Rh1DG446000

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0019000
Physical Location & Seq
Reverse (-)
65437333 .. 65449169
11837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG446000.1

Sequence Viewer

Length: 885 bp
ATGCCAAATTTCGAAAACATGTACGAAACACAGCTTATCAAAGAGATTGTGCAAGAACTGTGGAGCAAAGTGCATCCTAGTCTCACAGCATTTGGTTCCTCAGAGAAGTTATTTGGAATGGATTCTAAGCTGGAGGAGATGGATAGACTAGGACTAGGACTAGGGACTATGCCAGTCTCAGAATATCGGGACCTGCATGTACGTTCCTCTATCAACTCCAACACAGATATTAGCTTCTTCCACATGCTCAAGGATGAAGCTGTTATTCTGATCAGAAGTAATGGCTTGTCAGATGTGAAACTCCTGAGTTTGGAAAGATCATTTTCATGTCCAGTTCCCGGGGAAACTATGTTTAAGTTCCTGTACATGTTTAAGTTTACTCATATTGAGATCTGTAGACCTCACGAGCTCACTGGCGACGTTGAAGACAAAGGCGTGCCTGGTCGTCGTCATGGTGGTGATCATCTCTCTGCAAATCTCGTTGAACAATTTGAGAACTGGCGAATCTCATTGAGCTTGGCATCTACAGCGGTTTCGCGGCGTTGGCGCCTAATATCCAAAGAAACCTTTACCTTCTCTCTGCTAAACAGGATTTGCGGTGAGTCAGGTAGAGCTAATTCCTTCAAGGCCATTCAGGAAAATTTTGGTTTCAGATCTATAAGAGATAGTCATGAGGATCTAATGGATTCCTTGTACCTACAAGTCAAAATTGTGAGCCAGACTCTTATTTTTGTCACTCAATCACGAAGTTGGTCCTTTTTAGAATGCCTTGGACTACTATTACTCAATGCTGTTGCCATCGCCCAGCTGGTTTGCTTTGTAGTTTTGAGTTCTATCTCTCAGTGCACTTTCTTCGGACATCTTTGCCAAACTACAATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

294

Amino Acids

33.43

Weight (kDa)

6.15

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019000)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0395241
rosa_laevigata RLG00000009639
rosa_samantha Rh1DG445900 Rh1DG446000 Rh4AG057500 Rh4AG057600 Rh4BG056400 Rh6BG208600
rosa_wichuraiana Rw4G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 546
AccI GTMKAC 1 cut(s) 397
AccII CGCG 1 cut(s) 538
AciI CCGC 3 cut(s) 530, 538, 597
AclWI GGATC 1 cut(s) 684
AcsI RAATTY 2 cut(s) 7, 640
AcyI GRCGYC 1 cut(s) 547
AfaI GTAC 4 cut(s) 23, 201, 365, 695
AfiI CCNNNNNNNGG 2 cut(s) 310, 338
AflIII ACRYGT 2 cut(s) 18, 366
AgsI TTSAA 3 cut(s) 425, 485, 625
AjnI CCWGG 1 cut(s) 439
AluBI AGCT 8 cut(s) 34, 130, 234, 260, 409, 516, 614, 808
AluI AGCT 8 cut(s) 34, 130, 234, 260, 409, 516, 614, 808
Alw21I GWGCWC 2 cut(s) 411, 848
Alw26I GTCTC 2 cut(s) 86, 181
Alw44I GTGCAC 1 cut(s) 844
AlwI GGATC 1 cut(s) 684
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 1 cut(s) 627
ApaLI GTGCAC 1 cut(s) 844
ApoI RAATTY 2 cut(s) 7, 640
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 1 cut(s) 121
AspLEI GCGC 1 cut(s) 549
AspS9I GGNCC 2 cut(s) 190, 753
AsuC2I CCSGG 2 cut(s) 339, 340
AsuHPI GGTGA 2 cut(s) 470, 611
AsuII TTCGAA 1 cut(s) 12
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 2 cut(s) 190, 753
BaeGI GKGCMC 1 cut(s) 848
BanI GGYRCC 1 cut(s) 546
BanII GRGCYC 1 cut(s) 411
BauI CACGAG 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 411, 848
BccI CCATC 2 cut(s) 133, 806
BcgI CGANNNNNNTGC 2 cut(s) 835, 869
BciT130I CCWGG 1 cut(s) 441
BclI TGATCA 2 cut(s) 270, 460
BcnI CCSGG 2 cut(s) 339, 340
BcoDI GTCTC 2 cut(s) 86, 181
BfaI CTAG 4 cut(s) 78, 149, 155, 161
BfmI CTRYAG 2 cut(s) 394, 525
BfoI RGCGCY 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 201
BglII AGATCT 2 cut(s) 390, 653
BisI GCNGC 1 cut(s) 539
BlsI GCNGC 1 cut(s) 540
Bme1390I CCNGG 3 cut(s) 339, 340, 441
Bme18I GGWCC 2 cut(s) 190, 753
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 190, 753
BmiI GGNNCC 3 cut(s) 97, 191, 548
BmrFI CCNGG 3 cut(s) 339, 340, 441
BmsI GCATC 2 cut(s) 82, 530
BpiI GAAGAC 1 cut(s) 432
BplI GAGNNNNNCTC 2 cut(s) 706, 738
BpmI CTGGAG 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 2 cut(s) 339, 340
BsaHI GRCGYC 1 cut(s) 547
BsaJI CCNNGG 3 cut(s) 338, 339, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 338
Bse1I ACTGG 4 cut(s) 173, 332, 418, 503
BseBI CCWGG 1 cut(s) 441
BseDI CCNNGG 3 cut(s) 338, 339, 769
BseGI GGATG 2 cut(s) 73, 259
BseLI CCNNNNNNNGG 2 cut(s) 310, 338
BseMII CTCAG 4 cut(s) 114, 192, 296, 854
BseNI ACTGG 4 cut(s) 173, 332, 418, 503
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 848
BseYI CCCAGC 1 cut(s) 804
Bsh1236I CGCG 1 cut(s) 538
BshFI GGCC 1 cut(s) 629
BshNI GGYRCC 1 cut(s) 546
BsiHKAI GWGCWC 2 cut(s) 411, 848
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 1 cut(s) 339
BslFI GGGAC 2 cut(s) 178, 203
BslI CCNNNNNNNGG 2 cut(s) 310, 338
BsmAI GTCTC 2 cut(s) 86, 181
BsmFI GGGAC 2 cut(s) 178, 203
BsmI GAATGC 1 cut(s) 770
BsnI GGCC 1 cut(s) 629
BsoBI CYCGRG 1 cut(s) 338
Bsp119I TTCGAA 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 411, 848
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 6 cut(s) 270, 317, 390, 460, 653, 676
BspACI CCGC 3 cut(s) 530, 538, 597
BspANI GGCC 1 cut(s) 629
BspCNI CTCAG 4 cut(s) 113, 191, 297, 853
BspFNI CGCG 1 cut(s) 538
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 3 cut(s) 97, 191, 548
BspMI ACCTGC 1 cut(s) 201
BspPI GGATC 1 cut(s) 684
BspT104I TTCGAA 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 546
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 4 cut(s) 173, 332, 418, 503
BssECI CCNNGG 3 cut(s) 338, 339, 769
BssMI GATC 6 cut(s) 270, 317, 390, 460, 653, 676
BssNI GRCGYC 1 cut(s) 547
BssSI CACGAG 1 cut(s) 404
BssT1I CCWWGG 1 cut(s) 769
Bst2BI CACGAG 1 cut(s) 404
Bst2UI CCWGG 1 cut(s) 441
Bst4CI ACNGT 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 547
BstAUI TGTACA 1 cut(s) 363
BstBI TTCGAA 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 437
BstDEI CTNAG 5 cut(s) 100, 126, 178, 305, 840
BstF5I GGATG 2 cut(s) 73, 259
BstFNI CGCG 1 cut(s) 538
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 1 cut(s) 549
BstKTI GATC 6 cut(s) 273, 320, 393, 463, 656, 679
BstMAI GTCTC 2 cut(s) 86, 181
BstMBI GATC 6 cut(s) 270, 317, 390, 460, 653, 676
BstMWI GCNNNNNNNGC 2 cut(s) 527, 544
BstNI CCWGG 1 cut(s) 441
BstNSI RCATGY 4 cut(s) 22, 200, 247, 370
BstSCI CCNGG 3 cut(s) 337, 338, 439
BstSFI CTRYAG 2 cut(s) 394, 525
BstSLI GKGCMC 1 cut(s) 848
BstUI CGCG 1 cut(s) 538
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 3 cut(s) 390, 653, 676
BstYI RGATCY 3 cut(s) 390, 653, 676
BsuRI GGCC 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 784
BtsCI GGATG 2 cut(s) 73, 259
BtsIMutI CAGTG 2 cut(s) 411, 848
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 437
CciI TCATGA 1 cut(s) 670
CfoI GCGC 1 cut(s) 549
Cfr13I GGNCC 2 cut(s) 190, 753
Cfr9I CCCGGG 1 cut(s) 338
Csp6I GTAC 4 cut(s) 22, 200, 364, 694
CspCI CAANNNNNGTGG 2 cut(s) 41, 76
CviAII CATG 7 cut(s) 19, 197, 244, 327, 367, 452, 671
CviQI GTAC 4 cut(s) 22, 200, 364, 694
DdeI CTNAG 5 cut(s) 100, 126, 178, 305, 840
DinI GGCGCC 1 cut(s) 548
DpnI GATC 6 cut(s) 272, 319, 392, 462, 655, 678
DpnII GATC 6 cut(s) 270, 317, 390, 460, 653, 676
Ecl136II GAGCTC 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 769
Eco24I GRGCYC 1 cut(s) 411
Eco47I GGWCC 2 cut(s) 190, 753
Eco53kI GAGCTC 1 cut(s) 409
Eco88I CYCGRG 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 409
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 769
EcoT38I GRGCYC 1 cut(s) 411
EgeI GGCGCC 1 cut(s) 548
EheI GGCGCC 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 769
FaeI CATG 7 cut(s) 22, 200, 247, 330, 370, 455, 674
FaqI GGGAC 2 cut(s) 178, 203
FatI CATG 7 cut(s) 18, 196, 243, 326, 366, 451, 670
FbaI TGATCA 2 cut(s) 270, 460
FblI GTMKAC 1 cut(s) 397
Fnu4HI GCNGC 1 cut(s) 539
FokI GGATG 2 cut(s) 60, 266
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 1 cut(s) 539
FspBI CTAG 4 cut(s) 78, 149, 155, 161
GlaI GCGC 1 cut(s) 548
GluI GCNGC 1 cut(s) 539
GsaI CCCAGC 1 cut(s) 808
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 1 cut(s) 629
HapII CCGG 1 cut(s) 339
HhaI GCGC 1 cut(s) 549
Hin1I GRCGYC 1 cut(s) 547
Hin1II CATG 7 cut(s) 22, 200, 247, 330, 370, 455, 674
Hin6I GCGC 1 cut(s) 547
HinP1I GCGC 1 cut(s) 547
HinfI GANTC 5 cut(s) 122, 504, 602, 686, 721
HpaII CCGG 1 cut(s) 339
HphI GGTGA 2 cut(s) 470, 611
Hpy166II GTNNAC 3 cut(s) 378, 398, 846
Hpy188I TCNGA 7 cut(s) 103, 181, 270, 275, 292, 653, 857
Hpy188III TCNNGA 6 cut(s) 188, 304, 404, 635, 671, 744
Hpy8I GTNNAC 3 cut(s) 378, 398, 846
Hpy99I CGWCG 2 cut(s) 422, 450
HpyAV CCTTC 2 cut(s) 583, 631
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4IV ACGT 2 cut(s) 202, 420
HpyCH4V TGCA 5 cut(s) 52, 73, 196, 473, 846
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 544
HpyF3I CTNAG 5 cut(s) 100, 126, 178, 305, 840
HpySE526I ACGT 2 cut(s) 202, 420
Hsp92I GRCGYC 1 cut(s) 547
Hsp92II CATG 7 cut(s) 22, 200, 247, 330, 370, 455, 674
HspAI GCGC 1 cut(s) 547
KasI GGCGCC 1 cut(s) 546
Ksp22I TGATCA 2 cut(s) 270, 460
Kzo9I GATC 6 cut(s) 270, 317, 390, 460, 653, 676
LmnI GCTCC 1 cut(s) 63
LweI GCATC 2 cut(s) 82, 530
MaeI CTAG 4 cut(s) 78, 149, 155, 161
MaeII ACGT 2 cut(s) 202, 420
MaeIII GTNAC 1 cut(s) 733
MalI GATC 6 cut(s) 272, 319, 392, 462, 655, 678
MboI GATC 6 cut(s) 270, 317, 390, 460, 653, 676
MboII GAAGA 3 cut(s) 229, 437, 844
MflI RGATCY 3 cut(s) 390, 653, 676
MhlI GDGCHC 2 cut(s) 411, 848
MluCI AATT 7 cut(s) 7, 488, 616, 640, 708, 876, 880
Mly113I GGCGCC 1 cut(s) 547
MlyI GAGTC 2 cut(s) 611, 715
MmeI TCCRAC 1 cut(s) 243
MnlI CCTC 5 cut(s) 109, 127, 217, 411, 667
MroXI GAANNNNTTC 1 cut(s) 121
MseI TTAA 3 cut(s) 354, 372, 879
MslI CAYNNNNRTG 2 cut(s) 325, 456
MspA1I CMGCKG 2 cut(s) 530, 808
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 3 cut(s) 339, 340, 441
Mva1269I GAATGC 1 cut(s) 770
MvaI CCWGG 1 cut(s) 441
MvnI CGCG 1 cut(s) 538
MwoI GCNNNNNNNGC 2 cut(s) 527, 544
NarI GGCGCC 1 cut(s) 547
NciI CCSGG 2 cut(s) 339, 340
NdeII GATC 6 cut(s) 270, 317, 390, 460, 653, 676
NlaIII CATG 7 cut(s) 22, 200, 247, 330, 370, 455, 674
NlaIV GGNNCC 3 cut(s) 97, 191, 548
NmuCI GTSAC 1 cut(s) 733
NspI RCATGY 4 cut(s) 22, 200, 247, 370
NspV TTCGAA 1 cut(s) 12
PagI TCATGA 1 cut(s) 670
PciI ACATGT 2 cut(s) 18, 366
PctI GAATGC 1 cut(s) 770
PdmI GAANNNNTTC 1 cut(s) 121
PfeI GAWTC 3 cut(s) 122, 504, 686
PkrI GCNGC 1 cut(s) 540
PleI GAGTC 2 cut(s) 610, 715
PluTI GGCGCC 1 cut(s) 550
PpsI GAGTC 2 cut(s) 610, 715
PpuMI RGGWCCY 1 cut(s) 190
PscI ACATGT 2 cut(s) 18, 366
PshBI ATTAAT 1 cut(s) 879
Psp124BI GAGCTC 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 439
PspFI CCCAGC 1 cut(s) 804
PspGI CCWGG 1 cut(s) 439
PspN4I GGNNCC 3 cut(s) 97, 191, 548
PspPI GGNCC 2 cut(s) 190, 753
PspPPI RGGWCCY 1 cut(s) 190
PsuI RGATCY 3 cut(s) 390, 653, 676
PvuII CAGCTG 1 cut(s) 808
RsaI GTAC 4 cut(s) 23, 201, 365, 695
RsaNI GTAC 4 cut(s) 22, 200, 364, 694
RseI CAYNNNNRTG 2 cut(s) 325, 456
SacI GAGCTC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 354, 372, 879
SatI GCNGC 1 cut(s) 539
Sau3AI GATC 6 cut(s) 270, 317, 390, 460, 653, 676
Sau96I GGNCC 2 cut(s) 190, 753
SchI GAGTC 2 cut(s) 611, 715
ScrFI CCNGG 3 cut(s) 339, 340, 441
SduI GDGCHC 2 cut(s) 411, 848
SfaNI GCATC 2 cut(s) 82, 530
SfcI CTRYAG 2 cut(s) 394, 525
SfoI GGCGCC 1 cut(s) 548
SfuI TTCGAA 1 cut(s) 12
SinI GGWCC 2 cut(s) 190, 753
SmaI CCCGGG 1 cut(s) 340
SmiMI CAYNNNNRTG 2 cut(s) 325, 456
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 7 cut(s) 7, 488, 616, 640, 708, 876, 880
SsiI CCGC 3 cut(s) 530, 538, 597
SspDI GGCGCC 1 cut(s) 546
SspMI CTAG 4 cut(s) 78, 149, 155, 161
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 3 cut(s) 337, 338, 439
StyI CCWWGG 1 cut(s) 769
TaaI ACNGT 1 cut(s) 60
TaiI ACGT 2 cut(s) 205, 423
TaqI TCGA 1 cut(s) 12
TasI AATT 7 cut(s) 7, 488, 616, 640, 708, 876, 880
TatI WGTACW 1 cut(s) 363
TauI GCSGC 1 cut(s) 541
TfiI GAWTC 3 cut(s) 122, 504, 686
Tru1I TTAA 3 cut(s) 354, 372, 879
Tru9I TTAA 3 cut(s) 354, 372, 879
TscAI CASTG 2 cut(s) 418, 848
TseFI GTSAC 1 cut(s) 733
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 2 cut(s) 270, 315
TspMI CCCGGG 1 cut(s) 338
TspRI CASTG 2 cut(s) 418, 848
VneI GTGCAC 1 cut(s) 844
VpaK11BI GGWCC 2 cut(s) 190, 753
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 2 cut(s) 7, 640
XceI RCATGY 4 cut(s) 22, 200, 247, 370
XcmI CCANNNNNNNNNTGG 1 cut(s) 805
XmaI CCCGGG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 397
XmnI GAANNNNTTC 1 cut(s) 121
XspI CTAG 4 cut(s) 78, 149, 155, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.