Rh1BG197200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010020
Physical Location & Seq
Reverse (-)
31395108 .. 31398294
3187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG197200.1

Sequence Viewer

Length: 1257 bp
ATGGCCACCTCTGTTTCCAATGCGGTTTGCTTTCGCTATCCCCACCCACCAAACCCAACACTCAACGAACCCAGAAAAACCCACCTCCCCCAATTCCCCGGCAACACTTTCCGGGCAAAACCCATGAATCTTTGCTCTTCCAGCCACTGTTTTCAGCCACCATTGATGGCTCTAAGTGTAGAAAAGACAGCAATGAATGAAAATACAGAAGGGAAGCCAATGTTTAAGTGGGGTGTGATAGGTCCTGATCTTACAGAAGCCCAGCAAGAAGCTATAGATGAACTCCCTCCCAAGATGAGCAAGCGTTGCCAGGCTTTGATGAAGCAGATTATTTGCTTCTCTCCTGAGAAAGGGAGTCTTTGTGAGGTGTTGGATGCTTGGGTTAGCATTATGAAGCCTTGCAGAGCTGACTGGCTTGCGGTTCTTAAAGAACTGAGGATTAAAGATCATCCACTTTATCTTCAGGTGGCAGAAATTGCTGTACTAGATGAATCTTTTGAAGCCAATGTGCGTGACTATACGAAAATAATCCATGGTTATGGGAAGCAAAACCGAATTGAGGATGCTGAAAACACCCTTTCAAACATGAAGTCTAGAGGCTTCGTCTGTGATCAGGTAACGCTAACTGCCATGATTGACATGTATAGCAAGGCTGGACATCTTAAGCTGGCTGAGGATACTTTTGAAGAAATTAAGCTCCTTGGACAGCAATTAGATAAAAGATCATATGGCTCAATGATCATGGCCTACATTAGAGCTGGAATGCCTGATCAGGGAGAGAATTTACTCATAGAAATGGATGCCCAAGAAATTTATGCTGGAAGTGAAGTTTACAAGGCATTGTTAAGAGCATACTCAATGATTGGTGATACTGAAGGAGCTCAAAGGGTATTCAATGCAGTTCAGCTAGCTGGTATTTCTCCAGATGCTAAGATATGTGGACTTCTCATAAATGCATATGGTACATCAGGTCAAAGTCAAAAGGCACGTGCTGCGTTTGAAAATATGAGGAAGGCTGGTCTTAAACCCAGTGATAAATGCATAGCTCTAATGTTGGCTGCATACGAAACGGAGAATAAGCTTCAAATGGCATTGAAGTTTCTGATGGGGTTGGAGAGAGAAGGTATCATGGTTGGGAAAGAAGCTGCAGAAACACTGGCTGGGTGGTTTAGAAAACTAGGAGTTGTGGAAGAGGTGGATATGGTCTTGAGAGAATTTGCAGCAACAGAAGCTAATTCTGGAATTCCTGCTTCCTAG

Protein Analysis

418

Amino Acids

46.39

Weight (kDa)

5.84

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 173 - 213 3.6e-09 PPR repeat family
PPR_long PF17177 182 - 292 9.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 204 - 250 9.2e-07 PPR repeat family
PPR_long PF17177 262 - 391 3.1e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 23, 419
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 781, 810, 1214, 1242
AcuI CTGAAG 2 cut(s) 446, 894
AcvI CACGTG 1 cut(s) 989
AfaI GTAC 2 cut(s) 483, 964
AfiI CCNNNNNNNGG 3 cut(s) 350, 559, 773
AflII CTTAAG 1 cut(s) 662
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 7 cut(s) 500, 582, 686, 895, 1001, 1085, 1096
AjnI CCWGG 1 cut(s) 309
Alw21I GWGCWC 1 cut(s) 883
AlwNI CAGNNNCTG 1 cut(s) 147
AoxI GGCC 2 cut(s) 3, 744
ApeKI GCWGC 4 cut(s) 992, 1058, 1145, 1220
ApoI RAATTY 4 cut(s) 781, 810, 1214, 1242
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 2 cut(s) 99, 113
AsuHPI GGTGA 1 cut(s) 878
AsuNHI GCTAGC 1 cut(s) 907
AvaII GGWCC 1 cut(s) 242
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 883
BbrPI CACGTG 1 cut(s) 989
Bbv12I GWGCWC 1 cut(s) 883
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 4 cut(s) 979, 1045, 1132, 1232
BccI CCATC 2 cut(s) 160, 1099
BciT130I CCWGG 1 cut(s) 311
BciVI GTATCC 1 cut(s) 670
BclI TGATCA 3 cut(s) 610, 738, 769
BcnI CCSGG 2 cut(s) 99, 113
BfaI CTAG 5 cut(s) 485, 594, 908, 1178, 1255
BfmI CTRYAG 2 cut(s) 273, 1146
BfrI CTTAAG 1 cut(s) 662
BfuI GTATCC 1 cut(s) 670
BisI GCNGC 4 cut(s) 993, 1059, 1146, 1221
BlsI GCNGC 4 cut(s) 994, 1060, 1147, 1222
Bme1390I CCNGG 3 cut(s) 99, 113, 311
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 242
BmrFI CCNGG 3 cut(s) 99, 113, 311
BmrI ACTGGG 1 cut(s) 1023
BmsI GCATC 4 cut(s) 364, 553, 790, 916
BmtI GCTAGC 1 cut(s) 911
BmuI ACTGGG 1 cut(s) 1023
BpmI CTGGAG 1 cut(s) 906
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 1228
BpuMI CCSGG 2 cut(s) 99, 113
BsaAI YACGTR 1 cut(s) 989
BsaJI CCNNGG 3 cut(s) 97, 532, 700
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 559, 773
Bse1I ACTGG 3 cut(s) 416, 1029, 1161
Bse3DI GCAATG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 3 cut(s) 97, 532, 700
BseGI GGATG 4 cut(s) 379, 448, 568, 805
BseLI CCNNNNNNNGG 3 cut(s) 350, 559, 773
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 3 cut(s) 336, 425, 663
BseNI ACTGG 3 cut(s) 416, 1029, 1161
BseXI GCAGC 4 cut(s) 979, 1045, 1132, 1232
BseYI CCCAGC 2 cut(s) 261, 1160
BshFI GGCC 2 cut(s) 5, 746
BsiHKAI GWGCWC 1 cut(s) 883
BsiSI CCGG 2 cut(s) 99, 112
BslI CCNNNNNNNGG 3 cut(s) 350, 559, 773
BsmI GAATGC 1 cut(s) 768
BsnI GGCC 2 cut(s) 5, 746
Bsp1286I GDGCHC 1 cut(s) 883
Bsp143I GATC 6 cut(s) 247, 445, 610, 722, 738, 769
Bsp19I CCATGG 1 cut(s) 532
BspACI CCGC 2 cut(s) 23, 419
BspANI GGCC 2 cut(s) 5, 746
BspCNI CTCAG 3 cut(s) 337, 426, 664
BspMAI CTGCAG 1 cut(s) 1150
BspOI GCTAGC 1 cut(s) 911
BspQI GCTCTTC 1 cut(s) 142
BspTI CTTAAG 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 198
BsrI ACTGG 3 cut(s) 416, 1029, 1161
BssECI CCNNGG 3 cut(s) 97, 532, 700
BssMI GATC 6 cut(s) 247, 445, 610, 722, 738, 769
BssT1I CCWWGG 2 cut(s) 532, 700
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 1 cut(s) 149
Bst6I CTCTTC 2 cut(s) 142, 1185
BstAFI CTTAAG 1 cut(s) 662
BstAPI GCANNNNNTGC 3 cut(s) 306, 476, 992
BstBAI YACGTR 1 cut(s) 989
BstC8I GCNNGC 4 cut(s) 302, 417, 669, 909
BstDEI CTNAG 5 cut(s) 173, 345, 434, 672, 930
BstDSI CCRYGG 1 cut(s) 532
BstENI CCTNNNNNAGG 1 cut(s) 348
BstF5I GGATG 4 cut(s) 379, 448, 568, 805
BstKTI GATC 6 cut(s) 250, 448, 613, 725, 741, 772
BstMBI GATC 6 cut(s) 247, 445, 610, 722, 738, 769
BstMWI GCNNNNNNNGC 5 cut(s) 141, 306, 476, 992, 1229
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 3 cut(s) 97, 111, 309
BstSFI CTRYAG 2 cut(s) 273, 1146
BstV1I GCAGC 4 cut(s) 979, 1045, 1132, 1232
BstXI CCANNNNNNTGG 1 cut(s) 539
BsuI GTATCC 1 cut(s) 670
BsuRI GGCC 2 cut(s) 5, 746
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 4 cut(s) 379, 448, 568, 805
BtsIMutI CAGTG 3 cut(s) 145, 1036, 1154
Cac8I GCNNGC 4 cut(s) 302, 417, 669, 909
CaiI CAGNNNCTG 1 cut(s) 147
Cfr13I GGNCC 1 cut(s) 242
Csp6I GTAC 2 cut(s) 482, 963
CviAII CATG 7 cut(s) 124, 533, 586, 631, 640, 742, 1129
CviQI GTAC 2 cut(s) 482, 963
DdeI CTNAG 5 cut(s) 173, 345, 434, 672, 930
DpnI GATC 6 cut(s) 249, 447, 612, 724, 740, 771
DpnII GATC 6 cut(s) 247, 445, 610, 722, 738, 769
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 142, 1185
EarI CTCTTC 2 cut(s) 142, 1185
Ecl136II GAGCTC 1 cut(s) 881
Eco130I CCWWGG 2 cut(s) 532, 700
Eco24I GRGCYC 1 cut(s) 883
Eco47I GGWCC 1 cut(s) 242
Eco53kI GAGCTC 1 cut(s) 881
Eco57I CTGAAG 2 cut(s) 446, 894
Eco72I CACGTG 1 cut(s) 989
EcoICRI GAGCTC 1 cut(s) 881
EcoNI CCTNNNNNAGG 1 cut(s) 348
EcoO109I RGGNCCY 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 1242
EcoRII CCWGG 1 cut(s) 309
EcoT14I CCWWGG 2 cut(s) 532, 700
EcoT22I ATGCAT 2 cut(s) 958, 1043
EcoT38I GRGCYC 1 cut(s) 883
ErhI CCWWGG 2 cut(s) 532, 700
FaeI CATG 7 cut(s) 127, 536, 589, 634, 643, 745, 1132
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FatI CATG 7 cut(s) 123, 532, 585, 630, 639, 741, 1128
FauNDI CATATG 2 cut(s) 727, 958
FbaI TGATCA 3 cut(s) 610, 738, 769
Fnu4HI GCNGC 4 cut(s) 993, 1059, 1146, 1221
FokI GGATG 4 cut(s) 386, 435, 575, 812
FriOI GRGCYC 1 cut(s) 883
Fsp4HI GCNGC 4 cut(s) 993, 1059, 1146, 1221
FspBI CTAG 5 cut(s) 485, 594, 908, 1178, 1255
GluI GCNGC 4 cut(s) 993, 1059, 1146, 1221
GsaI CCCAGC 2 cut(s) 265, 1164
GsuI CTGGAG 1 cut(s) 906
HaeIII GGCC 2 cut(s) 5, 746
HapII CCGG 2 cut(s) 99, 112
Hin1II CATG 7 cut(s) 127, 536, 589, 634, 643, 745, 1132
HindIII AAGCTT 1 cut(s) 1079
HinfI GANTC 3 cut(s) 127, 355, 491
HpaII CCGG 2 cut(s) 99, 112
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 2 cut(s) 832, 941
Hpy188I TCNGA 1 cut(s) 1104
Hpy188III TCNNGA 6 cut(s) 245, 344, 594, 923, 1207, 1239
Hpy8I GTNNAC 2 cut(s) 832, 941
HpyAV CCTTC 4 cut(s) 203, 869, 1006, 1115
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4IV ACGT 1 cut(s) 988
HpyCH4V TGCA 7 cut(s) 402, 899, 956, 1041, 1061, 1148, 1220
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 306, 476, 992, 1229
HpyF3I CTNAG 5 cut(s) 173, 345, 434, 672, 930
HpySE526I ACGT 1 cut(s) 988
Hsp92II CATG 7 cut(s) 127, 536, 589, 634, 643, 745, 1132
Ksp22I TGATCA 3 cut(s) 610, 738, 769
Kzo9I GATC 6 cut(s) 247, 445, 610, 722, 738, 769
LguI GCTCTTC 1 cut(s) 142
LmnI GCTCC 2 cut(s) 702, 878
Lsp1109I GCAGC 4 cut(s) 979, 1045, 1132, 1232
LweI GCATC 4 cut(s) 364, 553, 790, 916
MaeI CTAG 5 cut(s) 485, 594, 908, 1178, 1255
MaeII ACGT 1 cut(s) 988
MaeIII GTNAC 2 cut(s) 512, 616
MalI GATC 6 cut(s) 249, 447, 612, 724, 740, 771
MboI GATC 6 cut(s) 247, 445, 610, 722, 738, 769
MboII GAAGA 4 cut(s) 129, 452, 698, 1202
MhlI GDGCHC 1 cut(s) 883
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 2 cut(s) 351, 1092
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 958, 1043
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 225, 426, 441, 663, 693, 845, 1023
MslI CAYNNNNRTG 2 cut(s) 537, 794
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 2 cut(s) 99, 112
MspR9I CCNGG 3 cut(s) 99, 113, 311
Mva1269I GAATGC 1 cut(s) 768
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 5 cut(s) 141, 306, 476, 992, 1229
NciI CCSGG 2 cut(s) 99, 113
NcoI CCATGG 1 cut(s) 532
NdeI CATATG 2 cut(s) 727, 958
NdeII GATC 6 cut(s) 247, 445, 610, 722, 738, 769
NheI GCTAGC 1 cut(s) 907
NlaIII CATG 7 cut(s) 127, 536, 589, 634, 643, 745, 1132
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 2 cut(s) 958, 1043
NspI RCATGY 1 cut(s) 643
PciI ACATGT 1 cut(s) 639
PciSI GCTCTTC 1 cut(s) 142
PctI GAATGC 1 cut(s) 768
PfeI GAWTC 2 cut(s) 127, 491
PkrI GCNGC 4 cut(s) 994, 1060, 1147, 1222
PleI GAGTC 1 cut(s) 363
PmaCI CACGTG 1 cut(s) 989
PmlI CACGTG 1 cut(s) 989
PpsI GAGTC 1 cut(s) 363
Ppu21I YACGTR 1 cut(s) 989
PpuMI RGGWCCY 1 cut(s) 242
PscI ACATGT 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 883
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 309
PspCI CACGTG 1 cut(s) 989
PspFI CCCAGC 2 cut(s) 261, 1160
PspGI CCWGG 1 cut(s) 309
PspPI GGNCC 1 cut(s) 242
PspPPI RGGWCCY 1 cut(s) 242
PstI CTGCAG 1 cut(s) 1150
PstNI CAGNNNCTG 1 cut(s) 147
RsaI GTAC 2 cut(s) 483, 964
RsaNI GTAC 2 cut(s) 482, 963
RseI CAYNNNNRTG 2 cut(s) 537, 794
SacI GAGCTC 1 cut(s) 883
SapI GCTCTTC 1 cut(s) 142
SaqAI TTAA 7 cut(s) 225, 426, 441, 663, 693, 845, 1023
SatI GCNGC 4 cut(s) 993, 1059, 1146, 1221
Sau3AI GATC 6 cut(s) 247, 445, 610, 722, 738, 769
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 3 cut(s) 99, 113, 311
SduI GDGCHC 1 cut(s) 883
SfaNI GCATC 4 cut(s) 364, 553, 790, 916
SfcI CTRYAG 2 cut(s) 273, 1146
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 2 cut(s) 537, 794
SmlI CTYRAG 2 cut(s) 662, 1207
SmoI CTYRAG 2 cut(s) 662, 1207
SsiI CCGC 2 cut(s) 23, 419
SspMI CTAG 5 cut(s) 485, 594, 908, 1178, 1255
SstI GAGCTC 1 cut(s) 883
StyD4I CCNGG 3 cut(s) 97, 111, 309
StyI CCWWGG 2 cut(s) 532, 700
TaaI ACNGT 1 cut(s) 149
TaiI ACGT 1 cut(s) 991
TatI WGTACW 1 cut(s) 481
TfiI GAWTC 2 cut(s) 127, 491
Tru1I TTAA 7 cut(s) 225, 426, 441, 663, 693, 845, 1023
Tru9I TTAA 7 cut(s) 225, 426, 441, 663, 693, 845, 1023
TscAI CASTG 3 cut(s) 152, 1036, 1161
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 4 cut(s) 992, 1058, 1145, 1220
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 8 cut(s) 140, 209, 213, 294, 335, 407, 504, 602
TspGWI ACGGA 1 cut(s) 1085
TspRI CASTG 3 cut(s) 152, 1036, 1161
Vha464I CTTAAG 1 cut(s) 662
VpaK11BI GGWCC 1 cut(s) 242
XagI CCTNNNNNAGG 1 cut(s) 348
XapI RAATTY 4 cut(s) 781, 810, 1214, 1242
XbaI TCTAGA 1 cut(s) 593
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XspI CTAG 5 cut(s) 485, 594, 908, 1178, 1255
Zsp2I ATGCAT 2 cut(s) 958, 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.