Rh2AG100500

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019868
Physical Location & Seq
Reverse (-)
8567530 .. 8574090
6561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG100500.1

Sequence Viewer

Length: 648 bp
ATGGCTGGTATTGTGACACATTCAAGCCAAGTAACCCCAACACTCCTAAGATGTGGACTGAAAATTGGACAGGCTGGTAAATTTACACGTGAATATTTATTGCCAAATTTAAGTATGTGGCGTTTGATTGTTAATTTCCTAAATAATTGGTATGCATGGGGTGGTTTAGACCCACTCAGGACTGTTGAGGACGTTGCCTTTGCAGTAGCAAGGTTTTTCCAATTTGAAGGCACATTCCAAAACTATTACATGGTAAACACCCAAACTACAGTGAAAGTAAAAAGTGTTAACAAGGCTGTGGATGAACCTGAATCCCTTCATTGGTCATGGAGGCCCGAGAAGACGGATGACACCCAGCTTCTTGGAAAAGGTGAAGTTGTGGAAAATCATCTTAAGGAACAAAAAATGCTGCAAATGATGCTAGCAACTTTCAAAGCTCCCCTAGGAACTGAACCAGTCGCAGTGGACTTTCACGGGTTGGGTAAGGGCGATGCTTGGGTGAACGGGCATAGCGTTGGGCGATATTGGCCAAGCTACTTGGCTCCAAAGGATGGTTGTAGTGTTGAAGCTTGTGACTACCGTGGTGCATACGACAATAACAAAGATGGTAACAACATTTTTCTCAACCTAGATAATTTATTCAATTGA

Protein Analysis

215

Amino Acids

24.32

Weight (kDa)

6.2

Isoelectric Point (pI)

24.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BetaGal_gal-bd PF21467 142 - 178 9.5e-12 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019868)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0095601 RchiOBHm_Chr2g0095621
rosa_multiflora Rmu_co8127886.1_g000001 Rmu_sc0003026.1_g000002
rosa_roxburghii Rroxscaffold_2G00146160
rosa_samantha Rh2AG100500 Rh2DG101600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 551
AcoI YGGCCR 1 cut(s) 527
AcsI RAATTY 2 cut(s) 80, 106
AcvI CACGTG 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 321, 551
AflII CTTAAG 1 cut(s) 392
AflIII ACRYGT 1 cut(s) 86
AgsI TTSAA 5 cut(s) 24, 227, 433, 566, 643
AluBI AGCT 4 cut(s) 358, 437, 534, 569
AluI AGCT 4 cut(s) 358, 437, 534, 569
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 2 cut(s) 332, 527
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 2 cut(s) 80, 106
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
Asp700I GAANNNNTTC 1 cut(s) 315
AspA2I CCTAGG 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 333
AsuHPI GGTGA 2 cut(s) 383, 511
AsuNHI GCTAGC 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 335
AvrII CCTAGG 1 cut(s) 442
BalI TGGCCA 1 cut(s) 529
BbrPI CACGTG 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 347
BbvI GCAGC 1 cut(s) 396
BccI CCATC 2 cut(s) 545, 599
BfaI CTAG 3 cut(s) 422, 443, 629
BfmI CTRYAG 1 cut(s) 267
BfrI CTTAAG 1 cut(s) 392
BisI GCNGC 1 cut(s) 410
BlnI CCTAGG 1 cut(s) 442
BlsI GCNGC 1 cut(s) 411
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 543
BmsI GCATC 2 cut(s) 408, 481
BmtI GCTAGC 1 cut(s) 425
BpiI GAAGAC 1 cut(s) 347
BsaAI YACGTR 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 442, 580
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 551
Bse1I ACTGG 1 cut(s) 455
BseDI CCNNGG 2 cut(s) 442, 580
BseGI GGATG 3 cut(s) 307, 352, 556
BseLI CCNNNNNNNGG 2 cut(s) 321, 551
BseMII CTCAG 1 cut(s) 190
BseNI ACTGG 1 cut(s) 455
BseXI GCAGC 1 cut(s) 396
BseYI CCCAGC 1 cut(s) 354
BshFI GGCC 2 cut(s) 334, 529
BsiHKCI CYCGRG 1 cut(s) 335
BslI CCNNNNNNNGG 2 cut(s) 321, 551
BsnI GGCC 2 cut(s) 334, 529
BsoBI CYCGRG 1 cut(s) 335
BspANI GGCC 2 cut(s) 334, 529
BspCNI CTCAG 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 543
BspOI GCTAGC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 392
BsrI ACTGG 1 cut(s) 455
BssECI CCNNGG 2 cut(s) 442, 580
BssT1I CCWWGG 1 cut(s) 442
Bst4CI ACNGT 3 cut(s) 184, 271, 581
BstAFI CTTAAG 1 cut(s) 392
BstAPI GCANNNNNTGC 1 cut(s) 418
BstBAI YACGTR 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 2 cut(s) 47, 176
BstDSI CCRYGG 1 cut(s) 580
BstF5I GGATG 3 cut(s) 307, 352, 556
BstMWI GCNNNNNNNGC 2 cut(s) 418, 526
BstSFI CTRYAG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 396
BstV2I GAAGAC 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 362
BsuRI GGCC 2 cut(s) 334, 529
BtgI CCRYGG 1 cut(s) 580
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 3 cut(s) 307, 352, 556
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 2 cut(s) 276, 468
Cac8I GCNNGC 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 333
CviAII CATG 3 cut(s) 156, 250, 327
DdeI CTNAG 2 cut(s) 47, 176
EaeI YGGCCR 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 442
Eco72I CACGTG 1 cut(s) 89
Eco88I CYCGRG 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 3 cut(s) 159, 253, 330
FaiI YATR 7 cut(s) 116, 153, 157, 251, 328, 510, 589
FatI CATG 3 cut(s) 155, 249, 326
Fnu4HI GCNGC 1 cut(s) 410
FokI GGATG 3 cut(s) 314, 359, 563
Fsp4HI GCNGC 1 cut(s) 410
FspBI CTAG 3 cut(s) 422, 443, 629
GluI GCNGC 1 cut(s) 410
GsaI CCCAGC 1 cut(s) 358
HaeIII GGCC 2 cut(s) 334, 529
Hin1II CATG 3 cut(s) 159, 253, 330
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 1 cut(s) 311
HpaI GTTAAC 1 cut(s) 289
HphI GGTGA 2 cut(s) 383, 511
Hpy166II GTNNAC 5 cut(s) 56, 256, 289, 466, 502
Hpy188III TCNNGA 1 cut(s) 178
Hpy8I GTNNAC 5 cut(s) 56, 256, 289, 466, 502
HpyAV CCTTC 2 cut(s) 221, 326
HpyCH4III ACNGT 3 cut(s) 184, 271, 581
HpyCH4IV ACGT 2 cut(s) 88, 192
HpyCH4V TGCA 4 cut(s) 155, 203, 412, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 418, 526
HpyF3I CTNAG 2 cut(s) 47, 176
HpySE526I ACGT 2 cut(s) 88, 192
Hsp92II CATG 3 cut(s) 159, 253, 330
KspAI GTTAAC 1 cut(s) 289
LmnI GCTCC 2 cut(s) 442, 547
LpnPI CCDG 6 cut(s) 56, 60, 163, 321, 368, 468
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 2 cut(s) 408, 481
MaeI CTAG 3 cut(s) 422, 443, 629
MaeII ACGT 2 cut(s) 88, 192
MaeIII GTNAC 4 cut(s) 13, 31, 572, 608
MboII GAAGA 1 cut(s) 352
MfeI CAATTG 1 cut(s) 643
MlsI TGGCCA 1 cut(s) 529
MluCI AATT 8 cut(s) 63, 80, 106, 133, 145, 221, 634, 643
MluNI TGGCCA 1 cut(s) 529
MnlI CCTC 2 cut(s) 181, 324
Mox20I TGGCCA 1 cut(s) 529
Mph1103I ATGCAT 1 cut(s) 157
MroXI GAANNNNTTC 1 cut(s) 315
MscI TGGCCA 1 cut(s) 529
MseI TTAA 4 cut(s) 110, 132, 288, 393
Msp20I TGGCCA 1 cut(s) 529
MspCI CTTAAG 1 cut(s) 392
MunI CAATTG 1 cut(s) 643
MwoI GCNNNNNNNGC 2 cut(s) 418, 526
NheI GCTAGC 1 cut(s) 421
NlaIII CATG 3 cut(s) 159, 253, 330
NlaIV GGNNCC 1 cut(s) 543
NmuCI GTSAC 2 cut(s) 13, 572
NsiI ATGCAT 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 311
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 1 cut(s) 411
PmaCI CACGTG 1 cut(s) 89
PmlI CACGTG 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 89
PspCI CACGTG 1 cut(s) 89
PspFI CCCAGC 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 543
PspPI GGNCC 1 cut(s) 333
SaqAI TTAA 4 cut(s) 110, 132, 288, 393
SatI GCNGC 1 cut(s) 410
Sau96I GGNCC 1 cut(s) 333
SfaNI GCATC 2 cut(s) 408, 481
SfcI CTRYAG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 8 cut(s) 63, 80, 106, 133, 145, 221, 634, 643
SspI AATATT 1 cut(s) 95
SspMI CTAG 3 cut(s) 422, 443, 629
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 3 cut(s) 184, 271, 581
TaiI ACGT 2 cut(s) 91, 195
TasI AATT 8 cut(s) 63, 80, 106, 133, 145, 221, 634, 643
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 4 cut(s) 110, 132, 288, 393
Tru9I TTAA 4 cut(s) 110, 132, 288, 393
TscAI CASTG 2 cut(s) 276, 468
TseFI GTSAC 2 cut(s) 13, 572
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 2 cut(s) 13, 572
TspDTI ATGAA 2 cut(s) 308, 318
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 2 cut(s) 276, 468
Van91I CCANNNNNTGG 1 cut(s) 551
Vha464I CTTAAG 1 cut(s) 392
XapI RAATTY 2 cut(s) 80, 106
XmaJI CCTAGG 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 3 cut(s) 422, 443, 629
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.