Rh2AG318800

Calmodulin-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018983
Physical Location & Seq
Forward (+)
45446548 .. 45493100
46553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG318800.1

Sequence Viewer

Length: 774 bp
ATGCCTTCAAAGGGTAAGAAGAACTCAAAGGCCCAATCGAGGCTGTCCAATTCTAACAGCTTTGCCTCTTTTGGGTCGATTGATTTTGAACCCAATGAAGTGGGTTCTGCCATCTCTCTTGAACAAGCTTCCAGCAAATACCTATCTCTGATTGGGAAATTTGCTTTCATTGGTCAAGTCACCGATGTCGATGACACCTCCAAAGGCTATAAAATTTGGCTTTCAGAGCCTTCCATGGTTGCCAATTCCTTCACTCCTGGCTCTATTGTATCGTACATGCTGTTTGTCTTTTTACTCCTCATGCTTGAAACTGGAGTGACTGCTGATATATATTTAAACCATGACTGGGAAGAGAAACTCAGACGAATGTTTTCGACGCTGGTTCTCAAAAGGTCGGAGTTGGCTTTGCCCGATTTTTATGGTCAAGACTCAATGTTAGTATTTGCTAATGCATTTTGCATCATTCTCGGGGAAGACTTTCCCGTCCTGATTCGACCTGAGATGATTGAGATCAAGTTCCCCTCCCAGCTCCGCGACTCCCTCGACTTCTTGAAATTCATCGAGGACAACAATCAAAATGACGATGCTTTGAGCTGTGTTGACGTCAGTCGGTGCTTCGTGGCAGGAGAGAGTGAGTTCAAGAAGGTGAGGATGCTGGGGCTCATTTCGATTCAACCATTCTTCGACAAAGAGGAGATAACCGAATCAAAGATCCAATTGGGTCAGGGCGCATTGGGTGTGGATTGGACGGACTGTTGTTGGAGGTGGTGGTGA

Protein Analysis

257

Amino Acids

29.07

Weight (kDa)

4.73

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_CI111 PF26429 39 - 94 1.7e-16 CI111 double-psi beta barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018983)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0126221
rosa_laevigata RLG00000002694 RLG00000007991
rosa_roxburghii Rroxscaffold_2G00117370 Rroxscaffold_2G00148290
rosa_samantha Rh1BG184500 Rh2AG318800 Rh3BG281300 Rh6BG474200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 482
AatII GACGTC 1 cut(s) 606
AccII CGCG 1 cut(s) 534
AciI CCGC 1 cut(s) 532
AclWI GGATC 1 cut(s) 706
AcsI RAATTY 3 cut(s) 158, 213, 554
AcyI GRCGYC 1 cut(s) 603
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 4 cut(s) 11, 39, 72, 346
AgsI TTSAA 7 cut(s) 9, 89, 122, 308, 553, 640, 674
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 4 cut(s) 60, 128, 529, 594
AluI AGCT 4 cut(s) 60, 128, 529, 594
AlwI GGATC 1 cut(s) 706
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 1 cut(s) 30
ApoI RAATTY 3 cut(s) 158, 213, 554
Asp700I GAANNNNTTC 2 cut(s) 370, 477
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 1 cut(s) 31
AsuHPI GGTGA 2 cut(s) 172, 658
AvaI CYCGRG 1 cut(s) 467
BanII GRGCYC 1 cut(s) 663
BbsI GAAGAC 1 cut(s) 480
BccI CCATC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 448, 482
BciT130I CCWGG 1 cut(s) 258
Bme1390I CCNGG 1 cut(s) 258
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 258
BmrI ACTGGG 1 cut(s) 355
BmsI GCATC 3 cut(s) 468, 574, 642
BmuI ACTGGG 1 cut(s) 355
BoxI GACNNNNGTC 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 480
BpmI CTGGAG 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 509
BsaHI GRCGYC 1 cut(s) 603
BsaJI CCNNGG 1 cut(s) 234
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 39, 72, 346
Bse1I ACTGG 2 cut(s) 316, 350
Bse8I GATNNNNATC 1 cut(s) 509
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 1 cut(s) 657
BseJI GATNNNNATC 1 cut(s) 509
BseLI CCNNNNNNNGG 4 cut(s) 11, 39, 72, 346
BseMII CTCAG 2 cut(s) 373, 489
BseNI ACTGG 2 cut(s) 316, 350
BseRI GAGGAG 2 cut(s) 287, 707
BseYI CCCAGC 2 cut(s) 525, 655
Bsh1236I CGCG 1 cut(s) 534
BshFI GGCC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 467
BslI CCNNNNNNNGG 4 cut(s) 11, 39, 72, 346
BsnI GGCC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 663
Bsp143I GATC 2 cut(s) 510, 711
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 1 cut(s) 532
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 2 cut(s) 372, 490
BspFNI CGCG 1 cut(s) 534
BspPI GGATC 1 cut(s) 706
BsrI ACTGG 2 cut(s) 316, 350
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 2 cut(s) 510, 711
BssNI GRCGYC 1 cut(s) 603
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 755
Bst6I CTCTTC 1 cut(s) 345
BstACI GRCGYC 1 cut(s) 603
BstDEI CTNAG 2 cut(s) 359, 498
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 1 cut(s) 657
BstFNI CGCG 1 cut(s) 534
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 2 cut(s) 513, 714
BstMBI GATC 2 cut(s) 510, 711
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 1 cut(s) 258
BstNSI RCATGY 1 cut(s) 280
BstPAI GACNNNNGTC 1 cut(s) 606
BstSCI CCNGG 1 cut(s) 256
BstUI CGCG 1 cut(s) 534
BstV2I GAAGAC 1 cut(s) 480
BstX2I RGATCY 1 cut(s) 711
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 1 cut(s) 711
BsuRI GGCC 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 1 cut(s) 657
CfoI GCGC 1 cut(s) 731
Cfr13I GGNCC 1 cut(s) 31
CseI GACGC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 274
CviAII CATG 4 cut(s) 235, 277, 301, 341
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 2 cut(s) 359, 498
DpnI GATC 2 cut(s) 512, 713
DpnII GATC 2 cut(s) 510, 711
DraI TTTAAA 1 cut(s) 336
DrdI GACNNNNNNGTC 1 cut(s) 482
DseDI GACNNNNNNGTC 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 663
Eco88I CYCGRG 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 234
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 4 cut(s) 238, 280, 304, 344
FaiI YATR 8 cut(s) 210, 236, 278, 302, 329, 331, 342, 420
FatI CATG 4 cut(s) 234, 276, 300, 340
FokI GGATG 1 cut(s) 664
FriOI GRGCYC 1 cut(s) 663
GlaI GCGC 1 cut(s) 730
GsaI CCCAGC 2 cut(s) 529, 659
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 1 cut(s) 32
HgaI GACGC 1 cut(s) 385
HhaI GCGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 603
Hin1II CATG 4 cut(s) 238, 280, 304, 344
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 5 cut(s) 428, 490, 536, 670, 704
HphI GGTGA 2 cut(s) 172, 658
Hpy166II GTNNAC 1 cut(s) 601
Hpy188I TCNGA 4 cut(s) 150, 226, 362, 397
Hpy188III TCNNGA 5 cut(s) 119, 425, 487, 550, 640
Hpy8I GTNNAC 1 cut(s) 601
Hpy99I CGWCG 1 cut(s) 379
HpyAV CCTTC 4 cut(s) 15, 240, 259, 637
HpyCH4III ACNGT 1 cut(s) 755
HpyCH4IV ACGT 1 cut(s) 603
HpyCH4V TGCA 2 cut(s) 452, 459
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 2 cut(s) 359, 498
HpySE526I ACGT 1 cut(s) 603
Hsp92I GRCGYC 1 cut(s) 603
Hsp92II CATG 4 cut(s) 238, 280, 304, 344
HspAI GCGC 1 cut(s) 729
Kzo9I GATC 2 cut(s) 510, 711
LmnI GCTCC 1 cut(s) 534
LweI GCATC 3 cut(s) 468, 574, 642
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 2 cut(s) 178, 316
MalI GATC 2 cut(s) 512, 713
MboI GATC 2 cut(s) 510, 711
MboII GAAGA 4 cut(s) 31, 362, 485, 673
MfeI CAATTG 1 cut(s) 716
MflI RGATCY 1 cut(s) 711
MhlI GDGCHC 1 cut(s) 663
MluCI AATT 6 cut(s) 49, 158, 213, 244, 554, 716
MlyI GAGTC 2 cut(s) 422, 530
MmeI TCCRAC 2 cut(s) 375, 740
Mph1103I ATGCAT 1 cut(s) 454
MroXI GAANNNNTTC 2 cut(s) 370, 477
MseI TTAA 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 716
MvaI CCWGG 1 cut(s) 258
MvnI CGCG 1 cut(s) 534
MwoI GCNNNNNNNGC 1 cut(s) 226
NcoI CCATGG 1 cut(s) 234
NdeII GATC 2 cut(s) 510, 711
NlaIII CATG 4 cut(s) 238, 280, 304, 344
NmuCI GTSAC 2 cut(s) 178, 316
NsiI ATGCAT 1 cut(s) 454
NspI RCATGY 1 cut(s) 280
PdmI GAANNNNTTC 2 cut(s) 370, 477
PfeI GAWTC 3 cut(s) 490, 670, 704
PleI GAGTC 2 cut(s) 422, 530
PpsI GAGTC 2 cut(s) 422, 530
PshAI GACNNNNGTC 1 cut(s) 606
Psp6I CCWGG 1 cut(s) 256
PspFI CCCAGC 2 cut(s) 525, 655
PspGI CCWGG 1 cut(s) 256
PspPI GGNCC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 711
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
SaqAI TTAA 1 cut(s) 335
Sau3AI GATC 2 cut(s) 510, 711
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 2 cut(s) 422, 530
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 663
SfaNI GCATC 3 cut(s) 468, 574, 642
Sse9I AATT 6 cut(s) 49, 158, 213, 244, 554, 716
SsiI CCGC 1 cut(s) 532
StyD4I CCNGG 1 cut(s) 256
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 755
TaiI ACGT 1 cut(s) 606
TaqI TCGA 9 cut(s) 38, 77, 189, 374, 493, 543, 561, 668, 684
TasI AATT 6 cut(s) 49, 158, 213, 244, 554, 716
TfiI GAWTC 3 cut(s) 490, 670, 704
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TseFI GTSAC 2 cut(s) 178, 316
Tsp45I GTSAC 2 cut(s) 178, 316
TspDTI ATGAA 3 cut(s) 111, 157, 547
TspGWI ACGGA 1 cut(s) 764
XapI RAATTY 3 cut(s) 158, 213, 554
XceI RCATGY 1 cut(s) 280
XmnI GAANNNNTTC 2 cut(s) 370, 477
ZraI GACGTC 1 cut(s) 604
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.