Rorug07G0273000

Protein FAM135B-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002474
Physical Location & Seq
Reverse (-)
25903917 .. 25905332
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0273000.1

Sequence Viewer

Length: 1368 bp
ATGAGAATTGAGGCAGCCCCTCAGGGCTCTCTTGTCATCCAGCTTCCCGGCTTCAATGGCACTTTTCCGTCCAGCCACTATTCCGGATATGTAAGCATAGGCGCAAAGAATCTTTTCTACTACTTCGTAGTATCAGAAGGGAGCCCCGCAGACGATCCTCTTGTCTTGTGGCTCAATGGTGGACCCGGCTGCTCTAGCTTTGATGGATTCGTCTACGAACATGGGCCTTTCAATTTTGAACAAAAATCAAAAGAAGCATTCCCCACTTTACAGCTTAATCCTTACAGCTGGTCCAAGGTTTCCAACATACTGTATTTGGATTCTCCTGCTGGTGTTGGGTTTTCCTACTCACAAGACCCAAGCCAATATGTAACCGGGGATCTTCAAACTGCTTCCGACACCCACGCCTTCCTCCTCAGTTGGTTTAAGCAGTACCCCGAGTTTCTTTCCAATCCTTTTTACATTTCCGGGGAGTCTTATGCTGGAGTCTACGTCCCTACTCTTGCCTTTCAAGTTGCCACAGGTATACAAAATGGAACAAAACCAGTTGTAAATCTCAAGGGTTACATGGTGGGGAATGGAATCACAAACACAAACTTTGATGACAATGCTCTTGTCCCTTTTGCTCATGGGATGGCCCTCATTTCAGATCAAATCTATGAGGGAGTTATAGAAGAATGTGGGATCAACTACTCCAACTCATCTAGTCCCAAGTGCGATGAGAAACTTCAACTGGTTCGCGAGGCTCTTGGTGGACTAAACATGTACGATGTACTTGAGCCTTGTTACCACGACCCAGCTGGAACTTATTCAGGAAATGCTACTTCTACAACAACCAAAAGCAGCAACTTACCCTTAAGCTTCCAGCAACTAGGCAAGTCGTCTGAGAAGCCTCTCGGAGTGAGGAAGAGGATGTCACTCAATGTTCCATGCTTTAATGATGAAGTCGCAACTAAATGGCTGAACGACCCAGCAGTCAGGGAAGCAATTCATGCCAAGCCTGCAAGTGTGTCTGGTCCCTGGGAATTATGCACAGATAGAATAAGTTATAATTTTGGTGCTGGAAGCATGATCCCATACCACCGAAACCTTACAACCAAAGGATATAAAGCAATCATTTTCAGTGGAGACCATGATATGTCTGTGCCGTATACTGGAAGCCAGGCATGGACTAGTTCACTTGGTTATAAGGTTATAGACCAATGGAGGTCTTGGCGATCCAATGACCAAGTTGCAGGTTACTTGCAAGCATATGAAAACGATCTCACCTTTCTCACTGTCAAGGGAGCTGGGCACACCGTTCCTGAATACAAGCCACGAGAGGCACTCGATTTCTACAGCCGCTGGTTGGCAGGAAAACCGATATGA

Protein Analysis

455

Amino Acids

50.1

Weight (kDa)

5.41

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 16 - 451 1.4e-135 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1050, 1188
AasI GACNNNNNNGTC 1 cut(s) 974
Acc36I ACCTGC 1 cut(s) 1226
AccI GTMKAC 4 cut(s) 213, 489, 526, 1151
AccII CGCG 1 cut(s) 741
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 2 cut(s) 147, 1342
AclWI GGATC 5 cut(s) 149, 387, 692, 1066, 1212
AfaI GTAC 3 cut(s) 434, 767, 774
AfiI CCNNNNNNNGG 2 cut(s) 1154, 1348
AflII CTTAAG 1 cut(s) 856
AflIII ACRYGT 1 cut(s) 762
AgsI TTSAA 6 cut(s) 55, 232, 239, 386, 512, 731
AhlI ACTAGT 1 cut(s) 1172
AjnI CCWGG 2 cut(s) 1019, 1161
AluBI AGCT 7 cut(s) 43, 198, 274, 288, 800, 861, 1289
AluI AGCT 7 cut(s) 43, 198, 274, 288, 800, 861, 1289
Alw26I GTCTC 1 cut(s) 1122
AlwI GGATC 5 cut(s) 149, 387, 692, 1066, 1212
AlwNI CAGNNNCTG 1 cut(s) 1344
Ama87I CYCGRG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 2 cut(s) 224, 636
ApeKI GCWGC 3 cut(s) 14, 189, 843
Asp700I GAANNNNTTC 3 cut(s) 113, 808, 987
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 5 cut(s) 182, 224, 291, 637, 1016
AsuC2I CCSGG 4 cut(s) 48, 186, 376, 469
AsuHPI GGTGA 1 cut(s) 1258
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 3 cut(s) 182, 291, 1016
AxyI CCTNAGG 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 1296
BanII GRGCYC 2 cut(s) 29, 146
BauI CACGAG 1 cut(s) 1317
BbvI GCAGC 3 cut(s) 26, 176, 855
BccI CCATC 2 cut(s) 197, 628
BceAI ACGGC 1 cut(s) 1132
BciT130I CCWGG 2 cut(s) 1021, 1163
BcnI CCSGG 4 cut(s) 48, 186, 376, 469
BcoDI GTCTC 1 cut(s) 1122
BcuI ACTAGT 1 cut(s) 1172
BfaI CTAG 4 cut(s) 195, 705, 872, 1173
BfmI CTRYAG 1 cut(s) 1336
BfrI CTTAAG 1 cut(s) 856
BfuAI ACCTGC 1 cut(s) 1226
BisI GCNGC 4 cut(s) 15, 190, 844, 1342
BlsI GCNGC 4 cut(s) 16, 191, 845, 1343
Bme1390I CCNGG 6 cut(s) 48, 186, 376, 469, 1021, 1163
Bme18I GGWCC 3 cut(s) 182, 291, 1016
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 5 cut(s) 182, 224, 291, 637, 1016
BmiI GGNNCC 3 cut(s) 143, 184, 1018
BmrFI CCNGG 6 cut(s) 48, 186, 376, 469, 1021, 1163
BplI GAGNNNNNCTC 2 cut(s) 1311, 1343
BpmI CTGGAG 1 cut(s) 504
BpuEI CTTGAG 2 cut(s) 542, 797
BpuMI CCSGG 4 cut(s) 48, 186, 376, 469
BsaI GGTCTC 1 cut(s) 1122
BsaJI CCNNGG 5 cut(s) 294, 375, 468, 1019, 1020
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 2 cut(s) 1154, 1348
Bse1I ACTGG 3 cut(s) 545, 738, 1159
Bse21I CCTNAGG 1 cut(s) 21
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 2 cut(s) 1021, 1163
BseDI CCNNGG 5 cut(s) 294, 375, 468, 1019, 1020
BseGI GGATG 3 cut(s) 36, 639, 918
BseLI CCNNNNNNNGG 2 cut(s) 1154, 1348
BseMII CTCAG 3 cut(s) 35, 430, 876
BseNI ACTGG 3 cut(s) 545, 738, 1159
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 1296
BseXI GCAGC 3 cut(s) 26, 176, 855
BseYI CCCAGC 3 cut(s) 796, 970, 1289
Bsh1236I CGCG 1 cut(s) 741
BshFI GGCC 2 cut(s) 226, 638
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 5 cut(s) 48, 84, 186, 375, 468
BslFI GGGAC 4 cut(s) 479, 602, 693, 1002
BslI CCNNNNNNNGG 2 cut(s) 1154, 1348
BsmAI GTCTC 1 cut(s) 1122
BsmFI GGGAC 4 cut(s) 479, 602, 693, 1002
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 2 cut(s) 226, 638
Bso31I GGTCTC 1 cut(s) 1122
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 3 cut(s) 29, 146, 1296
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 7 cut(s) 154, 379, 649, 684, 1071, 1217, 1261
Bsp68I TCGCGA 1 cut(s) 741
BspACI CCGC 2 cut(s) 147, 1342
BspANI GGCC 2 cut(s) 226, 638
BspCNI CTCAG 3 cut(s) 34, 429, 877
BspEI TCCGGA 1 cut(s) 83
BspFNI CGCG 1 cut(s) 741
BspLI GGNNCC 3 cut(s) 143, 184, 1018
BspMI ACCTGC 1 cut(s) 1226
BspPI GGATC 5 cut(s) 149, 387, 692, 1066, 1212
BspTI CTTAAG 1 cut(s) 856
BspTNI GGTCTC 1 cut(s) 1122
BsrI ACTGG 3 cut(s) 545, 738, 1159
BssECI CCNNGG 5 cut(s) 294, 375, 468, 1019, 1020
BssMI GATC 7 cut(s) 154, 379, 649, 684, 1071, 1217, 1261
BssNAI GTATAC 2 cut(s) 527, 1152
BssSI CACGAG 1 cut(s) 1317
BssT1I CCWWGG 1 cut(s) 294
Bst1107I GTATAC 2 cut(s) 527, 1152
Bst2BI CACGAG 1 cut(s) 1317
Bst2UI CCWGG 2 cut(s) 1021, 1163
Bst4CI ACNGT 3 cut(s) 312, 1279, 1300
Bst6I CTCTTC 1 cut(s) 902
BstAFI CTTAAG 1 cut(s) 856
BstAPI GCANNNNNTGC 1 cut(s) 992
BstC8I GCNNGC 2 cut(s) 1002, 1248
BstDEI CTNAG 3 cut(s) 21, 416, 885
BstF5I GGATG 3 cut(s) 36, 639, 918
BstFNI CGCG 1 cut(s) 741
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 7 cut(s) 157, 382, 652, 687, 1074, 1220, 1264
BstMAI GTCTC 1 cut(s) 1122
BstMBI GATC 7 cut(s) 154, 379, 649, 684, 1071, 1217, 1261
BstMWI GCNNNNNNNGC 4 cut(s) 57, 195, 992, 1001
BstNI CCWGG 2 cut(s) 1021, 1163
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 6 cut(s) 46, 184, 374, 467, 1019, 1161
BstSFI CTRYAG 1 cut(s) 1336
BstSLI GKGCMC 1 cut(s) 1296
BstUI CGCG 1 cut(s) 741
BstV1I GCAGC 3 cut(s) 26, 176, 855
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BstZ17I GTATAC 2 cut(s) 527, 1152
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 2 cut(s) 226, 638
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 3 cut(s) 36, 639, 918
BtsIMutI CAGTG 2 cut(s) 1129, 1275
BtuMI TCGCGA 1 cut(s) 741
BveI ACCTGC 1 cut(s) 1226
Cac8I GCNNGC 2 cut(s) 1002, 1248
CaiI CAGNNNCTG 1 cut(s) 1344
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 5 cut(s) 182, 224, 291, 637, 1016
Csp6I GTAC 3 cut(s) 433, 766, 773
CviAII CATG 9 cut(s) 221, 568, 629, 763, 930, 992, 1069, 1133, 1167
CviQI GTAC 3 cut(s) 433, 766, 773
DdeI CTNAG 3 cut(s) 21, 416, 885
DpnI GATC 7 cut(s) 156, 381, 651, 686, 1073, 1219, 1263
DpnII GATC 7 cut(s) 154, 379, 649, 684, 1071, 1217, 1261
DrdI GACNNNNNNGTC 1 cut(s) 974
DseDI GACNNNNNNGTC 1 cut(s) 974
Eam1104I CTCTTC 1 cut(s) 902
EarI CTCTTC 1 cut(s) 902
Eco130I CCWWGG 1 cut(s) 294
Eco24I GRGCYC 2 cut(s) 29, 146
Eco31I GGTCTC 1 cut(s) 1122
Eco47I GGWCC 3 cut(s) 182, 291, 1016
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 1019, 1161
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 29, 146
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 9 cut(s) 224, 571, 632, 766, 933, 995, 1072, 1136, 1170
FaqI GGGAC 4 cut(s) 479, 602, 693, 1002
FatI CATG 9 cut(s) 220, 567, 628, 762, 929, 991, 1068, 1132, 1166
FauI CCCGC 1 cut(s) 154
FauNDI CATATG 1 cut(s) 1252
FblI GTMKAC 4 cut(s) 213, 489, 526, 1151
Fnu4HI GCNGC 4 cut(s) 15, 190, 844, 1342
FokI GGATG 3 cut(s) 23, 646, 925
FriOI GRGCYC 2 cut(s) 29, 146
Fsp4HI GCNGC 4 cut(s) 15, 190, 844, 1342
FspBI CTAG 4 cut(s) 195, 705, 872, 1173
GlaI GCGC 1 cut(s) 103
GluI GCNGC 4 cut(s) 15, 190, 844, 1342
GsaI CCCAGC 3 cut(s) 800, 974, 1293
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 2 cut(s) 226, 638
HapII CCGG 5 cut(s) 48, 84, 186, 375, 468
HhaI GCGC 1 cut(s) 104
Hin1II CATG 9 cut(s) 224, 571, 632, 766, 933, 995, 1072, 1136, 1170
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HindIII AAGCTT 1 cut(s) 859
HinfI GANTC 6 cut(s) 109, 207, 320, 473, 486, 582
HpaII CCGG 5 cut(s) 48, 84, 186, 375, 468
HphI GGTGA 1 cut(s) 1258
Hpy166II GTNNAC 7 cut(s) 182, 214, 490, 527, 755, 1152, 1178
Hpy188I TCNGA 5 cut(s) 136, 397, 649, 886, 899
Hpy188III TCNNGA 4 cut(s) 84, 740, 813, 1304
Hpy8I GTNNAC 7 cut(s) 182, 214, 490, 527, 755, 1152, 1178
HpyAV CCTTC 2 cut(s) 131, 418
HpyCH4III ACNGT 3 cut(s) 312, 1279, 1300
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 4 cut(s) 1004, 1032, 1235, 1246
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 195, 992, 1001
HpyF3I CTNAG 3 cut(s) 21, 416, 885
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 9 cut(s) 224, 571, 632, 766, 933, 995, 1072, 1136, 1170
HspAI GCGC 1 cut(s) 102
Kpn2I TCCGGA 1 cut(s) 83
Kzo9I GATC 7 cut(s) 154, 379, 649, 684, 1071, 1217, 1261
LmnI GCTCC 2 cut(s) 141, 1286
Lsp1109I GCAGC 3 cut(s) 26, 176, 855
MaeI CTAG 4 cut(s) 195, 705, 872, 1173
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 5 cut(s) 370, 563, 785, 915, 1238
MalI GATC 7 cut(s) 156, 381, 651, 686, 1073, 1219, 1263
MboI GATC 7 cut(s) 154, 379, 649, 684, 1071, 1217, 1261
MboII GAAGA 3 cut(s) 374, 686, 919
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 3 cut(s) 29, 146, 1296
MluCI AATT 5 cut(s) 6, 232, 987, 1025, 1051
MlyI GAGTC 2 cut(s) 482, 495
MmeI TCCRAC 3 cut(s) 327, 420, 720
MroI TCCGGA 1 cut(s) 83
MroXI GAANNNNTTC 3 cut(s) 113, 808, 987
MseI TTAA 4 cut(s) 276, 426, 857, 936
MspA1I CMGCKG 3 cut(s) 288, 800, 1344
MspCI CTTAAG 1 cut(s) 856
MspI CCGG 5 cut(s) 48, 84, 186, 375, 468
MspR9I CCNGG 6 cut(s) 48, 186, 376, 469, 1021, 1163
Mva1269I GAATGC 1 cut(s) 257
MvaI CCWGG 2 cut(s) 1021, 1163
MvnI CGCG 1 cut(s) 741
MwoI GCNNNNNNNGC 4 cut(s) 57, 195, 992, 1001
NciI CCSGG 4 cut(s) 48, 186, 376, 469
NdeI CATATG 1 cut(s) 1252
NdeII GATC 7 cut(s) 154, 379, 649, 684, 1071, 1217, 1261
NlaIII CATG 9 cut(s) 224, 571, 632, 766, 933, 995, 1072, 1136, 1170
NlaIV GGNNCC 3 cut(s) 143, 184, 1018
NmuCI GTSAC 1 cut(s) 915
NruI TCGCGA 1 cut(s) 741
NspI RCATGY 1 cut(s) 766
PasI CCCWGGG 1 cut(s) 1020
PciI ACATGT 1 cut(s) 762
PctI GAATGC 1 cut(s) 257
PdmI GAANNNNTTC 3 cut(s) 113, 808, 987
PfeI GAWTC 4 cut(s) 109, 207, 320, 582
PkrI GCNGC 4 cut(s) 16, 191, 845, 1343
PleI GAGTC 2 cut(s) 481, 494
PpsI GAGTC 2 cut(s) 481, 494
PscI ACATGT 1 cut(s) 762
PsiI TTATAA 2 cut(s) 1050, 1188
Psp6I CCWGG 2 cut(s) 1019, 1161
PspFI CCCAGC 3 cut(s) 796, 970, 1289
PspGI CCWGG 2 cut(s) 1019, 1161
PspN4I GGNNCC 3 cut(s) 143, 184, 1018
PspPI GGNCC 5 cut(s) 182, 224, 291, 637, 1016
PstNI CAGNNNCTG 1 cut(s) 1344
PsuI RGATCY 1 cut(s) 379
PvuII CAGCTG 2 cut(s) 288, 800
RruI TCGCGA 1 cut(s) 741
RsaI GTAC 3 cut(s) 434, 767, 774
RsaNI GTAC 3 cut(s) 433, 766, 773
SaqAI TTAA 4 cut(s) 276, 426, 857, 936
SatI GCNGC 4 cut(s) 15, 190, 844, 1342
Sau3AI GATC 7 cut(s) 154, 379, 649, 684, 1071, 1217, 1261
Sau96I GGNCC 5 cut(s) 182, 224, 291, 637, 1016
SchI GAGTC 2 cut(s) 482, 495
ScrFI CCNGG 6 cut(s) 48, 186, 376, 469, 1021, 1163
SduI GDGCHC 3 cut(s) 29, 146, 1296
SfcI CTRYAG 1 cut(s) 1336
SinI GGWCC 3 cut(s) 182, 291, 1016
SmlI CTYRAG 3 cut(s) 557, 776, 856
SmoI CTYRAG 3 cut(s) 557, 776, 856
SpeI ACTAGT 1 cut(s) 1172
Sse9I AATT 5 cut(s) 6, 232, 987, 1025, 1051
SsiI CCGC 2 cut(s) 147, 1342
SspMI CTAG 4 cut(s) 195, 705, 872, 1173
StyD4I CCNGG 6 cut(s) 46, 184, 374, 467, 1019, 1161
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 3 cut(s) 312, 1279, 1300
TaiI ACGT 1 cut(s) 495
TaqI TCGA 1 cut(s) 1329
TasI AATT 5 cut(s) 6, 232, 987, 1025, 1051
TatI WGTACW 1 cut(s) 772
TauI GCSGC 1 cut(s) 1344
TfiI GAWTC 4 cut(s) 109, 207, 320, 582
Tru1I TTAA 4 cut(s) 276, 426, 857, 936
Tru9I TTAA 4 cut(s) 276, 426, 857, 936
TscAI CASTG 2 cut(s) 1129, 1282
TseFI GTSAC 1 cut(s) 915
TseI GCWGC 3 cut(s) 14, 189, 843
Tsp45I GTSAC 1 cut(s) 915
TspDTI ATGAA 3 cut(s) 957, 980, 1269
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 2 cut(s) 1129, 1282
Vha464I CTTAAG 1 cut(s) 856
VpaK11BI GGWCC 3 cut(s) 182, 291, 1016
XceI RCATGY 1 cut(s) 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XmiI GTMKAC 4 cut(s) 213, 489, 526, 1151
XmnI GAANNNNTTC 3 cut(s) 113, 808, 987
XspI CTAG 4 cut(s) 195, 705, 872, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.