Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0013396

Physical Location & Seq

Genomic Coordinates Forward (+)

59406301 .. 59411286

Gene Structure 4986 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0427300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4305 bp

ATGGGGACTGAATTACCCTCCTCCTCATTTGCCTCTTCTTCAAACACACAATGGACATATGACGTTTTTCTCAGTTTCCGAGGTGAGGATACTCGTAACAGCTTTACAGATCATCTATATGCTGCTCTAGACCGAGAAGGTATTTTCACCTTTAGGGATGATGAAGAGCTTGATAGAGGACAACCCATTTCATCAAATCTCCTGGAAGCCATACAAGTTTCAAGAATCGCGCTTGTTGTTATCTCGAGAAACTATGCTTCTTCGACTTGGTGTTTGGATGAACTCACTATGATTGTTGAGTGCATGGAGGACAGGGGGCAGAGAGTGTTTCCAATTTTCTATGATGTAGATCCATCCGAGGTAAGGAAACAGACCGGATGGTTTGGACAAGCCTTTGTTGTACATGAAGAGCGCTTCAAGGATGACTTGGAGAAAGACAAGGTGCAAAAGTGGAGAGCTGCTTTGACTCGAGTGGCCAATCTCTCAGGATTTCGTTTACGCGATAGGTATGAGTCAAAGTTTATCCAAGAGATTATGGGAAAGATAGTAAATGAGTTGAGCGGTACAATCCAACAGCCTCATTCGGATGATCTTGGACTTGTGGGAATTGACTTTGGTGTTGAGGAAATAATGAAGTTATGTTCAGCTACAGATCAAGGGAATGATGTTGTCTTTGTAGGTATATGGGGAATGGGTGGGGTTGGCAAGACAACTCTTGCACAAGCTTTTTATGATAAAATCTCTGATCAATTTCGAGATAAATGCTTTCTGAAAAATGTTAGAGAGATTTCTAGACGAGACGGTATTGTTGCATTAACAAAAGATTTCCTTTGCAATGTCTTAAAAAGGATGGATGTGCATGACAGTGAGATAAGGCATAGATTTCGTCGTAAAAAGGTTCTCATCATTCTTGATGATGTAGATAAGTTAGAACAATTGCAAGCATTAGCTGGAAGCAACAATTGGTTTGGTCGAGGGAGTAGGATCATTATAACAACCAGGGACGAACAATTGTTGATAGCTCATCATGTGGATAGAAGTTACAAGGTCAAGGAATTGAAAAGTGATGGTGCACTGGAGCTATTTAGTTGGAAAGCCTTCAGGAGTGCGCATCCCCCAGAAGATTATTTGGAGTTATCCCATAACTTTGTAAATTACTGTAAGGGCCTTCCTTTAGCTATCAAAGTTTTGGGTGCTTTCTTGTGTGGTCAAAGTCCTCAAGAATGGAAAAGTGCCTTTAGTAGGATACAAGAAAATCTCGACTATGATATTATGTCTGCCCTTAGAATAAGCTTCGATGGACTGCATGAGCAGGAGAAGCAAATATTTTTGGATATTGCATGTTTCTTCAAAGGGCAGGACAGAGATCAAGTGGCTGTTATATTAGAGAGTTTTGGTTTTCACCCGATCATTGGTATAAGAGCTCTCATCAGAAAATCTCTGATCACTTTAGTGGGGAATAAATTGTGGATGCATGATTTGCTTCAGGAAATGGGTTGGGAACTTGTTCGTGCGGAATGCCTCAATGAGCCTGGGAACTGTAGTAGAATCTGGCAATCCGCTGATGTGCTTGATGTAATGAAGAATAAAAAGGGATCAGATGCGGTTCAAGGGATGGTCCTTGTTTTGCCTCAAGAACGAAAGGAAGAATTGAAAAGTGACGCCTTGTCAAACTTGAAAAAGTTGAGATTTCTCAAAATATCTAATGTGCTCCTTCCTCTTGGCCTCGATTATCTATCTACTGAGTTAAGAGCTCTCGAATGGCATGGATATCCTTCAAAGTTGTTGCCAAGAGCTTTTCCATCTGAGAAGCTTGTTCACCTCAACCTGTGTGGCAGCCATATCAAACATCTATGGAAAGGATTCAAGTGTTTAGATAAGTTAAAATCAATTGATTTGAAGGACTCTGAAAGTTTGATTGAGACACCAGACTTCAGGGGTGTCCCAAATCTTGAGAGGCTGATTCTTGAAGGTTGCACAAAATTATCTAAGGTTCACCCGTCCATTTGCAATCTCAAACACCTTAGATTATTGAATATGAAAAACTGTGAAAGCCTTCGGATCACTTCATGGGACATCAGTTTGGAATCTCTCGAAATTTTCAATCTTTCGGGCTGTGCACGACTCAAGAGATTTCCAGAGATTGTGGGAGATATGAAATGCTTGTCTAAAATTTATTTAGATGGGAGTGCTATAGAGGAATTACCAGTGTCAATTGAGCGTCTGACTGGTCTTACATTGTTGGATATGGCTCATTGCAAAAATATTTTGAGAATTCCTAGTGTCATTTCTAGTTTGACGAGACTTAAAACAGTCATTCTATCAGGCTGCTCTAATCTTATTGAAATTCCCGAGAGTATTGGGAATGTGGAATGTCTTGAAGAGTTGGATATAAGCGGAACTGCTATAAGGCAAATGCCATCATCCGTTGTGTATCTCAAAAACCTCAAACAGTTATCCTGCTCCGGGTGCAGTGGTCAAGTTTTATGCCCTGGACCGAACTTGTTGTTACCTGCTTCATCGTCTGGTTTTAGCTCTTTAATGTCATTGAATTTAAGTTACTGCAATCTGTTTGATGGAGCTCTCCCTAATTATTTGGACCGCATGTCATCATTGAAGATGTTGGATTTACAGGGAAACAATTTTACAAGCATACCTGAAAGTTTTTGCCGACTTTCTAAGCTTGAAATACTTCACTTGGATAATTGCAGCCGATTACGGTCATTGCCAAAGCTTCCATCGAATATACAAATGGTTGGGGCAACTGGTTGTATGTCACTTAAAAGTGATTCTGATCAGTATGGAGTATTTGGTTCAGCATATGAAAACCATGTTTGGATTACTAGGTGCACAACATCGACCCTATATTACATTGTCATGTTCGATTCACAATTTGCAGCATGGCTGAACAGATATCATGAGGTACAGGTTGAGATAAGTTTACCGGTGAGAACTGCATATGGTTTATCAATTAGAGTTCATGGAAATATTCCAAATTGGTTCAACAATAGAAGCAGCAACTTTTCTATAACAATCCGGCTTTCAAAGGACTTAACTAATAAGAGTAGGCTATGGATGGGATGTGCTTTCTATGTCGGTTTGGAAGTTGATGAGAATTTTGATACCGGTTCTGATTTCAAAGTCAGGCACAAGTTTGACTGTTTGTTTGATACCATGGAAGGTCCTGGCTACAAACAAACTCTATACCACGAGGTAAATGCTAAAGGCGTCCATTTCACTGGGTCAATTCACTCAATTGGATATTGGATAATAATACCGCATCTGTGGTTTTCAGCAAGGTTGAGTGCCTTCAATCAGTGCAGTAGCATCGAGGCTAACACTACAATTGATAGCCCATACGTGGAGGTTAAAATGAGTGGTGCCCGTCTACTCTACGAAATAACAGATATTATAGAGTTTATCCAAGCAATAAAAAGCTCGACTTGGCCTTCAAGAATTAGGCAGAATAACAGTTCCTCTGTAAGTCTGTTGGCTGAGGATGGTAGCTGCAAAATTGACTCGAGTATTTCGTTGAAAGAAAATCTTAGCTCGCTGCCTTCAGATTTAAAATGCTCTGAGGGGCAATGCGAGGAGCGATTCAAGCACTACGTTATTGAGGGCAAAGTCGTTCATCCCCAGACCTCGTCTCTGTTAGGAGAAATCCTTCAGATATATTCTGGTCAGCACTGCGTGTATTATAGAGAAACTTTTCCTCAATATAGAATTCCAGAATGGTTCTGCAGTGTGAATGGTTCCTCCGCAGCCTTCCCGCTAAATCCTATGAATGGGTGGAAAGGAATAGCTGCATGTGCCGTTTTCACAGTCCTTGACAGTTTGGATCCTGAAAAGACTCACATACACTGTGATTTAGGAACTGACTATCGGTGTATCACAATAGAGGGACGCATCACTGAATCTTTTATTAGTTTGAAGGTTCGTGGTTTCGTGGGTATGTTTTATGCATCACGTGGGTCACTTTCACATCTTATGTCATCTGATTGCACTTCCACCATCAAAGCCGTGTTTTATAGTAACAGCAAGAACATAACGGTGCAGAGTTGTGGGTATCAAATTGTATACCATGAAAATATGGAAGAATTTATGGAAACAATACTGCAATGTTCAATCGCATCTCAGACTTCTCAGTTGTCAAAAGATACAAGTAGAGAAGAAACACCGAAAGAAAAGGGGAACTCTCTCTACCCAACGGCTGTGTTTGGTCCCAGAATCAGAGCCTTGGGAAAGGAAATTCATTCCTTTCCAGTGTCTTTCCAGTGTTTGGGATTGCAAAGGAAGGGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009941 GO:0009987 GO:0010257 GO:0010258 GO:0010598 GO:0016020 GO:0016043 GO:0022607 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0034357 GO:0034622 GO:0042651 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055035 GO:0065003 GO:0071840 GO:0098542 GO:0098796

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NdhN

Protein Analysis

1434

Amino Acids

162.13

Weight (kDa)

6.56

Isoelectric Point (pI)

43.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	20 - 196	9.6e-55	TIR domain
TIR_2	PF13676	22 - 118	2.8e-15	TIR domain
NB-ARC	PF00931	218 - 369	4.6e-18	NB-ARC domain
NACHT	PF05729	228 - 362	1.2e-08	NACHT domain
WHD_ROQ1	PF23282	437 - 505	1.4e-16	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	664 - 771	1e-07	Leucine-rich repeat region
LRR_13	PF23286	695 - 782	2.6e-12	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	763 - 823	3.4e-08	Leucine-rich repeat region
LRR_13	PF23286	781 - 932	1.5e-06	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8	PF13855	846 - 903	4.5e-08	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013396)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58260 AT5G58260
fragaria_vesca	FvH4_3g41140
malus_domestica	MD03G1047200.v1.1 MD11G1049700.v1.1
prunus_persica	Prupe.6G038400_v2.0.a1
pyrus_communis	pycom03g03720 pycom11g04030
rosa_chinensis	RchiOBHm_Chr5g0073911
rosa_laevigata	RLG00000036424
rosa_multiflora	Rmu_sc0000470.1_g000017
rosa_roxburghii	Rroxscaffold_1G00007460
rosa_rugosa	Rorug05G0427300
rosa_samantha	Rh5AG484300 Rh5BG505000 Rh5CG528800 Rh5DG517300
rosa_wichuraiana	Rw5G045020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	992
Acc16I	TGCGCA	1 cut(s)	1110
Acc36I	ACCTGC	1 cut(s)	2521
AccB1I	GGYRCC	1 cut(s)	3380
AccB7I	CCANNNNNTGG	1 cut(s)	4279
AccBSI	CCGCTC	1 cut(s)	561
AccI	GTMKAC	2 cut(s)	3388, 4077
AccII	CGCG	2 cut(s)	230, 501
AciI	CCGC	9 cut(s)	561, 1514, 1560, 1604, 2397, 2602, 3278, 3759, 3770
AclWI	GGATC	6 cut(s)	344, 992, 1603, 2069, 3833, 3846
AcoI	YGGCCR	1 cut(s)	474
AcsI	RAATTY	9 cut(s)	2097, 2172, 2274, 2346, 2551, 3115, 3723, 4097, 4248
AcuI	CTGAAG	5 cut(s)	1084, 1469, 1918, 3543, 3650
AcvI	CACGTG	1 cut(s)	3968
AcyI	GRCGYC	2 cut(s)	1662, 3228
AdeI	CACNNNGTG	1 cut(s)	3691
AfaI	GTAC	3 cut(s)	402, 565, 2925
AfeI	AGCGCT	1 cut(s)	413
AfiI	CCNNNNNNNGG	8 cut(s)	85, 363, 1242, 1412, 2466, 3361, 3785, 4279
AgeI	ACCGGT	2 cut(s)	2944, 3125
AhdI	GACNNNNNGTC	2 cut(s)	1666, 2605
AjnI	CCWGG	5 cut(s)	201, 998, 1531, 2491, 3184
AjuI	GAANNNNNNNTTGG	2 cut(s)	946, 978
AleI	CACNNNNGTG	1 cut(s)	1451
Alw21I	GWGCWC	7 cut(s)	1075, 1426, 1713, 1756, 2122, 2584, 2852
Alw26I	GTCTC	4 cut(s)	792, 1916, 2296, 3651
Alw44I	GTGCAC	3 cut(s)	1071, 2118, 2848
AlwI	GGATC	6 cut(s)	344, 992, 1603, 2069, 3833, 3846
Ama87I	CYCGRG	4 cut(s)	244, 468, 2351, 3520
Aor51HI	AGCGCT	1 cut(s)	413
AoxI	GGCC	4 cut(s)	474, 1165, 1723, 3446
ApaLI	GTGCAC	3 cut(s)	1071, 2118, 2848
ApoI	RAATTY	9 cut(s)	2097, 2172, 2274, 2346, 2551, 3115, 3723, 4097, 4248
ArsI	GACNNNNNNTTYG	4 cut(s)	256, 288, 3149, 3181
AsiGI	ACCGGT	2 cut(s)	2944, 3125
Asp700I	GAANNNNTTC	7 cut(s)	1097, 1506, 1862, 2055, 2691, 3663, 3708
AspLEI	GCGC	3 cut(s)	232, 414, 1111
AspS9I	GGNCC	6 cut(s)	1165, 1618, 2495, 2599, 3182, 4220
AsuC2I	CCSGG	1 cut(s)	2467
AsuHPI	GGTGA	6 cut(s)	95, 139, 1394, 1811, 1988, 2959
AvaI	CYCGRG	4 cut(s)	244, 468, 2351, 3520
AvaII	GGWCC	5 cut(s)	1618, 2495, 2599, 3182, 4220
BaeGI	GKGCMC	4 cut(s)	1075, 2122, 2852, 3385
BalI	TGGCCA	1 cut(s)	476
BamHI	GGATCC	1 cut(s)	3838
BanI	GGYRCC	1 cut(s)	3380
BanII	GRGCYC	3 cut(s)	1426, 1756, 2584
BauI	CACGAG	1 cut(s)	3209
BbrPI	CACGTG	1 cut(s)	3968
Bbv12I	GWGCWC	7 cut(s)	1075, 1426, 1713, 1756, 2122, 2584, 2852
BbvCI	CCTCAGC	1 cut(s)	3495
BceAI	ACGGC	3 cut(s)	3797, 4004, 4224
BcgI	CGANNNNNNTGC	8 cut(s)	744, 778, 866, 900, 3200, 3234, 3310, 3344
BciT130I	CCWGG	5 cut(s)	203, 1000, 1533, 2493, 3186
BciVI	GTATCC	2 cut(s)	82, 1239
BclI	TGATCA	3 cut(s)	745, 1443, 2794
BcnI	CCSGG	1 cut(s)	2467
BcoDI	GTCTC	4 cut(s)	792, 1916, 2296, 3651
BfaI	CTAG	5 cut(s)	128, 792, 2280, 2292, 2844
BfmI	CTRYAG	4 cut(s)	648, 1540, 2193, 3739
BfoI	RGCGCY	1 cut(s)	415
BfuAI	ACCTGC	1 cut(s)	2521
BfuI	GTATCC	2 cut(s)	82, 1239
Bme1390I	CCNGG	6 cut(s)	203, 1000, 1533, 2467, 2493, 3186
Bme18I	GGWCC	5 cut(s)	1618, 2495, 2599, 3182, 4220
BmeRI	GACNNNNNGTC	2 cut(s)	1666, 2605
BmeT110I	CYCGRG	4 cut(s)	244, 468, 2351, 3520
BmgT120I	GGNCC	6 cut(s)	1165, 1618, 2495, 2599, 3182, 4220
BmiI	GGNNCC	4 cut(s)	3382, 3754, 3840, 4222
BmrFI	CCNGG	6 cut(s)	203, 1000, 1533, 2467, 2493, 3186
BmrI	ACTGGG	1 cut(s)	3249
BmsI	GCATC	8 cut(s)	1120, 1461, 1591, 3289, 3336, 3915, 3971, 4139
BmuI	ACTGGG	1 cut(s)	3249
BpmI	CTGGAG	1 cut(s)	1097
Bpu10I	CCTNAGC	1 cut(s)	3495
BpuEI	CTTGAG	4 cut(s)	1203, 1617, 1973, 2111
BpuMI	CCSGG	1 cut(s)	2467
BsaAI	YACGTR	2 cut(s)	3361, 3968
BsaBI	GATNNNNATC	2 cut(s)	348, 2793
BsaHI	GRCGYC	2 cut(s)	1662, 3228
BsaJI	CCNNGG	7 cut(s)	79, 357, 999, 1532, 2491, 3174, 4236
BsaWI	WCCGGW	3 cut(s)	374, 2944, 3125
Bsc4I	CCNNNNNNNGG	8 cut(s)	85, 363, 1242, 1412, 2466, 3361, 3785, 4279
Bse118I	RCCGGY	2 cut(s)	2944, 3125
Bse1I	ACTGG	7 cut(s)	1080, 2207, 2233, 2770, 3244, 4262, 4273
Bse3DI	GCAATG	5 cut(s)	841, 2254, 2723, 3590, 4124
Bse8I	GATNNNNATC	2 cut(s)	348, 2793
BseBI	CCWGG	5 cut(s)	203, 1000, 1533, 2493, 3186
BseDI	CCNNGG	7 cut(s)	79, 357, 999, 1532, 2491, 3174, 4236
BseJI	GATNNNNATC	2 cut(s)	348, 2793
BseLI	CCNNNNNNNGG	8 cut(s)	85, 363, 1242, 1412, 2466, 3361, 3785, 4279
BseMI	GCAATG	5 cut(s)	841, 2254, 2723, 3590, 4124
BseMII	CTCAG	8 cut(s)	85, 498, 1734, 1797, 3486, 3567, 4148, 4157
BseNI	ACTGG	7 cut(s)	1080, 2207, 2233, 2770, 3244, 4262, 4273
BseRI	GAGGAG	3 cut(s)	10, 13, 3605
BseSI	GKGCMC	4 cut(s)	1075, 2122, 2852, 3385
BsgI	GTGCAG	3 cut(s)	2491, 3340, 4073
Bsh1236I	CGCG	2 cut(s)	230, 501
BshFI	GGCC	4 cut(s)	476, 1167, 1725, 3448
BshNI	GGYRCC	1 cut(s)	3380
BshTI	ACCGGT	2 cut(s)	2944, 3125
BsiHKAI	GWGCWC	7 cut(s)	1075, 1426, 1713, 1756, 2122, 2584, 2852
BsiHKCI	CYCGRG	4 cut(s)	244, 468, 2351, 3520
BsiSI	CCGG	5 cut(s)	375, 2466, 2945, 3037, 3126
BslFI	GGGAC	6 cut(s)	19, 1016, 1928, 2087, 3915, 4206
BslI	CCNNNNNNNGG	8 cut(s)	85, 363, 1242, 1412, 2466, 3361, 3785, 4279
BsmAI	GTCTC	4 cut(s)	792, 1916, 2296, 3651
BsmBI	CGTCTC	2 cut(s)	792, 3651
BsmFI	GGGAC	6 cut(s)	19, 1016, 1928, 2087, 3915, 4206
BsmI	GAATGC	1 cut(s)	1523
BsnI	GGCC	4 cut(s)	476, 1167, 1725, 3448
BsoBI	CYCGRG	4 cut(s)	244, 468, 2351, 3520
Bsp1286I	GDGCHC	8 cut(s)	1075, 1426, 1713, 1756, 2122, 2584, 2852, 3385
Bsp1407I	TGTACA	1 cut(s)	400
Bsp19I	CCATGG	1 cut(s)	3174
BspACI	CCGC	9 cut(s)	561, 1514, 1560, 1604, 2397, 2602, 3278, 3759, 3770
BspANI	GGCC	4 cut(s)	476, 1167, 1725, 3448
BspCNI	CTCAG	8 cut(s)	84, 497, 1735, 1798, 3487, 3568, 4147, 4156
BspFNI	CGCG	2 cut(s)	230, 501
BspHI	TCATGA	1 cut(s)	2917
BspLI	GGNNCC	4 cut(s)	3382, 3754, 3840, 4222
BspMAI	CTGCAG	1 cut(s)	3743
BspMI	ACCTGC	1 cut(s)	2521
BspPI	GGATC	6 cut(s)	344, 992, 1603, 2069, 3833, 3846
BspQI	GCTCTTC	2 cut(s)	159, 402
BspT107I	GGYRCC	1 cut(s)	3380
BsrBI	CCGCTC	1 cut(s)	561
BsrDI	GCAATG	5 cut(s)	841, 2254, 2723, 3590, 4124
BsrFI	RCCGGY	2 cut(s)	2944, 3125
BsrGI	TGTACA	1 cut(s)	400
BsrI	ACTGG	7 cut(s)	1080, 2207, 2233, 2770, 3244, 4262, 4273
BssAI	RCCGGY	2 cut(s)	2944, 3125
BssECI	CCNNGG	7 cut(s)	79, 357, 999, 1532, 2491, 3174, 4236
BssNAI	GTATAC	1 cut(s)	4078
BssNI	GRCGYC	2 cut(s)	1662, 3228
BssSI	CACGAG	1 cut(s)	3209
BssT1I	CCWWGG	2 cut(s)	3174, 4236
Bst1107I	GTATAC	1 cut(s)	4078
Bst2BI	CACGAG	1 cut(s)	3209
Bst2UI	CCWGG	5 cut(s)	203, 1000, 1533, 2493, 3186
Bst6I	CTCTTC	4 cut(s)	40, 159, 402, 2376
BstACI	GRCGYC	2 cut(s)	1662, 3228
BstAPI	GCANNNNNTGC	1 cut(s)	1480
BstAUI	TGTACA	1 cut(s)	400
BstBAI	YACGTR	2 cut(s)	3361, 3968
BstC8I	GCNNGC	2 cut(s)	942, 3551
BstDSI	CCRYGG	1 cut(s)	3174
BstENI	CCTNNNNNAGG	1 cut(s)	1240
BstFNI	CGCG	2 cut(s)	230, 501
BstH2I	RGCGCY	1 cut(s)	415
BstHHI	GCGC	3 cut(s)	232, 414, 1111
BstMAI	GTCTC	4 cut(s)	792, 1916, 2296, 3651
BstMWI	GCNNNNNNNGC	5 cut(s)	1318, 1480, 2469, 3601, 3809
BstNI	CCWGG	5 cut(s)	203, 1000, 1533, 2493, 3186
BstNSI	RCATGY	3 cut(s)	1344, 2608, 3810
BstSCI	CCNGG	6 cut(s)	201, 998, 1531, 2465, 2491, 3184
BstSFI	CTRYAG	4 cut(s)	648, 1540, 2193, 3739
BstSLI	GKGCMC	4 cut(s)	1075, 2122, 2852, 3385
BstUI	CGCG	2 cut(s)	230, 501
BstX2I	RGATCY	2 cut(s)	349, 3838
BstXI	CCANNNNNNTGG	1 cut(s)	3239
BstYI	RGATCY	2 cut(s)	349, 3838
BstZ17I	GTATAC	1 cut(s)	4078
BsuI	GTATCC	2 cut(s)	82, 1239
BsuRI	GGCC	4 cut(s)	476, 1167, 1725, 3448
BtgI	CCRYGG	1 cut(s)	3174
BtsI	GCAGTG	3 cut(s)	2479, 3685, 3748
BveI	ACCTGC	1 cut(s)	2521
Cac8I	GCNNGC	2 cut(s)	942, 3551
CciI	TCATGA	1 cut(s)	2917
CfoI	GCGC	3 cut(s)	232, 414, 1111
Cfr10I	RCCGGY	2 cut(s)	2944, 3125
Cfr13I	GGNCC	6 cut(s)	1165, 1618, 2495, 2599, 3182, 4220
CseI	GACGC	4 cut(s)	1670, 2210, 3217, 3912
Csp6I	GTAC	3 cut(s)	401, 564, 2924
CspAI	ACCGGT	2 cut(s)	2944, 3125
CspCI	CAANNNNNGTGG	2 cut(s)	1813, 1848
CviQI	GTAC	3 cut(s)	401, 564, 2924
DraI	TTTAAA	1 cut(s)	3567
DraIII	CACNNNGTG	1 cut(s)	3691
DriI	GACNNNNNGTC	2 cut(s)	1666, 2605
EaeI	YGGCCR	1 cut(s)	474
Eam1104I	CTCTTC	4 cut(s)	40, 159, 402, 2376
Eam1105I	GACNNNNNGTC	2 cut(s)	1666, 2605
EarI	CTCTTC	4 cut(s)	40, 159, 402, 2376
Ecl136II	GAGCTC	3 cut(s)	1424, 1754, 2582
Eco130I	CCWWGG	2 cut(s)	3174, 4236
Eco24I	GRGCYC	3 cut(s)	1426, 1756, 2584
Eco32I	GATATC	2 cut(s)	1772, 2915
Eco47I	GGWCC	5 cut(s)	1618, 2495, 2599, 3182, 4220
Eco47III	AGCGCT	1 cut(s)	413
Eco53kI	GAGCTC	3 cut(s)	1424, 1754, 2582
Eco57I	CTGAAG	5 cut(s)	1084, 1469, 1918, 3543, 3650
Eco72I	CACGTG	1 cut(s)	3968
Eco88I	CYCGRG	4 cut(s)	244, 468, 2351, 3520
EcoICRI	GAGCTC	3 cut(s)	1424, 1754, 2582
EcoNI	CCTNNNNNAGG	1 cut(s)	1240
EcoO109I	RGGNCCY	2 cut(s)	1165, 3182
EcoRI	GAATTC	2 cut(s)	2274, 3723
EcoRII	CCWGG	5 cut(s)	201, 998, 1531, 2491, 3184
EcoRV	GATATC	2 cut(s)	1772, 2915
EcoT14I	CCWWGG	2 cut(s)	3174, 4236
EcoT22I	ATGCAT	2 cut(s)	1476, 3964
EcoT38I	GRGCYC	3 cut(s)	1426, 1756, 2584
ErhI	CCWWGG	2 cut(s)	3174, 4236
Esp3I	CGTCTC	2 cut(s)	792, 3651
FalI	AAGNNNNNCTT	8 cut(s)	410, 442, 813, 845, 3427, 3459, 3528, 3560
FaqI	GGGAC	6 cut(s)	19, 1016, 1928, 2087, 3915, 4206
FauI	CCCGC	1 cut(s)	3777
FauNDI	CATATG	3 cut(s)	58, 2821, 2959
FbaI	TGATCA	3 cut(s)	745, 1443, 2794
FblI	GTMKAC	2 cut(s)	3388, 4077
FriOI	GRGCYC	3 cut(s)	1426, 1756, 2584
FspAI	RTGCGCAY	1 cut(s)	1110
FspBI	CTAG	5 cut(s)	128, 792, 2280, 2292, 2844
FspI	TGCGCA	1 cut(s)	1110
GlaI	GCGC	3 cut(s)	231, 413, 1110
GsuI	CTGGAG	1 cut(s)	1097
HaeII	RGCGCY	1 cut(s)	415
HaeIII	GGCC	4 cut(s)	476, 1167, 1725, 3448
HapII	CCGG	5 cut(s)	375, 2466, 2945, 3037, 3126
HgaI	GACGC	4 cut(s)	1670, 2210, 3217, 3912
HhaI	GCGC	3 cut(s)	232, 414, 1111
Hin1I	GRCGYC	2 cut(s)	1662, 3228
Hin6I	GCGC	3 cut(s)	230, 412, 1109
HinP1I	GCGC	3 cut(s)	230, 412, 1109
HindIII	AAGCTT	5 cut(s)	723, 1291, 1811, 2681, 2732
HpaII	CCGG	5 cut(s)	375, 2466, 2945, 3037, 3126
HphI	GGTGA	6 cut(s)	95, 139, 1394, 1811, 1988, 2959
Hpy166II	GTNNAC	9 cut(s)	497, 1073, 1819, 1996, 2120, 2850, 2942, 3389, 4078
Hpy8I	GTNNAC	9 cut(s)	497, 1073, 1819, 1996, 2120, 2850, 2942, 3389, 4078
Hpy99I	CGWCG	1 cut(s)	891
HpyCH4IV	ACGT	4 cut(s)	63, 3360, 3609, 3967
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1318, 1480, 2469, 3601, 3809
HpySE526I	ACGT	4 cut(s)	63, 3360, 3609, 3967
Hsp92I	GRCGYC	2 cut(s)	1662, 3228
HspAI	GCGC	3 cut(s)	230, 412, 1109
Ksp22I	TGATCA	3 cut(s)	745, 1443, 2794
LguI	GCTCTTC	2 cut(s)	159, 402
LmnI	GCTCC	5 cut(s)	1078, 1716, 2468, 2579, 3592
LweI	GCATC	8 cut(s)	1120, 1461, 1591, 3289, 3336, 3915, 3971, 4139
MaeI	CTAG	5 cut(s)	128, 792, 2280, 2292, 2844
MaeII	ACGT	4 cut(s)	63, 3360, 3609, 3967
MaeIII	GTNAC	8 cut(s)	95, 1040, 1658, 2508, 2558, 2775, 3972, 4031
MbiI	CCGCTC	1 cut(s)	561
MfeI	CAATTG	7 cut(s)	935, 961, 1010, 1890, 2214, 3255, 3345
MflI	RGATCY	2 cut(s)	349, 3838
MhlI	GDGCHC	8 cut(s)	1075, 1426, 1713, 1756, 2122, 2584, 2852, 3385
MlsI	TGGCCA	1 cut(s)	476
MluNI	TGGCCA	1 cut(s)	476
MlyI	GAGTC	6 cut(s)	460, 521, 1898, 2118, 3512, 3844
MmeI	TCCRAC	5 cut(s)	595, 1070, 2223, 2367, 2604
Mox20I	TGGCCA	1 cut(s)	476
Mph1103I	ATGCAT	2 cut(s)	1476, 3964
MroXI	GAANNNNTTC	7 cut(s)	1097, 1506, 1862, 2055, 2691, 3663, 3708
MscI	TGGCCA	1 cut(s)	476
MslI	CAYNNNNRTG	6 cut(s)	117, 585, 864, 1451, 2876, 4049
Msp20I	TGGCCA	1 cut(s)	476
MspA1I	CMGCKG	1 cut(s)	1562
MspI	CCGG	5 cut(s)	375, 2466, 2945, 3037, 3126
MspR9I	CCNGG	6 cut(s)	203, 1000, 1533, 2467, 2493, 3186
MunI	CAATTG	7 cut(s)	935, 961, 1010, 1890, 2214, 3255, 3345
Mva1269I	GAATGC	1 cut(s)	1523
MvaI	CCWGG	5 cut(s)	203, 1000, 1533, 2493, 3186
MvnI	CGCG	2 cut(s)	230, 501
MwoI	GCNNNNNNNGC	5 cut(s)	1318, 1480, 2469, 3601, 3809
NciI	CCSGG	1 cut(s)	2467
NcoI	CCATGG	1 cut(s)	3174
NdeI	CATATG	3 cut(s)	58, 2821, 2959
NlaIV	GGNNCC	4 cut(s)	3382, 3754, 3840, 4222
NmuCI	GTSAC	3 cut(s)	1658, 2775, 3972
NsbI	TGCGCA	1 cut(s)	1110
NsiI	ATGCAT	2 cut(s)	1476, 3964
NspI	RCATGY	3 cut(s)	1344, 2608, 3810
OliI	CACNNNNGTG	1 cut(s)	1451
PaeR7I	CTCGAG	3 cut(s)	244, 468, 3520
PagI	TCATGA	1 cut(s)	2917
PciSI	GCTCTTC	2 cut(s)	159, 402
PcsI	WCGNNNNNNNCGW	1 cut(s)	3527
PctI	GAATGC	1 cut(s)	1523
PdmI	GAANNNNTTC	7 cut(s)	1097, 1506, 1862, 2055, 2691, 3663, 3708
PflFI	GACNNNGTC	1 cut(s)	3643
PflMI	CCANNNNNTGG	1 cut(s)	4279
PfoI	TCCNGGA	1 cut(s)	201
PinAI	ACCGGT	2 cut(s)	2944, 3125
PleI	GAGTC	6 cut(s)	460, 520, 1898, 2118, 3512, 3844
PmaCI	CACGTG	1 cut(s)	3968
PmlI	CACGTG	1 cut(s)	3968
PpsI	GAGTC	6 cut(s)	460, 520, 1898, 2118, 3512, 3844
Ppu21I	YACGTR	2 cut(s)	3361, 3968
PpuMI	RGGWCCY	1 cut(s)	3182
PsiI	TTATAA	1 cut(s)	992
Psp124BI	GAGCTC	3 cut(s)	1426, 1756, 2584
Psp5II	RGGWCCY	1 cut(s)	3182
Psp6I	CCWGG	5 cut(s)	201, 998, 1531, 2491, 3184
PspCI	CACGTG	1 cut(s)	3968
PspGI	CCWGG	5 cut(s)	201, 998, 1531, 2491, 3184
PspN4I	GGNNCC	4 cut(s)	3382, 3754, 3840, 4222
PspPI	GGNCC	6 cut(s)	1165, 1618, 2495, 2599, 3182, 4220
PspPPI	RGGWCCY	1 cut(s)	3182
PspXI	VCTCGAGB	2 cut(s)	468, 3520
PsrI	GAACNNNNNNTAC	2 cut(s)	4184, 4216
PstI	CTGCAG	1 cut(s)	3743
PsuI	RGATCY	2 cut(s)	349, 3838
PsyI	GACNNNGTC	1 cut(s)	3643
RsaI	GTAC	3 cut(s)	402, 565, 2925
RsaNI	GTAC	3 cut(s)	401, 564, 2924
RseI	CAYNNNNRTG	6 cut(s)	117, 585, 864, 1451, 2876, 4049
SacI	GAGCTC	3 cut(s)	1426, 1756, 2584
SapI	GCTCTTC	2 cut(s)	159, 402
Sau96I	GGNCC	6 cut(s)	1165, 1618, 2495, 2599, 3182, 4220
SchI	GAGTC	6 cut(s)	460, 521, 1898, 2118, 3512, 3844
ScrFI	CCNGG	6 cut(s)	203, 1000, 1533, 2467, 2493, 3186
SduI	GDGCHC	8 cut(s)	1075, 1426, 1713, 1756, 2122, 2584, 2852, 3385
SfaNI	GCATC	8 cut(s)	1120, 1461, 1591, 3289, 3336, 3915, 3971, 4139
SfcI	CTRYAG	4 cut(s)	648, 1540, 2193, 3739
Sfr274I	CTCGAG	3 cut(s)	244, 468, 3520
SinI	GGWCC	5 cut(s)	1618, 2495, 2599, 3182, 4220
SlaI	CTCGAG	3 cut(s)	244, 468, 3520
SmiMI	CAYNNNNRTG	6 cut(s)	117, 585, 864, 1451, 2876, 4049
SmlI	CTYRAG	7 cut(s)	244, 468, 1218, 1632, 1952, 2126, 3520
SmoI	CTYRAG	7 cut(s)	244, 468, 1218, 1632, 1952, 2126, 3520
SsiI	CCGC	9 cut(s)	561, 1514, 1560, 1604, 2397, 2602, 3278, 3759, 3770
SspI	AATATT	3 cut(s)	1326, 2266, 2989
SspMI	CTAG	5 cut(s)	128, 792, 2280, 2292, 2844
SstI	GAGCTC	3 cut(s)	1426, 1756, 2584
StyD4I	CCNGG	6 cut(s)	201, 998, 1531, 2465, 2491, 3184
StyI	CCWWGG	2 cut(s)	3174, 4236
TaiI	ACGT	4 cut(s)	66, 3363, 3612, 3970
TaqII	GACCGA	2 cut(s)	147, 2512
TatI	WGTACW	1 cut(s)	400
TseFI	GTSAC	3 cut(s)	1658, 2775, 3972
Tsp45I	GTSAC	3 cut(s)	1658, 2775, 3972
TspGWI	ACGGA	1 cut(s)	2416
Tth111I	GACNNNGTC	1 cut(s)	3643
Van91I	CCANNNNNTGG	1 cut(s)	4279
VneI	GTGCAC	3 cut(s)	1071, 2118, 2848
VpaK11BI	GGWCC	5 cut(s)	1618, 2495, 2599, 3182, 4220
XagI	CCTNNNNNAGG	1 cut(s)	1240
XapI	RAATTY	9 cut(s)	2097, 2172, 2274, 2346, 2551, 3115, 3723, 4097, 4248
XbaI	TCTAGA	2 cut(s)	127, 791
XceI	RCATGY	3 cut(s)	1344, 2608, 3810
XhoI	CTCGAG	3 cut(s)	244, 468, 3520
XmiI	GTMKAC	2 cut(s)	3388, 4077
XmnI	GAANNNNTTC	7 cut(s)	1097, 1506, 1862, 2055, 2691, 3663, 3708
XspI	CTAG	5 cut(s)	128, 792, 2280, 2292, 2844
Zsp2I	ATGCAT	2 cut(s)	1476, 3964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.