Rh2BG661300

Mitochondrial inner membrane protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018575
Physical Location & Seq
Reverse (-)
87633093 .. 87635913
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG661300.1

Sequence Viewer

Length: 483 bp
ATGCAGGTGGTGGCAGGCAGCACTGTGGTGCGATCCTGTCAAGGCGATCCAAGGGGAGGTGTGGTTGGCCAGTCGGGCGACCCGTTCTTCCTCTGGTCTCGAGTTGATGATGGTTGTGCTGATGGTGTTGACTGGTATTTGGCTGACCTAAGTTGGGCAGGGAGGATACTAGGCGATCTTGACGGCGATGGGAGCTTCGCCGCCTTGTTGGGATGCGGGTTGGTGCGACGACGAGCGTCTGGTGTTGCTGCTCCGGCGACGAGATTCAATCCCAGAGTAGGTGTATGTAATGTCCTAAACCAAACCCCAGTTGCATCTGTTGACACAAAGGAGTCTTTATATGCCCTGGATTGCGTGAGACGAAGAGTTATGAAATCGGTCACTGCTAATCCCTACTGCTCAGAAGCAGCAGCAAAGGATGCCATGGAAGCTGTGTGGGATGTTTGTTACAATGATACGCAGCCCTTTGACAGAGCCCCTTGA

Protein Analysis

160

Amino Acids

17.07

Weight (kDa)

4.86

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M76 PF09768 94 - 157 1.1e-13 Peptidase M76 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018575)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0134621
rosa_laevigata RLG00000017936
rosa_samantha Rh1AG309800 Rh1CG265600 Rh1CG281900 Rh2BG661300 Rh6AG094900 Rh6BG500900 Rh7AG075000
rosa_wichuraiana Rw2G030530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 201, 216
AclWI GGATC 2 cut(s) 27, 41
AcoI YGGCCR 1 cut(s) 67
AfiI CCNNNNNNNGG 3 cut(s) 56, 154, 278
AgsI TTSAA 1 cut(s) 268
AjnI CCWGG 1 cut(s) 345
AleI CACNNNNGTG 1 cut(s) 26
AluBI AGCT 2 cut(s) 195, 431
AluI AGCT 2 cut(s) 195, 431
Alw26I GTCTC 2 cut(s) 102, 352
AlwI GGATC 2 cut(s) 27, 41
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 1 cut(s) 67
ApeKI GCWGC 5 cut(s) 18, 248, 407, 410, 460
AvaI CYCGRG 1 cut(s) 99
BalI TGGCCA 1 cut(s) 69
BanII GRGCYC 1 cut(s) 478
BbvI GCAGC 5 cut(s) 30, 235, 419, 422, 472
BccI CCATC 3 cut(s) 104, 116, 182
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 1 cut(s) 347
BciVI GTATCC 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 102, 352
BfaI CTAG 1 cut(s) 170
BfuI GTATCC 1 cut(s) 159
BglI GCCNNNNNGGC 1 cut(s) 75
BisI GCNGC 6 cut(s) 19, 201, 249, 408, 411, 461
BlsI GCNGC 6 cut(s) 20, 202, 250, 409, 412, 462
Bme1390I CCNGG 1 cut(s) 347
BmeT110I CYCGRG 1 cut(s) 99
BmrFI CCNGG 1 cut(s) 347
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 3 cut(s) 203, 323, 409
BmuI ACTGGG 1 cut(s) 302
BoxI GACNNNNGTC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 50, 345, 423
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 154, 278
Bse1I ACTGG 3 cut(s) 70, 137, 308
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 3 cut(s) 50, 345, 423
BseGI GGATG 3 cut(s) 218, 424, 445
BseLI CCNNNNNNNGG 3 cut(s) 56, 154, 278
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 3 cut(s) 70, 137, 308
BseXI GCAGC 5 cut(s) 30, 235, 419, 422, 472
BshFI GGCC 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 3 cut(s) 56, 154, 278
BsmAI GTCTC 2 cut(s) 102, 352
BsmBI CGTCTC 1 cut(s) 352
BsnI GGCC 1 cut(s) 69
Bso31I GGTCTC 1 cut(s) 102
BsoBI CYCGRG 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 478
Bsp143I GATC 3 cut(s) 32, 46, 175
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 2 cut(s) 201, 216
BspANI GGCC 1 cut(s) 69
BspCNI CTCAG 1 cut(s) 413
BspPI GGATC 2 cut(s) 27, 41
BspTNI GGTCTC 1 cut(s) 102
BsrI ACTGG 3 cut(s) 70, 137, 308
BssECI CCNNGG 3 cut(s) 50, 345, 423
BssMI GATC 3 cut(s) 32, 46, 175
BssT1I CCWWGG 2 cut(s) 50, 423
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 358
BstAPI GCANNNNNTGC 1 cut(s) 419
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 2 cut(s) 149, 400
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 3 cut(s) 218, 424, 445
BstKTI GATC 3 cut(s) 35, 49, 178
BstMAI GTCTC 2 cut(s) 102, 352
BstMBI GATC 3 cut(s) 32, 46, 175
BstMWI GCNNNNNNNGC 5 cut(s) 75, 192, 254, 419, 428
BstNI CCWGG 1 cut(s) 347
BstPAI GACNNNNGTC 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 345
BstV1I GCAGC 5 cut(s) 30, 235, 419, 422, 472
BsuI GTATCC 1 cut(s) 159
BsuRI GGCC 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 423
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 3 cut(s) 218, 424, 445
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 21, 381
Cac8I GCNNGC 1 cut(s) 16
CseI GACGC 1 cut(s) 225
CviAII CATG 1 cut(s) 424
CviJI RGCY 6 cut(s) 69, 143, 195, 431, 463, 476
CviKI_1 RGCY 6 cut(s) 69, 143, 195, 431, 463, 476
DdeI CTNAG 2 cut(s) 149, 400
DpnI GATC 3 cut(s) 34, 48, 177
DpnII GATC 3 cut(s) 32, 46, 175
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Eco130I CCWWGG 2 cut(s) 50, 423
Eco24I GRGCYC 1 cut(s) 478
Eco31I GGTCTC 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 345
EcoT14I CCWWGG 2 cut(s) 50, 423
EcoT38I GRGCYC 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 50, 423
Esp3I CGTCTC 1 cut(s) 352
FaeI CATG 1 cut(s) 427
FaiI YATR 5 cut(s) 286, 340, 342, 371, 425
FatI CATG 1 cut(s) 423
FauI CCCGC 1 cut(s) 209
Fnu4HI GCNGC 6 cut(s) 19, 201, 249, 408, 411, 461
FokI GGATG 3 cut(s) 225, 431, 452
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 6 cut(s) 19, 201, 249, 408, 411, 461
FspBI CTAG 1 cut(s) 170
GluI GCNGC 6 cut(s) 19, 201, 249, 408, 411, 461
HaeIII GGCC 1 cut(s) 69
HapII CCGG 1 cut(s) 254
HgaI GACGC 1 cut(s) 225
Hin1II CATG 1 cut(s) 427
HincII GTYRAC 2 cut(s) 130, 322
HindII GTYRAC 2 cut(s) 130, 322
HinfI GANTC 2 cut(s) 264, 332
HpaII CCGG 1 cut(s) 254
Hpy166II GTNNAC 2 cut(s) 130, 322
Hpy188I TCNGA 1 cut(s) 403
Hpy188III TCNNGA 2 cut(s) 99, 179
Hpy8I GTNNAC 2 cut(s) 130, 322
Hpy99I CGWCG 3 cut(s) 231, 234, 262
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 2 cut(s) 4, 314
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 192, 254, 419, 428
HpyF3I CTNAG 2 cut(s) 149, 400
Hsp92II CATG 1 cut(s) 427
Kzo9I GATC 3 cut(s) 32, 46, 175
LmnI GCTCC 2 cut(s) 192, 256
Lsp1109I GCAGC 5 cut(s) 30, 235, 419, 422, 472
LweI GCATC 3 cut(s) 203, 323, 409
MaeI CTAG 1 cut(s) 170
MaeIII GTNAC 2 cut(s) 379, 446
MalI GATC 3 cut(s) 34, 48, 177
MboI GATC 3 cut(s) 32, 46, 175
MboII GAAGA 2 cut(s) 79, 375
MhlI GDGCHC 1 cut(s) 478
MlsI TGGCCA 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 69
MlyI GAGTC 1 cut(s) 341
MnlI CCTC 3 cut(s) 50, 101, 156
Mox20I TGGCCA 1 cut(s) 69
MscI TGGCCA 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 26
Msp20I TGGCCA 1 cut(s) 69
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 347
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 5 cut(s) 75, 192, 254, 419, 428
NcoI CCATGG 1 cut(s) 423
NdeII GATC 3 cut(s) 32, 46, 175
NlaIII CATG 1 cut(s) 427
NmuCI GTSAC 1 cut(s) 379
OliI CACNNNNGTG 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PfeI GAWTC 1 cut(s) 264
PkrI GCNGC 6 cut(s) 20, 202, 250, 409, 412, 462
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
PshAI GACNNNNGTC 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
RseI CAYNNNNRTG 1 cut(s) 26
SatI GCNGC 6 cut(s) 19, 201, 249, 408, 411, 461
Sau3AI GATC 3 cut(s) 32, 46, 175
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 1 cut(s) 478
SetI ASST 6 cut(s) 9, 61, 150, 197, 283, 433
SfaNI GCATC 3 cut(s) 203, 323, 409
Sfr274I CTCGAG 1 cut(s) 99
SlaI CTCGAG 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
SsiI CCGC 2 cut(s) 201, 216
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 345
StyI CCWWGG 2 cut(s) 50, 423
TaaI ACNGT 1 cut(s) 25
TaqI TCGA 1 cut(s) 100
TaqII GACCGA 1 cut(s) 367
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 1 cut(s) 264
TscAI CASTG 2 cut(s) 28, 388
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 5 cut(s) 18, 248, 407, 410, 460
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 1 cut(s) 386
TspRI CASTG 2 cut(s) 28, 388
XhoI CTCGAG 1 cut(s) 99
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.