 1090, 1466
CciI TCATGA 1 cut(s) 505
CfoI GCGC 3 cut(s) 580, 760, 1464
Cfr13I GGNCC 6 cut(s) 452, 527, 1067, 1184, 1441, 1620
CseI GACGC 2 cut(s) 924, 1409
Csp6I GTAC 7 cut(s) 59, 586, 597, 835, 907, 1256, 1616
CspCI CAANNNNNGTGG 2 cut(s) 477, 512
CviAII CATG 5 cut(s) 85, 206, 506, 632, 698
CviQI GTAC 7 cut(s) 59, 586, 597, 835, 907, 1256, 1616
DdeI CTNAG 5 cut(s) 44, 80, 608, 1116, 1592
DpnI GATC 3 cut(s) 662, 672, 1269
DpnII GATC 3 cut(s) 660, 670, 1267
DraI TTTAAA 1 cut(s) 429
DraIII CACNNNGTG 2 cut(s) 241, 700
DrdI GACNNNNNNGTC 1 cut(s) 1406
DseDI GACNNNNNNGTC 1 cut(s) 1406
EaeI YGGCCR 3 cut(s) 19, 741, 1291
Eam1104I CTCTTC 4 cut(s) 97, 104, 115, 595
EarI CTCTTC 4 cut(s) 97, 104, 115, 595
Ecl136II GAGCTC 1 cut(s) 1603
Eco130I CCWWGG 2 cut(s) 205, 289
Eco147I AGGCCT 2 cut(s) 606, 1371
Eco24I GRGCYC 1 cut(s) 1605
Eco31I GGTCTC 2 cut(s) 63, 94
Eco32I GATATC 1 cut(s) 1495
Eco47I GGWCC 4 cut(s) 452, 1067, 1441, 1620
Eco53kI GAGCTC 1 cut(s) 1603
Eco57I CTGAAG 3 cut(s) 576, 1160, 1449
Eco91I GGTNACC 1 cut(s) 841
EcoICRI GAGCTC 1 cut(s) 1603
EcoO109I RGGNCCY 3 cut(s) 1067, 1184, 1441
EcoO65I GGTNACC 1 cut(s) 841
EcoRI GAATTC 1 cut(s) 1054
EcoRV GATATC 1 cut(s) 1495
EcoT14I CCWWGG 2 cut(s) 205, 289
EcoT38I GRGCYC 1 cut(s) 1605
ErhI CCWWGG 2 cut(s) 205, 289
FaeI CATG 5 cut(s) 88, 209, 509, 635, 701
FaqI GGGAC 2 cut(s) 1427, 1550
FatI CATG 5 cut(s) 84, 205, 505, 631, 697
FauNDI CATATG 2 cut(s) 729, 1453
FbaI TGATCA 1 cut(s) 660
Fnu4HI GCNGC 4 cut(s) 576, 761, 764, 945
FokI GGATG 1 cut(s) 1397
FriOI GRGCYC 1 cut(s) 1605
Fsp4HI GCNGC 4 cut(s) 576, 761, 764, 945
FspBI CTAG 4 cut(s) 449, 546, 1061, 1188
GlaI GCGC 3 cut(s) 579, 759, 1463
GluI GCNGC 4 cut(s) 576, 761, 764, 945
GsaI CCCAGC 2 cut(s) 178, 1231
GsuI CTGGAG 3 cut(s) 705, 827, 1125
HaeIII GGCC 8 cut(s) 21, 529, 606, 743, 1186, 1223, 1293, 1371
HgaI GACGC 2 cut(s) 924, 1409
HhaI GCGC 3 cut(s) 580, 760, 1464
Hin1I GRCGYC 1 cut(s) 1401
Hin1II CATG 5 cut(s) 88, 209, 509, 635, 701
Hin6I GCGC 3 cut(s) 578, 758, 1462
HinP1I GCGC 3 cut(s) 578, 758, 1462
HincII GTYRAC 2 cut(s) 186, 1309
HindII GTYRAC 2 cut(s) 186, 1309
HinfI GANTC 9 cut(s) 149, 371, 445, 479, 622, 628, 798, 1112, 1513
HphI GGTGA 4 cut(s) 430, 835, 1376, 1448
Hpy166II GTNNAC 3 cut(s) 186, 1309, 1478
Hpy8I GTNNAC 3 cut(s) 186, 1309, 1478
Hpy99I CGWCG 2 cut(s) 242, 1545
HpyAV CCTTC 3 cut(s) 178, 415, 1361
HpyCH4III ACNGT 9 cut(s) 190, 343, 397, 826, 893, 1079, 1475, 1564, 1620
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 7 cut(s) 302, 317, 694, 944, 980, 1451, 1523
HpyF10VI GCNNNNNNNGC 4 cut(s) 308, 358, 941, 953
HpyF3I CTNAG 5 cut(s) 44, 80, 608, 1116, 1592
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 1401
Hsp92II CATG 5 cut(s) 88, 209, 509, 635, 701
HspAI GCGC 3 cut(s) 578, 758, 1462
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 3 cut(s) 660, 670, 1267
LmnI GCTCC 2 cut(s) 262, 952
Lsp1109I GCAGC 4 cut(s) 562, 750, 772, 956
LweI GCATC 5 cut(s) 133, 681, 1414, 1532, 1586
MaeI CTAG 4 cut(s) 449, 546, 1061, 1188
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 3 cut(s) 39, 841, 1546
MalI GATC 3 cut(s) 662, 672, 1269
MboI GATC 3 cut(s) 660, 670, 1267
MboII GAAGA 9 cut(s) 84, 91, 102, 105, 459, 582, 823, 1499, 1502
MfeI CAATTG 1 cut(s) 1047
MflI RGATCY 1 cut(s) 1267
MhlI GDGCHC 1 cut(s) 1605
MlsI TGGCCA 1 cut(s) 1293
MluNI TGGCCA 1 cut(s) 1293
MlyI GAGTC 3 cut(s) 158, 622, 1522
Mox20I TGGCCA 1 cut(s) 1293
MroXI GAANNNNTTC 1 cut(s) 1042
MscI TGGCCA 1 cut(s) 1293
MseI TTAA 8 cut(s) 212, 228, 428, 558, 900, 1209, 1317, 1329
Msp20I TGGCCA 1 cut(s) 1293
MspA1I CMGCKG 3 cut(s) 763, 1088, 1358
MunI CAATTG 1 cut(s) 1047
MvnI CGCG 1 cut(s) 758
MwoI GCNNNNNNNGC 4 cut(s) 308, 358, 941, 953
NcoI CCATGG 1 cut(s) 205
NdeI CATATG 2 cut(s) 729, 1453
NdeII GATC 3 cut(s) 660, 670, 1267
NlaIII CATG 5 cut(s) 88, 209, 509, 635, 701
NlaIV GGNNCC 3 cut(s) 310, 1185, 1443
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 3 cut(s) 39, 841, 1546
PagI TCATGA 1 cut(s) 505
PceI AGGCCT 2 cut(s) 606, 1371
PdmI GAANNNNTTC 1 cut(s) 1042
PfeI GAWTC 6 cut(s) 371, 445, 479, 622, 798, 1112
PkrI GCNGC 4 cut(s) 577, 762, 765, 946
PleI GAGTC 3 cut(s) 157, 622, 1521
PpsI GAGTC 3 cut(s) 157, 622, 1521
PpuMI RGGWCCY 2 cut(s) 1067, 1441
PsiI TTATAA 1 cut(s) 392
Psp124BI GAGCTC 1 cut(s) 1605
Psp5II RGGWCCY 2 cut(s) 1067, 1441
PspEI GGTNACC 1 cut(s) 841
PspFI CCCAGC 2 cut(s) 174, 1227
PspN4I GGNNCC 3 cut(s) 310, 1185, 1443
PspPI GGNCC 6 cut(s) 452, 527, 1067, 1184, 1441, 1620
PspPPI RGGWCCY 2 cut(s) 1067, 1441
PsuI RGATCY 1 cut(s) 1267
PvuII CAGCTG 3 cut(s) 763, 1088, 1358
RsaI GTAC 7 cut(s) 60, 587, 598, 836, 908, 1257, 1617
RsaNI GTAC 7 cut(s) 59, 586, 597, 835, 907, 1256, 1616
SacI GAGCTC 1 cut(s) 1605
SaqAI TTAA 8 cut(s) 212, 228, 428, 558, 900, 1209, 1317, 1329
SatI GCNGC 4 cut(s) 576, 761, 764, 945
Sau3AI GATC 3 cut(s) 660, 670, 1267
Sau96I GGNCC 6 cut(s) 452, 527, 1067, 1184, 1441, 1620
SchI GAGTC 3 cut(s) 158, 622, 1522
SduI GDGCHC 1 cut(s) 1605
SfaNI GCATC 5 cut(s) 133, 681, 1414, 1532, 1586
SinI GGWCC 4 cut(s) 452, 1067, 1441, 1620
SmlI CTYRAG 3 cut(s) 179, 487, 993
SmoI CTYRAG 3 cut(s) 179, 487, 993
SpeI ACTAGT 1 cut(s) 1060
SseBI AGGCCT 2 cut(s) 606, 1371
SspI AATATT 1 cut(s) 1395
SspMI CTAG 4 cut(s) 449, 546, 1061, 1188
SstI GAGCTC 1 cut(s) 1605
StuI AGGCCT 2 cut(s) 606, 1371
StyI CCWWGG 2 cut(s) 205, 289
TaaI ACNGT 9 cut(s) 190, 343, 397, 826, 893, 1079, 1475, 1564, 1620
TaiI ACGT 1 cut(s) 243
TaqI TCGA 3 cut(s) 788, 1072, 1608
TaqII GACCGA 3 cut(s) 469, 1326, 1499
TatI WGTACW 3 cut(s) 585, 834, 906
TfiI GAWTC 6 cut(s) 371, 445, 479, 622, 798, 1112
Tru1I TTAA 8 cut(s) 212, 228, 428, 558, 900, 1209, 1317, 1329
Tru9I TTAA 8 cut(s) 212, 228, 428, 558, 900, 1209, 1317, 1329
TscAI CASTG 8 cut(s) 346, 447, 898, 1084, 1132, 1143, 1485, 1567
TseFI GTSAC 3 cut(s) 39, 841, 1546
TseI GCWGC 4 cut(s) 575, 760, 763, 944
Tsp45I GTSAC 3 cut(s) 39, 841, 1546
TspDTI ATGAA 6 cut(s) 453, 494, 631, 906, 1299, 1543
TspRI CASTG 8 cut(s) 346, 447, 898, 1084, 1132, 1143, 1485, 1567
VpaK11BI GGWCC 4 cut(s) 452, 1067, 1441, 1620
XapI RAATTY 4 cut(s) 430, 637, 1054, 1587
XcmI CCANNNNNNNNNTGG 2 cut(s) 382, 802
XmnI GAANNNNTTC 1 cut(s) 1042
XspI CTAG 4 cut(s) 449, 546, 1061, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.