Rh1DG294300

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0019344
Physical Location & Seq
Forward (+)
52024940 .. 52042560
17621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG294300.1

Sequence Viewer

Length: 855 bp
ATGAAGATGGACAGGGGAACTTATGTCATCGTAAAGGAAGATATGAAGTTCCTCCCAAGTGAGGATCAGCTCATCAAATTTTTGAAGATGAAGATTGATGGTAGATACTCTGGGTTCATATCCGAAATCAACTTATACAAGCACGAGCCATGGGATTTGCCCGCATTATCAGTGGTAAAGTTCAGTGAAGAGTGGTTCTTCTTCACACAGAATAGCAGAAAGTACAAGACAAGCGATCGAAGCAACAGACAAACAGAGAAGGGTTATTGGCATGAAACTTCCAAGGAAATACCAGTGGTCAATACTAATGACAAGTCTAAAGTCATTGGCAGAAAACGATTTCTAGTATTCTACAAAGGTCGTGCTTCTCGTAAAGAAAAGACTAACTGGGTTATGCAGGAGTATTTTGTTCCTGAGGCTGAGCTCGAGACGAACTTAGTGCTTTGTCGCATAAAGCAGAAATCAGTGGGGAGTTCTCACGATCGTGAAGATAGTAACAACGCATTATCATTGATAGAGTCGAATAAAGAGTGGTTTTTCTTCAATCGGGGGAGAGGCAGAACAACTAAACAAGGTTACTGGAAGAAAACTGGATCTGGCCGTAAAATAAAGGCTTCAGATAAATCGATCATTAGTGAACTGCGGCACATGACATTCCATTGCTCTCCCAAAGGGCAGGTGACCGCATGGGTTATACACGAGTATTCTCTTCTTCCCGACCAGGGGGACATAGTTTTGTGTCGGTTAAAGTTAAAGTCAGTTGACGATACTGCAACTACTTCTGATGATTTGGATATGGAGGACGTTCCAGAGGAAGATCATGGCCGCGTGGAACGCAATGCTTTCGGGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005886 GO:0006355 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009314 GO:0009416 GO:0009626 GO:0009628 GO:0009648 GO:0009651 GO:0009719 GO:0009725 GO:0009739 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009910 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010648 GO:0010769 GO:0012501 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0022603 GO:0022604 GO:0023051 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033619 GO:0033993 GO:0034050 GO:0034976 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045595 GO:0045893 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048573 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0060284 GO:0061458 GO:0065007 GO:0071214 GO:0071470 GO:0071472 GO:0071704 GO:0071944 GO:0080090 GO:0090351 GO:0097159 GO:0104004 GO:0140110 GO:1901363 GO:1901564 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000024 GO:2000026 GO:2000039 GO:2000112 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

284

Amino Acids

33.22

Weight (kDa)

9.1

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 14 - 135 2.4e-31 No apical meristem (NAM) protein
NAM PF02365 174 - 237 1e-10 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019344)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0361721 RchiOBHm_Chr1g0361731
rosa_laevigata RLG00000027707
rosa_roxburghii Rroxscaffold_4G00294740
rosa_rugosa Rorug01G0291100
rosa_samantha Rh1AG301500 Rh1BG264300 Rh1DG294300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 667
Acc36I ACCTGC 1 cut(s) 667
AccII CGCG 1 cut(s) 828
AciI CCGC 4 cut(s) 162, 643, 684, 826
AclWI GGATC 2 cut(s) 72, 601
AcoI YGGCCR 2 cut(s) 598, 823
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 1 cut(s) 600
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 3 cut(s) 61, 722, 723
AgsI TTSAA 2 cut(s) 85, 544
AjnI CCWGG 1 cut(s) 720
AleI CACNNNNGTG 1 cut(s) 483
AluBI AGCT 2 cut(s) 70, 424
AluI AGCT 2 cut(s) 70, 424
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 1 cut(s) 422
AlwI GGATC 2 cut(s) 72, 601
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 2 cut(s) 598, 823
ApoI RAATTY 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 691
AvaI CYCGRG 1 cut(s) 425
AxyI CCTNAGG 1 cut(s) 414
BanII GRGCYC 1 cut(s) 426
BauI CACGAG 2 cut(s) 143, 698
Bbv12I GWGCWC 1 cut(s) 426
BccI CCATC 1 cut(s) 92
BceAI ACGGC 1 cut(s) 585
BcgI CGANNNNNNTGC 3 cut(s) 421, 455, 826
BciT130I CCWGG 1 cut(s) 722
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 1 cut(s) 344
BfuAI ACCTGC 1 cut(s) 667
BisI GCNGC 2 cut(s) 644, 826
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 2 cut(s) 645, 827
Bme1390I CCNGG 1 cut(s) 722
BmeT110I CYCGRG 1 cut(s) 425
BmrFI CCNGG 1 cut(s) 722
BmrI ACTGGG 1 cut(s) 397
BmuI ACTGGG 1 cut(s) 397
Bpu1102I GCTNAGC 1 cut(s) 420
Bsa29I ATCGAT 1 cut(s) 626
BsaJI CCNNGG 3 cut(s) 149, 282, 721
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 722, 723
Bse1I ACTGG 4 cut(s) 293, 392, 584, 595
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 2 cut(s) 658, 844
BseBI CCWGG 1 cut(s) 722
BseCI ATCGAT 1 cut(s) 626
BseDI CCNNGG 3 cut(s) 149, 282, 721
BseLI CCNNNNNNNGG 3 cut(s) 61, 722, 723
BseMI GCAATG 2 cut(s) 658, 844
BseMII CTCAG 2 cut(s) 405, 411
BseNI ACTGG 4 cut(s) 293, 392, 584, 595
Bsh1236I CGCG 1 cut(s) 828
Bsh1285I CGRYCG 2 cut(s) 238, 484
BshFI GGCC 2 cut(s) 600, 825
BshVI ATCGAT 1 cut(s) 626
BsiEI CGRYCG 2 cut(s) 238, 484
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 425
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 3 cut(s) 61, 722, 723
BsmAI GTCTC 1 cut(s) 422
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 1 cut(s) 740
BsnI GGCC 2 cut(s) 600, 825
BsoBI CYCGRG 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 6 cut(s) 64, 235, 481, 593, 627, 817
Bsp1720I GCTNAGC 1 cut(s) 420
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 4 cut(s) 162, 643, 684, 826
BspANI GGCC 2 cut(s) 600, 825
BspCNI CTCAG 2 cut(s) 406, 412
BspDI ATCGAT 1 cut(s) 626
BspFNI CGCG 1 cut(s) 828
BspMI ACCTGC 1 cut(s) 667
BspPI GGATC 2 cut(s) 72, 601
BsrDI GCAATG 2 cut(s) 658, 844
BsrI ACTGG 4 cut(s) 293, 392, 584, 595
BssECI CCNNGG 3 cut(s) 149, 282, 721
BssMI GATC 6 cut(s) 64, 235, 481, 593, 627, 817
BssSI CACGAG 2 cut(s) 143, 698
BssT1I CCWWGG 2 cut(s) 149, 282
Bst2BI CACGAG 2 cut(s) 143, 698
Bst2UI CCWGG 1 cut(s) 722
Bst6I CTCTTC 2 cut(s) 183, 714
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 3 cut(s) 414, 420, 436
BstDSI CCRYGG 1 cut(s) 149
BstEII GGTNACC 1 cut(s) 679
BstFNI CGCG 1 cut(s) 828
BstKTI GATC 6 cut(s) 67, 238, 484, 596, 630, 820
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 6 cut(s) 64, 235, 481, 593, 627, 817
BstMCI CGRYCG 2 cut(s) 238, 484
BstMWI GCNNNNNNNGC 2 cut(s) 240, 834
BstNI CCWGG 1 cut(s) 722
BstPI GGTNACC 1 cut(s) 679
BstSCI CCNGG 1 cut(s) 720
BstUI CGCG 1 cut(s) 828
BstX2I RGATCY 1 cut(s) 593
BstYI RGATCY 1 cut(s) 593
Bsu15I ATCGAT 1 cut(s) 626
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 2 cut(s) 600, 825
BsuTUI ATCGAT 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 149
BtsIMutI CAGTG 4 cut(s) 177, 190, 300, 471
BveI ACCTGC 1 cut(s) 667
Cac8I GCNNGC 1 cut(s) 162
ClaI ATCGAT 1 cut(s) 626
Csp6I GTAC 1 cut(s) 223
CviAII CATG 5 cut(s) 150, 272, 649, 687, 821
CviJI RGCY 7 cut(s) 70, 148, 419, 424, 600, 614, 825
CviKI_1 RGCY 7 cut(s) 70, 148, 419, 424, 600, 614, 825
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 3 cut(s) 414, 420, 436
DpnI GATC 6 cut(s) 66, 237, 483, 595, 629, 819
DpnII GATC 6 cut(s) 64, 235, 481, 593, 627, 817
EaeI YGGCCR 2 cut(s) 598, 823
Eam1104I CTCTTC 2 cut(s) 183, 714
EarI CTCTTC 2 cut(s) 183, 714
Ecl136II GAGCTC 1 cut(s) 424
Eco130I CCWWGG 2 cut(s) 149, 282
Eco24I GRGCYC 1 cut(s) 426
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 600
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 425
Eco91I GGTNACC 1 cut(s) 679
EcoICRI GAGCTC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 679
EcoRII CCWGG 1 cut(s) 720
EcoT14I CCWWGG 2 cut(s) 149, 282
EcoT38I GRGCYC 1 cut(s) 426
ErhI CCWWGG 2 cut(s) 149, 282
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 5 cut(s) 153, 275, 652, 690, 824
FaqI GGGAC 1 cut(s) 740
FatI CATG 5 cut(s) 149, 271, 648, 686, 820
FauI CCCGC 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 644, 826
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 2 cut(s) 644, 826
FspBI CTAG 1 cut(s) 344
GluI GCNGC 2 cut(s) 644, 826
HaeIII GGCC 2 cut(s) 600, 825
Hin1II CATG 5 cut(s) 153, 275, 652, 690, 824
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HinfI GANTC 1 cut(s) 518
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 2 cut(s) 638, 763
Hpy188I TCNGA 3 cut(s) 124, 619, 784
Hpy188III TCNNGA 6 cut(s) 413, 427, 479, 485, 716, 809
Hpy8I GTNNAC 2 cut(s) 638, 763
HpyAV CCTTC 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 804
HpyCH4V TGCA 2 cut(s) 397, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 240, 834
HpyF3I CTNAG 3 cut(s) 414, 420, 436
HpySE526I ACGT 1 cut(s) 804
Hsp92II CATG 5 cut(s) 153, 275, 652, 690, 824
Kzo9I GATC 6 cut(s) 64, 235, 481, 593, 627, 817
MaeI CTAG 1 cut(s) 344
MaeII ACGT 1 cut(s) 804
MaeIII GTNAC 3 cut(s) 494, 575, 679
MalI GATC 6 cut(s) 66, 237, 483, 595, 629, 819
MboI GATC 6 cut(s) 64, 235, 481, 593, 627, 817
MflI RGATCY 1 cut(s) 593
MhlI GDGCHC 1 cut(s) 426
MluCI AATT 1 cut(s) 77
MlyI GAGTC 1 cut(s) 527
MnlI CCTC 6 cut(s) 55, 62, 409, 548, 793, 805
MseI TTAA 2 cut(s) 746, 752
MslI CAYNNNNRTG 1 cut(s) 483
MspR9I CCNGG 1 cut(s) 722
MvaI CCWGG 1 cut(s) 722
MvnI CGCG 1 cut(s) 828
MwoI GCNNNNNNNGC 2 cut(s) 240, 834
NcoI CCATGG 1 cut(s) 149
NdeII GATC 6 cut(s) 64, 235, 481, 593, 627, 817
NlaIII CATG 5 cut(s) 153, 275, 652, 690, 824
NmuCI GTSAC 1 cut(s) 679
OliI CACNNNNGTG 1 cut(s) 483
PaeR7I CTCGAG 1 cut(s) 425
PaqCI CACCTGC 1 cut(s) 667
PcsI WCGNNNNNNNCGW 1 cut(s) 367
PkrI GCNGC 2 cut(s) 645, 827
Ple19I CGATCG 2 cut(s) 238, 484
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Psp124BI GAGCTC 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 720
PspEI GGTNACC 1 cut(s) 679
PspGI CCWGG 1 cut(s) 720
PsuI RGATCY 1 cut(s) 593
PvuI CGATCG 2 cut(s) 238, 484
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 483
SacI GAGCTC 1 cut(s) 426
SaqAI TTAA 2 cut(s) 746, 752
SatI GCNGC 2 cut(s) 644, 826
Sau3AI GATC 6 cut(s) 64, 235, 481, 593, 627, 817
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 1 cut(s) 722
SduI GDGCHC 1 cut(s) 426
SetI ASST 6 cut(s) 72, 361, 426, 577, 681, 807
Sfr274I CTCGAG 1 cut(s) 425
SlaI CTCGAG 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 1 cut(s) 77
SsiI CCGC 4 cut(s) 162, 643, 684, 826
SspMI CTAG 1 cut(s) 344
SstI GAGCTC 1 cut(s) 426
StyD4I CCNGG 1 cut(s) 720
StyI CCWWGG 2 cut(s) 149, 282
TaiI ACGT 1 cut(s) 807
TaqI TCGA 4 cut(s) 238, 426, 521, 626
TasI AATT 1 cut(s) 77
TatI WGTACW 1 cut(s) 222
TauI GCSGC 2 cut(s) 646, 828
Tru1I TTAA 2 cut(s) 746, 752
Tru9I TTAA 2 cut(s) 746, 752
TscAI CASTG 4 cut(s) 177, 190, 300, 471
TseFI GTSAC 1 cut(s) 679
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 5 cut(s) 17, 59, 104, 106, 288
TspRI CASTG 4 cut(s) 177, 190, 300, 471
XapI RAATTY 1 cut(s) 77
XhoI CTCGAG 1 cut(s) 425
XspI CTAG 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.