Rorug06G0477800

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009328
Physical Location & Seq
Reverse (-)
59945834 .. 59947795
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0477800.1

Sequence Viewer

Length: 930 bp
ATGGCTTCTTCTTCTTCGACCAAGAAAGCCGCGGCGGATCGGATAAAAGGTCCGTGGAGCCCAGAGGAAGACGAAGCGTTACAGAGGCTGGTCCAGAGCTACGGCCCGAGGAACTGGTCTCTGATCAGCAAATCGATCCCCGGTCGATCCGGCAAGTCGTGCCGGCTACGGTGGTGCAACCAGCTCTCGCCGGAGGTGGAGCACCGGCCGTTTACTCCTGAAGAGGACGACACCATAATTCGAGCCCATGCCCGGTTCGGAAACAAGTGGGCCACCATCTCTCGCTTGCTCAATGGCCGCACCGATAACGCCATTAAGAACCACTGGAACTCCACGCTCAAGCGCAAATTCTCGTCCTTCTCCGACGACATGTCGCCCGAGCAGCCTCTCAAAAGATCGGCCAGCGTCGGAGCGGGTACAACAGTGTCGGGTCTCTACATGAACCCGGGTAGCCCATCCGGATCCGATTTGAGCGATTCTAGTTTACCCGGCGTGATTCATCCCTCGCAAGCCTTCCGGTCGGTGGAGCCGGTAGCTTTGGCGACGGATCCGCCGACATCGCTCAGCCTGTCGCTTCCCGGATCCAATGAAGGGCCGAATCATCAGAGGTCAGCATCCGGATCCGGATCCAGTTGGGTTGTGAGCCCACAGCCCAAGGCCCAGATAGTTCAACTTCAACCTCAAGTAATTCAAGCTCAACCTGAAGAAGCTGGAGGTGGAGCGGCGGCGCGTCAGGTCAACCAAAACAGTGAGGTGGGGATTGATAAGCAGTTCTTCAGCGCAGAGTTCTTGGATGTGATGCAAGAGATGATCAGAAAGGAAGTGAGAAACTATATGTCTGGGGTTGATCACAATGGGATGTTCATGCACACTGAAGCTGCCATTAGAAACGCAGTTGTAAAGCGGATTGGGATTAGCAAAGTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

309

Amino Acids

33.62

Weight (kDa)

9.07

Isoelectric Point (pI)

59.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 16 - 62 1e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 19 - 76 1.5e-17 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 69 - 112 3.9e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 122 6.9e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 413, 722
AccII CGCG 2 cut(s) 32, 730
AccIII TCCGGA 3 cut(s) 458, 617, 623
AciI CCGC 9 cut(s) 30, 32, 35, 298, 413, 551, 722, 725, 904
AcoI YGGCCR 3 cut(s) 206, 295, 399
AcsI RAATTY 1 cut(s) 347
AcuI CTGAAG 4 cut(s) 240, 723, 760, 894
AfaI GTAC 1 cut(s) 418
AfiI CCNNNNNNNGG 3 cut(s) 252, 523, 591
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 3 cut(s) 671, 677, 692
AhdI GACNNNNNGTC 1 cut(s) 370
AluBI AGCT 6 cut(s) 99, 184, 536, 695, 710, 878
AluI AGCT 6 cut(s) 99, 184, 536, 695, 710, 878
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 123, 437
AlwNI CAGNNNCTG 1 cut(s) 88
Ama87I CYCGRG 3 cut(s) 106, 377, 445
Aor13HI TCCGGA 3 cut(s) 458, 617, 623
AoxI GGCC 7 cut(s) 103, 206, 270, 295, 399, 593, 657
ApeKI GCWGC 2 cut(s) 382, 878
ApoI RAATTY 1 cut(s) 347
AspLEI GCGC 3 cut(s) 345, 730, 782
AspS9I GGNCC 6 cut(s) 50, 91, 104, 270, 593, 658
AsuC2I CCSGG 6 cut(s) 141, 253, 446, 447, 489, 579
AvaI CYCGRG 3 cut(s) 106, 377, 445
AvaII GGWCC 2 cut(s) 50, 91
BaeI ACNNNNGTAYC 2 cut(s) 408, 441
BamHI GGATCC 5 cut(s) 461, 547, 581, 620, 626
BanII GRGCYC 3 cut(s) 62, 247, 647
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 394, 865
BccI CCATC 2 cut(s) 284, 463
BceAI ACGGC 2 cut(s) 118, 193
BclI TGATCA 3 cut(s) 123, 810, 847
BcnI CCSGG 6 cut(s) 141, 253, 446, 447, 489, 579
BcoDI GTCTC 2 cut(s) 123, 437
BfaI CTAG 1 cut(s) 480
BisI GCNGC 7 cut(s) 30, 33, 298, 383, 723, 726, 879
BlpI GCTNAGC 1 cut(s) 563
BlsI GCNGC 7 cut(s) 31, 34, 299, 384, 724, 727, 880
Bme1390I CCNGG 6 cut(s) 141, 253, 446, 447, 489, 579
Bme18I GGWCC 2 cut(s) 50, 91
BmeRI GACNNNNNGTC 1 cut(s) 370
BmeT110I CYCGRG 3 cut(s) 106, 377, 445
BmgT120I GGNCC 6 cut(s) 50, 91, 104, 270, 593, 658
BmiI GGNNCC 7 cut(s) 59, 463, 528, 549, 583, 622, 628
BmrFI CCNGG 6 cut(s) 141, 253, 446, 447, 489, 579
BmsI GCATC 2 cut(s) 623, 789
BpiI GAAGAC 1 cut(s) 75
BpmI CTGGAG 1 cut(s) 732
Bpu1102I GCTNAGC 1 cut(s) 563
BpuEI CTTGAG 2 cut(s) 323, 666
BpuMI CCSGG 6 cut(s) 141, 253, 446, 447, 489, 579
Bsa29I ATCGAT 1 cut(s) 134
BsaBI GATNNNNATC 1 cut(s) 625
BsaI GGTCTC 2 cut(s) 123, 437
BsaJI CCNNGG 6 cut(s) 30, 53, 107, 139, 445, 654
BsaWI WCCGGW 4 cut(s) 458, 516, 617, 623
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 3 cut(s) 252, 523, 591
Bse118I RCCGGY 3 cut(s) 162, 204, 529
Bse1I ACTGG 3 cut(s) 119, 329, 630
Bse8I GATNNNNATC 1 cut(s) 625
BseAI TCCGGA 3 cut(s) 458, 617, 623
BseCI ATCGAT 1 cut(s) 134
BseDI CCNNGG 6 cut(s) 30, 53, 107, 139, 445, 654
BseGI GGATG 5 cut(s) 455, 499, 614, 799, 864
BseJI GATNNNNATC 1 cut(s) 625
BseLI CCNNNNNNNGG 3 cut(s) 252, 523, 591
BseMII CTCAG 1 cut(s) 577
BseNI ACTGG 3 cut(s) 119, 329, 630
BseX3I CGGCCG 1 cut(s) 206
BseXI GCAGC 2 cut(s) 394, 865
Bsh1236I CGCG 2 cut(s) 32, 730
Bsh1285I CGRYCG 3 cut(s) 145, 209, 521
BshFI GGCC 7 cut(s) 105, 208, 272, 297, 401, 595, 659
BshVI ATCGAT 1 cut(s) 134
BsiEI CGRYCG 3 cut(s) 145, 209, 521
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 3 cut(s) 106, 377, 445
BslI CCNNNNNNNGG 3 cut(s) 252, 523, 591
BsmAI GTCTC 2 cut(s) 123, 437
BsnI GGCC 7 cut(s) 105, 208, 272, 297, 401, 595, 659
Bso31I GGTCTC 2 cut(s) 123, 437
BsoBI CYCGRG 3 cut(s) 106, 377, 445
Bsp1286I GDGCHC 4 cut(s) 62, 204, 247, 647
Bsp13I TCCGGA 3 cut(s) 458, 617, 623
Bsp1720I GCTNAGC 1 cut(s) 563
BspACI CCGC 9 cut(s) 30, 32, 35, 298, 413, 551, 722, 725, 904
BspANI GGCC 7 cut(s) 105, 208, 272, 297, 401, 595, 659
BspCNI CTCAG 1 cut(s) 576
BspDI ATCGAT 1 cut(s) 134
BspEI TCCGGA 3 cut(s) 458, 617, 623
BspFNI CGCG 2 cut(s) 32, 730
BspLI GGNNCC 7 cut(s) 59, 463, 528, 549, 583, 622, 628
BspTNI GGTCTC 2 cut(s) 123, 437
BsrBI CCGCTC 2 cut(s) 413, 722
BsrFI RCCGGY 3 cut(s) 162, 204, 529
BsrI ACTGG 3 cut(s) 119, 329, 630
BssAI RCCGGY 3 cut(s) 162, 204, 529
BssECI CCNNGG 6 cut(s) 30, 53, 107, 139, 445, 654
BssT1I CCWWGG 1 cut(s) 654
Bst4CI ACNGT 3 cut(s) 171, 424, 749
Bst6I CTCTTC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 159
BstC8I GCNNGC 4 cut(s) 164, 287, 403, 510
BstDEI CTNAG 1 cut(s) 563
BstDSI CCRYGG 2 cut(s) 30, 53
BstF5I GGATG 5 cut(s) 455, 499, 614, 799, 864
BstFNI CGCG 2 cut(s) 32, 730
BstHHI GCGC 3 cut(s) 345, 730, 782
BstMAI GTCTC 2 cut(s) 123, 437
BstMCI CGRYCG 3 cut(s) 145, 209, 521
BstMWI GCNNNNNNNGC 3 cut(s) 159, 382, 559
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 6 cut(s) 139, 251, 444, 445, 487, 577
BstUI CGCG 2 cut(s) 32, 730
BstV1I GCAGC 2 cut(s) 394, 865
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 5 cut(s) 461, 547, 581, 620, 626
BstYI RGATCY 5 cut(s) 461, 547, 581, 620, 626
BstZI CGGCCG 1 cut(s) 206
Bsu15I ATCGAT 1 cut(s) 134
BsuRI GGCC 7 cut(s) 105, 208, 272, 297, 401, 595, 659
BsuTUI ATCGAT 1 cut(s) 134
BtgI CCRYGG 2 cut(s) 30, 53
BtgZI GCGATG 1 cut(s) 543
BtsCI GGATG 5 cut(s) 455, 499, 614, 799, 864
BtsIMutI CAGTG 4 cut(s) 322, 429, 754, 870
Cac8I GCNNGC 4 cut(s) 164, 287, 403, 510
CaiI CAGNNNCTG 1 cut(s) 88
CfoI GCGC 3 cut(s) 345, 730, 782
Cfr10I RCCGGY 3 cut(s) 162, 204, 529
Cfr13I GGNCC 6 cut(s) 50, 91, 104, 270, 593, 658
Cfr42I CCGCGG 1 cut(s) 33
Cfr9I CCCGGG 1 cut(s) 445
ClaI ATCGAT 1 cut(s) 134
CseI GACGC 2 cut(s) 394, 719
Csp6I GTAC 1 cut(s) 417
CviAII CATG 4 cut(s) 248, 370, 439, 865
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 1 cut(s) 563
DriI GACNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 3 cut(s) 206, 295, 399
EagI CGGCCG 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 216
Eam1105I GACNNNNNGTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 216
EciI GGCGGA 2 cut(s) 50, 540
EclXI CGGCCG 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 654
Eco24I GRGCYC 3 cut(s) 62, 247, 647
Eco31I GGTCTC 2 cut(s) 123, 437
Eco47I GGWCC 2 cut(s) 50, 91
Eco52I CGGCCG 1 cut(s) 206
Eco57I CTGAAG 4 cut(s) 240, 723, 760, 894
Eco88I CYCGRG 3 cut(s) 106, 377, 445
EcoT14I CCWWGG 1 cut(s) 654
EcoT38I GRGCYC 3 cut(s) 62, 247, 647
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 4 cut(s) 251, 373, 442, 868
FaiI YATR 7 cut(s) 236, 249, 371, 440, 834, 836, 866
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FatI CATG 4 cut(s) 247, 369, 438, 864
FauI CCCGC 1 cut(s) 406
FbaI TGATCA 3 cut(s) 123, 810, 847
Fnu4HI GCNGC 7 cut(s) 30, 33, 298, 383, 723, 726, 879
FokI GGATG 5 cut(s) 442, 486, 601, 806, 871
FriOI GRGCYC 3 cut(s) 62, 247, 647
Fsp4HI GCNGC 7 cut(s) 30, 33, 298, 383, 723, 726, 879
FspBI CTAG 1 cut(s) 480
GlaI GCGC 3 cut(s) 344, 729, 781
GluI GCNGC 7 cut(s) 30, 33, 298, 383, 723, 726, 879
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 7 cut(s) 105, 208, 272, 297, 401, 595, 659
HgaI GACGC 2 cut(s) 394, 719
HhaI GCGC 3 cut(s) 345, 730, 782
Hin1II CATG 4 cut(s) 251, 373, 442, 868
Hin6I GCGC 3 cut(s) 343, 728, 780
HinP1I GCGC 3 cut(s) 343, 728, 780
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 3 cut(s) 476, 496, 598
Hpy166II GTNNAC 3 cut(s) 213, 485, 739
Hpy188I TCNGA 8 cut(s) 42, 123, 260, 364, 410, 466, 606, 815
Hpy188III TCNNGA 5 cut(s) 94, 218, 459, 618, 624
Hpy8I GTNNAC 3 cut(s) 213, 485, 739
Hpy99I CGWCG 3 cut(s) 368, 410, 547
HpyAV CCTTC 3 cut(s) 367, 523, 584
HpyCH4III ACNGT 3 cut(s) 171, 424, 749
HpyCH4V TGCA 3 cut(s) 177, 802, 868
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 382, 559
HpyF3I CTNAG 1 cut(s) 563
Hsp92II CATG 4 cut(s) 251, 373, 442, 868
HspAI GCGC 3 cut(s) 343, 728, 780
Kpn2I TCCGGA 3 cut(s) 458, 617, 623
KroI GCCGGC 1 cut(s) 162
KroNI GCCGGC 1 cut(s) 164
Ksp22I TGATCA 3 cut(s) 123, 810, 847
KspI CCGCGG 1 cut(s) 33
LmnI GCTCC 5 cut(s) 57, 199, 410, 526, 719
Lsp1109I GCAGC 2 cut(s) 394, 865
LweI GCATC 2 cut(s) 623, 789
MaeI CTAG 1 cut(s) 480
MaeIII GTNAC 1 cut(s) 78
MbiI CCGCTC 2 cut(s) 413, 722
MboII GAAGA 6 cut(s) 3, 6, 80, 233, 716, 766
MflI RGATCY 5 cut(s) 461, 547, 581, 620, 626
MhlI GDGCHC 4 cut(s) 62, 204, 247, 647
MluCI AATT 3 cut(s) 237, 347, 687
MmeI TCCRAC 2 cut(s) 387, 388
MroI TCCGGA 3 cut(s) 458, 617, 623
MroNI GCCGGC 1 cut(s) 162
MseI TTAA 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 6 cut(s) 141, 253, 446, 447, 489, 579
MvnI CGCG 2 cut(s) 32, 730
MwoI GCNNNNNNNGC 3 cut(s) 159, 382, 559
NaeI GCCGGC 1 cut(s) 164
NciI CCSGG 6 cut(s) 141, 253, 446, 447, 489, 579
NgoMIV GCCGGC 1 cut(s) 162
NlaIII CATG 4 cut(s) 251, 373, 442, 868
NlaIV GGNNCC 7 cut(s) 59, 463, 528, 549, 583, 622, 628
NspI RCATGY 1 cut(s) 373
PciI ACATGT 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 551
PdiI GCCGGC 1 cut(s) 164
PfeI GAWTC 3 cut(s) 476, 496, 598
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 7 cut(s) 31, 34, 299, 384, 724, 727, 880
PscI ACATGT 1 cut(s) 369
PspN4I GGNNCC 7 cut(s) 59, 463, 528, 549, 583, 622, 628
PspPI GGNCC 6 cut(s) 50, 91, 104, 270, 593, 658
PstNI CAGNNNCTG 1 cut(s) 88
PsuI RGATCY 5 cut(s) 461, 547, 581, 620, 626
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
SacII CCGCGG 1 cut(s) 33
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 7 cut(s) 30, 33, 298, 383, 723, 726, 879
Sau96I GGNCC 6 cut(s) 50, 91, 104, 270, 593, 658
ScrFI CCNGG 6 cut(s) 141, 253, 446, 447, 489, 579
SduI GDGCHC 4 cut(s) 62, 204, 247, 647
SfaNI GCATC 2 cut(s) 623, 789
Sfr303I CCGCGG 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 33
SinI GGWCC 2 cut(s) 50, 91
SmaI CCCGGG 1 cut(s) 447
SmlI CTYRAG 2 cut(s) 338, 681
SmoI CTYRAG 2 cut(s) 338, 681
Sse9I AATT 3 cut(s) 237, 347, 687
SsiI CCGC 9 cut(s) 30, 32, 35, 298, 413, 551, 722, 725, 904
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 6 cut(s) 139, 251, 444, 445, 487, 577
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 3 cut(s) 171, 424, 749
TaqI TCGA 4 cut(s) 17, 134, 145, 241
TasI AATT 3 cut(s) 237, 347, 687
TauI GCSGC 5 cut(s) 32, 35, 300, 725, 728
TfiI GAWTC 3 cut(s) 476, 496, 598
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 4 cut(s) 329, 429, 754, 877
TseI GCWGC 2 cut(s) 382, 878
TspDTI ATGAA 4 cut(s) 455, 488, 603, 853
TspGWI ACGGA 2 cut(s) 42, 560
TspMI CCCGGG 1 cut(s) 445
TspRI CASTG 4 cut(s) 329, 429, 754, 877
VpaK11BI GGWCC 2 cut(s) 50, 91
XapI RAATTY 1 cut(s) 347
XceI RCATGY 1 cut(s) 373
XmaI CCCGGG 1 cut(s) 445
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.