Rh1BG121900

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0018671
Physical Location & Seq
Forward (+)
20668989 .. 20670104
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG121900.1

Sequence Viewer

Length: 798 bp
ATGGCAGTGGCGACACTGGGAGCGGCGTTCCAAGAGATCTGCGATCAAGTCACCCAAGCGATGGAGAAAAACACAAATAAAAAGCTGAAATCCGTCCTGGGTTTGTTAGAGGACACGCTCAATTCCTTGAAAGATGAAATCATCCAGCCCATTCATCAAGGCCACCAGATTTTCGACGATCGAAACGACAAGATCGAGTCCATCGTCGTCGAATTGCACGAGGGAGCGAAGCTAATTGCGAAAGTCTCCAAGCTCCGATCATGGAACTATTTCTGGCTCGACGAGGACGGCTACATGCGGAGGCTTCTTCAATTGGATGGGTACTTATTTCATCTGCTGCATACGACTCTGTATAGGCCGTTATCTGACGCTTATAAGAAGGCCGTGGAGGGCGGAGCGGTGCGGTTGACGGCGGAGCGGGGAAGTGGTGAAGAGGAAGAGGCTGTGTTCCGGAGGCTGTTTGCGGCGTCGATAGAGGCGGAGGAGAAGAGGAAGGCGCCCCAGTCGTTTAGGTTCCTGGTTTTGAATATACTCAGCAAAATATACGCGTTGCTGCCATTTTTGATGCACAACAATCCTAAGCTCCTCACCTCCTCTTTCAGAGCGGAGATGGAGAAGGGGTTGGAGGTGGTTCGGAGGAGCACCGGGGTTGGTAACAAGTGGCGGCTACGCAAGACCAATAAGTACGACAGTCGTACCTTTAGTGGGTTAGTGCGGCTGGATAAGTGTCTGACTCAGTGTCTACATGGACCTGTCCGTAAAGCAATCCAGCACGCATTGGATTTATCATCTAGTTAG

Protein Analysis

265

Amino Acids

30.23

Weight (kDa)

9.23

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 114 2.2e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018671)

Species Orthologous Gene IDs
rosa_laevigata RLG00000029295 RLG00000029491 RLG00000029777
rosa_multiflora Rmu_sc0002160.1_g000004 Rmu_sc0008098.1_g000001
rosa_roxburghii Rroxscaffold_4G00314680
rosa_rugosa Rorug01G0132300.1
rosa_samantha Rh1AG152800 Rh1BG121900 Rh1CG099200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AccB1I GGYRCC 1 cut(s) 496
AccB7I CCANNNNNTGG 1 cut(s) 61
AccBSI CCGCTC 4 cut(s) 23, 398, 418, 605
AccI GTMKAC 1 cut(s) 742
AccII CGCG 1 cut(s) 548
AccIII TCCGGA 1 cut(s) 450
AcyI GRCGYC 2 cut(s) 467, 497
AfaI GTAC 3 cut(s) 323, 686, 697
AfiI CCNNNNNNNGG 2 cut(s) 61, 705
AflIII ACRYGT 1 cut(s) 546
AgsI TTSAA 3 cut(s) 130, 311, 526
AhdI GACNNNNNGTC 1 cut(s) 738
AjnI CCWGG 2 cut(s) 96, 516
AluBI AGCT 4 cut(s) 85, 232, 253, 583
AluI AGCT 4 cut(s) 85, 232, 253, 583
Alw21I GWGCWC 1 cut(s) 644
Alw26I GTCTC 1 cut(s) 250
Aor13HI TCCGGA 1 cut(s) 450
AoxI GGCC 3 cut(s) 160, 356, 381
ApeKI GCWGC 2 cut(s) 337, 553
Asp700I GAANNNNTTC 1 cut(s) 269
AspLEI GCGC 1 cut(s) 499
AspS9I GGNCC 1 cut(s) 749
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 3 cut(s) 43, 440, 580
AvaII GGWCC 1 cut(s) 749
BaeI ACNNNNGTAYC 2 cut(s) 679, 712
BanI GGYRCC 1 cut(s) 496
BauI CACGAG 1 cut(s) 218
Bbv12I GWGCWC 1 cut(s) 644
BbvI GCAGC 2 cut(s) 324, 540
BccI CCATC 4 cut(s) 55, 209, 311, 604
BceAI ACGGC 4 cut(s) 304, 343, 368, 426
BciT130I CCWGG 2 cut(s) 98, 518
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 1 cut(s) 250
BfaI CTAG 1 cut(s) 792
BfoI RGCGCY 1 cut(s) 500
BglII AGATCT 1 cut(s) 36
BisI GCNGC 6 cut(s) 24, 338, 465, 554, 665, 716
BlsI GCNGC 6 cut(s) 25, 339, 466, 555, 666, 717
Bme1390I CCNGG 3 cut(s) 98, 518, 646
Bme18I GGWCC 1 cut(s) 749
BmeRI GACNNNNNGTC 1 cut(s) 738
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 2 cut(s) 498, 515
BmrFI CCNGG 3 cut(s) 98, 518, 646
BmrI ACTGGG 2 cut(s) 26, 496
BmsI GCATC 1 cut(s) 555
BmuI ACTGGG 2 cut(s) 26, 496
Bpu10I CCTNAGC 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 646
BsaHI GRCGYC 2 cut(s) 467, 497
BsaJI CCNNGG 3 cut(s) 97, 384, 645
BsaWI WCCGGW 1 cut(s) 450
BsaXI ACNNNNNCTCC 2 cut(s) 605, 635
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 705
Bse1I ACTGG 2 cut(s) 21, 502
BseAI TCCGGA 1 cut(s) 450
BseBI CCWGG 2 cut(s) 98, 518
BseDI CCNNGG 3 cut(s) 97, 384, 645
BseGI GGATG 2 cut(s) 141, 322
BseLI CCNNNNNNNGG 2 cut(s) 61, 705
BseMII CTCAG 2 cut(s) 547, 749
BseNI ACTGG 2 cut(s) 21, 502
BseRI GAGGAG 4 cut(s) 497, 575, 583, 652
BseXI GCAGC 2 cut(s) 324, 540
Bsh1236I CGCG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 3 cut(s) 162, 358, 383
BshNI GGYRCC 1 cut(s) 496
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 644
BsiSI CCGG 2 cut(s) 451, 645
BslI CCNNNNNNNGG 2 cut(s) 61, 705
BsmAI GTCTC 1 cut(s) 250
BsnI GGCC 3 cut(s) 162, 358, 383
Bsp1286I GDGCHC 1 cut(s) 644
Bsp13I TCCGGA 1 cut(s) 450
Bsp143I GATC 5 cut(s) 36, 43, 178, 192, 257
BspANI GGCC 3 cut(s) 162, 358, 383
BspCNI CTCAG 2 cut(s) 546, 748
BspEI TCCGGA 1 cut(s) 450
BspFNI CGCG 1 cut(s) 548
BspLI GGNNCC 2 cut(s) 498, 515
BspT107I GGYRCC 1 cut(s) 496
BsrBI CCGCTC 4 cut(s) 23, 398, 418, 605
BsrI ACTGG 2 cut(s) 21, 502
BssECI CCNNGG 3 cut(s) 97, 384, 645
BssMI GATC 5 cut(s) 36, 43, 178, 192, 257
BssNI GRCGYC 2 cut(s) 467, 497
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 2 cut(s) 98, 518
Bst4CI ACNGT 1 cut(s) 692
Bst6I CTCTTC 3 cut(s) 426, 432, 482
BstACI GRCGYC 2 cut(s) 467, 497
BstC8I GCNNGC 1 cut(s) 774
BstDEI CTNAG 3 cut(s) 533, 579, 735
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 141, 322
BstFNI CGCG 1 cut(s) 548
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 1 cut(s) 499
BstKTI GATC 5 cut(s) 39, 46, 181, 195, 260
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 5 cut(s) 36, 43, 178, 192, 257
BstMCI CGRYCG 1 cut(s) 181
BstNI CCWGG 2 cut(s) 98, 518
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 3 cut(s) 96, 516, 644
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 2 cut(s) 324, 540
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BsuRI GGCC 3 cut(s) 162, 358, 383
BtgI CCRYGG 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 74
BtsCI GGATG 2 cut(s) 141, 322
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 3 cut(s) 12, 14, 743
Cac8I GCNNGC 1 cut(s) 774
CfoI GCGC 1 cut(s) 499
Cfr13I GGNCC 1 cut(s) 749
CseI GACGC 2 cut(s) 377, 456
Csp6I GTAC 3 cut(s) 322, 685, 696
CviAII CATG 3 cut(s) 261, 295, 746
CviQI GTAC 3 cut(s) 322, 685, 696
DdeI CTNAG 3 cut(s) 533, 579, 735
DinI GGCGCC 1 cut(s) 498
DpnI GATC 5 cut(s) 38, 45, 180, 194, 259
DpnII GATC 5 cut(s) 36, 43, 178, 192, 257
DriI GACNNNNNGTC 1 cut(s) 738
Eam1104I CTCTTC 3 cut(s) 426, 432, 482
Eam1105I GACNNNNNGTC 1 cut(s) 738
EarI CTCTTC 3 cut(s) 426, 432, 482
EciI GGCGGA 3 cut(s) 408, 428, 494
Eco47I GGWCC 1 cut(s) 749
EcoRII CCWGG 2 cut(s) 96, 516
EgeI GGCGCC 1 cut(s) 498
EheI GGCGCC 1 cut(s) 498
FaeI CATG 3 cut(s) 264, 298, 749
FaiI YATR 8 cut(s) 262, 296, 342, 354, 375, 530, 544, 747
FatI CATG 3 cut(s) 260, 294, 745
FauI CCCGC 1 cut(s) 411
FblI GTMKAC 1 cut(s) 742
Fnu4HI GCNGC 6 cut(s) 24, 338, 465, 554, 665, 716
FokI GGATG 2 cut(s) 128, 329
Fsp4HI GCNGC 6 cut(s) 24, 338, 465, 554, 665, 716
FspBI CTAG 1 cut(s) 792
GlaI GCGC 1 cut(s) 498
GluI GCNGC 6 cut(s) 24, 338, 465, 554, 665, 716
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 3 cut(s) 162, 358, 383
HapII CCGG 2 cut(s) 451, 645
HgaI GACGC 2 cut(s) 377, 456
HhaI GCGC 1 cut(s) 499
Hin1I GRCGYC 2 cut(s) 467, 497
Hin1II CATG 3 cut(s) 264, 298, 749
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 3 cut(s) 197, 346, 733
HpaII CCGG 2 cut(s) 451, 645
HphI GGTGA 3 cut(s) 43, 440, 580
Hpy166II GTNNAC 2 cut(s) 408, 743
Hpy188I TCNGA 5 cut(s) 257, 367, 602, 636, 732
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 2 cut(s) 408, 743
Hpy99I CGWCG 5 cut(s) 179, 209, 212, 284, 472
HpyAV CCTTC 3 cut(s) 373, 487, 610
HpyCH4III ACNGT 1 cut(s) 692
HpyCH4V TGCA 3 cut(s) 217, 340, 568
HpyF3I CTNAG 3 cut(s) 533, 579, 735
Hsp92I GRCGYC 2 cut(s) 467, 497
Hsp92II CATG 3 cut(s) 264, 298, 749
HspAI GCGC 1 cut(s) 497
KasI GGCGCC 1 cut(s) 496
Kpn2I TCCGGA 1 cut(s) 450
Kzo9I GATC 5 cut(s) 36, 43, 178, 192, 257
LmnI GCTCC 7 cut(s) 20, 224, 258, 395, 415, 588, 639
Lsp1109I GCAGC 2 cut(s) 324, 540
LweI GCATC 1 cut(s) 555
MaeI CTAG 1 cut(s) 792
MaeIII GTNAC 2 cut(s) 49, 653
MalI GATC 5 cut(s) 38, 45, 180, 194, 259
MbiI CCGCTC 4 cut(s) 23, 398, 418, 605
MboI GATC 5 cut(s) 36, 43, 178, 192, 257
MboII GAAGA 4 cut(s) 299, 443, 449, 499
MfeI CAATTG 1 cut(s) 311
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 644
MluCI AATT 4 cut(s) 121, 212, 234, 311
MluI ACGCGT 1 cut(s) 546
Mly113I GGCGCC 1 cut(s) 497
MlyI GAGTC 3 cut(s) 206, 340, 727
MmeI TCCRAC 1 cut(s) 603
MroI TCCGGA 1 cut(s) 450
MroXI GAANNNNTTC 1 cut(s) 269
MspI CCGG 2 cut(s) 451, 645
MspR9I CCNGG 3 cut(s) 98, 518, 646
MunI CAATTG 1 cut(s) 311
MvaI CCWGG 2 cut(s) 98, 518
MvnI CGCG 1 cut(s) 548
NarI GGCGCC 1 cut(s) 497
NciI CCSGG 1 cut(s) 646
NdeII GATC 5 cut(s) 36, 43, 178, 192, 257
NlaIII CATG 3 cut(s) 264, 298, 749
NlaIV GGNNCC 2 cut(s) 498, 515
NmuCI GTSAC 1 cut(s) 49
NspI RCATGY 1 cut(s) 298
PcsI WCGNNNNNNNCGW 4 cut(s) 183, 192, 201, 216
PdmI GAANNNNTTC 1 cut(s) 269
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 6 cut(s) 25, 339, 466, 555, 666, 717
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 3 cut(s) 205, 340, 727
PluTI GGCGCC 1 cut(s) 500
PpsI GAGTC 3 cut(s) 205, 340, 727
PsiI TTATAA 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 96, 516
PspGI CCWGG 2 cut(s) 96, 516
PspN4I GGNNCC 2 cut(s) 498, 515
PspPI GGNCC 1 cut(s) 749
PsuI RGATCY 1 cut(s) 36
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 3 cut(s) 323, 686, 697
RsaNI GTAC 3 cut(s) 322, 685, 696
SatI GCNGC 6 cut(s) 24, 338, 465, 554, 665, 716
Sau3AI GATC 5 cut(s) 36, 43, 178, 192, 257
Sau96I GGNCC 1 cut(s) 749
SchI GAGTC 3 cut(s) 206, 340, 727
ScrFI CCNGG 3 cut(s) 98, 518, 646
SduI GDGCHC 1 cut(s) 644
SetI ASST 9 cut(s) 87, 234, 255, 515, 585, 593, 630, 701, 754
SfaNI GCATC 1 cut(s) 555
SfoI GGCGCC 1 cut(s) 498
SinI GGWCC 1 cut(s) 749
Sse9I AATT 4 cut(s) 121, 212, 234, 311
SspDI GGCGCC 1 cut(s) 496
SspMI CTAG 1 cut(s) 792
StyD4I CCNGG 3 cut(s) 96, 516, 644
TaaI ACNGT 1 cut(s) 692
TaqI TCGA 6 cut(s) 174, 181, 195, 210, 279, 470
TasI AATT 4 cut(s) 121, 212, 234, 311
TauI GCSGC 4 cut(s) 26, 467, 667, 718
TscAI CASTG 3 cut(s) 12, 21, 743
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 2 cut(s) 337, 553
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 3 cut(s) 143, 150, 320
TspGWI ACGGA 2 cut(s) 82, 746
TspRI CASTG 3 cut(s) 12, 21, 743
Van91I CCANNNNNTGG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 749
XceI RCATGY 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 742
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.