Rh1CG218400

Deoxyhypusine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0019081
Physical Location & Seq
Forward (+)
46885214 .. 46893801
8588 bp
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UTR
Exon/CDS
Intron
Rh1CG218400.1

Sequence Viewer

Length: 810 bp
ATGGGCACGCCGAACCACCACAAAGGCTTCAAGATCCTCAAGAAGATAACCGCCAAATCCATGACACGACCCTACCCGAAGGAGCCCCAGTTTGCCACGCGCTCTTCTTTGCAGCCCAGCTCCCGCCGCAGATTTCGCTTGAGAAAAAGACTATCGTCCTCAATCTCAACGAGATCATGGTCCACTCGCAAGCCGGACCGCCGCCTGAGAAGTTTGACTCTGTCGTTTTTTGGGATTTCGAGCGGGCTCGGCGAGGAGGAGAGAGAGAGTTGGGCGGAGTCGAATTGTTTGGAATTTCCGTCTTCAAAGAGGACCTGGAAGAAGCCGGAGAAGGCAAAGAAGGATTTCACAGTTCCGATGACACCTTTCAACTATTTCCTCACACTTCTCCCAACGAATTTAATGCTCACCACTGGTTTCCAAGCTACCAATCTCGGAGAAGCCATCAAAGTCGTCAACCAAATGGTAGATTGGAGGCTGGCAGATGAGGTTGTAGCTGAAGATAGTGGTGAGGAGAAGAGGGATGGGAAGTTTAGAGAGTCGGTGAAGTGTAAAGTGTTTTTGGGTTTCACTTCGAATTTGGTTTCATCTGGGCTTAGAGATGTGTTTAGGTGCTTGGTGGACCGTCGCACCAATGACAGTACACGGAGACTTTCTTTGCCAATGGGTCATCGAAATGGCTCCAATCCTTCAGCCTATATGGGTGACATTTGCGACGGTCATCGTTTTTTACTTGAGGTCACAATTTTGGAATCGATTGTTCTGATTTTCATTAATTGCAAGATTTTGAGCTGTACGGGTCGAGATTGA

Protein Analysis

269

Amino Acids

30.73

Weight (kDa)

10.05

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DS PF01916 135 - 211 3.7e-13 Deoxyhypusine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019081)

Species Orthologous Gene IDs
rosa_laevigata RLG00000028398
rosa_multiflora Rmu_sc0001144.1_g000022
rosa_roxburghii Rroxscaffold_1G00034370 Rroxscaffold_4G00303700 Rroxscaffold_6G00397550 Rroxscaffold_7G00190850
rosa_rugosa Rorug02G0028200
rosa_samantha Rh1AG233800 Rh1CG218400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 243
AccII CGCG 1 cut(s) 100
AciI CCGC 7 cut(s) 51, 124, 127, 199, 202, 243, 275
AclWI GGATC 1 cut(s) 28
AcsI RAATTY 3 cut(s) 293, 397, 577
AcuI CTGAAG 2 cut(s) 519, 675
AfaI GTAC 2 cut(s) 643, 796
AgsI TTSAA 3 cut(s) 31, 306, 370
AjnI CCWGG 1 cut(s) 314
AluBI AGCT 4 cut(s) 120, 425, 497, 792
AluI AGCT 4 cut(s) 120, 425, 497, 792
Alw26I GTCTC 1 cut(s) 643
AlwI GGATC 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 112
ApoI RAATTY 3 cut(s) 293, 397, 577
AseI ATTAAT 1 cut(s) 774
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 4 cut(s) 180, 196, 312, 622
AsuHPI GGTGA 4 cut(s) 400, 521, 556, 716
AsuII TTCGAA 1 cut(s) 575
AvaII GGWCC 4 cut(s) 180, 196, 312, 622
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 2 cut(s) 87, 249
BbsI GAAGAC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 124
BccI CCATC 2 cut(s) 452, 518
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 643
BisI GCNGC 3 cut(s) 113, 127, 202
BlsI GCNGC 3 cut(s) 114, 128, 203
Bme1390I CCNGG 1 cut(s) 316
Bme18I GGWCC 4 cut(s) 180, 196, 312, 622
BmgT120I GGNCC 4 cut(s) 180, 196, 312, 622
BmiI GGNNCC 2 cut(s) 84, 682
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 82
BmuI ACTGGG 1 cut(s) 82
BoxI GACNNNNGTC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 575
BpuEI CTTGAG 3 cut(s) 23, 160, 755
Bsa29I ATCGAT 1 cut(s) 755
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bse1I ACTGG 2 cut(s) 88, 418
BseBI CCWGG 1 cut(s) 316
BseCI ATCGAT 1 cut(s) 755
BseGI GGATG 1 cut(s) 529
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 2 cut(s) 88, 418
BseRI GAGGAG 3 cut(s) 269, 272, 527
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 124
BseYI CCCAGC 1 cut(s) 116
Bsh1236I CGCG 1 cut(s) 100
BshVI ATCGAT 1 cut(s) 755
BsiSI CCGG 2 cut(s) 194, 326
BsmAI GTCTC 1 cut(s) 643
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 3 cut(s) 8, 87, 249
Bsp143I GATC 2 cut(s) 33, 173
BspACI CCGC 7 cut(s) 51, 124, 127, 199, 202, 243, 275
BspCNI CTCAG 1 cut(s) 198
BspDI ATCGAT 1 cut(s) 755
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 84, 682
BspPI GGATC 1 cut(s) 28
BspQI GCTCTTC 1 cut(s) 109
BspT104I TTCGAA 1 cut(s) 575
BsrBI CCGCTC 1 cut(s) 243
BsrI ACTGG 2 cut(s) 88, 418
BssMI GATC 2 cut(s) 33, 173
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 4 cut(s) 352, 626, 641, 719
Bst6I CTCTTC 2 cut(s) 109, 512
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 4 cut(s) 8, 191, 245, 480
BstDEI CTNAG 2 cut(s) 206, 596
BstF5I GGATG 1 cut(s) 529
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 2 cut(s) 36, 176
BstMAI GTCTC 1 cut(s) 643
BstMBI GATC 2 cut(s) 33, 173
BstMWI GCNNNNNNNGC 3 cut(s) 126, 135, 249
BstNI CCWGG 1 cut(s) 316
BstPAI GACNNNNGTC 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 314
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 1 cut(s) 124
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 755
BsuTUI ATCGAT 1 cut(s) 755
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 4 cut(s) 8, 191, 245, 480
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 4 cut(s) 180, 196, 312, 622
ClaI ATCGAT 1 cut(s) 755
CpoI CGGWCCG 1 cut(s) 196
Csp6I GTAC 2 cut(s) 642, 795
CspI CGGWCCG 1 cut(s) 196
CviAII CATG 2 cut(s) 61, 177
CviQI GTAC 2 cut(s) 642, 795
DdeI CTNAG 2 cut(s) 206, 596
DpnI GATC 2 cut(s) 35, 175
DpnII GATC 2 cut(s) 33, 173
Eam1104I CTCTTC 2 cut(s) 109, 512
EarI CTCTTC 2 cut(s) 109, 512
EciI GGCGGA 1 cut(s) 290
Eco24I GRGCYC 2 cut(s) 87, 249
Eco47I GGWCC 4 cut(s) 180, 196, 312, 622
Eco57I CTGAAG 2 cut(s) 519, 675
EcoO109I RGGNCCY 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 314
EcoT38I GRGCYC 2 cut(s) 87, 249
FaeI CATG 2 cut(s) 64, 180
FaiI YATR 4 cut(s) 62, 178, 699, 701
FatI CATG 2 cut(s) 60, 176
FauI CCCGC 2 cut(s) 131, 236
Fnu4HI GCNGC 3 cut(s) 113, 127, 202
FokI GGATG 1 cut(s) 536
FriOI GRGCYC 2 cut(s) 87, 249
Fsp4HI GCNGC 3 cut(s) 113, 127, 202
GlaI GCGC 1 cut(s) 101
GluI GCNGC 3 cut(s) 113, 127, 202
GsaI CCCAGC 1 cut(s) 120
HapII CCGG 2 cut(s) 194, 326
HhaI GCGC 1 cut(s) 102
Hin1II CATG 2 cut(s) 64, 180
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 4 cut(s) 217, 278, 539, 752
HpaII CCGG 2 cut(s) 194, 326
HphI GGTGA 4 cut(s) 400, 521, 556, 716
Hpy166II GTNNAC 4 cut(s) 183, 457, 622, 644
Hpy188I TCNGA 3 cut(s) 357, 437, 765
Hpy188III TCNNGA 3 cut(s) 31, 40, 803
Hpy8I GTNNAC 4 cut(s) 183, 457, 622, 644
Hpy99I CGWCG 2 cut(s) 630, 719
HpyAV CCTTC 4 cut(s) 73, 325, 334, 699
HpyCH4III ACNGT 4 cut(s) 352, 626, 641, 719
HpyCH4V TGCA 2 cut(s) 112, 780
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 135, 249
HpyF3I CTNAG 2 cut(s) 206, 596
Hsp92II CATG 2 cut(s) 64, 180
HspAI GCGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 33, 173
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 3 cut(s) 82, 125, 686
Lsp1109I GCAGC 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 704, 739
MalI GATC 2 cut(s) 35, 175
MbiI CCGCTC 1 cut(s) 243
MboI GATC 2 cut(s) 33, 173
MboII GAAGA 6 cut(s) 55, 96, 294, 331, 512, 529
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 3 cut(s) 8, 87, 249
MluCI AATT 6 cut(s) 283, 293, 397, 577, 744, 775
MlyI GAGTC 3 cut(s) 211, 287, 548
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 2 cut(s) 401, 774
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 2 cut(s) 194, 326
MspR9I CCNGG 1 cut(s) 316
MvaI CCWGG 1 cut(s) 316
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 3 cut(s) 126, 135, 249
NdeII GATC 2 cut(s) 33, 173
NlaIII CATG 2 cut(s) 64, 180
NlaIV GGNNCC 2 cut(s) 84, 682
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 2 cut(s) 704, 739
NspV TTCGAA 1 cut(s) 575
PciSI GCTCTTC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 1 cut(s) 752
PflFI GACNNNGTC 1 cut(s) 220
PkrI GCNGC 3 cut(s) 114, 128, 203
PleI GAGTC 3 cut(s) 211, 286, 547
PpsI GAGTC 3 cut(s) 211, 286, 547
PpuMI RGGWCCY 1 cut(s) 312
PshAI GACNNNNGTC 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 774
Psp5II RGGWCCY 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 116
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 84, 682
PspPI GGNCC 4 cut(s) 180, 196, 312, 622
PspPPI RGGWCCY 1 cut(s) 312
PsuI RGATCY 1 cut(s) 33
PsyI GACNNNGTC 1 cut(s) 220
RsaI GTAC 2 cut(s) 643, 796
RsaNI GTAC 2 cut(s) 642, 795
RseI CAYNNNNRTG 1 cut(s) 675
Rsr2I CGGWCCG 1 cut(s) 196
RsrII CGGWCCG 1 cut(s) 196
SapI GCTCTTC 1 cut(s) 109
SaqAI TTAA 2 cut(s) 401, 774
SatI GCNGC 3 cut(s) 113, 127, 202
Sau3AI GATC 2 cut(s) 33, 173
Sau96I GGNCC 4 cut(s) 180, 196, 312, 622
SchI GAGTC 3 cut(s) 211, 287, 548
ScrFI CCNGG 1 cut(s) 316
SduI GDGCHC 3 cut(s) 8, 87, 249
SetI ASST 9 cut(s) 122, 317, 367, 427, 492, 499, 614, 741, 794
SfuI TTCGAA 1 cut(s) 575
SinI GGWCC 4 cut(s) 180, 196, 312, 622
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 3 cut(s) 38, 139, 734
SmoI CTYRAG 3 cut(s) 38, 139, 734
Sse9I AATT 6 cut(s) 283, 293, 397, 577, 744, 775
SsiI CCGC 7 cut(s) 51, 124, 127, 199, 202, 243, 275
StyD4I CCNGG 1 cut(s) 314
TaaI ACNGT 4 cut(s) 352, 626, 641, 719
TaqI TCGA 6 cut(s) 239, 281, 575, 673, 755, 802
TasI AATT 6 cut(s) 283, 293, 397, 577, 744, 775
TatI WGTACW 1 cut(s) 641
TauI GCSGC 2 cut(s) 129, 204
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 2 cut(s) 401, 774
Tru9I TTAA 2 cut(s) 401, 774
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 2 cut(s) 704, 739
TseI GCWGC 1 cut(s) 112
Tsp45I GTSAC 2 cut(s) 704, 739
TspDTI ATGAA 2 cut(s) 576, 760
TspGWI ACGGA 2 cut(s) 288, 661
TspRI CASTG 1 cut(s) 418
Tth111I GACNNNGTC 1 cut(s) 220
VpaK11BI GGWCC 4 cut(s) 180, 196, 312, 622
VspI ATTAAT 1 cut(s) 774
XapI RAATTY 3 cut(s) 293, 397, 577
XmnI GAANNNNTTC 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.