Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0015938

Physical Location & Seq

Genomic Coordinates Forward (+)

57783064 .. 57788109

Gene Structure 5046 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG386800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGGCGGTTTCAGCTAATACCTCTCAAATTCCTCACAGCCAACACCTCCCAATTCCTCAGTTCCCCAAAACCCACAAGCTCGAAACCCTAAGCTTCTCCACCAAGCTCCAAAGTCCCCATATTTCTTCTAGAAAAACCCAAGAACTCACTGTCTGCAGCACCTCCTCCGTCACCACCACCACCACCAACACCCAGAACCCCAACTCCGACCTAAACGAGCTCTGCCGCCATGGGAACCTCGAGAAAGCTCTGAAGCTCTTGGACTCCATGCAGGAGGTTCAGGTTAAGGTGGAAGAAGATGCTTATGTTGCTTTGGTTAGGCTATGCGAGTGGAAGAGAACACACGAGGATGGGGCTCGTGTGTACAAGTGCATATCGAATTCGATGACCCTTTTGAGCGTTAGGCTTGGTAATGCGTTGTTGAGCATGTTTGTGAGGTTTGGCAATTTGGTTGATGCTTGGTATGTGTTTGGGAGAATGGGTGAGAGGGATGTGTTTTCTTGGAATGTTTTGGTTGGTGGGTATGCGAAAGCCGGGTTTTTCGATGAGGCTTTGAACTTGTATCATAGAATGTTGTGGGTTGGGATTGTGCCTGATGTGTATACGTTTCCTTGTGTTCTCAGAACTTGTGGGGGAGTGCCGGACTTGGCGAGAGGGAGGGAGATTCATGTGCATGTGATTCGATTTGGGTTTGAGTCGGATGTCGATATTGTGAATGCTTTGATTACAATGTATGTCAAATGTGGGGTGGTTGGTAGTGCACGGGTGCTGTTCGATAGAATGCCTAGGAGAGACAGGATTTCGTGGAATGCAATGATTTCGGGGTATTTTGAGAATGGGGAGTATTTGGAAGGACTGAGATTGTTTCTCATGATGCGGGAGTTTTCAGTTGATCCGGATTTGATGACCGTGACTAGCTTGTTATCTGCTTGCGAGGTTCTTGGTGATGACAAGTTAGGGAGGGAAATACATGGTTATGTTTTGAAAACGGAGTTGGTGGACGATGTCTCGGTGTGTAGTTCTTTGATTCAGATGTATTCAGTTGTTGGGGATTTGGGGGAAGCGGAGAAAGTATTTTCCAGGATGGAAGATAAAGATGTGGTGTTATGGACATCGATGATTTCGGGTTATGTGAATAACGCACTGCCTGAGAAGGCTGTGGAAACCTACAAAGTGATGGAGCAGGAGGGTATTTTGCCAGATGAAATAACTATAGCCAGTGTTATATCTGCTTGTGCTTGTTTAGGGAACTTAGATATGGGTATCAAGCTTCATGAGCTTGCTGACAGGACTGGGCTCATATCGTATGTGATAGTTGCTAATACTCTCATTGATATGTATTCTAAGTGTAAATGCATTGACAAAGCATTGGAAGTGTTCCACCAGATCCCAGGAAAGAATGTCATATCTTGGACTTCAATCATACTCGGCCTTCGGATAAACAATCGGTGCTTTGAGGCTTTAATTTTCTTTCGGCAAATGAAACTCAGGTTAAAACCAAATTCTATTACCTTGGTTTCCGTCCTAGCTGCTTGTGCTAGAATAGGAGCTCTGATGTGTGGAAAGGAGATTCATGCTCATGCACTAAGGAGTGGTGTAGCATTTGATGGTTTTTTACCCAATGCACTTCTTGACATGTATGTAAGGTGTGGAAGGATGGGATCCGCATGGAACCAGTTCAACCATAACAAGAACGATGTCACTGCATGGAATATCATACTAACTGGGTATGCCCAGAGGGGGCAAGGAAGGAATGCCATTGAGATATTCCACACAATGGCTGAGTCAAGGGTAGACCCAGATGAGATCACATTTATGGCGCTACTATGTGCTTGTAGTAGGTCTGGTATGGTTAGTGAAGGTTTGGAGTATTTCATTAACATGAAACTTAATTATGGCATTGCACCTAATCTGAAACACTATGCCTGCATTGTCGATCTACTTGGCCGCGCTGGGCAATTGGCTGAAGCTCATGAGTTTATACAGAAGATGCCTATAAATCCAGATGCAGCTATCTGGGGAGCCTTGTTAAATGCTTGTGTGATCCACAGGCAGGTTGAACTTGGTGAACTTGCAGCCCAGAAAATATTTGAATTGGATGCCCAAAGTGTAGGGTATTATATGCTTATCTGTAACCTTTATGCAGACAGTGGTATGTGGGAAGAAGTTGCAGTGGTAAGAAACATGATGAGGGAGAGGGGCCTAACTGTAGATCCCGGGTGCAGTTGGGTTGAGGTAAAGGGAAAAGTTCATGCTTTCCTAAGTGGCGATAACTTCCACCCTCAAATAGAGGAACTTAATGCAGTTTTGGAGGGATTTTATGAGAAAATGAGATCATTGGCTTTCACAGAGCCAGAAAGTAGGCTTAAAGATGAAGGTGAAGCTTCAAAAGCTGAGATCTTTTGTGGACACAGTGAGAGACTAGCAGTTGCATTTGGCCTCATTAATACAGCCCCGGGGATGCCTATATGGGTGACAAAGAATCTTTATATGTGCCAAAGCTGTCACAGTACTGTCAAATTTATCTCTAAGGTTGTGCGCCGGGAGATTTGTGTAAGGGATACTGAAAAATTCCACCATTTTAAGGATGGCTTCTGTACTTGTGGGGATGAAGGTTATTGGGGAAAGCCAGATAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009451 GO:0009507 GO:0009536 GO:0009628 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

879

Amino Acids

98.52

Weight (kDa)

6.06

Isoelectric Point (pI)

33.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	163 - 210	2.4e-09	PPR repeat family
PPR	PF01535	166 - 195	7.8e-07	PPR repeat
PPR_2	PF13041	265 - 311	1.9e-08	PPR repeat family
PPR	PF01535	267 - 295	4.6e-06	PPR repeat
PPR_2	PF13041	365 - 413	3.9e-10	PPR repeat family
PPR_2	PF13041	566 - 613	3.5e-10	PPR repeat family
E_motif	PF20431	683 - 745	9.1e-18	E motif
DYW_deaminase	PF14432	789 - 870	5.4e-30	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0015938)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15510
fragaria_vesca	FvH4_6g30690
malus_domestica	MD17G1201500.v1.1
prunus_persica	Prupe.3G077400_v2.0.a1
pyrus_communis	pycom17g20540
rosa_chinensis	RchiOBHm_Chr2g0137061
rosa_laevigata	RLG00000019603
rosa_multiflora	Rmu_sc0001374.1_g000025
rosa_roxburghii	Rroxscaffold_2G00107710
rosa_rugosa	Rorug02G0337700
rosa_samantha	Rh2AG386800 Rh2BG393600 Rh2CG374100 Rh2DG409700
rosa_wichuraiana	Rw2G031650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2044
AccB7I	CCANNNNNTGG	1 cut(s)	1777
AccI	GTMKAC	2 cut(s)	602, 1794
AccII	CGCG	1 cut(s)	1950
AccIII	TCCGGA	1 cut(s)	895
AciI	CCGC	6 cut(s)	5, 226, 877, 1064, 1665, 1948
AclWI	GGATC	6 cut(s)	887, 1381, 1656, 1669, 2038, 2207
AcoI	YGGCCR	1 cut(s)	1945
AcsI	RAATTY	5 cut(s)	27, 379, 1501, 2519, 2570
AcuI	CTGAAG	2 cut(s)	272, 1986
AfaI	GTAC	3 cut(s)	365, 2512, 2599
AfiI	CCNNNNNNNGG	4 cut(s)	1740, 1777, 2053, 2584
AflIII	ACRYGT	1 cut(s)	1635
AgsI	TTSAA	7 cut(s)	556, 985, 1419, 1681, 2060, 2093, 2388
AjnI	CCWGG	2 cut(s)	1079, 1390
AjuI	GAANNNNNNNTTGG	2 cut(s)	977, 1009
AloI	GAACNNNNNNTCC	2 cut(s)	188, 220
Alw21I	GWGCWC	3 cut(s)	222, 763, 1552
Alw26I	GTCTC	3 cut(s)	786, 1012, 2413
Alw44I	GTGCAC	1 cut(s)	759
AlwI	GGATC	6 cut(s)	887, 1381, 1656, 1669, 2038, 2207
Ama87I	CYCGRG	3 cut(s)	239, 2216, 2455
Aor13HI	TCCGGA	1 cut(s)	895
AoxI	GGCC	4 cut(s)	1429, 1945, 2200, 2437
ApaLI	GTGCAC	1 cut(s)	759
ApeKI	GCWGC	4 cut(s)	156, 1529, 2009, 2075
ApoI	RAATTY	5 cut(s)	27, 379, 1501, 2519, 2570
AseI	ATTAAT	1 cut(s)	2445
Asp700I	GAANNNNTTC	1 cut(s)	1676
AspA2I	CCTAGG	1 cut(s)	785
AspLEI	GCGC	3 cut(s)	1822, 1952, 2541
AspS9I	GGNCC	1 cut(s)	2200
AsuC2I	CCSGG	6 cut(s)	535, 2217, 2218, 2456, 2457, 2543
AsuHPI	GGTGA	6 cut(s)	163, 494, 956, 2078, 2390, 2485
AvaI	CYCGRG	3 cut(s)	239, 2216, 2455
AvrII	CCTAGG	1 cut(s)	785
BaeGI	GKGCMC	1 cut(s)	763
BamHI	GGATCC	1 cut(s)	1661
BanII	GRGCYC	4 cut(s)	222, 358, 1299, 1552
BauI	CACGAG	2 cut(s)	344, 357
Bbv12I	GWGCWC	3 cut(s)	222, 763, 1552
BbvI	GCAGC	4 cut(s)	168, 1516, 2021, 2087
BccI	CCATC	6 cut(s)	344, 1078, 1171, 1601, 1651, 2582
BcgI	CGANNNNNNTGC	6 cut(s)	662, 696, 801, 835, 1685, 1719
BciT130I	CCWGG	2 cut(s)	1081, 1392
BciVI	GTATCC	1 cut(s)	2554
BcnI	CCSGG	6 cut(s)	535, 2217, 2218, 2456, 2457, 2543
BcoDI	GTCTC	3 cut(s)	786, 1012, 2413
BfaI	CTAG	6 cut(s)	129, 786, 915, 1526, 1539, 2423
BfmI	CTRYAG	3 cut(s)	154, 1212, 2208
BfoI	RGCGCY	1 cut(s)	1823
BfuAI	ACCTGC	1 cut(s)	2044
BfuI	GTATCC	1 cut(s)	2554
BglII	AGATCT	1 cut(s)	2397
BisI	GCNGC	6 cut(s)	157, 226, 1530, 1948, 2010, 2076
BlnI	CCTAGG	1 cut(s)	785
BlsI	GCNGC	6 cut(s)	158, 227, 1531, 1949, 2011, 2077
BmcAI	AGTACT	1 cut(s)	2512
Bme1390I	CCNGG	8 cut(s)	535, 1081, 1392, 2217, 2218, 2456, 2457, 2543
BmeT110I	CYCGRG	3 cut(s)	239, 2216, 2455
BmgT120I	GGNCC	1 cut(s)	2200
BmiI	GGNNCC	5 cut(s)	236, 1663, 1673, 2023, 2201
BmrFI	CCNGG	8 cut(s)	535, 1081, 1392, 2217, 2218, 2456, 2457, 2543
BmrI	ACTGGG	2 cut(s)	1302, 1734
BmsI	GCATC	7 cut(s)	289, 445, 864, 1980, 1996, 2089, 2451
BmuI	ACTGGG	2 cut(s)	1302, 1734
Bpu10I	CCTNAGC	1 cut(s)	89
BpuMI	CCSGG	6 cut(s)	535, 2217, 2218, 2456, 2457, 2543
Bsa29I	ATCGAT	1 cut(s)	1115
BsaJI	CCNNGG	8 cut(s)	229, 785, 1390, 1512, 2216, 2454, 2455, 2456
BsaWI	WCCGGW	1 cut(s)	895
BsaXI	ACNNNNNCTCC	4 cut(s)	188, 218, 466, 496
Bsc4I	CCNNNNNNNGG	4 cut(s)	1740, 1777, 2053, 2584
Bse1I	ACTGG	4 cut(s)	1218, 1297, 1675, 1729
Bse3DI	GCAATG	2 cut(s)	819, 1899
BseAI	TCCGGA	1 cut(s)	895
BseBI	CCWGG	2 cut(s)	1081, 1392
BseCI	ATCGAT	1 cut(s)	1115
BseDI	CCNNGG	8 cut(s)	229, 785, 1390, 1512, 2216, 2454, 2455, 2456
BseGI	GGATG	9 cut(s)	355, 496, 706, 1089, 1662, 2104, 2466, 2593, 2614
BseLI	CCNNNNNNNGG	4 cut(s)	1740, 1777, 2053, 2584
BseMI	GCAATG	2 cut(s)	819, 1899
BseMII	CTCAG	7 cut(s)	71, 634, 848, 1140, 1501, 1773, 2385
BseNI	ACTGG	4 cut(s)	1218, 1297, 1675, 1729
BseRI	GAGGAG	1 cut(s)	154
BseSI	GKGCMC	1 cut(s)	763
BseXI	GCAGC	4 cut(s)	168, 1516, 2021, 2087
BseYI	CCCAGC	1 cut(s)	1952
BsgI	GTGCAG	1 cut(s)	2242
Bsh1236I	CGCG	1 cut(s)	1950
BshFI	GGCC	4 cut(s)	1431, 1947, 2202, 2439
BshVI	ATCGAT	1 cut(s)	1115
BsiHKAI	GWGCWC	3 cut(s)	222, 763, 1552
BsiHKCI	CYCGRG	3 cut(s)	239, 2216, 2455
BsiSI	CCGG	6 cut(s)	534, 641, 896, 2217, 2456, 2542
BslFI	GGGAC	1 cut(s)	99
BslI	CCNNNNNNNGG	4 cut(s)	1740, 1777, 2053, 2584
BsmAI	GTCTC	3 cut(s)	786, 1012, 2413
BsmFI	GGGAC	1 cut(s)	99
BsmI	GAATGC	4 cut(s)	721, 786, 814, 1759
BsnI	GGCC	4 cut(s)	1431, 1947, 2202, 2439
BsoBI	CYCGRG	3 cut(s)	239, 2216, 2455
Bsp1286I	GDGCHC	5 cut(s)	222, 358, 763, 1299, 1552
Bsp13I	TCCGGA	1 cut(s)	895
Bsp1407I	TGTACA	1 cut(s)	363
Bsp143I	GATC	9 cut(s)	892, 1386, 1661, 1806, 1936, 2043, 2212, 2333, 2397
Bsp19I	CCATGG	1 cut(s)	229
BspACI	CCGC	6 cut(s)	5, 226, 877, 1064, 1665, 1948
BspANI	GGCC	4 cut(s)	1431, 1947, 2202, 2439
BspCNI	CTCAG	7 cut(s)	70, 633, 849, 1141, 1500, 1774, 2386
BspDI	ATCGAT	1 cut(s)	1115
BspEI	TCCGGA	1 cut(s)	895
BspFNI	CGCG	1 cut(s)	1950
BspHI	TCATGA	3 cut(s)	870, 1273, 1972
BspLI	GGNNCC	5 cut(s)	236, 1663, 1673, 2023, 2201
BspMAI	CTGCAG	1 cut(s)	158
BspMI	ACCTGC	1 cut(s)	2044
BspPI	GGATC	6 cut(s)	887, 1381, 1656, 1669, 2038, 2207
BsrDI	GCAATG	2 cut(s)	819, 1899
BsrGI	TGTACA	1 cut(s)	363
BsrI	ACTGG	4 cut(s)	1218, 1297, 1675, 1729
BssECI	CCNNGG	8 cut(s)	229, 785, 1390, 1512, 2216, 2454, 2455, 2456
BssMI	GATC	9 cut(s)	892, 1386, 1661, 1806, 1936, 2043, 2212, 2333, 2397
BssNAI	GTATAC	1 cut(s)	603
BssSI	CACGAG	2 cut(s)	344, 357
BssT1I	CCWWGG	3 cut(s)	229, 785, 1512
Bst1107I	GTATAC	1 cut(s)	603
Bst2BI	CACGAG	2 cut(s)	344, 357
Bst2UI	CCWGG	2 cut(s)	1081, 1392
Bst4CI	ACNGT	7 cut(s)	151, 910, 2150, 2209, 2414, 2510, 2515
Bst6I	CTCTTC	1 cut(s)	329
BstAUI	TGTACA	1 cut(s)	363
BstC8I	GCNNGC	3 cut(s)	931, 1281, 1927
BstDSI	CCRYGG	1 cut(s)	229
BstF5I	GGATG	9 cut(s)	355, 496, 706, 1089, 1662, 2104, 2466, 2593, 2614
BstFNI	CGCG	1 cut(s)	1950
BstH2I	RGCGCY	1 cut(s)	1823
BstHHI	GCGC	3 cut(s)	1822, 1952, 2541
BstKTI	GATC	9 cut(s)	895, 1389, 1664, 1809, 1939, 2046, 2215, 2336, 2400
BstMAI	GTCTC	3 cut(s)	786, 1012, 2413
BstMBI	GATC	9 cut(s)	892, 1386, 1661, 1806, 1936, 2043, 2212, 2333, 2397
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 308, 1276, 1535, 2390
BstNI	CCWGG	2 cut(s)	1081, 1392
BstNSI	RCATGY	3 cut(s)	430, 677, 1639
BstSCI	CCNGG	8 cut(s)	533, 1079, 1390, 2215, 2216, 2454, 2455, 2541
BstSFI	CTRYAG	3 cut(s)	154, 1212, 2208
BstSLI	GKGCMC	1 cut(s)	763
BstUI	CGCG	1 cut(s)	1950
BstV1I	GCAGC	4 cut(s)	168, 1516, 2021, 2087
BstX2I	RGATCY	4 cut(s)	1386, 1661, 2212, 2397
BstYI	RGATCY	4 cut(s)	1386, 1661, 2212, 2397
BstZ17I	GTATAC	1 cut(s)	603
Bsu15I	ATCGAT	1 cut(s)	1115
BsuI	GTATCC	1 cut(s)	2554
BsuRI	GGCC	4 cut(s)	1431, 1947, 2202, 2439
BsuTUI	ATCGAT	1 cut(s)	1115
BtgI	CCRYGG	1 cut(s)	229
BtsCI	GGATG	9 cut(s)	355, 496, 706, 1089, 1662, 2104, 2466, 2593, 2614
BtsI	GCAGTG	3 cut(s)	1142, 1701, 2178
BtsIMutI	CAGTG	7 cut(s)	147, 1142, 1225, 1701, 2155, 2178, 2419
BveI	ACCTGC	1 cut(s)	2044
Cac8I	GCNNGC	3 cut(s)	931, 1281, 1927
CciI	TCATGA	3 cut(s)	870, 1273, 1972
CfoI	GCGC	3 cut(s)	1822, 1952, 2541
Cfr13I	GGNCC	1 cut(s)	2200
Cfr9I	CCCGGG	2 cut(s)	2216, 2455
ClaI	ATCGAT	1 cut(s)	1115
Csp6I	GTAC	3 cut(s)	364, 2511, 2598
CviQI	GTAC	3 cut(s)	364, 2511, 2598
DpnI	GATC	9 cut(s)	894, 1388, 1663, 1808, 1938, 2045, 2214, 2335, 2399
DpnII	GATC	9 cut(s)	892, 1386, 1661, 1806, 1936, 2043, 2212, 2333, 2397
EaeI	YGGCCR	1 cut(s)	1945
Eam1104I	CTCTTC	1 cut(s)	329
EarI	CTCTTC	1 cut(s)	329
Ecl136II	GAGCTC	2 cut(s)	220, 1550
Eco130I	CCWWGG	3 cut(s)	229, 785, 1512
Eco24I	GRGCYC	4 cut(s)	222, 358, 1299, 1552
Eco53kI	GAGCTC	2 cut(s)	220, 1550
Eco57I	CTGAAG	2 cut(s)	272, 1986
Eco88I	CYCGRG	3 cut(s)	239, 2216, 2455
EcoICRI	GAGCTC	2 cut(s)	220, 1550
EcoO109I	RGGNCCY	1 cut(s)	2200
EcoRI	GAATTC	1 cut(s)	379
EcoRII	CCWGG	2 cut(s)	1079, 1390
EcoT14I	CCWWGG	3 cut(s)	229, 785, 1512
EcoT22I	ATGCAT	1 cut(s)	1358
EcoT38I	GRGCYC	4 cut(s)	222, 358, 1299, 1552
ErhI	CCWWGG	3 cut(s)	229, 785, 1512
FalI	AAGNNNNNCTT	2 cut(s)	2576, 2608
FaqI	GGGAC	1 cut(s)	99
FauI	CCCGC	1 cut(s)	870
FblI	GTMKAC	2 cut(s)	602, 1794
Fnu4HI	GCNGC	6 cut(s)	157, 226, 1530, 1948, 2010, 2076
FokI	GGATG	9 cut(s)	362, 503, 713, 1096, 1669, 2111, 2473, 2600, 2621
FriOI	GRGCYC	4 cut(s)	222, 358, 1299, 1552
Fsp4HI	GCNGC	6 cut(s)	157, 226, 1530, 1948, 2010, 2076
FspBI	CTAG	6 cut(s)	129, 786, 915, 1526, 1539, 2423
GlaI	GCGC	3 cut(s)	1821, 1951, 2540
GluI	GCNGC	6 cut(s)	157, 226, 1530, 1948, 2010, 2076
GsaI	CCCAGC	1 cut(s)	1956
HaeII	RGCGCY	1 cut(s)	1823
HaeIII	GGCC	4 cut(s)	1431, 1947, 2202, 2439
HapII	CCGG	6 cut(s)	534, 641, 896, 2217, 2456, 2542
HhaI	GCGC	3 cut(s)	1822, 1952, 2541
Hin6I	GCGC	3 cut(s)	1820, 1950, 2539
HinP1I	GCGC	3 cut(s)	1820, 1950, 2539
HindIII	AAGCTT	3 cut(s)	91, 1268, 2382
HinfI	GANTC	8 cut(s)	263, 664, 679, 695, 1027, 1570, 1784, 2482
HpaII	CCGG	6 cut(s)	534, 641, 896, 2217, 2456, 2542
HphI	GGTGA	6 cut(s)	163, 494, 956, 2078, 2390, 2485
Hpy166II	GTNNAC	7 cut(s)	364, 603, 761, 1000, 1795, 2069, 2408
Hpy188I	TCNGA	8 cut(s)	208, 252, 623, 700, 1032, 1437, 1554, 1914
Hpy188III	TCNNGA	8 cut(s)	129, 241, 871, 896, 1274, 1631, 1973, 2003
Hpy8I	GTNNAC	7 cut(s)	364, 603, 761, 1000, 1795, 2069, 2408
HpyAV	CCTTC	8 cut(s)	845, 1147, 1442, 1647, 1743, 1853, 2369, 2606
HpyCH4III	ACNGT	7 cut(s)	151, 910, 2150, 2209, 2414, 2510, 2515
HpyCH4IV	ACGT	1 cut(s)	605
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 308, 1276, 1535, 2390
HpySE526I	ACGT	1 cut(s)	605
HspAI	GCGC	3 cut(s)	1820, 1950, 2539
Kpn2I	TCCGGA	1 cut(s)	895
Kzo9I	GATC	9 cut(s)	892, 1386, 1661, 1806, 1936, 2043, 2212, 2333, 2397
LmnI	GCTCC	4 cut(s)	111, 1180, 1547, 2021
Lsp1109I	GCAGC	4 cut(s)	168, 1516, 2021, 2087
LweI	GCATC	7 cut(s)	289, 445, 864, 1980, 1996, 2089, 2451
MaeI	CTAG	6 cut(s)	129, 786, 915, 1526, 1539, 2423
MaeII	ACGT	1 cut(s)	605
MaeIII	GTNAC	6 cut(s)	169, 910, 1699, 2132, 2473, 2504
MalI	GATC	9 cut(s)	894, 1388, 1663, 1808, 1938, 2045, 2214, 2335, 2399
MboI	GATC	9 cut(s)	892, 1386, 1661, 1806, 1936, 2043, 2212, 2333, 2397
MboII	GAAGA	7 cut(s)	117, 305, 308, 346, 1100, 1999, 2174
MfeI	CAATTG	1 cut(s)	1958
MflI	RGATCY	4 cut(s)	1386, 1661, 2212, 2397
MhlI	GDGCHC	5 cut(s)	222, 358, 763, 1299, 1552
MlyI	GAGTC	3 cut(s)	257, 704, 1793
MmeI	TCCRAC	2 cut(s)	231, 678
Mph1103I	ATGCAT	1 cut(s)	1358
MroI	TCCGGA	1 cut(s)	895
MroXI	GAANNNNTTC	1 cut(s)	1676
MslI	CAYNNNNRTG	8 cut(s)	348, 431, 672, 975, 1334, 1578, 1814, 1880
MspI	CCGG	6 cut(s)	534, 641, 896, 2217, 2456, 2542
MspR9I	CCNGG	8 cut(s)	535, 1081, 1392, 2217, 2218, 2456, 2457, 2543
MunI	CAATTG	1 cut(s)	1958
Mva1269I	GAATGC	4 cut(s)	721, 786, 814, 1759
MvaI	CCWGG	2 cut(s)	1081, 1392
MvnI	CGCG	1 cut(s)	1950
MwoI	GCNNNNNNNGC	5 cut(s)	11, 308, 1276, 1535, 2390
NciI	CCSGG	6 cut(s)	535, 2217, 2218, 2456, 2457, 2543
NcoI	CCATGG	1 cut(s)	229
NdeII	GATC	9 cut(s)	892, 1386, 1661, 1806, 1936, 2043, 2212, 2333, 2397
NlaIV	GGNNCC	5 cut(s)	236, 1663, 1673, 2023, 2201
NmeAIII	GCCGAG	1 cut(s)	1407
NmuCI	GTSAC	5 cut(s)	169, 910, 1699, 2473, 2504
NsiI	ATGCAT	1 cut(s)	1358
NspI	RCATGY	3 cut(s)	430, 677, 1639
PaeR7I	CTCGAG	1 cut(s)	239
PagI	TCATGA	3 cut(s)	870, 1273, 1972
PciI	ACATGT	1 cut(s)	1635
PctI	GAATGC	4 cut(s)	721, 786, 814, 1759
PdmI	GAANNNNTTC	1 cut(s)	1676
PfeI	GAWTC	5 cut(s)	664, 679, 1027, 1570, 2482
PflFI	GACNNNGTC	1 cut(s)	1004
PflMI	CCANNNNNTGG	1 cut(s)	1777
PfoI	TCCNGGA	1 cut(s)	1079
PkrI	GCNGC	6 cut(s)	158, 227, 1531, 1949, 2011, 2077
PleI	GAGTC	3 cut(s)	257, 703, 1792
PpsI	GAGTC	3 cut(s)	257, 703, 1792
PscI	ACATGT	1 cut(s)	1635
PshBI	ATTAAT	1 cut(s)	2445
Psp124BI	GAGCTC	2 cut(s)	222, 1552
Psp6I	CCWGG	2 cut(s)	1079, 1390
PspFI	CCCAGC	1 cut(s)	1952
PspGI	CCWGG	2 cut(s)	1079, 1390
PspN4I	GGNNCC	5 cut(s)	236, 1663, 1673, 2023, 2201
PspPI	GGNCC	1 cut(s)	2200
PstI	CTGCAG	1 cut(s)	158
PsuI	RGATCY	4 cut(s)	1386, 1661, 2212, 2397
PsyI	GACNNNGTC	1 cut(s)	1004
RsaI	GTAC	3 cut(s)	365, 2512, 2599
RsaNI	GTAC	3 cut(s)	364, 2511, 2598
RseI	CAYNNNNRTG	8 cut(s)	348, 431, 672, 975, 1334, 1578, 1814, 1880
SacI	GAGCTC	2 cut(s)	222, 1552
SatI	GCNGC	6 cut(s)	157, 226, 1530, 1948, 2010, 2076
Sau3AI	GATC	9 cut(s)	892, 1386, 1661, 1806, 1936, 2043, 2212, 2333, 2397
Sau96I	GGNCC	1 cut(s)	2200
ScaI	AGTACT	1 cut(s)	2512
SchI	GAGTC	3 cut(s)	257, 704, 1793
ScrFI	CCNGG	8 cut(s)	535, 1081, 1392, 2217, 2218, 2456, 2457, 2543
SduI	GDGCHC	5 cut(s)	222, 358, 763, 1299, 1552
SfaNI	GCATC	7 cut(s)	289, 445, 864, 1980, 1996, 2089, 2451
SfcI	CTRYAG	3 cut(s)	154, 1212, 2208
Sfr274I	CTCGAG	1 cut(s)	239
SlaI	CTCGAG	1 cut(s)	239
SmaI	CCCGGG	2 cut(s)	2218, 2457
SmiMI	CAYNNNNRTG	8 cut(s)	348, 431, 672, 975, 1334, 1578, 1814, 1880
SmlI	CTYRAG	1 cut(s)	239
SmoI	CTYRAG	1 cut(s)	239
SsiI	CCGC	6 cut(s)	5, 226, 877, 1064, 1665, 1948
SspI	AATATT	1 cut(s)	2088
SspMI	CTAG	6 cut(s)	129, 786, 915, 1526, 1539, 2423
SstI	GAGCTC	2 cut(s)	222, 1552
StyD4I	CCNGG	8 cut(s)	533, 1079, 1390, 2215, 2216, 2454, 2455, 2541
StyI	CCWWGG	3 cut(s)	229, 785, 1512
TaaI	ACNGT	7 cut(s)	151, 910, 2150, 2209, 2414, 2510, 2515
TaiI	ACGT	1 cut(s)	608
TatI	WGTACW	3 cut(s)	363, 2510, 2597
TauI	GCSGC	2 cut(s)	228, 1950
TfiI	GAWTC	5 cut(s)	664, 679, 1027, 1570, 2482
TscAI	CASTG	7 cut(s)	154, 1149, 1225, 1708, 2155, 2178, 2419
TseFI	GTSAC	5 cut(s)	169, 910, 1699, 2473, 2504
TseI	GCWGC	4 cut(s)	156, 1529, 2009, 2075
Tsp45I	GTSAC	5 cut(s)	169, 910, 1699, 2473, 2504
TspGWI	ACGGA	3 cut(s)	157, 1004, 1510
TspMI	CCCGGG	2 cut(s)	2216, 2455
TspRI	CASTG	7 cut(s)	154, 1149, 1225, 1708, 2155, 2178, 2419
Tth111I	GACNNNGTC	1 cut(s)	1004
Van91I	CCANNNNNTGG	1 cut(s)	1777
VneI	GTGCAC	1 cut(s)	759
VspI	ATTAAT	1 cut(s)	2445
XapI	RAATTY	5 cut(s)	27, 379, 1501, 2519, 2570
XbaI	TCTAGA	1 cut(s)	128
XceI	RCATGY	3 cut(s)	430, 677, 1639
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2585
XhoI	CTCGAG	1 cut(s)	239
XmaI	CCCGGG	2 cut(s)	2216, 2455
XmaJI	CCTAGG	1 cut(s)	785
XmiI	GTMKAC	2 cut(s)	602, 1794
XmnI	GAANNNNTTC	1 cut(s)	1676
XspI	CTAG	6 cut(s)	129, 786, 915, 1526, 1539, 2423
ZrmI	AGTACT	1 cut(s)	2512
Zsp2I	ATGCAT	1 cut(s)	1358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.