Rorug06G0477700

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009328
Physical Location & Seq
Forward (+)
59942672 .. 59945110
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0477700.1

Sequence Viewer

Length: 2439 bp
ATGGTGCGATTGAGGAGTTCAACTAAACTCATGGACAGGGTTCTGTTTCTGTGCCTTGTTCTTGTTTTTGCACTAATTTTTCCATTCAAATCATCAACAGCTTTTAATTTGAGCATGGGATCCTTTCTTTCAGTAGAAAATCCATCTCAAACTTTGATGTCTCCGGACGCAGTATTTACCGCTGGCTTTTTTCCGGTCGGTGAAAATGCGTTTTGCTTTGGAATATGGTTCTCGGAGCCATCAACCAGTACTCACAACTTCACCGTGGTGTGGATGGCAAATCGTGACGAGCCGGTCAACGGTCAACGCTCTAAGCTCACCCTGCACAAAAGTGGAAACCTTATTCTCTCCGACATCGACCGATACACCGTGTGGTCCTCCAACACAGCTTCAAACTCATCGGTCCACCTGCACCTTAGAAACACCGGCAATCTTGTTCTGCTCGCTGCGCACGGCGGCGGCGTATTGTGGGAAAGCTTTGCTTCGCCGACGGACACACTTTTGCCTCAGCAAGCGCTCACTAGAAACACACAGCTCATTTCTTCAAGAAGCCTCACCAACTATTCCTCCGGCTTCTACTCTTTTTACTTCGACAACGACAACACTCTTCATATGCTCTACAACGGCCCCGAGGTTTCGAGCATTTACTGGCCTGATCCATGGCGTCTGATTTGGCAAGTAGGAAGATCCACAAACAACAACAGTCGAATCGCAACTTTTGACAGCTTGGGAAATTTTAGTGCATCTGATAATTTTACCGTTTGGACAATCGACTACGGTGCAAATTTGCAGCGAATATTCAGGATGGATGTAGATGGTAATCTCCGAGGTTACAGTCGAAAAGGTCCTGGAGATAAATGGATTGTTACATGGGAGGCAGTTTTACAACCTTGTAAAATTCATGGCTTATGCGGAGCTAATAGCTATTGCAGCTATATTCCTAGTACAGGTACGAAATGCTCATGCCTGCCGGGTTATGAAATGATCAGTAAAACCGATTGGTCTTATGGGTGTAAACCGGCTGGTTTCAATCCGTCCTGCAAGAGTAGTGCACGGGAGGAGTTTGGTTTTGTGCATCTTCCACGTATTGAGATGTATGGTTATGATTATGGCGTCTATGGCAATCACACATTAGATGAGTGCAAAAGTTTGTGCCTGGACTTGTGCAACTGTACTGGATTCCAGCACAAATTTGGCTACAAATTTGGTGGTGGTCAGTATGAGTGTTTTCCCAAGGCACAATTACGAAATGGGCACCGGTCACCTGCTTTTGATGGGGAGCTGTATGTCAAGCTACCAAAGGGCCTCCTCAATGCTACCTATAGAGATACCAACATGTGCTCAGCTAAACTTACAGCACAGCTTGACAGAACATATGAAAGAAGCAAGCCCAATGGATCGATTGAGTTTATGCTGAAATTTGCATCTGCCTTGGGAGGAATAGAGGTTATTTGCATCTTTGTTGTGTGGTTTTTTGTGACAAGAAGAAGCGGTGGAGATAAAAAAGATTCAGAACTAGAACTACTTTCTCAAGGCTACCTTCAAGCTGCCAGTGGATTCAGAAGGTTTACTTACTCCGAGCTGAAGAAGGCCACAAAAGGTTTTAAAGAAGAGATTGGCAGAGGAGGAGGAGGAGTTGTGTACAAAGGCATATTGTCTGATCAACGAGTTGCAGCGATCAAGCAACTGAATGATTGTAAACAAGGGGGTGAAGCAGAGTTCCTTGCAGAAGTGAGCCTTATTGGTAAGCTCTACCATATGAACTTGATAGAGATGTGGGGTTATTGCGTAGAAGACAAGCATAGGCTTCTAGTGTATGAGTACATGGAGCATGGTTCTTTGGCGAAAAATTTATCTTCCAATGTGCTTGATTGGAAGAAGAGGTATGAAATTGCTCTAGGCACTGCGAAAGGCCTGGCTTATCTACATGAAGAGTGCTTGGAATGGGTTTTACACTGTGATGTGAAGCCGCAAAACATACTATTGGACTCCGACTACAATCCAAAGGTAGCTGATTTTGGATTGTCCAAGCTATTAAACAGAGGTGAGAACACCAGCTCGGACTTCTACATGAAGATAAGGGGAACCAGAGGTTACATGGCTCCGGAGTGGGTGCACAATATGCCAATCACTTCCAAAGTGGATGTTTACAGTTACGGGATTGTTGTATTGGAGATGCTGACCGGAAAGAACCCAACTGAAAGCAACGTTCAATCTGTTGAAGGTGGAGGGGAGACACAGAAGCAAATAATGGTCAAATGGGTCATGGAGAAAATGGATGGAGCGTTTACAAATGCAGAGACTGAGATGGGAGGTGAAAATGGAGAGGGTAAGCTGGAAATTCTTGTTAAAGTGGCTCTTCAGTGTATAGAGGAAGACAGAAATGCAAGACCCTCAATGAGTCGAGTAGTTGAGATGCTTCTACATCTTGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

812

Amino Acids

91.12

Weight (kDa)

6.81

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 88 - 186 3.9e-24 D-mannose binding lectin
S_locus_glycop PF00954 260 - 327 7.3e-07 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 535 - 807 3.2e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 536 - 805 1.9e-53 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 417, 1273
AasI GACNNNNNNGTC 1 cut(s) 293
Acc16I TGCGCA 1 cut(s) 450
Acc36I ACCTGC 2 cut(s) 417, 1273
AccB1I GGYRCC 1 cut(s) 1254
AccIII TCCGGA 2 cut(s) 163, 2104
AciI CCGC 6 cut(s) 180, 456, 459, 912, 1491, 1970
AclI AACGTT 1 cut(s) 2208
AclWI GGATC 5 cut(s) 114, 127, 650, 681, 1405
AcsI RAATTY 8 cut(s) 733, 784, 897, 1190, 1202, 1418, 1849, 2340
AcuI CTGAAG 2 cut(s) 1604, 2345
AcyI GRCGYC 2 cut(s) 664, 1113
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 6 cut(s) 250, 946, 952, 1174, 1643, 1823
AfeI AGCGCT 1 cut(s) 516
AfiI CCNNNNNNNGG 3 cut(s) 270, 299, 947
AflIII ACRYGT 1 cut(s) 1335
AgeI ACCGGT 1 cut(s) 1257
AgsI TTSAA 9 cut(s) 21, 88, 393, 546, 1030, 1544, 2213, 2222, 2432
AjnI CCWGG 3 cut(s) 847, 1155, 1914
AleI CACNNNNGTG 2 cut(s) 266, 330
Alw21I GWGCWC 3 cut(s) 1054, 1343, 2118
Alw26I GTCTC 3 cut(s) 165, 2228, 2294
Alw44I GTGCAC 2 cut(s) 1050, 2114
AlwI GGATC 5 cut(s) 114, 127, 650, 681, 1405
AlwNI CAGNNNCTG 1 cut(s) 2303
Ama87I CYCGRG 1 cut(s) 629
Aor13HI TCCGGA 2 cut(s) 163, 2104
Aor51HI AGCGCT 1 cut(s) 516
AoxI GGCC 5 cut(s) 625, 650, 1303, 1590, 1912
ApaLI GTGCAC 2 cut(s) 1050, 2114
ApeKI GCWGC 5 cut(s) 446, 790, 930, 1547, 1673
ApoI RAATTY 8 cut(s) 733, 784, 897, 1190, 1202, 1418, 1849, 2340
AsiGI ACCGGT 1 cut(s) 1257
AspLEI GCGC 2 cut(s) 451, 517
AspS9I GGNCC 5 cut(s) 375, 403, 626, 845, 1303
AsuC2I CCSGG 1 cut(s) 972
AsuHPI GGTGA 8 cut(s) 212, 253, 310, 547, 1254, 1721, 2057, 2327
AvaI CYCGRG 1 cut(s) 629
AvaII GGWCC 3 cut(s) 375, 403, 845
BaeGI GKGCMC 3 cut(s) 1054, 1257, 2118
BamHI GGATCC 1 cut(s) 119
BanI GGYRCC 1 cut(s) 1254
BbsI GAAGAC 2 cut(s) 1800, 2382
Bbv12I GWGCWC 3 cut(s) 1054, 1343, 2118
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 5 cut(s) 433, 802, 942, 1534, 1685
BccI CCATC 8 cut(s) 151, 247, 268, 799, 809, 1268, 2273, 2302
BceAI ACGGC 2 cut(s) 469, 640
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 3 cut(s) 849, 1157, 1916
BclI TGATCA 2 cut(s) 984, 1660
BcnI CCSGG 1 cut(s) 972
BcoDI GTCTC 3 cut(s) 165, 2228, 2294
BfaI CTAG 5 cut(s) 522, 942, 1517, 1811, 1898
BfmI CTRYAG 1 cut(s) 1321
BfoI RGCGCY 1 cut(s) 518
BfuAI ACCTGC 2 cut(s) 417, 1273
BisI GCNGC 8 cut(s) 447, 457, 460, 791, 931, 1548, 1674, 1970
BlpI GCTNAGC 1 cut(s) 1342
BlsI GCNGC 8 cut(s) 448, 458, 461, 792, 932, 1549, 1675, 1971
BmcAI AGTACT 1 cut(s) 250
Bme1390I CCNGG 4 cut(s) 849, 972, 1157, 1916
Bme18I GGWCC 3 cut(s) 375, 403, 845
BmeT110I CYCGRG 1 cut(s) 629
BmgT120I GGNCC 5 cut(s) 375, 403, 626, 845, 1303
BmiI GGNNCC 6 cut(s) 121, 237, 628, 1256, 2086, 2103
BmrFI CCNGG 4 cut(s) 849, 972, 1157, 1916
BmsI GCATC 6 cut(s) 752, 1084, 1433, 1464, 2166, 2406
BpiI GAAGAC 2 cut(s) 1800, 2382
BpmI CTGGAG 1 cut(s) 870
Bpu10I CCTNAGC 1 cut(s) 507
Bpu1102I GCTNAGC 1 cut(s) 1342
BpuEI CTTGAG 1 cut(s) 1515
BpuMI CCSGG 1 cut(s) 972
Bsa29I ATCGAT 1 cut(s) 1400
BsaAI YACGTR 1 cut(s) 1085
BsaBI GATNNNNATC 1 cut(s) 819
BsaHI GRCGYC 2 cut(s) 664, 1113
BsaJI CCNNGG 6 cut(s) 264, 630, 659, 826, 1233, 1431
BsaWI WCCGGW 5 cut(s) 163, 193, 1257, 2104, 2183
BsaXI ACNNNNNCTCC 6 cut(s) 551, 581, 1620, 1623, 1650, 1653
Bsc4I CCNNNNNNNGG 3 cut(s) 270, 299, 947
Bse118I RCCGGY 4 cut(s) 292, 425, 1018, 1257
Bse1I ACTGG 4 cut(s) 246, 653, 1180, 1551
Bse8I GATNNNNATC 1 cut(s) 819
BseAI TCCGGA 2 cut(s) 163, 2104
BseBI CCWGG 3 cut(s) 849, 1157, 1916
BseCI ATCGAT 1 cut(s) 1400
BseDI CCNNGG 6 cut(s) 264, 630, 659, 826, 1233, 1431
BseGI GGATG 5 cut(s) 279, 810, 814, 2149, 2284
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 3 cut(s) 270, 299, 947
BseMII CTCAG 3 cut(s) 521, 1356, 2295
BseNI ACTGG 4 cut(s) 246, 653, 1180, 1551
BseRI GAGGAG 7 cut(s) 28, 1073, 1298, 1638, 1641, 1644, 1647
BseSI GKGCMC 3 cut(s) 1054, 1257, 2118
BseXI GCAGC 5 cut(s) 433, 802, 942, 1534, 1685
BsgI GTGCAG 2 cut(s) 308, 395
Bsh1285I CGRYCG 2 cut(s) 198, 361
BshFI GGCC 5 cut(s) 627, 652, 1305, 1592, 1914
BshNI GGYRCC 1 cut(s) 1254
BshTI ACCGGT 1 cut(s) 1257
BshVI ATCGAT 1 cut(s) 1400
BsiEI CGRYCG 2 cut(s) 198, 361
BsiHKAI GWGCWC 3 cut(s) 1054, 1343, 2118
BsiHKCI CYCGRG 1 cut(s) 629
BslI CCNNNNNNNGG 3 cut(s) 270, 299, 947
BsmAI GTCTC 3 cut(s) 165, 2228, 2294
BsnI GGCC 5 cut(s) 627, 652, 1305, 1592, 1914
BsoBI CYCGRG 1 cut(s) 629
Bsp1286I GDGCHC 4 cut(s) 1054, 1257, 1343, 2118
Bsp13I TCCGGA 2 cut(s) 163, 2104
Bsp1407I TGTACA 1 cut(s) 1641
Bsp143I GATC 7 cut(s) 119, 655, 686, 984, 1397, 1660, 1677
Bsp1720I GCTNAGC 1 cut(s) 1342
Bsp19I CCATGG 1 cut(s) 659
BspACI CCGC 6 cut(s) 180, 456, 459, 912, 1491, 1970
BspANI GGCC 5 cut(s) 627, 652, 1305, 1592, 1914
BspCNI CTCAG 3 cut(s) 520, 1355, 2296
BspDI ATCGAT 1 cut(s) 1400
BspEI TCCGGA 2 cut(s) 163, 2104
BspLI GGNNCC 6 cut(s) 121, 237, 628, 1256, 2086, 2103
BspMI ACCTGC 2 cut(s) 417, 1273
BspPI GGATC 5 cut(s) 114, 127, 650, 681, 1405
BspQI GCTCTTC 1 cut(s) 2364
BspT107I GGYRCC 1 cut(s) 1254
BsrFI RCCGGY 4 cut(s) 292, 425, 1018, 1257
BsrGI TGTACA 1 cut(s) 1641
BsrI ACTGG 4 cut(s) 246, 653, 1180, 1551
BssAI RCCGGY 4 cut(s) 292, 425, 1018, 1257
BssECI CCNNGG 6 cut(s) 264, 630, 659, 826, 1233, 1431
BssMI GATC 7 cut(s) 119, 655, 686, 984, 1397, 1660, 1677
BssNI GRCGYC 2 cut(s) 664, 1113
BssT1I CCWWGG 3 cut(s) 659, 1233, 1431
Bst2UI CCWGG 3 cut(s) 849, 1157, 1916
Bst6I CTCTTC 5 cut(s) 612, 1605, 1874, 1926, 2364
BstACI GRCGYC 2 cut(s) 664, 1113
BstAPI GCANNNNNTGC 1 cut(s) 2122
BstAUI TGTACA 1 cut(s) 1641
BstBAI YACGTR 1 cut(s) 1085
BstC8I GCNNGC 5 cut(s) 184, 444, 513, 968, 1388
BstDEI CTNAG 5 cut(s) 312, 416, 507, 1342, 2304
BstDSI CCRYGG 2 cut(s) 264, 659
BstEII GGTNACC 1 cut(s) 1260
BstENI CCTNNNNNAGG 1 cut(s) 945
BstF5I GGATG 5 cut(s) 279, 810, 814, 2149, 2284
BstH2I RGCGCY 1 cut(s) 518
BstHHI GCGC 2 cut(s) 451, 517
BstKTI GATC 7 cut(s) 122, 658, 689, 987, 1400, 1663, 1680
BstMAI GTCTC 3 cut(s) 165, 2228, 2294
BstMBI GATC 7 cut(s) 119, 655, 686, 984, 1397, 1660, 1677
BstMCI CGRYCG 2 cut(s) 198, 361
BstMWI GCNNNNNNNGC 5 cut(s) 322, 448, 930, 1119, 2122
BstNI CCWGG 3 cut(s) 849, 1157, 1916
BstNSI RCATGY 1 cut(s) 1339
BstPI GGTNACC 1 cut(s) 1260
BstSCI CCNGG 4 cut(s) 847, 970, 1155, 1914
BstSFI CTRYAG 1 cut(s) 1321
BstSLI GKGCMC 3 cut(s) 1054, 1257, 2118
BstV1I GCAGC 5 cut(s) 433, 802, 942, 1534, 1685
BstV2I GAAGAC 2 cut(s) 1800, 2382
BstX2I RGATCY 2 cut(s) 119, 686
BstYI RGATCY 2 cut(s) 119, 686
Bsu15I ATCGAT 1 cut(s) 1400
BsuRI GGCC 5 cut(s) 627, 652, 1305, 1592, 1914
BsuTUI ATCGAT 1 cut(s) 1400
BtgI CCRYGG 2 cut(s) 264, 659
BtsCI GGATG 5 cut(s) 279, 810, 814, 2149, 2284
BtsI GCAGTG 1 cut(s) 1902
BtsIMutI CAGTG 4 cut(s) 1558, 1902, 1954, 2369
BveI ACCTGC 2 cut(s) 417, 1273
Cac8I GCNNGC 5 cut(s) 184, 444, 513, 968, 1388
CaiI CAGNNNCTG 1 cut(s) 2303
CfoI GCGC 2 cut(s) 451, 517
Cfr10I RCCGGY 4 cut(s) 292, 425, 1018, 1257
Cfr13I GGNCC 5 cut(s) 375, 403, 626, 845, 1303
ClaI ATCGAT 1 cut(s) 1400
CseI GACGC 3 cut(s) 176, 653, 1102
Csp6I GTAC 6 cut(s) 249, 945, 951, 1173, 1642, 1822
CspAI ACCGGT 1 cut(s) 1257
CspCI CAANNNNNGTGG 2 cut(s) 1189, 1224
CviQI GTAC 6 cut(s) 249, 945, 951, 1173, 1642, 1822
DdeI CTNAG 5 cut(s) 312, 416, 507, 1342, 2304
DpnI GATC 7 cut(s) 121, 657, 688, 986, 1399, 1662, 1679
DpnII GATC 7 cut(s) 119, 655, 686, 984, 1397, 1660, 1677
DraI TTTAAA 1 cut(s) 1606
DraIII CACNNNGTG 1 cut(s) 372
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
Eam1104I CTCTTC 5 cut(s) 612, 1605, 1874, 1926, 2364
EarI CTCTTC 5 cut(s) 612, 1605, 1874, 1926, 2364
Eco130I CCWWGG 3 cut(s) 659, 1233, 1431
Eco147I AGGCCT 1 cut(s) 1914
Eco47I GGWCC 3 cut(s) 375, 403, 845
Eco47III AGCGCT 1 cut(s) 516
Eco57I CTGAAG 2 cut(s) 1604, 2345
Eco88I CYCGRG 1 cut(s) 629
Eco91I GGTNACC 1 cut(s) 1260
EcoNI CCTNNNNNAGG 1 cut(s) 945
EcoO109I RGGNCCY 2 cut(s) 845, 1303
EcoO65I GGTNACC 1 cut(s) 1260
EcoRII CCWGG 3 cut(s) 847, 1155, 1914
EcoT14I CCWWGG 3 cut(s) 659, 1233, 1431
ErhI CCWWGG 3 cut(s) 659, 1233, 1431
FauNDI CATATG 3 cut(s) 612, 1375, 1758
FbaI TGATCA 2 cut(s) 984, 1660
Fnu4HI GCNGC 8 cut(s) 447, 457, 460, 791, 931, 1548, 1674, 1970
FokI GGATG 5 cut(s) 286, 817, 821, 2156, 2291
Fsp4HI GCNGC 8 cut(s) 447, 457, 460, 791, 931, 1548, 1674, 1970
FspBI CTAG 5 cut(s) 522, 942, 1517, 1811, 1898
FspI TGCGCA 1 cut(s) 450
GlaI GCGC 2 cut(s) 450, 516
GluI GCNGC 8 cut(s) 447, 457, 460, 791, 931, 1548, 1674, 1970
GsuI CTGGAG 1 cut(s) 870
HaeII RGCGCY 1 cut(s) 518
HaeIII GGCC 5 cut(s) 627, 652, 1305, 1592, 1914
HgaI GACGC 3 cut(s) 176, 653, 1102
HhaI GCGC 2 cut(s) 451, 517
Hin1I GRCGYC 2 cut(s) 664, 1113
Hin6I GCGC 2 cut(s) 449, 515
HinP1I GCGC 2 cut(s) 449, 515
HincII GTYRAC 2 cut(s) 298, 305
HindII GTYRAC 2 cut(s) 298, 305
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 6 cut(s) 708, 1179, 1508, 1557, 1988, 2401
HphI GGTGA 8 cut(s) 212, 253, 310, 547, 1254, 1721, 2057, 2327
Hpy188III TCNNGA 6 cut(s) 164, 284, 546, 802, 2105, 2429
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 4 cut(s) 1550, 1557, 1582, 2216
HpyCH4IV ACGT 2 cut(s) 1084, 2208
HpyF10VI GCNNNNNNNGC 5 cut(s) 322, 448, 930, 1119, 2122
HpyF3I CTNAG 5 cut(s) 312, 416, 507, 1342, 2304
HpySE526I ACGT 2 cut(s) 1084, 2208
Hsp92I GRCGYC 2 cut(s) 664, 1113
HspAI GCGC 2 cut(s) 449, 515
Kpn2I TCCGGA 2 cut(s) 163, 2104
Ksp22I TGATCA 2 cut(s) 984, 1660
Kzo9I GATC 7 cut(s) 119, 655, 686, 984, 1397, 1660, 1677
LguI GCTCTTC 1 cut(s) 2364
LmnI GCTCC 6 cut(s) 235, 914, 1279, 1828, 2107, 2282
Lsp1109I GCAGC 5 cut(s) 433, 802, 942, 1534, 1685
LweI GCATC 6 cut(s) 752, 1084, 1433, 1464, 2166, 2406
MaeI CTAG 5 cut(s) 522, 942, 1517, 1811, 1898
MaeII ACGT 2 cut(s) 1084, 2208
MaeIII GTNAC 7 cut(s) 284, 830, 865, 1260, 1477, 2093, 2153
MalI GATC 7 cut(s) 121, 657, 688, 986, 1399, 1662, 1679
MboI GATC 7 cut(s) 119, 655, 686, 984, 1397, 1660, 1677
MflI RGATCY 2 cut(s) 119, 686
MhlI GDGCHC 4 cut(s) 1054, 1257, 1343, 2118
MlyI GAGTC 2 cut(s) 1982, 2410
MmeI TCCRAC 3 cut(s) 375, 405, 2016
MroI TCCGGA 2 cut(s) 163, 2104
MseI TTAA 4 cut(s) 105, 1605, 2036, 2349
MslI CAYNNNNRTG 3 cut(s) 266, 330, 1959
MspA1I CMGCKG 1 cut(s) 182
MspR9I CCNGG 4 cut(s) 849, 972, 1157, 1916
MvaI CCWGG 3 cut(s) 849, 1157, 1916
MwoI GCNNNNNNNGC 5 cut(s) 322, 448, 930, 1119, 2122
NciI CCSGG 1 cut(s) 972
NcoI CCATGG 1 cut(s) 659
NdeI CATATG 3 cut(s) 612, 1375, 1758
NdeII GATC 7 cut(s) 119, 655, 686, 984, 1397, 1660, 1677
NlaIV GGNNCC 6 cut(s) 121, 237, 628, 1256, 2086, 2103
NmuCI GTSAC 3 cut(s) 284, 1260, 1477
NsbI TGCGCA 1 cut(s) 450
NspI RCATGY 1 cut(s) 1339
OliI CACNNNNGTG 2 cut(s) 266, 330
PaqCI CACCTGC 2 cut(s) 417, 1273
PceI AGGCCT 1 cut(s) 1914
PciI ACATGT 1 cut(s) 1335
PciSI GCTCTTC 1 cut(s) 2364
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PfeI GAWTC 4 cut(s) 708, 1179, 1508, 1557
PfoI TCCNGGA 1 cut(s) 847
PinAI ACCGGT 1 cut(s) 1257
PkrI GCNGC 8 cut(s) 448, 458, 461, 792, 932, 1549, 1675, 1971
PleI GAGTC 2 cut(s) 1982, 2409
PpsI GAGTC 2 cut(s) 1982, 2409
Ppu21I YACGTR 1 cut(s) 1085
PpuMI RGGWCCY 1 cut(s) 845
PscI ACATGT 1 cut(s) 1335
Psp1406I AACGTT 1 cut(s) 2208
Psp5II RGGWCCY 1 cut(s) 845
Psp6I CCWGG 3 cut(s) 847, 1155, 1914
PspEI GGTNACC 1 cut(s) 1260
PspGI CCWGG 3 cut(s) 847, 1155, 1914
PspN4I GGNNCC 6 cut(s) 121, 237, 628, 1256, 2086, 2103
PspPI GGNCC 5 cut(s) 375, 403, 626, 845, 1303
PspPPI RGGWCCY 1 cut(s) 845
PsrI GAACNNNNNNTAC 2 cut(s) 1506, 1538
PstNI CAGNNNCTG 1 cut(s) 2303
PsuI RGATCY 2 cut(s) 119, 686
RsaI GTAC 6 cut(s) 250, 946, 952, 1174, 1643, 1823
RsaNI GTAC 6 cut(s) 249, 945, 951, 1173, 1642, 1822
RseI CAYNNNNRTG 3 cut(s) 266, 330, 1959
SapI GCTCTTC 1 cut(s) 2364
SaqAI TTAA 4 cut(s) 105, 1605, 2036, 2349
SatI GCNGC 8 cut(s) 447, 457, 460, 791, 931, 1548, 1674, 1970
Sau3AI GATC 7 cut(s) 119, 655, 686, 984, 1397, 1660, 1677
Sau96I GGNCC 5 cut(s) 375, 403, 626, 845, 1303
ScaI AGTACT 1 cut(s) 250
SchI GAGTC 2 cut(s) 1982, 2410
ScrFI CCNGG 4 cut(s) 849, 972, 1157, 1916
SduI GDGCHC 4 cut(s) 1054, 1257, 1343, 2118
SfaNI GCATC 6 cut(s) 752, 1084, 1433, 1464, 2166, 2406
SfcI CTRYAG 1 cut(s) 1321
SinI GGWCC 3 cut(s) 375, 403, 845
SmiMI CAYNNNNRTG 3 cut(s) 266, 330, 1959
SmlI CTYRAG 1 cut(s) 1530
SmoI CTYRAG 1 cut(s) 1530
SseBI AGGCCT 1 cut(s) 1914
SsiI CCGC 6 cut(s) 180, 456, 459, 912, 1491, 1970
SspI AATATT 1 cut(s) 798
SspMI CTAG 5 cut(s) 522, 942, 1517, 1811, 1898
StuI AGGCCT 1 cut(s) 1914
StyD4I CCNGG 4 cut(s) 847, 970, 1155, 1914
StyI CCWWGG 3 cut(s) 659, 1233, 1431
TaiI ACGT 2 cut(s) 1087, 2211
TaqI TCGA 8 cut(s) 357, 591, 638, 706, 771, 838, 1400, 2404
TaqII GACCGA 2 cut(s) 375, 391
TatI WGTACW 5 cut(s) 248, 944, 1172, 1641, 1821
TauI GCSGC 3 cut(s) 459, 462, 1972
TfiI GAWTC 4 cut(s) 708, 1179, 1508, 1557
Tru1I TTAA 4 cut(s) 105, 1605, 2036, 2349
Tru9I TTAA 4 cut(s) 105, 1605, 2036, 2349
TscAI CASTG 4 cut(s) 1558, 1909, 1961, 2369
TseFI GTSAC 3 cut(s) 284, 1260, 1477
TseI GCWGC 5 cut(s) 446, 790, 930, 1547, 1673
Tsp45I GTSAC 3 cut(s) 284, 1260, 1477
TspDTI ATGAA 8 cut(s) 599, 890, 993, 1392, 1775, 1902, 1944, 2087
TspGWI ACGGA 2 cut(s) 506, 1023
TspRI CASTG 4 cut(s) 1558, 1909, 1961, 2369
VneI GTGCAC 2 cut(s) 1050, 2114
VpaK11BI GGWCC 3 cut(s) 375, 403, 845
XagI CCTNNNNNAGG 1 cut(s) 945
XapI RAATTY 8 cut(s) 733, 784, 897, 1190, 1202, 1418, 1849, 2340
XceI RCATGY 1 cut(s) 1339
XcmI CCANNNNNNNNNTGG 2 cut(s) 1190, 2095
XspI CTAG 5 cut(s) 522, 942, 1517, 1811, 1898
ZrmI AGTACT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.