Rh5CG528800

NAD(P)H-quinone oxidoreductase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013396
Physical Location & Seq
Forward (+)
73946238 .. 73947237
1000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG528800.1

Sequence Viewer

Length: 672 bp
ATGGCAACGGCTGCAAATTGCTTAAGGCATGGGACTGTACCACTCTCGGCCAACCTCCAGCACCTGCAGCCTCGAAGAAACTACTGCATGGTAAACATGGCGGGAAACAGAAACATGAAGCTGATGGGTGGACATGGCAAAGCATCCAAGAGGAGTGGGATGATAACGTGTGCAGGGAGGGAGATAGCAGACTTCATAGGAGGGGATTTGGTGAGATTTGATCTGGGACAGTGGCTTTCCGATGTGGAAGAGCACAAGGCCCTCGCAGTCTACTCACCCCACGAGGGAGGCTACGAGGGCCGCTACCTTAACCGATTGACATACCAGGGTTACTATTTCCTGGACCTCAGCGCCCGAGGTCTTGGTGATCCTGAGACCACTCTCACCAAGGTCCATCCAGTTTGCCCGGCACATTTAGGGAAGCAGCCGATAGCAAGATGGTATTTTCCACCAGAGGTTGATTATAGGCTTGCTGCGCTGCCTCCAAAGGCCAAAGGGCTTGTGGTCTGGATTATTGAAGCCAAGGTTCTGTCAAAGGCAGAATTGCAGTTTCTTGCGCTCCTTCCAACCCTCCGCCCCAAAGTCAGGGTCATTGCTGAGTGCGGAAACTGGAGAAGATTCGTGTGGAAGCCACTCAAGGAGATTTCGGGACTAACTGAAAGTGAGGAATGA

Protein Analysis

223

Amino Acids

24.99

Weight (kDa)

9.3

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhN PF11909 77 - 214 5.1e-55 NADH-quinone oxidoreductase cyanobacterial subunit N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58260 AT5G58260
fragaria_vesca FvH4_3g41140
malus_domestica MD03G1047200.v1.1 MD11G1049700.v1.1
prunus_persica Prupe.6G038400_v2.0.a1
pyrus_communis pycom03g03720 pycom11g04030
rosa_chinensis RchiOBHm_Chr5g0073911
rosa_laevigata RLG00000036424
rosa_multiflora Rmu_sc0000470.1_g000017
rosa_roxburghii Rroxscaffold_1G00007460
rosa_rugosa Rorug05G0427300
rosa_samantha Rh5AG484300 Rh5BG505000 Rh5CG528800 Rh5DG517300
rosa_wichuraiana Rw5G045020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 72
Acc36I ACCTGC 1 cut(s) 72
AccI GTMKAC 1 cut(s) 270
AciI CCGC 4 cut(s) 101, 301, 574, 603
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 48
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 2 cut(s) 284, 585
AflII CTTAAG 1 cut(s) 22
AflIII ACRYGT 1 cut(s) 167
AgsI TTSAA 1 cut(s) 518
AjnI CCWGG 2 cut(s) 324, 339
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 354
AoxI GGCC 4 cut(s) 48, 258, 298, 489
ApeKI GCWGC 5 cut(s) 11, 67, 424, 473, 478
ArsI GACNNNNNNTTYG 2 cut(s) 573, 605
AspLEI GCGC 3 cut(s) 353, 478, 559
AspS9I GGNCC 4 cut(s) 259, 298, 343, 391
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 4 cut(s) 223, 267, 376, 377
AvaI CYCGRG 1 cut(s) 354
AvaII GGWCC 2 cut(s) 343, 391
BauI CACGAG 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 255
BbvCI CCTCAGC 1 cut(s) 347
BbvI GCAGC 4 cut(s) 79, 436, 460, 465
BccI CCATC 3 cut(s) 118, 402, 432
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 2 cut(s) 326, 341
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 65
BfoI RGCGCY 1 cut(s) 354
BfrI CTTAAG 1 cut(s) 22
BfuAI ACCTGC 1 cut(s) 72
BisI GCNGC 6 cut(s) 12, 68, 301, 425, 474, 479
BlsI GCNGC 6 cut(s) 13, 69, 302, 426, 475, 480
Bme1390I CCNGG 3 cut(s) 326, 341, 407
Bme18I GGWCC 2 cut(s) 343, 391
BmeT110I CYCGRG 1 cut(s) 354
BmgT120I GGNCC 4 cut(s) 259, 298, 343, 391
BmrFI CCNGG 3 cut(s) 326, 341, 407
BmsI GCATC 1 cut(s) 152
BpmI CTGGAG 2 cut(s) 41, 631
Bpu10I CCTNAGC 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 620
BpuMI CCSGG 1 cut(s) 407
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 4 cut(s) 325, 355, 387, 522
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 585
Bse1I ACTGG 2 cut(s) 398, 614
Bse3DI GCAATG 1 cut(s) 591
BseBI CCWGG 2 cut(s) 326, 341
BseDI CCNNGG 4 cut(s) 325, 355, 387, 522
BseGI GGATG 3 cut(s) 143, 165, 394
BseLI CCNNNNNNNGG 2 cut(s) 284, 585
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 3 cut(s) 361, 363, 588
BseNI ACTGG 2 cut(s) 398, 614
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 4 cut(s) 79, 436, 460, 465
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 4 cut(s) 50, 260, 300, 491
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 1 cut(s) 407
BslFI GGGAC 3 cut(s) 46, 240, 663
BslI CCNNNNNNNGG 2 cut(s) 284, 585
BsmAI GTCTC 1 cut(s) 368
BsmFI GGGAC 3 cut(s) 46, 240, 663
BsnI GGCC 4 cut(s) 50, 260, 300, 491
Bso31I GGTCTC 1 cut(s) 368
BsoBI CYCGRG 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 220, 367
BspACI CCGC 4 cut(s) 101, 301, 574, 603
BspANI GGCC 4 cut(s) 50, 260, 300, 491
BspCNI CTCAG 3 cut(s) 360, 364, 589
BspMAI CTGCAG 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 72
BspPI GGATC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 243
BspTI CTTAAG 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 591
BsrI ACTGG 2 cut(s) 398, 614
BssECI CCNNGG 4 cut(s) 325, 355, 387, 522
BssMI GATC 2 cut(s) 220, 367
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 387, 522
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 2 cut(s) 326, 341
Bst4CI ACNGT 2 cut(s) 37, 231
Bst6I CTCTTC 1 cut(s) 243
BstAFI CTTAAG 1 cut(s) 22
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 3 cut(s) 347, 372, 597
BstF5I GGATG 3 cut(s) 143, 165, 394
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 3 cut(s) 353, 478, 559
BstKTI GATC 2 cut(s) 223, 370
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 2 cut(s) 220, 367
BstMWI GCNNNNNNNGC 4 cut(s) 11, 67, 297, 475
BstNI CCWGG 2 cut(s) 326, 341
BstSCI CCNGG 3 cut(s) 324, 339, 405
BstSFI CTRYAG 1 cut(s) 65
BstV1I GCAGC 4 cut(s) 79, 436, 460, 465
BsuRI GGCC 4 cut(s) 50, 260, 300, 491
BtsCI GGATG 3 cut(s) 143, 165, 394
BtsIMutI CAGTG 1 cut(s) 236
BveI ACCTGC 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 471
CaiI CAGNNNCTG 1 cut(s) 64
CfoI GCGC 3 cut(s) 353, 478, 559
Cfr13I GGNCC 4 cut(s) 259, 298, 343, 391
Csp6I GTAC 1 cut(s) 38
CspCI CAANNNNNGTGG 2 cut(s) 136, 171
CviAII CATG 5 cut(s) 29, 88, 97, 115, 134
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 3 cut(s) 347, 372, 597
DpnI GATC 2 cut(s) 222, 369
DpnII GATC 2 cut(s) 220, 367
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
EciI GGCGGA 1 cut(s) 563
Eco130I CCWWGG 2 cut(s) 387, 522
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 2 cut(s) 343, 391
Eco88I CYCGRG 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 324, 339
EcoT14I CCWWGG 2 cut(s) 387, 522
ErhI CCWWGG 2 cut(s) 387, 522
FaeI CATG 5 cut(s) 32, 91, 100, 118, 137
FaiI YATR 8 cut(s) 30, 89, 98, 116, 135, 197, 322, 465
FaqI GGGAC 3 cut(s) 46, 240, 663
FatI CATG 5 cut(s) 28, 87, 96, 114, 133
FauI CCCGC 1 cut(s) 94
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 6 cut(s) 12, 68, 301, 425, 474, 479
FokI GGATG 3 cut(s) 130, 172, 381
Fsp4HI GCNGC 6 cut(s) 12, 68, 301, 425, 474, 479
GlaI GCGC 3 cut(s) 352, 477, 558
GluI GCNGC 6 cut(s) 12, 68, 301, 425, 474, 479
GsuI CTGGAG 2 cut(s) 41, 631
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 4 cut(s) 50, 260, 300, 491
HapII CCGG 1 cut(s) 407
HhaI GCGC 3 cut(s) 353, 478, 559
Hin1II CATG 5 cut(s) 32, 91, 100, 118, 137
Hin6I GCGC 3 cut(s) 351, 476, 557
HinP1I GCGC 3 cut(s) 351, 476, 557
HinfI GANTC 1 cut(s) 618
HpaII CCGG 1 cut(s) 407
HphI GGTGA 4 cut(s) 223, 267, 376, 377
Hpy166II GTNNAC 3 cut(s) 94, 131, 271
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 3 cut(s) 371, 508, 648
Hpy8I GTNNAC 3 cut(s) 94, 131, 271
HpyAV CCTTC 1 cut(s) 572
HpyCH4III ACNGT 2 cut(s) 37, 231
HpyCH4IV ACGT 1 cut(s) 167
HpyCH4V TGCA 5 cut(s) 14, 67, 87, 173, 547
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 67, 297, 475
HpyF3I CTNAG 3 cut(s) 347, 372, 597
HpySE526I ACGT 1 cut(s) 167
Hsp92II CATG 5 cut(s) 32, 91, 100, 118, 137
HspAI GCGC 3 cut(s) 351, 476, 557
Kzo9I GATC 2 cut(s) 220, 367
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 4 cut(s) 79, 436, 460, 465
LweI GCATC 1 cut(s) 152
MaeII ACGT 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 329
MalI GATC 2 cut(s) 222, 369
MboI GATC 2 cut(s) 220, 367
MboII GAAGA 3 cut(s) 87, 260, 627
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 2 cut(s) 16, 542
MmeI TCCRAC 1 cut(s) 590
MseI TTAA 2 cut(s) 23, 309
MspCI CTTAAG 1 cut(s) 22
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 3 cut(s) 326, 341, 407
MvaI CCWGG 2 cut(s) 326, 341
MwoI GCNNNNNNNGC 4 cut(s) 11, 67, 297, 475
NciI CCSGG 1 cut(s) 407
NdeII GATC 2 cut(s) 220, 367
NlaIII CATG 5 cut(s) 32, 91, 100, 118, 137
NmeAIII GCCGAG 1 cut(s) 26
PaqCI CACCTGC 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 243
PfeI GAWTC 1 cut(s) 618
PfoI TCCNGGA 1 cut(s) 339
PkrI GCNGC 6 cut(s) 13, 69, 302, 426, 475, 480
Psp6I CCWGG 2 cut(s) 324, 339
PspGI CCWGG 2 cut(s) 324, 339
PspPI GGNCC 4 cut(s) 259, 298, 343, 391
PstI CTGCAG 1 cut(s) 69
PstNI CAGNNNCTG 1 cut(s) 64
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 23, 309
SatI GCNGC 6 cut(s) 12, 68, 301, 425, 474, 479
Sau3AI GATC 2 cut(s) 220, 367
Sau96I GGNCC 4 cut(s) 259, 298, 343, 391
ScrFI CCNGG 3 cut(s) 326, 341, 407
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 1 cut(s) 152
SfcI CTRYAG 1 cut(s) 65
SinI GGWCC 2 cut(s) 343, 391
SmlI CTYRAG 2 cut(s) 22, 635
SmoI CTYRAG 2 cut(s) 22, 635
Sse9I AATT 2 cut(s) 16, 542
SsiI CCGC 4 cut(s) 101, 301, 574, 603
StyD4I CCNGG 3 cut(s) 324, 339, 405
StyI CCWWGG 2 cut(s) 387, 522
TaaI ACNGT 2 cut(s) 37, 231
TaiI ACGT 1 cut(s) 170
TaqI TCGA 1 cut(s) 73
TasI AATT 2 cut(s) 16, 542
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 1 cut(s) 618
Tru1I TTAA 2 cut(s) 23, 309
Tru9I TTAA 2 cut(s) 23, 309
TscAI CASTG 1 cut(s) 236
TseI GCWGC 5 cut(s) 11, 67, 424, 473, 478
TspDTI ATGAA 2 cut(s) 131, 184
TspRI CASTG 1 cut(s) 236
Vha464I CTTAAG 1 cut(s) 22
VpaK11BI GGWCC 2 cut(s) 343, 391
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.