Rorug06G0069200

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007290
Physical Location & Seq
Reverse (-)
9219065 .. 9219542
478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0069200.1

Sequence Viewer

Length: 393 bp
ATGGACCGGGATTACTCTAATTCCTTGTCCGGTGCCATCGAAGCTTTGAGGGCTAGCACGCTCCGCCTTCTAGTGGTTGATGGAGCAAGGGCAGATGTCCACAATGTGAAGGATGCTATTTCTTCCGCAATTGATGTGATGCAGGCAATTGCATCATCAATATGCTTATTATTATCAAAGGTGGTTTTTCTCAAACTTTATGGTACTGGCCAAGTCAATTGCCAAGTCGCTGTCAATTACATATCATTGGATAGGTCACGTTGCATGTTACTGATCAAGTCACTGCCTATGTCACTAACCAACGACGTCACTGGCCATCCCAGGTCCCTAGTTAGTGAGACCACTGGCCAAGTCACTGTTAACCTCAGGTCACTGGCCATATTACAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

130

Amino Acids

13.98

Weight (kDa)

8.55

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 1 - 61 7.1e-22 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 32
AciI CCGC 2 cut(s) 64, 126
AcoI YGGCCR 4 cut(s) 208, 313, 346, 375
AcyI GRCGYC 1 cut(s) 306
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 73
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 1 cut(s) 44
AluI AGCT 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 332
AoxI GGCC 4 cut(s) 208, 313, 346, 375
AspS9I GGNCC 2 cut(s) 4, 324
AsuC2I CCSGG 1 cut(s) 8
AsuNHI GCTAGC 1 cut(s) 53
AvaII GGWCC 2 cut(s) 4, 324
AxyI CCTNAGG 1 cut(s) 365
BalI TGGCCA 4 cut(s) 210, 315, 348, 377
BanI GGYRCC 1 cut(s) 32
BccI CCATC 3 cut(s) 44, 74, 324
BciT130I CCWGG 1 cut(s) 322
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 1 cut(s) 8
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 3 cut(s) 54, 71, 329
Bme1390I CCNGG 2 cut(s) 8, 322
Bme18I GGWCC 2 cut(s) 4, 324
BmgT120I GGNCC 2 cut(s) 4, 324
BmiI GGNNCC 2 cut(s) 34, 326
BmrFI CCNGG 2 cut(s) 8, 322
BmsI GCATC 3 cut(s) 103, 129, 161
BmtI GCTAGC 1 cut(s) 57
BpuMI CCSGG 1 cut(s) 8
BsaHI GRCGYC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 1 cut(s) 320
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 4 cut(s) 211, 316, 349, 378
Bse21I CCTNAGG 1 cut(s) 365
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 2 cut(s) 118, 316
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 379
BseNI ACTGG 4 cut(s) 211, 316, 349, 378
BshFI GGCC 4 cut(s) 210, 315, 348, 377
BshNI GGYRCC 1 cut(s) 32
BsiSI CCGG 2 cut(s) 7, 30
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 4 cut(s) 210, 315, 348, 377
Bso31I GGTCTC 1 cut(s) 332
Bsp143I GATC 1 cut(s) 273
BspACI CCGC 2 cut(s) 64, 126
BspANI GGCC 4 cut(s) 210, 315, 348, 377
BspCNI CTCAG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 389
BspLI GGNNCC 2 cut(s) 34, 326
BspOI GCTAGC 1 cut(s) 57
BspT107I GGYRCC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 332
BsrI ACTGG 4 cut(s) 211, 316, 349, 378
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 1 cut(s) 273
BssNI GRCGYC 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 1 cut(s) 358
BstACI GRCGYC 1 cut(s) 306
BstC8I GCNNGC 3 cut(s) 55, 59, 144
BstDEI CTNAG 1 cut(s) 365
BstF5I GGATG 2 cut(s) 118, 316
BstKTI GATC 1 cut(s) 276
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 1 cut(s) 273
BstMWI GCNNNNNNNGC 3 cut(s) 41, 50, 63
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 2 cut(s) 6, 320
Bsu36I CCTNAGG 1 cut(s) 365
BsuRI GGCC 4 cut(s) 210, 315, 348, 377
BtsCI GGATG 2 cut(s) 118, 316
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 5 cut(s) 281, 309, 342, 354, 371
Cac8I GCNNGC 3 cut(s) 55, 59, 144
CciI TCATGA 1 cut(s) 389
Cfr13I GGNCC 2 cut(s) 4, 324
Csp6I GTAC 1 cut(s) 204
CviAII CATG 2 cut(s) 265, 390
CviJI RGCY 6 cut(s) 44, 53, 210, 315, 348, 377
CviKI_1 RGCY 6 cut(s) 44, 53, 210, 315, 348, 377
CviQI GTAC 1 cut(s) 204
DdeI CTNAG 1 cut(s) 365
DpnI GATC 1 cut(s) 275
DpnII GATC 1 cut(s) 273
DraIII CACNNNGTG 1 cut(s) 106
EaeI YGGCCR 4 cut(s) 208, 313, 346, 375
EciI GGCGGA 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 332
Eco47I GGWCC 2 cut(s) 4, 324
Eco81I CCTNAGG 1 cut(s) 365
EcoO109I RGGNCCY 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 320
FaeI CATG 2 cut(s) 268, 393
FaiI YATR 7 cut(s) 163, 201, 242, 266, 290, 380, 391
FaqI GGGAC 1 cut(s) 310
FatI CATG 2 cut(s) 264, 389
FbaI TGATCA 1 cut(s) 273
FokI GGATG 2 cut(s) 125, 303
FspBI CTAG 3 cut(s) 54, 71, 329
HaeIII GGCC 4 cut(s) 210, 315, 348, 377
HapII CCGG 2 cut(s) 7, 30
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 2 cut(s) 268, 393
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 42
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 2 cut(s) 7, 30
Hpy166II GTNNAC 2 cut(s) 100, 361
Hpy188III TCNNGA 1 cut(s) 390
Hpy8I GTNNAC 2 cut(s) 100, 361
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 2 cut(s) 77, 103
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4IV ACGT 2 cut(s) 259, 306
HpyCH4V TGCA 3 cut(s) 142, 152, 264
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 50, 63
HpyF3I CTNAG 1 cut(s) 365
HpySE526I ACGT 2 cut(s) 259, 306
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 2 cut(s) 268, 393
Ksp22I TGATCA 1 cut(s) 273
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 1 cut(s) 273
LmnI GCTCC 2 cut(s) 66, 83
LweI GCATC 3 cut(s) 103, 129, 161
MaeI CTAG 3 cut(s) 54, 71, 329
MaeII ACGT 2 cut(s) 259, 306
MaeIII GTNAC 7 cut(s) 255, 267, 279, 291, 307, 352, 369
MalI GATC 1 cut(s) 275
MboI GATC 1 cut(s) 273
MboII GAAGA 1 cut(s) 114
MfeI CAATTG 3 cut(s) 129, 147, 217
MlsI TGGCCA 4 cut(s) 210, 315, 348, 377
MluCI AATT 5 cut(s) 19, 129, 147, 217, 235
MluNI TGGCCA 4 cut(s) 210, 315, 348, 377
MnlI CCTC 2 cut(s) 42, 374
Mox20I TGGCCA 4 cut(s) 210, 315, 348, 377
MscI TGGCCA 4 cut(s) 210, 315, 348, 377
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 160
Msp20I TGGCCA 4 cut(s) 210, 315, 348, 377
MspI CCGG 2 cut(s) 7, 30
MspR9I CCNGG 2 cut(s) 8, 322
MunI CAATTG 3 cut(s) 129, 147, 217
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 3 cut(s) 41, 50, 63
NciI CCSGG 1 cut(s) 8
NdeII GATC 1 cut(s) 273
NheI GCTAGC 1 cut(s) 53
NlaIII CATG 2 cut(s) 268, 393
NlaIV GGNNCC 2 cut(s) 34, 326
NmuCI GTSAC 6 cut(s) 255, 279, 291, 307, 352, 369
NspI RCATGY 1 cut(s) 268
PagI TCATGA 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 2 cut(s) 34, 326
PspPI GGNCC 2 cut(s) 4, 324
PspPPI RGGWCCY 1 cut(s) 324
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
RseI CAYNNNNRTG 1 cut(s) 160
SaqAI TTAA 1 cut(s) 360
Sau3AI GATC 1 cut(s) 273
Sau96I GGNCC 2 cut(s) 4, 324
ScrFI CCNGG 2 cut(s) 8, 322
SetI ASST 8 cut(s) 46, 183, 257, 262, 309, 326, 366, 371
SfaNI GCATC 3 cut(s) 103, 129, 161
SinI GGWCC 2 cut(s) 4, 324
SmiMI CAYNNNNRTG 1 cut(s) 160
Sse9I AATT 5 cut(s) 19, 129, 147, 217, 235
SsiI CCGC 2 cut(s) 64, 126
SspMI CTAG 3 cut(s) 54, 71, 329
StyD4I CCNGG 2 cut(s) 6, 320
TaaI ACNGT 1 cut(s) 358
TaiI ACGT 2 cut(s) 262, 309
TaqI TCGA 1 cut(s) 39
TasI AATT 5 cut(s) 19, 129, 147, 217, 235
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 5 cut(s) 288, 316, 349, 361, 378
TseFI GTSAC 6 cut(s) 255, 279, 291, 307, 352, 369
Tsp45I GTSAC 6 cut(s) 255, 279, 291, 307, 352, 369
TspRI CASTG 5 cut(s) 288, 316, 349, 361, 378
VpaK11BI GGWCC 2 cut(s) 4, 324
XceI RCATGY 1 cut(s) 268
XspI CTAG 3 cut(s) 54, 71, 329
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.