Rh2DG101600

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0019868
Physical Location & Seq
Reverse (-)
8209038 .. 8213626
4589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG101600.1

Sequence Viewer

Length: 516 bp
ATGCAAATAACAGTTCAGATACTACAATCACTTTCCAAGGAAACACATACACCATTCCTGCCTGGTCTGTTAGTATTCTTCCTGATTACAAAAAAGAAGCATATATACAACACTGCTAAGGTAAACACCCAAACTACAGTGAAAGTAAAAAGTGTTAACAAGGCTGTGGATGAACCTGAATCCCTTCATTGGTCATGGAGGCCTGAGAAGACGGATGACACCCAGCTTCTTGGAAAAGGTGAAGTTGTGGAAAATCATCTTATGGAACAAAAAATGCTGCAAATGATGCTAGCAACTTTCAAAGCTCCCCTAGGAATTGAACCAGTCGCGATGGACTTTCACGGGTTGGGTAAGGGCCATGCTTGGGTGAACGGGCATAGCATTGGGCGATATTGGCCAAGCTACTTGGCTCCAAAGGATGGTTGTAGTGTTGAAGCTTGTGACTACCGTGGTGCATACGACAATAACAAAGATGGTAACAACATTTTTCTCAACCTAGATAATTTATTCAATTGA

Protein Analysis

171

Amino Acids

19.35

Weight (kDa)

7.05

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BetaGal_gal-bd PF21467 98 - 134 4.7e-13 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019868)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0095601 RchiOBHm_Chr2g0095621
rosa_multiflora Rmu_co8127886.1_g000001 Rmu_sc0003026.1_g000002
rosa_roxburghii Rroxscaffold_2G00146160
rosa_samantha Rh2AG100500 Rh2DG101600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 419
AccII CGCG 1 cut(s) 329
AcoI YGGCCR 1 cut(s) 395
AfiI CCNNNNNNNGG 3 cut(s) 189, 364, 419
AgsI TTSAA 4 cut(s) 301, 320, 434, 511
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 4 cut(s) 226, 305, 402, 437
AluI AGCT 4 cut(s) 226, 305, 402, 437
AoxI GGCC 3 cut(s) 200, 355, 395
ApeKI GCWGC 1 cut(s) 277
Asp700I GAANNNNTTC 1 cut(s) 183
AspA2I CCTAGG 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 355
AsuHPI GGTGA 2 cut(s) 251, 379
AsuNHI GCTAGC 1 cut(s) 289
AvrII CCTAGG 1 cut(s) 310
BalI TGGCCA 1 cut(s) 397
BarI GAAGNNNNNNTAC 2 cut(s) 89, 121
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 264
BccI CCATC 3 cut(s) 325, 413, 467
BciT130I CCWGG 1 cut(s) 63
BfaI CTAG 3 cut(s) 290, 311, 497
BfmI CTRYAG 1 cut(s) 135
BisI GCNGC 1 cut(s) 278
BlnI CCTAGG 1 cut(s) 310
BlsI GCNGC 1 cut(s) 279
Bme1390I CCNGG 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 355
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 1 cut(s) 276
BmtI GCTAGC 1 cut(s) 293
BpiI GAAGAC 1 cut(s) 215
Bpu10I CCTNAGC 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 36, 310, 448
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 364, 419
Bse1I ACTGG 1 cut(s) 323
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 3 cut(s) 36, 310, 448
BseGI GGATG 3 cut(s) 175, 220, 424
BseLI CCNNNNNNNGG 3 cut(s) 189, 364, 419
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 323
BseXI GCAGC 1 cut(s) 264
BseYI CCCAGC 1 cut(s) 222
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 3 cut(s) 202, 357, 397
BslI CCNNNNNNNGG 3 cut(s) 189, 364, 419
BsnI GGCC 3 cut(s) 202, 357, 397
Bsp68I TCGCGA 1 cut(s) 329
BspANI GGCC 3 cut(s) 202, 357, 397
BspCNI CTCAG 1 cut(s) 196
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 1 cut(s) 411
BspOI GCTAGC 1 cut(s) 293
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 36, 310, 448
BssT1I CCWWGG 2 cut(s) 36, 310
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 3 cut(s) 13, 139, 449
BstAPI GCANNNNNTGC 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 2 cut(s) 117, 204
BstDSI CCRYGG 1 cut(s) 448
BstF5I GGATG 3 cut(s) 175, 220, 424
BstFNI CGCG 1 cut(s) 329
BstMWI GCNNNNNNNGC 2 cut(s) 286, 394
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 135
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 264
BstV2I GAAGAC 1 cut(s) 215
BstXI CCANNNNNNTGG 1 cut(s) 230
BsuRI GGCC 3 cut(s) 202, 357, 397
BtgI CCRYGG 1 cut(s) 448
BtgZI GCGATG 1 cut(s) 344
BtsCI GGATG 3 cut(s) 175, 220, 424
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 144
BtuMI TCGCGA 1 cut(s) 329
Cac8I GCNNGC 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 355
CviAII CATG 2 cut(s) 195, 359
CviJI RGCY 9 cut(s) 164, 202, 226, 305, 357, 397, 402, 410, 437
CviKI_1 RGCY 9 cut(s) 164, 202, 226, 305, 357, 397, 402, 410, 437
DdeI CTNAG 2 cut(s) 117, 204
EaeI YGGCCR 1 cut(s) 395
Eco130I CCWWGG 2 cut(s) 36, 310
Eco147I AGGCCT 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 2 cut(s) 36, 310
ErhI CCWWGG 2 cut(s) 36, 310
FaeI CATG 2 cut(s) 198, 362
FaiI YATR 9 cut(s) 48, 102, 104, 106, 196, 263, 360, 378, 457
FatI CATG 2 cut(s) 194, 358
Fnu4HI GCNGC 1 cut(s) 278
FokI GGATG 3 cut(s) 182, 227, 431
Fsp4HI GCNGC 1 cut(s) 278
FspBI CTAG 3 cut(s) 290, 311, 497
GluI GCNGC 1 cut(s) 278
GsaI CCCAGC 1 cut(s) 226
HaeIII GGCC 3 cut(s) 202, 357, 397
Hin1II CATG 2 cut(s) 198, 362
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 1 cut(s) 179
HpaI GTTAAC 1 cut(s) 157
HphI GGTGA 2 cut(s) 251, 379
Hpy166II GTNNAC 3 cut(s) 124, 157, 370
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 2 cut(s) 82, 328
Hpy8I GTNNAC 3 cut(s) 124, 157, 370
HpyAV CCTTC 1 cut(s) 194
HpyCH4III ACNGT 3 cut(s) 13, 139, 449
HpyCH4V TGCA 3 cut(s) 4, 280, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 286, 394
HpyF3I CTNAG 2 cut(s) 117, 204
Hsp92II CATG 2 cut(s) 198, 362
KspAI GTTAAC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 310, 415
LpnPI CCDG 8 cut(s) 48, 71, 75, 95, 189, 216, 236, 336
Lsp1109I GCAGC 1 cut(s) 264
LweI GCATC 1 cut(s) 276
MaeI CTAG 3 cut(s) 290, 311, 497
MaeIII GTNAC 2 cut(s) 440, 476
MboII GAAGA 2 cut(s) 70, 220
MfeI CAATTG 1 cut(s) 511
MlsI TGGCCA 1 cut(s) 397
MluCI AATT 3 cut(s) 315, 502, 511
MluNI TGGCCA 1 cut(s) 397
MnlI CCTC 1 cut(s) 192
Mox20I TGGCCA 1 cut(s) 397
MroXI GAANNNNTTC 1 cut(s) 183
MscI TGGCCA 1 cut(s) 397
MseI TTAA 1 cut(s) 156
Msp20I TGGCCA 1 cut(s) 397
MspR9I CCNGG 1 cut(s) 63
MunI CAATTG 1 cut(s) 511
MvaI CCWGG 1 cut(s) 63
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 2 cut(s) 286, 394
NheI GCTAGC 1 cut(s) 289
NlaIII CATG 2 cut(s) 198, 362
NlaIV GGNNCC 1 cut(s) 411
NmuCI GTSAC 1 cut(s) 440
NruI TCGCGA 1 cut(s) 329
PceI AGGCCT 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 1 cut(s) 179
PflMI CCANNNNNTGG 1 cut(s) 419
PkrI GCNGC 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 222
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 1 cut(s) 411
PspPI GGNCC 1 cut(s) 355
RruI TCGCGA 1 cut(s) 329
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 1 cut(s) 278
Sau96I GGNCC 1 cut(s) 355
ScrFI CCNGG 1 cut(s) 63
SetI ASST 8 cut(s) 123, 178, 228, 241, 307, 404, 439, 498
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 135
Sse9I AATT 3 cut(s) 315, 502, 511
SseBI AGGCCT 1 cut(s) 202
SspMI CTAG 3 cut(s) 290, 311, 497
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 2 cut(s) 36, 310
TaaI ACNGT 3 cut(s) 13, 139, 449
TasI AATT 3 cut(s) 315, 502, 511
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 118, 144
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 176, 186
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 2 cut(s) 118, 144
Van91I CCANNNNNTGG 1 cut(s) 419
XmaJI CCTAGG 1 cut(s) 310
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 3 cut(s) 290, 311, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.