Rorug07G0015100

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0007008
Physical Location & Seq
Forward (+)
1191912 .. 1192743
832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0015100.1

Sequence Viewer

Length: 495 bp
ATGGCTTCCAACGGAACCCCTACCACCGCCGACGCCAAACCCATCGTGACCGACGAGGTCCGCCGCGTCTTCAAGAGCTTCGACGCCAACGGCGACGGCAAGATCTCAACCTCCGAGCTCGGCAACGTCCTCGCGGCCCTCGGCTCCAACGTCCCCGAGGACGAGCTCCAGCGCGTGATGGTCGACCTCGACACCGACCGCGACGGCTTCATCTGCATCGACGAGTTCAACGCCTTCTGGGTGGCCGGCGACGGCGGCTCCTCCGAGCTCAAGGAGGCCTTCGATCTGTACGACCAGGACCGCAACGGCCTCATCTCCGTCGATGAGCTCCACCTCGTCCTCAACCGGCTTCAGATGAAGTGCACCGTTGAGGATTGCAACCGCATGATCCAGTCCGTTGACGCCGACGGCGACGGGAATGTGAATTTCGAGGAGTTCAAGAAGATGATGAGCCACAACTCCAGCAACGGCGGCGCCGTCTCTAACGGCGCCTGA

Protein Analysis

164

Amino Acids

17.77

Weight (kDa)

4.26

Isoelectric Point (pI)

27.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 19 - 46 6.5e-07 EF hand domain
EF-hand_7 PF13499 19 - 79 1e-13 EF-hand domain pair
EF-hand_6 PF13405 19 - 48 1.5e-07 EF-hand domain
EF-hand_5 PF13202 22 - 43 9e-07 EF hand
EF-hand_7 PF13499 88 - 151 4.3e-15 EF-hand domain pair
EF-hand_1 PF00036 89 - 116 1e-06 EF hand domain
EF-hand_6 PF13405 89 - 116 6.2e-07 EF-hand domain
EF-hand_1 PF00036 126 - 152 1.4e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 473, 488
AccI GTMKAC 1 cut(s) 183
AccII CGCG 4 cut(s) 66, 134, 174, 201
AciI CCGC 9 cut(s) 27, 61, 64, 134, 199, 255, 301, 382, 471
AclWI GGATC 1 cut(s) 382
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 424
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 5 cut(s) 33, 84, 402, 474, 489
AfaI GTAC 1 cut(s) 290
AgsI TTSAA 3 cut(s) 73, 229, 439
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 5 cut(s) 78, 118, 166, 268, 328
AluI AGCT 5 cut(s) 78, 118, 166, 268, 328
Alw21I GWGCWC 5 cut(s) 120, 168, 270, 330, 365
Alw26I GTCTC 1 cut(s) 484
Alw44I GTGCAC 1 cut(s) 361
AlwI GGATC 1 cut(s) 382
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 4 cut(s) 135, 243, 276, 307
ApaLI GTGCAC 1 cut(s) 361
ApoI RAATTY 1 cut(s) 424
AspLEI GCGC 3 cut(s) 174, 476, 491
AspS9I GGNCC 3 cut(s) 58, 136, 298
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 2 cut(s) 58, 298
BaeGI GKGCMC 1 cut(s) 365
BanI GGYRCC 2 cut(s) 473, 488
BanII GRGCYC 4 cut(s) 120, 168, 270, 330
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 5 cut(s) 120, 168, 270, 330, 365
BccI CCATC 2 cut(s) 50, 172
BceAI ACGGC 8 cut(s) 106, 112, 220, 268, 322, 424, 461, 484
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciT130I CCWGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 484
BfoI RGCGCY 2 cut(s) 477, 492
BglII AGATCT 1 cut(s) 102
BisI GCNGC 4 cut(s) 64, 135, 256, 472
BlsI GCNGC 4 cut(s) 65, 136, 257, 473
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 58, 298
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 3 cut(s) 58, 136, 298
BmiI GGNNCC 5 cut(s) 16, 145, 259, 475, 490
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 61
BpmI CTGGAG 2 cut(s) 152, 445
BpuEI CTTGAG 1 cut(s) 254
BsaHI GRCGYC 5 cut(s) 33, 84, 402, 474, 489
BsaJI CCNNGG 2 cut(s) 139, 156
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bse118I RCCGGY 2 cut(s) 245, 345
Bse1I ACTGG 1 cut(s) 391
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 139, 156
BseNI ACTGG 1 cut(s) 391
BseRI GAGGAG 2 cut(s) 250, 446
BseSI GKGCMC 1 cut(s) 365
Bsh1236I CGCG 4 cut(s) 66, 134, 174, 201
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 4 cut(s) 137, 245, 278, 309
BshNI GGYRCC 2 cut(s) 473, 488
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 5 cut(s) 120, 168, 270, 330, 365
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 2 cut(s) 246, 346
BslFI GGGAC 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 484
BsmBI CGTCTC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 4 cut(s) 137, 245, 278, 309
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 5 cut(s) 120, 168, 270, 330, 365
Bsp143I GATC 3 cut(s) 102, 283, 387
BspACI CCGC 9 cut(s) 27, 61, 64, 134, 199, 255, 301, 382, 471
BspANI GGCC 4 cut(s) 137, 245, 278, 309
BspFNI CGCG 4 cut(s) 66, 134, 174, 201
BspLI GGNNCC 5 cut(s) 16, 145, 259, 475, 490
BspPI GGATC 1 cut(s) 382
BspT107I GGYRCC 2 cut(s) 473, 488
BsrFI RCCGGY 2 cut(s) 245, 345
BsrI ACTGG 1 cut(s) 391
BssAI RCCGGY 2 cut(s) 245, 345
BssECI CCNNGG 2 cut(s) 139, 156
BssMI GATC 3 cut(s) 102, 283, 387
BssNI GRCGYC 5 cut(s) 33, 84, 402, 474, 489
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 367
BstACI GRCGYC 5 cut(s) 33, 84, 402, 474, 489
BstC8I GCNNGC 1 cut(s) 247
BstFNI CGCG 4 cut(s) 66, 134, 174, 201
BstH2I RGCGCY 2 cut(s) 477, 492
BstHHI GCGC 3 cut(s) 174, 476, 491
BstKTI GATC 3 cut(s) 105, 286, 390
BstMAI GTCTC 1 cut(s) 484
BstMBI GATC 3 cut(s) 102, 283, 387
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 3 cut(s) 213, 255, 471
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 365
BstUI CGCG 4 cut(s) 66, 134, 174, 201
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 4 cut(s) 137, 245, 278, 309
Cac8I GCNNGC 1 cut(s) 247
CfoI GCGC 3 cut(s) 174, 476, 491
Cfr10I RCCGGY 2 cut(s) 245, 345
Cfr13I GGNCC 3 cut(s) 58, 136, 298
CseI GACGC 4 cut(s) 41, 55, 92, 410
Csp6I GTAC 1 cut(s) 289
CviAII CATG 1 cut(s) 385
CviQI GTAC 1 cut(s) 289
DinI GGCGCC 2 cut(s) 475, 490
DpnI GATC 3 cut(s) 104, 285, 389
DpnII GATC 3 cut(s) 102, 283, 387
EaeI YGGCCR 1 cut(s) 243
EciI GGCGGA 1 cut(s) 50
Ecl136II GAGCTC 4 cut(s) 118, 166, 268, 328
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 4 cut(s) 120, 168, 270, 330
Eco47I GGWCC 2 cut(s) 58, 298
Eco53kI GAGCTC 4 cut(s) 118, 166, 268, 328
Eco57I CTGAAG 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 4 cut(s) 118, 166, 268, 328
EcoRII CCWGG 1 cut(s) 294
EcoT38I GRGCYC 4 cut(s) 120, 168, 270, 330
EgeI GGCGCC 2 cut(s) 475, 490
EheI GGCGCC 2 cut(s) 475, 490
Esp3I CGTCTC 1 cut(s) 484
FaeI CATG 1 cut(s) 388
FaiI YATR 1 cut(s) 386
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 1 cut(s) 137
FatI CATG 1 cut(s) 384
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 64, 135, 256, 472
FriOI GRGCYC 4 cut(s) 120, 168, 270, 330
Fsp4HI GCNGC 4 cut(s) 64, 135, 256, 472
GlaI GCGC 3 cut(s) 173, 475, 490
GluI GCNGC 4 cut(s) 64, 135, 256, 472
GsuI CTGGAG 2 cut(s) 152, 445
HaeII RGCGCY 2 cut(s) 477, 492
HaeIII GGCC 4 cut(s) 137, 245, 278, 309
HapII CCGG 2 cut(s) 246, 346
HgaI GACGC 4 cut(s) 41, 55, 92, 410
HhaI GCGC 3 cut(s) 174, 476, 491
Hin1I GRCGYC 5 cut(s) 33, 84, 402, 474, 489
Hin1II CATG 1 cut(s) 388
Hin6I GCGC 3 cut(s) 172, 474, 489
HinP1I GCGC 3 cut(s) 172, 474, 489
HincII GTYRAC 2 cut(s) 184, 400
HindII GTYRAC 2 cut(s) 184, 400
HpaII CCGG 2 cut(s) 246, 346
Hpy166II GTNNAC 3 cut(s) 184, 363, 400
Hpy188I TCNGA 3 cut(s) 115, 265, 354
Hpy188III TCNNGA 3 cut(s) 46, 73, 439
Hpy8I GTNNAC 3 cut(s) 184, 363, 400
HpyAV CCTTC 2 cut(s) 244, 289
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4IV ACGT 2 cut(s) 126, 150
HpyCH4V TGCA 3 cut(s) 216, 363, 378
HpyF10VI GCNNNNNNNGC 3 cut(s) 213, 255, 471
HpySE526I ACGT 2 cut(s) 126, 150
Hsp92I GRCGYC 5 cut(s) 33, 84, 402, 474, 489
Hsp92II CATG 1 cut(s) 388
HspAI GCGC 3 cut(s) 172, 474, 489
KasI GGCGCC 2 cut(s) 473, 488
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
Kzo9I GATC 3 cut(s) 102, 283, 387
LmnI GCTCC 4 cut(s) 149, 171, 263, 333
LpnPI CCDG 8 cut(s) 182, 223, 259, 281, 308, 359, 404, 475
LweI GCATC 1 cut(s) 225
MaeII ACGT 2 cut(s) 126, 150
MaeIII GTNAC 1 cut(s) 46
MalI GATC 3 cut(s) 104, 285, 389
MboI GATC 3 cut(s) 102, 283, 387
MboII GAAGA 2 cut(s) 61, 454
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 5 cut(s) 120, 168, 270, 330, 365
MluCI AATT 1 cut(s) 424
Mly113I GGCGCC 2 cut(s) 474, 489
MmeI TCCRAC 2 cut(s) 33, 171
MroNI GCCGGC 1 cut(s) 245
MspI CCGG 2 cut(s) 246, 346
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 4 cut(s) 66, 134, 174, 201
MwoI GCNNNNNNNGC 3 cut(s) 213, 255, 471
NaeI GCCGGC 1 cut(s) 247
NarI GGCGCC 2 cut(s) 474, 489
NdeII GATC 3 cut(s) 102, 283, 387
NgoMIV GCCGGC 1 cut(s) 245
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 5 cut(s) 16, 145, 259, 475, 490
NmeAIII GCCGAG 2 cut(s) 99, 120
NmuCI GTSAC 1 cut(s) 46
PceI AGGCCT 1 cut(s) 278
PcsI WCGNNNNNNNCGW 6 cut(s) 51, 90, 147, 288, 408, 474
PdiI GCCGGC 1 cut(s) 247
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 4 cut(s) 65, 136, 257, 473
PluTI GGCGCC 2 cut(s) 477, 492
Psp124BI GAGCTC 4 cut(s) 120, 168, 270, 330
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 5 cut(s) 16, 145, 259, 475, 490
PspPI GGNCC 3 cut(s) 58, 136, 298
PsuI RGATCY 1 cut(s) 102
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SacI GAGCTC 4 cut(s) 120, 168, 270, 330
SalI GTCGAC 1 cut(s) 182
SatI GCNGC 4 cut(s) 64, 135, 256, 472
Sau3AI GATC 3 cut(s) 102, 283, 387
Sau96I GGNCC 3 cut(s) 58, 136, 298
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 5 cut(s) 120, 168, 270, 330, 365
SfaNI GCATC 1 cut(s) 225
SfoI GGCGCC 2 cut(s) 475, 490
SinI GGWCC 2 cut(s) 58, 298
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 1 cut(s) 424
SseBI AGGCCT 1 cut(s) 278
SsiI CCGC 9 cut(s) 27, 61, 64, 134, 199, 255, 301, 382, 471
SspDI GGCGCC 2 cut(s) 473, 488
SstI GAGCTC 4 cut(s) 120, 168, 270, 330
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 367
TaiI ACGT 2 cut(s) 129, 153
TaqI TCGA 7 cut(s) 81, 183, 189, 219, 282, 321, 429
TaqII GACCGA 1 cut(s) 65
TasI AATT 1 cut(s) 424
TauI GCSGC 4 cut(s) 66, 137, 258, 474
TseFI GTSAC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 2 cut(s) 199, 371
TspGWI ACGGA 3 cut(s) 27, 307, 385
Tth111I GACNNNGTC 1 cut(s) 56
VneI GTGCAC 1 cut(s) 361
VpaK11BI GGWCC 2 cut(s) 58, 298
XapI RAATTY 1 cut(s) 424
XmiI GTMKAC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.