Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0009526

Physical Location & Seq

Genomic Coordinates Forward (+)

58844934 .. 58846426

Gene Structure 1493 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0463000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGGATGGCCAACTTGGTGTAAATGGAGAAAATTCTCTAGTACCCTGCTTGCTAGAGCGATTCCTTGAGTTGGGCTCTGGAACGAGGAGTGAAGTGCCACTACAGGTTTGTTCTGTTAAGGCTGGAGGAATGATGTCCCTAGCAATAGATAATCTCGGTACCCTTTGGATGTGGGGCAATTGTCCACTAGAAAGCAGCAGCAGTGAAGGTGGGTTCTCTCTTGTGAGCAGTTTCACTCCAACCCCAGTGTGGGATTTCCATGGCCACATGGTTGTGAAGGTAGCATGCGGAAATGAGCATGTAGTAGCCCTAGTCACGGCTGGAGAAACATATAAAGGTGAAGATCTTATATGCTTCTCTTGGGGTAGTAACAATCATGGGCAGTTAGGCCTCGGGGATAAAGAGAACCGGAGTCGTCCTGAAATAGTAGAAGATTTTAACAAGGACTCCATATTTGCGGTTTATGAAGTAGCATGTGGGGCTTTTCACACAGCTGTGCTTACCCACAAGAAAAGACCAAGTGAGACATTAGAAAGCATGTGCTGGACGTTTGGCCTTGGAGAAAATGGGCAACTTGGGCATGGAACCACCCAAAGTGCCTCATCTCCTGAAACTGTGAAAGAATTGCCACAATATGTGTATCTGATCTCTGTTGACTGTGGCTTATTCCACACTAGTGTTGTTTCATCTGCAGGAGATGTGTGGTCATGGGGGATGGAGAAGGGTCTCGGTCTATGCCCAGATGCTAGTTTTACTGGGACAGATGCAGGGGATGCCATTTCTCCCTTACAGATTCTGTGTAACGGATCCAATGGGTCTAAATTTCAGGAGCCGGTCCAAGTTGTATGTGGTGCTGCCCATACTGTTCTTGTAGCAAATGATGGATATAAGCTTTGGTCTTGGGGGAGAGGAAGAAGTGGGGTTCTCGGAAATGGCAAGACAATCGACTGTTTTTCTCCCACTGTTGTATTGTGGCCTCCACTTACAGAGGATTTCAAGCAAGAAGAACTAAATACTGTTGGGGAGGGAGACAGAATTGCGGAAGAGAATTCTAAAGGAGTCACCAAAACAGAAAAAGCCTTGTGTTCAGCAATTGAAGAAACGAAACTTCTTCAATCAAAACTCGCAGTGATGGAACGATATGCTGACATACTTCATGGTTCAATATTTGGAAAACCCTTGGAAGAGCAGGATATTCCAATCTCCTTGCAGAACTCAGGTATTCATGACATTGGAAAGGAATGGGACAGAATGCTAGAGTCAGCAGATCCTGCGAAGCTCGTAAGGTTGGAAAAGTTCTATCAAAAAATGCTTGAAGGTGTTAAAGAAAAGCTGTTGAAGAAAAGGATTCAGGAGATAGTAAAAGAGTGTCTCAGTTCTACTATAAACAAAGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000469 GO:0000470 GO:0001522 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016073 GO:0019538 GO:0022613 GO:0030515 GO:0031118 GO:0031120 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034513 GO:0034641 GO:0034645 GO:0034660 GO:0040031 GO:0042254 GO:0042273 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Ribosomal_L7Ae

Protein Analysis

465

Amino Acids

50.52

Weight (kDa)

5.08

Isoelectric Point (pI)

40.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_RLD	PF25390	2 - 324	1.3e-46	RCC1-like domain
RCC1_2	PF13540	91 - 128	1.3e-06	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	118 - 167	1.1e-12	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	181 - 227	3.1e-09	Regulator of chromosome condensation (RCC1) repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009526)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08180 AT5G08180
fragaria_vesca	FvH4_5g13030
malus_domestica	MD06G1207900.v1.1 MD14G1218300.v1.1
prunus_persica	Prupe.5G212100_v2.0.a1
pyrus_communis	pycom06g18580 pycom14g18100
rosa_chinensis	RchiOBHm_Chr7g0184101
rosa_laevigata	RLG00000005000 RLG00000021127
rosa_multiflora	Rmu_sc0004712.1_g000005 Rmu_sc0004712.1_g000006 Rmu_sc0004712.1_g000007 Rmu_sc0025350.1_g000010
rosa_roxburghii	Rroxscaffold_3G00269880
rosa_rugosa	Rorug06G0463000
rosa_samantha	Rh7AG082800 Rh7BG064200 Rh7DG064400
rosa_wichuraiana	Rw0G002480 Rw7G005280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	158
AccB1I	GGYRCC	1 cut(s)	158
AciI	CCGC	3 cut(s)	288, 458, 1040
AclWI	GGATC	3 cut(s)	801, 814, 1262
AcoI	YGGCCR	2 cut(s)	7, 262
AcsI	RAATTY	3 cut(s)	31, 821, 1048
AfaI	GTAC	2 cut(s)	42, 160
AfiI	CCNNNNNNNGG	4 cut(s)	70, 249, 250, 316
AgsI	TTSAA	6 cut(s)	997, 1097, 1115, 1164, 1316, 1339
AhlI	ACTAGT	1 cut(s)	674
AleI	CACNNNNGTG	2 cut(s)	494, 675
AluBI	AGCT	4 cut(s)	494, 892, 1279, 1333
AluI	AGCT	4 cut(s)	494, 892, 1279, 1333
Alw26I	GTCTC	4 cut(s)	518, 731, 1023, 1376
AlwI	GGATC	3 cut(s)	801, 814, 1262
AlwNI	CAGNNNCTG	2 cut(s)	796, 1271
Ama87I	CYCGRG	1 cut(s)	392
AoxI	GGCC	5 cut(s)	7, 262, 388, 553, 974
ApeKI	GCWGC	3 cut(s)	195, 198, 854
ApoI	RAATTY	3 cut(s)	31, 821, 1048
ArsI	GACNNNNNNTTYG	2 cut(s)	437, 469
Asp718I	GGTACC	1 cut(s)	158
AspS9I	GGNCC	1 cut(s)	835
AsuHPI	GGTGA	2 cut(s)	350, 1054
AvaI	CYCGRG	1 cut(s)	392
AvaII	GGWCC	1 cut(s)	835
BalI	TGGCCA	2 cut(s)	9, 264
BamHI	GGATCC	1 cut(s)	806
BanI	GGYRCC	1 cut(s)	158
BanII	GRGCYC	1 cut(s)	77
BarI	GAAGNNNNNNTAC	2 cut(s)	84, 116
BbvI	GCAGC	3 cut(s)	207, 210, 841
BccI	CCATC	3 cut(s)	709, 875, 1126
BceAI	ACGGC	1 cut(s)	333
BcgI	CGANNNNNNTGC	2 cut(s)	925, 959
BcoDI	GTCTC	4 cut(s)	518, 731, 1023, 1376
BcuI	ACTAGT	1 cut(s)	674
BfaI	CTAG	8 cut(s)	38, 53, 140, 188, 311, 675, 747, 1256
BfmI	CTRYAG	2 cut(s)	101, 690
BglII	AGATCT	1 cut(s)	343
BisI	GCNGC	3 cut(s)	196, 199, 855
BlsI	GCNGC	3 cut(s)	197, 200, 856
Bme18I	GGWCC	1 cut(s)	835
BmeT110I	CYCGRG	1 cut(s)	392
BmgT120I	GGNCC	1 cut(s)	835
BmiI	GGNNCC	4 cut(s)	160, 586, 808, 831
BmrI	ACTGGG	2 cut(s)	239, 765
BmsI	GCATC	3 cut(s)	733, 754, 763
BmuI	ACTGGG	2 cut(s)	239, 765
BplI	GAGNNNNNCTC	2 cut(s)	59, 91
BpmI	CTGGAG	2 cut(s)	144, 342
BpuEI	CTTGAG	1 cut(s)	86
BsaI	GGTCTC	1 cut(s)	731
BsaJI	CCNNGG	4 cut(s)	259, 391, 556, 1179
BsaWI	WCCGGW	1 cut(s)	408
BsaXI	ACNNNNNCTCC	2 cut(s)	431, 461
Bsc4I	CCNNNNNNNGG	4 cut(s)	70, 249, 250, 316
Bse118I	RCCGGY	1 cut(s)	832
Bse1I	ACTGG	2 cut(s)	245, 760
BseDI	CCNNGG	4 cut(s)	259, 391, 556, 1179
BseGI	GGATG	4 cut(s)	10, 174, 720, 778
BseLI	CCNNNNNNNGG	4 cut(s)	70, 249, 250, 316
BseMII	CTCAG	2 cut(s)	1230, 1387
BseNI	ACTGG	2 cut(s)	245, 760
BseRI	GAGGAG	1 cut(s)	100
BseXI	GCAGC	3 cut(s)	207, 210, 841
BshFI	GGCC	5 cut(s)	9, 264, 390, 555, 976
BshNI	GGYRCC	1 cut(s)	158
BsiHKCI	CYCGRG	1 cut(s)	392
BsiSI	CCGG	2 cut(s)	409, 833
BslFI	GGGAC	3 cut(s)	121, 772, 1259
BslI	CCNNNNNNNGG	4 cut(s)	70, 249, 250, 316
BsmAI	GTCTC	4 cut(s)	518, 731, 1023, 1376
BsmFI	GGGAC	3 cut(s)	121, 772, 1259
BsmI	GAATGC	1 cut(s)	1257
BsnI	GGCC	5 cut(s)	9, 264, 390, 555, 976
Bso31I	GGTCTC	1 cut(s)	731
BsoBI	CYCGRG	1 cut(s)	392
Bsp1286I	GDGCHC	1 cut(s)	77
Bsp143I	GATC	4 cut(s)	343, 645, 806, 1267
Bsp19I	CCATGG	1 cut(s)	259
BspACI	CCGC	3 cut(s)	288, 458, 1040
BspANI	GGCC	5 cut(s)	9, 264, 390, 555, 976
BspCNI	CTCAG	2 cut(s)	1229, 1386
BspHI	TCATGA	1 cut(s)	1225
BspLI	GGNNCC	4 cut(s)	160, 586, 808, 831
BspMAI	CTGCAG	1 cut(s)	694
BspPI	GGATC	3 cut(s)	801, 814, 1262
BspQI	GCTCTTC	1 cut(s)	1179
BspT107I	GGYRCC	1 cut(s)	158
BspTNI	GGTCTC	1 cut(s)	731
BsrFI	RCCGGY	1 cut(s)	832
BsrI	ACTGG	2 cut(s)	245, 760
BssAI	RCCGGY	1 cut(s)	832
BssECI	CCNNGG	4 cut(s)	259, 391, 556, 1179
BssMI	GATC	4 cut(s)	343, 645, 806, 1267
BssT1I	CCWWGG	3 cut(s)	259, 556, 1179
Bst4CI	ACNGT	6 cut(s)	616, 659, 865, 950, 964, 1018
Bst6I	CTCTTC	2 cut(s)	1038, 1179
BstAPI	GCANNNNNTGC	2 cut(s)	773, 1271
BstC8I	GCNNGC	2 cut(s)	50, 286
BstDEI	CTNAG	2 cut(s)	1216, 1373
BstDSI	CCRYGG	1 cut(s)	259
BstF5I	GGATG	4 cut(s)	10, 174, 720, 778
BstKTI	GATC	4 cut(s)	346, 648, 809, 1270
BstMAI	GTCTC	4 cut(s)	518, 731, 1023, 1376
BstMBI	GATC	4 cut(s)	343, 645, 806, 1267
BstMWI	GCNNNNNNNGC	4 cut(s)	479, 577, 773, 1271
BstNSI	RCATGY	4 cut(s)	288, 302, 477, 541
BstSFI	CTRYAG	2 cut(s)	101, 690
BstV1I	GCAGC	3 cut(s)	207, 210, 841
BstX2I	RGATCY	3 cut(s)	343, 806, 1267
BstYI	RGATCY	3 cut(s)	343, 806, 1267
BsuRI	GGCC	5 cut(s)	9, 264, 390, 555, 976
BtgI	CCRYGG	1 cut(s)	259
BtsCI	GGATG	4 cut(s)	10, 174, 720, 778
BtsI	GCAGTG	2 cut(s)	208, 1134
BtsIMutI	CAGTG	4 cut(s)	208, 252, 960, 1134
Cac8I	GCNNGC	2 cut(s)	50, 286
CaiI	CAGNNNCTG	2 cut(s)	796, 1271
CciI	TCATGA	1 cut(s)	1225
Cfr10I	RCCGGY	1 cut(s)	832
Cfr13I	GGNCC	1 cut(s)	835
Csp6I	GTAC	2 cut(s)	41, 159
CviQI	GTAC	2 cut(s)	41, 159
DdeI	CTNAG	2 cut(s)	1216, 1373
DpnI	GATC	4 cut(s)	345, 647, 808, 1269
DpnII	GATC	4 cut(s)	343, 645, 806, 1267
EaeI	YGGCCR	2 cut(s)	7, 262
Eam1104I	CTCTTC	2 cut(s)	1038, 1179
EarI	CTCTTC	2 cut(s)	1038, 1179
Eco130I	CCWWGG	3 cut(s)	259, 556, 1179
Eco147I	AGGCCT	1 cut(s)	390
Eco24I	GRGCYC	1 cut(s)	77
Eco31I	GGTCTC	1 cut(s)	731
Eco47I	GGWCC	1 cut(s)	835
Eco88I	CYCGRG	1 cut(s)	392
EcoRI	GAATTC	1 cut(s)	1048
EcoT14I	CCWWGG	3 cut(s)	259, 556, 1179
EcoT38I	GRGCYC	1 cut(s)	77
ErhI	CCWWGG	3 cut(s)	259, 556, 1179
FaqI	GGGAC	3 cut(s)	121, 772, 1259
Fnu4HI	GCNGC	3 cut(s)	196, 199, 855
FokI	GGATG	4 cut(s)	17, 181, 727, 785
FriOI	GRGCYC	1 cut(s)	77
Fsp4HI	GCNGC	3 cut(s)	196, 199, 855
FspBI	CTAG	8 cut(s)	38, 53, 140, 188, 311, 675, 747, 1256
GluI	GCNGC	3 cut(s)	196, 199, 855
GsuI	CTGGAG	2 cut(s)	144, 342
HaeIII	GGCC	5 cut(s)	9, 264, 390, 555, 976
HapII	CCGG	2 cut(s)	409, 833
HincII	GTYRAC	1 cut(s)	655
HindII	GTYRAC	1 cut(s)	655
HindIII	AAGCTT	1 cut(s)	890
HinfI	GANTC	7 cut(s)	60, 412, 446, 793, 1059, 1259, 1348
HpaII	CCGG	2 cut(s)	409, 833
HphI	GGTGA	2 cut(s)	350, 1054
Hpy166II	GTNNAC	2 cut(s)	185, 655
Hpy188I	TCNGA	2 cut(s)	645, 929
Hpy188III	TCNNGA	6 cut(s)	78, 419, 608, 827, 1226, 1352
Hpy8I	GTNNAC	2 cut(s)	185, 655
HpyAV	CCTTC	4 cut(s)	200, 271, 715, 1310
HpyCH4III	ACNGT	6 cut(s)	616, 659, 865, 950, 964, 1018
HpyCH4IV	ACGT	1 cut(s)	548
HpyCH4V	TGCA	3 cut(s)	692, 767, 1210
HpyF10VI	GCNNNNNNNGC	4 cut(s)	479, 577, 773, 1271
HpyF3I	CTNAG	2 cut(s)	1216, 1373
HpySE526I	ACGT	1 cut(s)	548
KpnI	GGTACC	1 cut(s)	162
Kzo9I	GATC	4 cut(s)	343, 645, 806, 1267
LguI	GCTCTTC	1 cut(s)	1179
LmnI	GCTCC	1 cut(s)	829
Lsp1109I	GCAGC	3 cut(s)	207, 210, 841
LweI	GCATC	3 cut(s)	733, 754, 763
MaeI	CTAG	8 cut(s)	38, 53, 140, 188, 311, 675, 747, 1256
MaeII	ACGT	1 cut(s)	548
MaeIII	GTNAC	4 cut(s)	313, 368, 800, 1060
MalI	GATC	4 cut(s)	345, 647, 808, 1269
MboI	GATC	4 cut(s)	343, 645, 806, 1267
MboII	GAAGA	9 cut(s)	353, 443, 924, 1016, 1055, 1103, 1109, 1196, 1351
MfeI	CAATTG	2 cut(s)	178, 1092
MflI	RGATCY	3 cut(s)	343, 806, 1267
MhlI	GDGCHC	1 cut(s)	77
MlsI	TGGCCA	2 cut(s)	9, 264
MluCI	AATT	7 cut(s)	31, 178, 623, 821, 1035, 1048, 1092
MluNI	TGGCCA	2 cut(s)	9, 264
MlyI	GAGTC	4 cut(s)	421, 440, 1068, 1268
MmeI	TCCRAC	2 cut(s)	263, 1269
MnlI	CCTC	8 cut(s)	78, 119, 401, 610, 902, 982, 987, 1018
Mox20I	TGGCCA	2 cut(s)	9, 264
MscI	TGGCCA	2 cut(s)	9, 264
MseI	TTAA	3 cut(s)	117, 438, 1323
MslI	CAYNNNNRTG	3 cut(s)	272, 494, 675
Msp20I	TGGCCA	2 cut(s)	9, 264
MspA1I	CMGCKG	1 cut(s)	494
MspI	CCGG	2 cut(s)	409, 833
MunI	CAATTG	2 cut(s)	178, 1092
Mva1269I	GAATGC	1 cut(s)	1257
MwoI	GCNNNNNNNGC	4 cut(s)	479, 577, 773, 1271
NcoI	CCATGG	1 cut(s)	259
NdeII	GATC	4 cut(s)	343, 645, 806, 1267
NlaIV	GGNNCC	4 cut(s)	160, 586, 808, 831
NmuCI	GTSAC	2 cut(s)	313, 1060
NspI	RCATGY	4 cut(s)	288, 302, 477, 541
OliI	CACNNNNGTG	2 cut(s)	494, 675
PaeI	GCATGC	1 cut(s)	288
PagI	TCATGA	1 cut(s)	1225
PceI	AGGCCT	1 cut(s)	390
PciSI	GCTCTTC	1 cut(s)	1179
PctI	GAATGC	1 cut(s)	1257
PfeI	GAWTC	3 cut(s)	60, 793, 1348
PkrI	GCNGC	3 cut(s)	197, 200, 856
PleI	GAGTC	4 cut(s)	420, 440, 1067, 1267
PpsI	GAGTC	4 cut(s)	420, 440, 1067, 1267
PspN4I	GGNNCC	4 cut(s)	160, 586, 808, 831
PspPI	GGNCC	1 cut(s)	835
PstI	CTGCAG	1 cut(s)	694
PstNI	CAGNNNCTG	2 cut(s)	796, 1271
PsuI	RGATCY	3 cut(s)	343, 806, 1267
PvuII	CAGCTG	1 cut(s)	494
RsaI	GTAC	2 cut(s)	42, 160
RsaNI	GTAC	2 cut(s)	41, 159
RseI	CAYNNNNRTG	3 cut(s)	272, 494, 675
SapI	GCTCTTC	1 cut(s)	1179
SaqAI	TTAA	3 cut(s)	117, 438, 1323
SatI	GCNGC	3 cut(s)	196, 199, 855
Sau3AI	GATC	4 cut(s)	343, 645, 806, 1267
Sau96I	GGNCC	1 cut(s)	835
SchI	GAGTC	4 cut(s)	421, 440, 1068, 1268
SduI	GDGCHC	1 cut(s)	77
SfaNI	GCATC	3 cut(s)	733, 754, 763
SfcI	CTRYAG	2 cut(s)	101, 690
SinI	GGWCC	1 cut(s)	835
SmiMI	CAYNNNNRTG	3 cut(s)	272, 494, 675
SmlI	CTYRAG	1 cut(s)	65
SmoI	CTYRAG	1 cut(s)	65
SpeI	ACTAGT	1 cut(s)	674
SphI	GCATGC	1 cut(s)	288
Sse9I	AATT	7 cut(s)	31, 178, 623, 821, 1035, 1048, 1092
SseBI	AGGCCT	1 cut(s)	390
SsiI	CCGC	3 cut(s)	288, 458, 1040
SspI	AATATT	1 cut(s)	1167
SspMI	CTAG	8 cut(s)	38, 53, 140, 188, 311, 675, 747, 1256
StuI	AGGCCT	1 cut(s)	390
StyI	CCWWGG	3 cut(s)	259, 556, 1179
TaaI	ACNGT	6 cut(s)	616, 659, 865, 950, 964, 1018
TaiI	ACGT	1 cut(s)	551
TaqI	TCGA	1 cut(s)	945
TaqII	GACCGA	1 cut(s)	719
TasI	AATT	7 cut(s)	31, 178, 623, 821, 1035, 1048, 1092
TfiI	GAWTC	3 cut(s)	60, 793, 1348
Tru1I	TTAA	3 cut(s)	117, 438, 1323
Tru9I	TTAA	3 cut(s)	117, 438, 1323
TscAI	CASTG	4 cut(s)	208, 252, 967, 1134
TseFI	GTSAC	2 cut(s)	313, 1060
TseI	GCWGC	3 cut(s)	195, 198, 854
Tsp45I	GTSAC	2 cut(s)	313, 1060
TspDTI	ATGAA	4 cut(s)	480, 675, 1145, 1214
TspGWI	ACGGA	1 cut(s)	819
TspRI	CASTG	4 cut(s)	208, 252, 967, 1134
VpaK11BI	GGWCC	1 cut(s)	835
XapI	RAATTY	3 cut(s)	31, 821, 1048
XceI	RCATGY	4 cut(s)	288, 302, 477, 541
XcmI	CCANNNNNNNNNTGG	2 cut(s)	246, 845
XspI	CTAG	8 cut(s)	38, 53, 140, 188, 311, 675, 747, 1256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.