Rh2DG591500

proline-rich domain in spliceosome associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007074
Physical Location & Seq
Reverse (-)
82413969 .. 82418301
4333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG591500.1

Sequence Viewer

Length: 1353 bp
ATGATCTTCGTTAATGTTCTCCATAATTCAACATCATCAGCTAAAACAGGTGTAAAGAGGGCTCGAACAACAGTTGACGAGCAGCAAGCTTCTGTCCGTGTAACATATAATTCTTTAACAAGAGCTAGTAAACACAAGCTTGAAGAACTATTACAGCAGTGGTCAGAATGGCATGCCAAACATGTTTCTTCCTCTCAAGATCCTACTCAAGTGTTAGAATCAGGTGAAGAGACGCTCTTTCCTGCTCTTCGTTTTGGCACAGAGAGGACGTCTGGGGTGTCTTTTTGGATGGACAACCAAACAGGGACAGCACAGAACAGGGAATCGATTCCTTTGGATAGCAACTATGCGCCTCTATATGATCGTGGCTATGCGTTAGGCTTGACTATGGCTGGTAGTTCAACTAATCAGGAGGGGGGCTTGGAGATTATAGATGATGCTAGCCGTTGTTTCAATTGTGGTTCTTACAATCATGCGTTGAAGGAATGCCCCAAGCCTCGTGACCATGTTGCTGTCAATAATGCCCGCAAACAGCGGAAGATAAAAAAAAATCAAACTCCTAATTCTCGCAACTCAACTCGATATTATCAGAATTCTCCAGCTGGAAAATACGATGGTCTAAGGCCCGGTGCTCTCCATGCTGACACAAGGAAGCTTTTGGGTCTTGGGGAACTTGATCCACCTCCTTGGCTTAACAGAATGCGAGAAATTGGATACCCACCAGGATATTTAGATGTAGATGATGAGGACCAGCCATCAGGAATAATTATACATGGTGGTGAGGAAACTAAGGTTGAACAGGAGGATGGGGAAATCATCGAAACAGACCATCCTGAACCATCTGAACCACAGAGGAAAATGACAGTCGACTTTCCAGGAGTAAATGCACCAATCCCAGAAAATGCTGATGAGAGACTCTGGGCACCCGGGCCTTCATTTTCAGATCCCTCAAGGAACCGCTCGCACAACAGGTCAAACCATTATTCTGAACCTGTCAGCAGAGGCCATTATCATGAGCAGAGGTGGTCTCGAGATTATAGAGATGATGGGCCTCCTGGTGTTGACCCAGGTTATGGGCCATCTAGTTATCCTCCGAGATATGGGAGTCATGATTACAGTTACAACTCCCGCAGTTCGAGTAATGCTTCAGCGCCTAGAAGCCCTACCTTTGGAAGGTCCCAAACAGACAGAGGCAGAAGAAGCAGTGAGTTTTCTTTTCCATACTCACCCACCAGGGATTCCAACCCCAAATATGATTATTTGAATGACGAAAGCAAGAATGACTATGACCCTGATAGTTCAAGTCATGGTTGGGATAGGGTAGATAGGCATCGCCATCACCGTAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PSP zf-CCHC

Protein Analysis

450

Amino Acids

50.95

Weight (kDa)

6.42

Isoelectric Point (pI)

61.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSP PF04046 208 - 245 2.7e-11 PSP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 922
AccB7I CCANNNNNTGG 1 cut(s) 1073
AccBSI CCGCTC 1 cut(s) 960
AccI GTMKAC 1 cut(s) 867
AciI CCGC 4 cut(s) 526, 535, 958, 1129
AclWI GGATC 3 cut(s) 194, 671, 938
AcsI RAATTY 1 cut(s) 592
AcuI CTGAAG 1 cut(s) 1131
AcyI GRCGYC 1 cut(s) 269
AfiI CCNNNNNNNGG 4 cut(s) 1073, 1100, 1169, 1173
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 8 cut(s) 30, 143, 402, 454, 481, 797, 1264, 1302
AjnI CCWGG 5 cut(s) 721, 874, 1054, 1066, 1232
AluBI AGCT 6 cut(s) 41, 89, 125, 139, 602, 655
AluI AGCT 6 cut(s) 41, 89, 125, 139, 602, 655
Alw21I GWGCWC 1 cut(s) 634
Alw26I GTCTC 3 cut(s) 224, 907, 1032
AlwI GGATC 3 cut(s) 194, 671, 938
Ama87I CYCGRG 2 cut(s) 926, 1029
AoxI GGCC 5 cut(s) 623, 929, 1003, 1049, 1076
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 1 cut(s) 592
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 2 cut(s) 352, 1153
AspS9I GGNCC 6 cut(s) 624, 748, 929, 1049, 1076, 1176
AsuC2I CCSGG 3 cut(s) 627, 927, 928
AsuHPI GGTGA 4 cut(s) 236, 791, 1218, 1331
AsuNHI GCTAGC 1 cut(s) 440
AvaI CYCGRG 2 cut(s) 926, 1029
AvaII GGWCC 2 cut(s) 748, 1176
BaeGI GKGCMC 1 cut(s) 925
BanI GGYRCC 1 cut(s) 922
BanII GRGCYC 1 cut(s) 64
BarI GAAGNNNNNNTAC 2 cut(s) 135, 167
BauI CACGAG 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 1 cut(s) 94
BccI CCATC 9 cut(s) 283, 608, 763, 800, 837, 847, 1040, 1087, 1344
BceAI ACGGC 1 cut(s) 429
BciT130I CCWGG 5 cut(s) 723, 876, 1056, 1068, 1234
BciVI GTATCC 1 cut(s) 707
BcnI CCSGG 3 cut(s) 627, 927, 928
BcoDI GTCTC 3 cut(s) 224, 907, 1032
BfaI CTAG 4 cut(s) 126, 441, 1083, 1155
BfoI RGCGCY 1 cut(s) 1154
BfuI GTATCC 1 cut(s) 707
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
Bme1390I CCNGG 8 cut(s) 627, 723, 876, 927, 928, 1056, 1068, 1234
Bme18I GGWCC 2 cut(s) 748, 1176
BmeT110I CYCGRG 2 cut(s) 926, 1029
BmgT120I GGNCC 6 cut(s) 624, 748, 929, 1049, 1076, 1176
BmiI GGNNCC 3 cut(s) 924, 956, 1178
BmrFI CCNGG 8 cut(s) 627, 723, 876, 927, 928, 1056, 1068, 1234
BmsI GCATC 2 cut(s) 427, 1339
BmtI GCTAGC 1 cut(s) 444
BplI GAGNNNNNCTC 2 cut(s) 1012, 1044
BpmI CTGGAG 1 cut(s) 582
BpuEI CTTGAG 3 cut(s) 180, 192, 934
BpuMI CCSGG 3 cut(s) 627, 927, 928
Bsa29I ATCGAT 1 cut(s) 326
BsaBI GATNNNNATC 1 cut(s) 1329
BsaHI GRCGYC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 1032
BsaJI CCNNGG 4 cut(s) 686, 926, 1066, 1233
Bsc4I CCNNNNNNNGG 4 cut(s) 1073, 1100, 1169, 1173
Bse8I GATNNNNATC 1 cut(s) 1329
BseBI CCWGG 5 cut(s) 723, 876, 1056, 1068, 1234
BseCI ATCGAT 1 cut(s) 326
BseDI CCNNGG 4 cut(s) 686, 926, 1066, 1233
BseGI GGATG 3 cut(s) 294, 811, 829
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 4 cut(s) 1073, 1100, 1169, 1173
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 1 cut(s) 94
BshFI GGCC 5 cut(s) 625, 931, 1005, 1051, 1078
BshNI GGYRCC 1 cut(s) 922
BshVI ATCGAT 1 cut(s) 326
BsiHKAI GWGCWC 1 cut(s) 634
BsiHKCI CYCGRG 2 cut(s) 926, 1029
BsiSI CCGG 2 cut(s) 627, 927
BslFI GGGAC 2 cut(s) 319, 1162
BslI CCNNNNNNNGG 4 cut(s) 1073, 1100, 1169, 1173
BsmAI GTCTC 3 cut(s) 224, 907, 1032
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 2 cut(s) 319, 1162
BsmI GAATGC 2 cut(s) 491, 705
BsnI GGCC 5 cut(s) 625, 931, 1005, 1051, 1078
Bso31I GGTCTC 1 cut(s) 1032
BsoBI CYCGRG 2 cut(s) 926, 1029
Bsp1286I GDGCHC 3 cut(s) 64, 634, 925
Bsp143I GATC 5 cut(s) 3, 199, 361, 676, 943
BspACI CCGC 4 cut(s) 526, 535, 958, 1129
BspANI GGCC 5 cut(s) 625, 931, 1005, 1051, 1078
BspDI ATCGAT 1 cut(s) 326
BspHI TCATGA 2 cut(s) 1012, 1108
BspLI GGNNCC 3 cut(s) 924, 956, 1178
BspOI GCTAGC 1 cut(s) 444
BspPI GGATC 3 cut(s) 194, 671, 938
BspQI GCTCTTC 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 922
BspTNI GGTCTC 1 cut(s) 1032
BsrBI CCGCTC 1 cut(s) 960
BssECI CCNNGG 4 cut(s) 686, 926, 1066, 1233
BssMI GATC 5 cut(s) 3, 199, 361, 676, 943
BssNI GRCGYC 1 cut(s) 269
BssSI CACGAG 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 686
Bst2BI CACGAG 1 cut(s) 498
Bst2UI CCWGG 5 cut(s) 723, 876, 1056, 1068, 1234
Bst4CI ACNGT 4 cut(s) 73, 865, 1118, 1343
Bst6I CTCTTC 2 cut(s) 222, 252
BstACI GRCGYC 1 cut(s) 269
BstC8I GCNNGC 5 cut(s) 87, 174, 442, 526, 962
BstDEI CTNAG 2 cut(s) 620, 789
BstENI CCTNNNNNAGG 1 cut(s) 1171
BstF5I GGATG 3 cut(s) 294, 811, 829
BstH2I RGCGCY 1 cut(s) 1154
BstHHI GCGC 2 cut(s) 352, 1153
BstKTI GATC 5 cut(s) 6, 202, 364, 679, 946
BstMAI GTCTC 3 cut(s) 224, 907, 1032
BstMBI GATC 5 cut(s) 3, 199, 361, 676, 943
BstMWI GCNNNNNNNGC 2 cut(s) 638, 1200
BstNI CCWGG 5 cut(s) 723, 876, 1056, 1068, 1234
BstNSI RCATGY 2 cut(s) 176, 185
BstSCI CCNGG 8 cut(s) 625, 721, 874, 925, 926, 1054, 1066, 1232
BstSLI GKGCMC 1 cut(s) 925
BstV1I GCAGC 1 cut(s) 94
BstX2I RGATCY 2 cut(s) 199, 943
BstXI CCANNNNNNTGG 1 cut(s) 687
BstYI RGATCY 2 cut(s) 199, 943
Bsu15I ATCGAT 1 cut(s) 326
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 5 cut(s) 625, 931, 1005, 1051, 1078
BsuTUI ATCGAT 1 cut(s) 326
BtgZI GCGATG 1 cut(s) 1316
BtsCI GGATG 3 cut(s) 294, 811, 829
BtsI GCAGTG 2 cut(s) 164, 1210
BtsIMutI CAGTG 2 cut(s) 164, 1210
Cac8I GCNNGC 5 cut(s) 87, 174, 442, 526, 962
CciI TCATGA 2 cut(s) 1012, 1108
CfoI GCGC 2 cut(s) 352, 1153
Cfr13I GGNCC 6 cut(s) 624, 748, 929, 1049, 1076, 1176
Cfr9I CCCGGG 1 cut(s) 926
ClaI ATCGAT 1 cut(s) 326
CseI GACGC 1 cut(s) 241
CviAII CATG 9 cut(s) 173, 182, 473, 506, 638, 773, 1013, 1109, 1307
DdeI CTNAG 2 cut(s) 620, 789
DpnI GATC 5 cut(s) 5, 201, 363, 678, 945
DpnII GATC 5 cut(s) 3, 199, 361, 676, 943
Eam1104I CTCTTC 2 cut(s) 222, 252
EarI CTCTTC 2 cut(s) 222, 252
Eco130I CCWWGG 1 cut(s) 686
Eco24I GRGCYC 1 cut(s) 64
Eco31I GGTCTC 1 cut(s) 1032
Eco47I GGWCC 2 cut(s) 748, 1176
Eco57I CTGAAG 1 cut(s) 1131
Eco88I CYCGRG 2 cut(s) 926, 1029
EcoNI CCTNNNNNAGG 1 cut(s) 1171
EcoO109I RGGNCCY 1 cut(s) 1176
EcoRI GAATTC 1 cut(s) 592
EcoRII CCWGG 5 cut(s) 721, 874, 1054, 1066, 1232
EcoT14I CCWWGG 1 cut(s) 686
EcoT38I GRGCYC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 686
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 9 cut(s) 176, 185, 476, 509, 641, 776, 1016, 1112, 1310
FaqI GGGAC 2 cut(s) 319, 1162
FatI CATG 9 cut(s) 172, 181, 472, 505, 637, 772, 1012, 1108, 1306
FauI CCCGC 2 cut(s) 533, 1136
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 1 cut(s) 83
FokI GGATG 3 cut(s) 301, 816, 818
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 4 cut(s) 126, 441, 1083, 1155
GlaI GCGC 2 cut(s) 351, 1152
GluI GCNGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 582
HaeII RGCGCY 1 cut(s) 1154
HaeIII GGCC 5 cut(s) 625, 931, 1005, 1051, 1078
HapII CCGG 2 cut(s) 627, 927
HgaI GACGC 1 cut(s) 241
HhaI GCGC 2 cut(s) 352, 1153
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 9 cut(s) 176, 185, 476, 509, 641, 776, 1016, 1112, 1310
Hin6I GCGC 2 cut(s) 350, 1151
HinP1I GCGC 2 cut(s) 350, 1151
HincII GTYRAC 3 cut(s) 76, 868, 1063
HindII GTYRAC 3 cut(s) 76, 868, 1063
HindIII AAGCTT 3 cut(s) 87, 137, 653
HinfI GANTC 6 cut(s) 218, 323, 328, 915, 1105, 1238
HpaII CCGG 2 cut(s) 627, 927
HphI GGTGA 4 cut(s) 236, 791, 1218, 1331
Hpy166II GTNNAC 4 cut(s) 76, 131, 868, 1063
Hpy188I TCNGA 6 cut(s) 166, 591, 844, 943, 988, 1095
Hpy188III TCNNGA 9 cut(s) 197, 410, 500, 759, 833, 1013, 1029, 1031, 1109
Hpy8I GTNNAC 4 cut(s) 76, 131, 868, 1063
HpyAV CCTTC 3 cut(s) 475, 942, 1167
HpyCH4III ACNGT 4 cut(s) 73, 865, 1118, 1343
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 1 cut(s) 887
HpyF10VI GCNNNNNNNGC 2 cut(s) 638, 1200
HpyF3I CTNAG 2 cut(s) 620, 789
HpySE526I ACGT 1 cut(s) 269
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 9 cut(s) 176, 185, 476, 509, 641, 776, 1016, 1112, 1310
HspAI GCGC 2 cut(s) 350, 1151
Kzo9I GATC 5 cut(s) 3, 199, 361, 676, 943
LguI GCTCTTC 1 cut(s) 252
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 2 cut(s) 427, 1339
MaeI CTAG 4 cut(s) 126, 441, 1083, 1155
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 3 cut(s) 100, 500, 1118
MalI GATC 5 cut(s) 5, 201, 363, 678, 945
MbiI CCGCTC 1 cut(s) 960
MboI GATC 5 cut(s) 3, 199, 361, 676, 943
MboII GAAGA 6 cut(s) 155, 180, 239, 239, 550, 1209
MfeI CAATTG 1 cut(s) 454
MflI RGATCY 2 cut(s) 199, 943
MhlI GDGCHC 3 cut(s) 64, 634, 925
MluCI AATT 7 cut(s) 25, 109, 454, 562, 592, 708, 765
MlyI GAGTC 2 cut(s) 909, 1114
MmeI TCCRAC 1 cut(s) 1266
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 3 cut(s) 12, 116, 693
MslI CAYNNNNRTG 2 cut(s) 777, 1011
MspA1I CMGCKG 2 cut(s) 535, 602
MspI CCGG 2 cut(s) 627, 927
MspR9I CCNGG 8 cut(s) 627, 723, 876, 927, 928, 1056, 1068, 1234
MunI CAATTG 1 cut(s) 454
Mva1269I GAATGC 2 cut(s) 491, 705
MvaI CCWGG 5 cut(s) 723, 876, 1056, 1068, 1234
MwoI GCNNNNNNNGC 2 cut(s) 638, 1200
NciI CCSGG 3 cut(s) 627, 927, 928
NdeII GATC 5 cut(s) 3, 199, 361, 676, 943
NheI GCTAGC 1 cut(s) 440
NlaIII CATG 9 cut(s) 176, 185, 476, 509, 641, 776, 1016, 1112, 1310
NlaIV GGNNCC 3 cut(s) 924, 956, 1178
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 2 cut(s) 176, 185
PaeI GCATGC 1 cut(s) 176
PaeR7I CTCGAG 1 cut(s) 1029
PagI TCATGA 2 cut(s) 1012, 1108
PciI ACATGT 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 1339
PctI GAATGC 2 cut(s) 491, 705
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 4 cut(s) 218, 323, 328, 1238
PflMI CCANNNNNTGG 1 cut(s) 1073
PfoI TCCNGGA 1 cut(s) 874
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 2 cut(s) 909, 1113
PpsI GAGTC 2 cut(s) 909, 1113
PpuMI RGGWCCY 1 cut(s) 1176
PscI ACATGT 1 cut(s) 181
Psp5II RGGWCCY 1 cut(s) 1176
Psp6I CCWGG 5 cut(s) 721, 874, 1054, 1066, 1232
PspGI CCWGG 5 cut(s) 721, 874, 1054, 1066, 1232
PspN4I GGNNCC 3 cut(s) 924, 956, 1178
PspPI GGNCC 6 cut(s) 624, 748, 929, 1049, 1076, 1176
PspPPI RGGWCCY 1 cut(s) 1176
PsuI RGATCY 2 cut(s) 199, 943
PvuII CAGCTG 1 cut(s) 602
RseI CAYNNNNRTG 2 cut(s) 777, 1011
SalI GTCGAC 1 cut(s) 866
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 3 cut(s) 12, 116, 693
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 5 cut(s) 3, 199, 361, 676, 943
Sau96I GGNCC 6 cut(s) 624, 748, 929, 1049, 1076, 1176
SchI GAGTC 2 cut(s) 909, 1114
ScrFI CCNGG 8 cut(s) 627, 723, 876, 927, 928, 1056, 1068, 1234
SduI GDGCHC 3 cut(s) 64, 634, 925
SfaNI GCATC 2 cut(s) 427, 1339
Sfr274I CTCGAG 1 cut(s) 1029
SinI GGWCC 2 cut(s) 748, 1176
SlaI CTCGAG 1 cut(s) 1029
SmaI CCCGGG 1 cut(s) 928
SmiMI CAYNNNNRTG 2 cut(s) 777, 1011
SmlI CTYRAG 4 cut(s) 195, 207, 949, 1029
SmoI CTYRAG 4 cut(s) 195, 207, 949, 1029
SphI GCATGC 1 cut(s) 176
Sse9I AATT 7 cut(s) 25, 109, 454, 562, 592, 708, 765
SsiI CCGC 4 cut(s) 526, 535, 958, 1129
SspMI CTAG 4 cut(s) 126, 441, 1083, 1155
StyD4I CCNGG 8 cut(s) 625, 721, 874, 925, 926, 1054, 1066, 1232
StyI CCWWGG 1 cut(s) 686
TaaI ACNGT 4 cut(s) 73, 865, 1118, 1343
TaiI ACGT 1 cut(s) 272
TaqI TCGA 7 cut(s) 64, 326, 580, 819, 867, 1030, 1136
TasI AATT 7 cut(s) 25, 109, 454, 562, 592, 708, 765
TfiI GAWTC 4 cut(s) 218, 323, 328, 1238
Tru1I TTAA 3 cut(s) 12, 116, 693
Tru9I TTAA 3 cut(s) 12, 116, 693
TscAI CASTG 2 cut(s) 164, 1210
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 1 cut(s) 924
TspGWI ACGGA 1 cut(s) 86
TspMI CCCGGG 1 cut(s) 926
TspRI CASTG 2 cut(s) 164, 1210
Van91I CCANNNNNTGG 1 cut(s) 1073
VpaK11BI GGWCC 2 cut(s) 748, 1176
XagI CCTNNNNNAGG 1 cut(s) 1171
XapI RAATTY 1 cut(s) 592
XceI RCATGY 2 cut(s) 176, 185
XhoI CTCGAG 1 cut(s) 1029
XmaI CCCGGG 1 cut(s) 926
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 4 cut(s) 126, 441, 1083, 1155
ZraI GACGTC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.