Rorug06G0477600

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009328
Physical Location & Seq
Forward (+)
59926773 .. 59929464
2692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0477600.1

Sequence Viewer

Length: 2403 bp
ATGGATCCTCTGTTTCTCATTCTACTTCTCTCTCTGGCCATCACTCCTCCAACTTCATCTTCAATCTCCGACGCATTGAGTGAAGGGTCATCCCTCTCTGTAGAGAAACCAGAAGATGTTCTGATTTCACCGGGTGGTGTTTTCACCGCCGGCTTTCATCAAGTTGGCAACAACTCATATTGCTTTGCCATTTGGTTCAACGAGCCATCATCATCGTCATCACCAGTCTCCTACCAGAACCGAACTATAGTCTGGATGGCAAATCGTGACCAACCAGTCAACGGGAAGCGCTCAAAGCTCTCCCTCCTCAAAACTGGTAATCTCATCTTAACTGATGCTGCTCAATCCCATGTTTGGACTACAACCACCACCTCAATCTCCCCTGCCTGTTTAAGCCTCCACGACTCCGGTAATCTGGTTCTGCTTAATCTGAAGACGAAGGTTTTTCTATGGCAGAGCTTTGATTCCCCAACTGATACCCTTCTTCCACTCCAACCATTCACCAGAATTACCACACTTGTCTCCTCCAGAAGCCAGAGCAACTTCTCCTCTGGCTTCTATAAGCTCTTCTTCGACAATGATAACCTTCTCCGACTACTCTTTGACAGCCCTGAGATTTCTAGTGTCTACTGGCCCAACCCTGCATATGTGAGCTGGGATAATGCAAGGTCTACTTTCAACAGCAGTAGAACTGCCGTGCTTGATTCCTTCGGCAGTTTTGCCTCTTCGGACAATCTGACTTTTCTTGCAGCTGACTATGGTGCAAAGTTGCAGAGAAGATTGACTATTGATTTTGATGGAAATGTTAGATTGTATAGCCGAAAAATGCCAGGAGAGAGTTGGGTGGTTTCATGGCAGGCCTTTTCAGACCCTTGTGTGATTCATGGAATTTGTGGAGCAAACAGCATCTGTAGCTATGATCCCAGTTCTGGTAGGAAATGCTCTTGCCTTCCAGGATATCAGATGGAAAATCATACTGATTGGTCTTTGGGGTGTCAACCGGAATTTCAAGTCTCTTATGGAGAAGGTGAGTCTCGTTTTCTCCAACTTGCCCAAGTTGAGTTCTATGGTTATGATTATAATGAGTACCAAAATTATAGCTATGGTGATTGTGAGAAAAGATGCTTGGAACTGGATAACTGCAAGGGGTTTCAGTACACGTTTAATTCTGGTGTTTATAAGTGTAATCCCAAGACGCAGTTGCGAAGTGGGCACAGGTGGAACACTCAAGGTTACTTCTATCTGAGACTGCCCAAGGCTCACCTCTTCTCTAACACAGACATAAAGCCCCTGAAGAACCTTGGTTTTAGTTGCACAGAAGAAGTTGTTGATCTCGAGAGGGAGTATGTTAAAGATCGAGTCAGTAAGCCGGTGAAATTCCTGCTCTGGTTTGCCTATGGAGTTGGGGGAGTTGAAATCATTTGTATCATTTTGGTTTGGGGTTTATTGAGCAGTACATTCTCAAAATCAAATGAAGATATTCACGGCTACCTTCTTACTGCAACCAGGTTCAAAAGATTTAGCTACGCTGAGCTCAAGACTGCAACGCGGGGTTTTAGTGAAGAAATTGGGAGAGGAGCAGGAGGAGTTGTTTACAAAGGCATATTGGCTGATCAGCGAGTGGCAGCGGTTAAGCTGCTCGGTGAAGCTAACCAAGCAGAAGCAGAATTTCTAGCAGAAGCTAGTACTATTGGGAAGGTGAATCATATGAACTTGATCGAGATGTGGGGATATTGCTCAGAGGGAAAGCACAGGCTTCTTGTTTATGAGTACATGGAGCATGGTTCCTTGGCACAGAAGTTAGCTTCCCATGAGCTTGATTGGGAGAAGAGGTTTGAGATTGCTGTCTGCACTGCAAAGGGTCTAGCTTATTTGCACGAAGAGTGCTTGGAGTGGGTTCTACATTGTGATATAAAGCCTCAAAACATACTGTTGGACTCCAATTTTCAACCAAAGGTTGCAGATTTTGGGCTCTCCAAGATTCTTAACAGAGATGAGCTCAGGAATTCAAGCTTTTCGAGGATACGAGGAACCAGAGGTTACATTGCTCCGGAGTGGGTGTATAATCTTCCCATCACATCCAAAGTGGATGTATACAGCTATGGAGTGGTTGTATTGGAGATGGTGACCGGAAAGAACCCGACAATGGATGTGGAAATCAGTGATGGTGAGCAGAGAAGACTAATTATGTGGGTGAGGGAGAAACTAAATGGAACTGAAATTGCGTCCAGAATCGGAGAGATCATAGATCCCTCATTTGAAGGCAACTATGATGTGGAAAAGATGGAAATTTTGTTAACAGTAGCTTTACATTGTGTTGAAGAAGACAAAGATTCAAGACCAACCATGAGTCAAGTCGTTGAGATGCTTCTACACCACGGCAAAGAATCTCAGTGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

800

Amino Acids

90.2

Weight (kDa)

5.63

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 82 - 166 6.8e-24 D-mannose binding lectin
S_locus_glycop PF00954 244 - 323 3.3e-08 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 519 - 790 8.1e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 520 - 788 4.5e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1080, 1179
AasI GACNNNNNNGTC 1 cut(s) 275
AccI GTMKAC 3 cut(s) 627, 671, 2094
AccII CGCG 1 cut(s) 1549
AccIII TCCGGA 1 cut(s) 2050
AciI CCGC 3 cut(s) 147, 1549, 1628
AclWI GGATC 3 cut(s) 12, 914, 2243
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 6 cut(s) 888, 1004, 1376, 1667, 2005, 2289
AcuI CTGAAG 2 cut(s) 452, 1313
AdeI CACNNNGTG 1 cut(s) 134
AfaI GTAC 5 cut(s) 1088, 1157, 1456, 1687, 1772
AfeI AGCGCT 1 cut(s) 290
AfiI CCNNNNNNNGG 4 cut(s) 281, 354, 929, 2146
AflIII ACRYGT 1 cut(s) 1158
AjnI CCWGG 3 cut(s) 829, 952, 1505
AjuI GAANNNNNNNTTGG 4 cut(s) 43, 75, 1109, 1141
Alw21I GWGCWC 2 cut(s) 1536, 2001
Alw26I GTCTC 5 cut(s) 232, 526, 1018, 1038, 1240
AlwI GGATC 3 cut(s) 12, 914, 2243
AlwNI CAGNNNCTG 1 cut(s) 909
Ama87I CYCGRG 1 cut(s) 1334
Aor13HI TCCGGA 1 cut(s) 2050
Aor51HI AGCGCT 1 cut(s) 290
AoxI GGCC 3 cut(s) 36, 632, 858
ApeKI GCWGC 4 cut(s) 338, 749, 1625, 1636
ApoI RAATTY 6 cut(s) 888, 1004, 1376, 1667, 2005, 2289
Asp700I GAANNNNTTC 2 cut(s) 117, 1479
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 633
AsuC2I CCSGG 1 cut(s) 132
AvaI CYCGRG 1 cut(s) 1334
BaeGI GKGCMC 1 cut(s) 1215
BalI TGGCCA 1 cut(s) 38
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 1536, 1974, 2001
BbsI GAAGAC 3 cut(s) 440, 2185, 2331
Bbv12I GWGCWC 2 cut(s) 1536, 2001
BbvI GCAGC 4 cut(s) 325, 761, 1623, 1637
BccI CCATC 9 cut(s) 47, 214, 250, 791, 958, 2081, 2116, 2159, 2278
BceAI ACGGC 3 cut(s) 680, 1501, 2395
BciT130I CCWGG 3 cut(s) 831, 954, 1507
BciVI GTATCC 1 cut(s) 2016
BclI TGATCA 1 cut(s) 1612
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 5 cut(s) 232, 526, 1018, 1038, 1240
BfaI CTAG 4 cut(s) 621, 1673, 1683, 1865
BfmI CTRYAG 3 cut(s) 99, 246, 910
BfoI RGCGCY 1 cut(s) 292
BfuI GTATCC 1 cut(s) 2016
BisI GCNGC 4 cut(s) 339, 750, 1626, 1637
BlpI GCTNAGC 1 cut(s) 1530
BlsI GCNGC 4 cut(s) 340, 751, 1627, 1638
BmcAI AGTACT 1 cut(s) 1687
Bme1390I CCNGG 4 cut(s) 132, 831, 954, 1507
BmeT110I CYCGRG 1 cut(s) 1334
BmgT120I GGNCC 1 cut(s) 633
BmiI GGNNCC 3 cut(s) 6, 1786, 2032
BmrFI CCNGG 4 cut(s) 132, 831, 954, 1507
BmrI ACTGGG 1 cut(s) 918
BmsI GCATC 4 cut(s) 325, 915, 1112, 2355
BmuI ACTGGG 1 cut(s) 918
BpiI GAAGAC 3 cut(s) 440, 2185, 2331
BplI GAGNNNNNCTC 2 cut(s) 1983, 2015
BpmI CTGGAG 1 cut(s) 511
Bpu10I CCTNAGC 1 cut(s) 2000
Bpu1102I GCTNAGC 1 cut(s) 1530
BpuEI CTTGAG 2 cut(s) 1212, 1520
BpuMI CCSGG 1 cut(s) 132
BsaJI CCNNGG 4 cut(s) 1254, 1300, 1788, 2377
BsaWI WCCGGW 4 cut(s) 407, 1000, 2050, 2129
BsaXI ACNNNNNCTCC 8 cut(s) 1575, 1605, 1817, 1847, 2045, 2075, 2097, 2127
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 354, 929, 2146
Bse118I RCCGGY 2 cut(s) 149, 1369
Bse1I ACTGG 6 cut(s) 224, 275, 319, 635, 924, 1137
Bse3DI GCAATG 1 cut(s) 2043
BseAI TCCGGA 1 cut(s) 2050
BseBI CCWGG 3 cut(s) 831, 954, 1507
BseDI CCNNGG 4 cut(s) 1254, 1300, 1788, 2377
BseGI GGATG 5 cut(s) 89, 261, 2078, 2095, 2155
BseLI CCNNNNNNNGG 4 cut(s) 281, 354, 929, 2146
BseMI GCAATG 1 cut(s) 2043
BseMII CTCAG 5 cut(s) 603, 1235, 1521, 1752, 2014
BseNI ACTGG 6 cut(s) 224, 275, 319, 635, 924, 1137
BseRI GAGGAG 6 cut(s) 36, 296, 514, 538, 1590, 1599
BseSI GKGCMC 1 cut(s) 1215
BseXI GCAGC 4 cut(s) 325, 761, 1623, 1637
BseYI CCCAGC 1 cut(s) 654
BsgI GTGCAG 1 cut(s) 1834
Bsh1236I CGCG 1 cut(s) 1549
BshFI GGCC 3 cut(s) 38, 634, 860
BsiHKAI GWGCWC 2 cut(s) 1536, 2001
BsiHKCI CYCGRG 1 cut(s) 1334
BsiSI CCGG 7 cut(s) 131, 150, 408, 1001, 1370, 2051, 2130
BslI CCNNNNNNNGG 4 cut(s) 281, 354, 929, 2146
BsmAI GTCTC 5 cut(s) 232, 526, 1018, 1038, 1240
BsnI GGCC 3 cut(s) 38, 634, 860
BsoBI CYCGRG 1 cut(s) 1334
Bsp1286I GDGCHC 4 cut(s) 1215, 1536, 1974, 2001
Bsp13I TCCGGA 1 cut(s) 2050
Bsp143I GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
Bsp1720I GCTNAGC 1 cut(s) 1530
BspACI CCGC 3 cut(s) 147, 1549, 1628
BspANI GGCC 3 cut(s) 38, 634, 860
BspCNI CTCAG 5 cut(s) 604, 1236, 1522, 1751, 2013
BspEI TCCGGA 1 cut(s) 2050
BspFNI CGCG 1 cut(s) 1549
BspLI GGNNCC 3 cut(s) 6, 1786, 2032
BspPI GGATC 3 cut(s) 12, 914, 2243
BspQI GCTCTTC 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 2043
BsrFI RCCGGY 2 cut(s) 149, 1369
BsrI ACTGG 6 cut(s) 224, 275, 319, 635, 924, 1137
BssAI RCCGGY 2 cut(s) 149, 1369
BssECI CCNNGG 4 cut(s) 1254, 1300, 1788, 2377
BssMI GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
BssNAI GTATAC 1 cut(s) 2095
BssT1I CCWWGG 3 cut(s) 1254, 1300, 1788
Bst1107I GTATAC 1 cut(s) 2095
Bst2UI CCWGG 3 cut(s) 831, 954, 1507
Bst4CI ACNGT 2 cut(s) 1932, 2302
Bst6I CTCTTC 5 cut(s) 572, 730, 1271, 1823, 1875
BstC8I GCNNGC 2 cut(s) 151, 858
BstDEI CTNAG 6 cut(s) 612, 1244, 1530, 1738, 2000, 2391
BstDSI CCRYGG 1 cut(s) 2377
BstEII GGTNACC 1 cut(s) 2125
BstF5I GGATG 5 cut(s) 89, 261, 2078, 2095, 2155
BstFNI CGCG 1 cut(s) 1549
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 8 cut(s) 7, 922, 1333, 1357, 1615, 1719, 2244, 2251
BstMAI GTCTC 5 cut(s) 232, 526, 1018, 1038, 1240
BstMBI GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
BstMWI GCNNNNNNNGC 4 cut(s) 295, 912, 1210, 1655
BstNI CCWGG 3 cut(s) 831, 954, 1507
BstPI GGTNACC 1 cut(s) 2125
BstSCI CCNGG 4 cut(s) 130, 829, 952, 1505
BstSFI CTRYAG 3 cut(s) 99, 246, 910
BstSLI GKGCMC 1 cut(s) 1215
BstUI CGCG 1 cut(s) 1549
BstV1I GCAGC 4 cut(s) 325, 761, 1623, 1637
BstV2I GAAGAC 3 cut(s) 440, 2185, 2331
BstX2I RGATCY 2 cut(s) 4, 2248
BstYI RGATCY 2 cut(s) 4, 2248
BstZ17I GTATAC 1 cut(s) 2095
BsuI GTATCC 1 cut(s) 2016
BsuRI GGCC 3 cut(s) 38, 634, 860
BtgI CCRYGG 1 cut(s) 2377
BtsCI GGATG 5 cut(s) 89, 261, 2078, 2095, 2155
BtsI GCAGTG 1 cut(s) 1851
BtsIMutI CAGTG 3 cut(s) 1851, 2167, 2399
Cac8I GCNNGC 2 cut(s) 151, 858
CaiI CAGNNNCTG 1 cut(s) 909
CfoI GCGC 1 cut(s) 291
Cfr10I RCCGGY 2 cut(s) 149, 1369
Cfr13I GGNCC 1 cut(s) 633
CseI GACGC 3 cut(s) 80, 1204, 2214
CsiI ACCWGGT 1 cut(s) 1505
Csp6I GTAC 5 cut(s) 1087, 1156, 1455, 1686, 1771
CspCI CAANNNNNGTGG 2 cut(s) 2133, 2168
CviAII CATG 7 cut(s) 350, 852, 884, 1774, 1781, 1811, 2347
CviQI GTAC 5 cut(s) 1087, 1156, 1455, 1686, 1771
DdeI CTNAG 6 cut(s) 612, 1244, 1530, 1738, 2000, 2391
DpnI GATC 8 cut(s) 6, 921, 1332, 1356, 1614, 1718, 2243, 2250
DpnII GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
DraIII CACNNNGTG 1 cut(s) 134
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 5 cut(s) 572, 730, 1271, 1823, 1875
EarI CTCTTC 5 cut(s) 572, 730, 1271, 1823, 1875
Ecl136II GAGCTC 2 cut(s) 1534, 1999
Eco130I CCWWGG 3 cut(s) 1254, 1300, 1788
Eco147I AGGCCT 1 cut(s) 860
Eco24I GRGCYC 3 cut(s) 1536, 1974, 2001
Eco32I GATATC 1 cut(s) 959
Eco47III AGCGCT 1 cut(s) 290
Eco53kI GAGCTC 2 cut(s) 1534, 1999
Eco57I CTGAAG 2 cut(s) 452, 1313
Eco88I CYCGRG 1 cut(s) 1334
Eco91I GGTNACC 1 cut(s) 2125
EcoICRI GAGCTC 2 cut(s) 1534, 1999
EcoO65I GGTNACC 1 cut(s) 2125
EcoRI GAATTC 1 cut(s) 2005
EcoRII CCWGG 3 cut(s) 829, 952, 1505
EcoRV GATATC 1 cut(s) 959
EcoT14I CCWWGG 3 cut(s) 1254, 1300, 1788
EcoT38I GRGCYC 3 cut(s) 1536, 1974, 2001
ErhI CCWWGG 3 cut(s) 1254, 1300, 1788
FaeI CATG 7 cut(s) 353, 855, 887, 1777, 1784, 1814, 2350
FalI AAGNNNNNCTT 4 cut(s) 554, 586, 658, 690
FatI CATG 7 cut(s) 349, 851, 883, 1773, 1780, 1810, 2346
FauI CCCGC 1 cut(s) 1542
FauNDI CATATG 2 cut(s) 646, 1707
FbaI TGATCA 1 cut(s) 1612
FblI GTMKAC 3 cut(s) 627, 671, 2094
Fnu4HI GCNGC 4 cut(s) 339, 750, 1626, 1637
FokI GGATG 5 cut(s) 76, 268, 2065, 2102, 2162
FriOI GRGCYC 3 cut(s) 1536, 1974, 2001
Fsp4HI GCNGC 4 cut(s) 339, 750, 1626, 1637
FspBI CTAG 4 cut(s) 621, 1673, 1683, 1865
GlaI GCGC 1 cut(s) 290
GluI GCNGC 4 cut(s) 339, 750, 1626, 1637
GsaI CCCAGC 1 cut(s) 658
GsuI CTGGAG 1 cut(s) 511
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 3 cut(s) 38, 634, 860
HapII CCGG 7 cut(s) 131, 150, 408, 1001, 1370, 2051, 2130
HgaI GACGC 3 cut(s) 80, 1204, 2214
HhaI GCGC 1 cut(s) 291
Hin1II CATG 7 cut(s) 353, 855, 887, 1777, 1784, 1814, 2350
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 3 cut(s) 280, 998, 2298
HindII GTYRAC 3 cut(s) 280, 998, 2298
HindIII AAGCTT 1 cut(s) 2011
HpaI GTTAAC 1 cut(s) 2298
HpaII CCGG 7 cut(s) 131, 150, 408, 1001, 1370, 2051, 2130
Hpy166II GTNNAC 8 cut(s) 280, 628, 672, 998, 1158, 1594, 2095, 2298
Hpy8I GTNNAC 8 cut(s) 280, 628, 672, 998, 1158, 1594, 2095, 2298
Hpy99I CGWCG 1 cut(s) 74
HpyCH4III ACNGT 2 cut(s) 1932, 2302
HpyCH4IV ACGT 1 cut(s) 1160
HpyF10VI GCNNNNNNNGC 4 cut(s) 295, 912, 1210, 1655
HpyF3I CTNAG 6 cut(s) 612, 1244, 1530, 1738, 2000, 2391
HpySE526I ACGT 1 cut(s) 1160
Hsp92II CATG 7 cut(s) 353, 855, 887, 1777, 1784, 1814, 2350
HspAI GCGC 1 cut(s) 289
Kpn2I TCCGGA 1 cut(s) 2050
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 1612
KspAI GTTAAC 1 cut(s) 2298
Kzo9I GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
LguI GCTCTTC 1 cut(s) 572
LmnI GCTCC 4 cut(s) 896, 1577, 1777, 2053
Lsp1109I GCAGC 4 cut(s) 325, 761, 1623, 1637
LweI GCATC 4 cut(s) 325, 915, 1112, 2355
MabI ACCWGGT 1 cut(s) 1505
MaeI CTAG 4 cut(s) 621, 1673, 1683, 1865
MaeII ACGT 1 cut(s) 1160
MaeIII GTNAC 4 cut(s) 266, 1232, 2039, 2125
MalI GATC 8 cut(s) 6, 921, 1332, 1356, 1614, 1718, 2243, 2250
MboI GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
MflI RGATCY 2 cut(s) 4, 2248
MhlI GDGCHC 4 cut(s) 1215, 1536, 1974, 2001
MlsI TGGCCA 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 5 cut(s) 398, 1040, 1368, 1931, 2359
MmeI TCCRAC 6 cut(s) 74, 93, 517, 616, 1069, 1914
Mox20I TGGCCA 1 cut(s) 38
MroI TCCGGA 1 cut(s) 2050
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 2 cut(s) 117, 1479
MscI TGGCCA 1 cut(s) 38
MseI TTAA 8 cut(s) 329, 392, 426, 1164, 1350, 1632, 1986, 2297
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 2 cut(s) 752, 1628
MspI CCGG 7 cut(s) 131, 150, 408, 1001, 1370, 2051, 2130
MspR9I CCNGG 4 cut(s) 132, 831, 954, 1507
MvaI CCWGG 3 cut(s) 831, 954, 1507
MvnI CGCG 1 cut(s) 1549
MwoI GCNNNNNNNGC 4 cut(s) 295, 912, 1210, 1655
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 132
NdeI CATATG 2 cut(s) 646, 1707
NdeII GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 7 cut(s) 353, 855, 887, 1777, 1784, 1814, 2350
NlaIV GGNNCC 3 cut(s) 6, 1786, 2032
NmuCI GTSAC 2 cut(s) 266, 2125
PaeR7I CTCGAG 1 cut(s) 1334
PceI AGGCCT 1 cut(s) 860
PciSI GCTCTTC 1 cut(s) 572
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 117, 1479
PfeI GAWTC 8 cut(s) 464, 704, 880, 1702, 1981, 2232, 2333, 2387
PfoI TCCNGGA 1 cut(s) 952
PkrI GCNGC 4 cut(s) 340, 751, 1627, 1638
PleI GAGTC 5 cut(s) 398, 1039, 1367, 1931, 2358
PpsI GAGTC 5 cut(s) 398, 1039, 1367, 1931, 2358
PsiI TTATAA 2 cut(s) 1080, 1179
Psp124BI GAGCTC 2 cut(s) 1536, 2001
Psp6I CCWGG 3 cut(s) 829, 952, 1505
PspEI GGTNACC 1 cut(s) 2125
PspFI CCCAGC 1 cut(s) 654
PspGI CCWGG 3 cut(s) 829, 952, 1505
PspN4I GGNNCC 3 cut(s) 6, 1786, 2032
PspPI GGNCC 1 cut(s) 633
PstNI CAGNNNCTG 1 cut(s) 909
PsuI RGATCY 2 cut(s) 4, 2248
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 5 cut(s) 1088, 1157, 1456, 1687, 1772
RsaNI GTAC 5 cut(s) 1087, 1156, 1455, 1686, 1771
SacI GAGCTC 2 cut(s) 1536, 2001
SapI GCTCTTC 1 cut(s) 572
SaqAI TTAA 8 cut(s) 329, 392, 426, 1164, 1350, 1632, 1986, 2297
SatI GCNGC 4 cut(s) 339, 750, 1626, 1637
Sau3AI GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
Sau96I GGNCC 1 cut(s) 633
ScaI AGTACT 1 cut(s) 1687
SchI GAGTC 5 cut(s) 398, 1040, 1368, 1931, 2359
ScrFI CCNGG 4 cut(s) 132, 831, 954, 1507
SduI GDGCHC 4 cut(s) 1215, 1536, 1974, 2001
SexAI ACCWGGT 1 cut(s) 1505
SfaNI GCATC 4 cut(s) 325, 915, 1112, 2355
SfcI CTRYAG 3 cut(s) 99, 246, 910
Sfr274I CTCGAG 1 cut(s) 1334
SlaI CTCGAG 1 cut(s) 1334
SmlI CTYRAG 3 cut(s) 1227, 1334, 1535
SmoI CTYRAG 3 cut(s) 1227, 1334, 1535
SseBI AGGCCT 1 cut(s) 860
SsiI CCGC 3 cut(s) 147, 1549, 1628
SspMI CTAG 4 cut(s) 621, 1673, 1683, 1865
SstI GAGCTC 2 cut(s) 1536, 2001
StuI AGGCCT 1 cut(s) 860
StyD4I CCNGG 4 cut(s) 130, 829, 952, 1505
StyI CCWWGG 3 cut(s) 1254, 1300, 1788
TaaI ACNGT 2 cut(s) 1932, 2302
TaiI ACGT 1 cut(s) 1163
TaqI TCGA 5 cut(s) 573, 1335, 1357, 1719, 2018
TatI WGTACW 4 cut(s) 1155, 1454, 1685, 1770
TfiI GAWTC 8 cut(s) 464, 704, 880, 1702, 1981, 2232, 2333, 2387
Tru1I TTAA 8 cut(s) 329, 392, 426, 1164, 1350, 1632, 1986, 2297
Tru9I TTAA 8 cut(s) 329, 392, 426, 1164, 1350, 1632, 1986, 2297
TscAI CASTG 3 cut(s) 1858, 2167, 2399
TseFI GTSAC 2 cut(s) 266, 2125
TseI GCWGC 4 cut(s) 338, 749, 1625, 1636
Tsp45I GTSAC 2 cut(s) 266, 2125
TspDTI ATGAA 6 cut(s) 45, 146, 840, 872, 1488, 1724
TspRI CASTG 3 cut(s) 1858, 2167, 2399
XapI RAATTY 6 cut(s) 888, 1004, 1376, 1667, 2005, 2289
XcmI CCANNNNNNNNNTGG 1 cut(s) 837
XhoI CTCGAG 1 cut(s) 1334
XmiI GTMKAC 3 cut(s) 627, 671, 2094
XmnI GAANNNNTTC 2 cut(s) 117, 1479
XspI CTAG 4 cut(s) 621, 1673, 1683, 1865
ZrmI AGTACT 1 cut(s) 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.