Rh2DG618000

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0016905
Physical Location & Seq
Reverse (-)
84823480 .. 84824456
977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG618000.1

Sequence Viewer

Length: 951 bp
ATGGCTTGGGATGAGATGGTGAAGGAAGCCTCAGCTGCTGCAGCACTCGGAGGAGCAGCAAGAAGAGCTCGAAAGCGTTTTGTTGGTGTTCGCCAAAGACCCTCCGGAAGATGGGTGGCAGAGATTAAGGACACAATTCAGAAGATAAGGGTGTGGTTGGGGACATTTGACACAGCTGAGGAAGCAGCCAGGGCCTATGATGAGGCTGCTTGCTTGCTCCGCGGTGCCAATACTAGAACAAATTTCTGGCCTTGCTCCCCATCACCATCTTCAACTCCAGCACTTCCCTCAAAGATCACAAACCTTCTTCTCCAGAGGCTCAAAGCAAGGAACAACAACACCTCTTGTTCTGCTCCATCTGCTGCTCCTTTGCCTGTTAACCACCAACAAAAGCAGTATCAGGAAGAAGCTGCAGCAGAGTTCTCGGAAAACCAATACACTGATTTCCTTAATGATCCAGAAGATTACATCACAAGCAACAATGACAACATCCCTGAGATTAGTGCAAGTAGCATCGACTACATGACATCGAGTTTTGAGTCCTGCTTGACTGAAAAGGAAGAGTACACCACTGCAAGAGAAGCTGAACAACAAATGGACTATGGGAATTTTAGTGAAGTGGCAGAGATTACCTACAGCGGCGGGGATGCAAATTTTGTAGGTGGATCAGAGGAAGATGCGGAAGAAGAAGAAGTGAATGGTCAAGTGGGTGCCATAGACTTCCAATTTGTGGATGATATTGGATCATCAGACTACTACTCTCCTTCTCCTTTCGAGATTGCTGAAGAGATAGAAGAGGCAGTGGAGCCGGAGACCTATGCAGATGAGCCTTCTATGCTAAGAGCAGCCATGAAGAGAATGAAATATGAGAGGAAATTCTCTGCTTCTCTCTATGCCTTCAATGGCATACCCGAATGCTTAAAGAAACACGAGAATGAGGGGAATATCTGA

Protein Analysis

316

Amino Acids

35.16

Weight (kDa)

4.48

Isoelectric Point (pI)

63.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 26 - 75 2.6e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016905)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g47880
malus_domestica MD09G1056000.v1.1 MD17G1053600.v1.1
prunus_persica Prupe.3G263000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0167081
rosa_laevigata RLG00000021695
rosa_multiflora Rmu_sc0010966.1_g000018
rosa_roxburghii Rroxscaffold_2G00083740
rosa_rugosa Rorug02G0528300
rosa_samantha Rh2AG595300 Rh2BG606300 Rh2CG577300 Rh2DG618000
rosa_wichuraiana Rw2G049550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 224, 710
AccB7I CCANNNNNTGG 1 cut(s) 730
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 1 cut(s) 104
AciI CCGC 5 cut(s) 220, 222, 639, 642, 680
AclWI GGATC 3 cut(s) 449, 673, 751
AcsI RAATTY 4 cut(s) 241, 607, 652, 875
AcuI CTGAAG 1 cut(s) 804
AfaI GTAC 1 cut(s) 566
AfiI CCNNNNNNNGG 2 cut(s) 111, 730
AgsI TTSAA 2 cut(s) 273, 901
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 5 cut(s) 35, 68, 176, 410, 584
AluI AGCT 5 cut(s) 35, 68, 176, 410, 584
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 3 cut(s) 449, 673, 751
AlwNI CAGNNNCTG 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 104
AoxI GGCC 2 cut(s) 192, 248
ApoI RAATTY 4 cut(s) 241, 607, 652, 875
ArsI GACNNNNNNTTYG 2 cut(s) 710, 742
AspS9I GGNCC 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 31, 255
BanI GGYRCC 2 cut(s) 224, 710
BanII GRGCYC 1 cut(s) 70
BauI CACGAG 1 cut(s) 929
Bbv12I GWGCWC 1 cut(s) 70
BbvCI CCTCAGC 2 cut(s) 31, 177
BccI CCATC 5 cut(s) 10, 105, 268, 274, 364
BcgI CGANNNNNNTGC 2 cut(s) 405, 439
BciT130I CCWGG 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 806
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 3 cut(s) 39, 411, 634
Bme1390I CCNGG 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 3 cut(s) 226, 712, 807
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 3 cut(s) 522, 637, 667
BpmI CTGGAG 2 cut(s) 261, 296
Bpu10I CCTNAGC 2 cut(s) 31, 177
BsaI GGTCTC 1 cut(s) 806
BsaJI CCNNGG 2 cut(s) 189, 220
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 730
BseAI TCCGGA 1 cut(s) 104
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 2 cut(s) 189, 220
BseGI GGATG 4 cut(s) 16, 489, 652, 739
BseLI CCNNNNNNNGG 2 cut(s) 111, 730
BseMII CTCAG 3 cut(s) 45, 168, 486
BseRI GAGGAG 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 194, 250
BshNI GGYRCC 2 cut(s) 224, 710
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 2 cut(s) 105, 809
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 2 cut(s) 111, 730
BsmAI GTCTC 1 cut(s) 806
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 1 cut(s) 920
BsnI GGCC 2 cut(s) 194, 250
Bso31I GGTCTC 1 cut(s) 806
Bsp1286I GDGCHC 1 cut(s) 70
Bsp13I TCCGGA 1 cut(s) 104
Bsp143I GATC 4 cut(s) 294, 454, 665, 743
BspACI CCGC 5 cut(s) 220, 222, 639, 642, 680
BspANI GGCC 2 cut(s) 194, 250
BspCNI CTCAG 3 cut(s) 44, 169, 487
BspEI TCCGGA 1 cut(s) 104
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 3 cut(s) 226, 712, 807
BspMAI CTGCAG 2 cut(s) 43, 415
BspPI GGATC 3 cut(s) 449, 673, 751
BspQI GCTCTTC 1 cut(s) 58
BspT107I GGYRCC 2 cut(s) 224, 710
BspTNI GGTCTC 1 cut(s) 806
BssECI CCNNGG 2 cut(s) 189, 220
BssMI GATC 4 cut(s) 294, 454, 665, 743
BssSI CACGAG 1 cut(s) 929
Bst2BI CACGAG 1 cut(s) 929
Bst2UI CCWGG 1 cut(s) 190
Bst6I CTCTTC 5 cut(s) 58, 555, 780, 789, 848
BstC8I GCNNGC 2 cut(s) 211, 215
BstDEI CTNAG 4 cut(s) 31, 177, 495, 839
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 4 cut(s) 16, 489, 652, 739
BstFNI CGCG 1 cut(s) 222
BstKTI GATC 4 cut(s) 297, 457, 668, 746
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 4 cut(s) 294, 454, 665, 743
BstMWI GCNNNNNNNGC 9 cut(s) 35, 41, 65, 182, 191, 219, 359, 581, 835
BstNI CCWGG 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 188
BstSFI CTRYAG 3 cut(s) 39, 411, 634
BstUI CGCG 1 cut(s) 222
BsuRI GGCC 2 cut(s) 194, 250
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 4 cut(s) 16, 489, 652, 739
BtsI GCAGTG 2 cut(s) 570, 807
BtsIMutI CAGTG 3 cut(s) 438, 570, 807
Cac8I GCNNGC 2 cut(s) 211, 215
CaiI CAGNNNCTG 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 192
Cfr42I CCGCGG 1 cut(s) 223
Csp6I GTAC 1 cut(s) 565
CviAII CATG 2 cut(s) 523, 850
CviQI GTAC 1 cut(s) 565
DdeI CTNAG 4 cut(s) 31, 177, 495, 839
DpnI GATC 4 cut(s) 296, 456, 667, 745
DpnII GATC 4 cut(s) 294, 454, 665, 743
Eam1104I CTCTTC 5 cut(s) 58, 555, 780, 789, 848
EarI CTCTTC 5 cut(s) 58, 555, 780, 789, 848
Ecl136II GAGCTC 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 806
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 804
EcoICRI GAGCTC 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 70
FaeI CATG 2 cut(s) 526, 853
FaqI GGGAC 1 cut(s) 175
FatI CATG 2 cut(s) 522, 849
FauI CCCGC 1 cut(s) 635
FokI GGATG 4 cut(s) 23, 476, 659, 746
FriOI GRGCYC 1 cut(s) 70
FspBI CTAG 1 cut(s) 234
GsuI CTGGAG 2 cut(s) 261, 296
HaeIII GGCC 2 cut(s) 194, 250
HapII CCGG 2 cut(s) 105, 809
Hin1II CATG 2 cut(s) 526, 853
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 1 cut(s) 539
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 2 cut(s) 105, 809
HphI GGTGA 2 cut(s) 31, 255
Hpy166II GTNNAC 2 cut(s) 379, 567
Hpy188I TCNGA 6 cut(s) 50, 141, 427, 670, 751, 950
Hpy188III TCNNGA 5 cut(s) 105, 313, 401, 458, 775
Hpy8I GTNNAC 2 cut(s) 379, 567
HpyAV CCTTC 5 cut(s) 16, 314, 774, 840, 907
HpyCH4V TGCA 6 cut(s) 41, 413, 506, 575, 650, 821
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 41, 65, 182, 191, 219, 359, 581, 835
HpyF3I CTNAG 4 cut(s) 31, 177, 495, 839
Hsp92II CATG 2 cut(s) 526, 853
Kpn2I TCCGGA 1 cut(s) 104
KspAI GTTAAC 1 cut(s) 379
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 4 cut(s) 294, 454, 665, 743
LguI GCTCTTC 1 cut(s) 58
LmnI GCTCC 6 cut(s) 53, 222, 260, 358, 370, 805
LweI GCATC 3 cut(s) 522, 637, 667
MaeI CTAG 1 cut(s) 234
MalI GATC 4 cut(s) 296, 456, 667, 745
MboI GATC 4 cut(s) 294, 454, 665, 743
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 6 cut(s) 135, 241, 607, 652, 725, 875
MlyI GAGTC 1 cut(s) 548
MroI TCCGGA 1 cut(s) 104
MseI TTAA 4 cut(s) 126, 378, 450, 920
MslI CAYNNNNRTG 1 cut(s) 933
MspA1I CMGCKG 4 cut(s) 35, 176, 222, 639
MspI CCGG 2 cut(s) 105, 809
MspR9I CCNGG 1 cut(s) 190
Mva1269I GAATGC 1 cut(s) 920
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 9 cut(s) 35, 41, 65, 182, 191, 219, 359, 581, 835
NdeII GATC 4 cut(s) 294, 454, 665, 743
NlaIII CATG 2 cut(s) 526, 853
NlaIV GGNNCC 3 cut(s) 226, 712, 807
PciSI GCTCTTC 1 cut(s) 58
PctI GAATGC 1 cut(s) 920
PflMI CCANNNNNTGG 1 cut(s) 730
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
Psp124BI GAGCTC 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 3 cut(s) 226, 712, 807
PspPI GGNCC 1 cut(s) 192
PstI CTGCAG 2 cut(s) 43, 415
PstNI CAGNNNCTG 1 cut(s) 38
PvuII CAGCTG 2 cut(s) 35, 176
RsaI GTAC 1 cut(s) 566
RsaNI GTAC 1 cut(s) 565
RseI CAYNNNNRTG 1 cut(s) 933
SacI GAGCTC 1 cut(s) 70
SacII CCGCGG 1 cut(s) 223
SapI GCTCTTC 1 cut(s) 58
SaqAI TTAA 4 cut(s) 126, 378, 450, 920
Sau3AI GATC 4 cut(s) 294, 454, 665, 743
Sau96I GGNCC 1 cut(s) 192
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 70
SfaNI GCATC 3 cut(s) 522, 637, 667
SfcI CTRYAG 3 cut(s) 39, 411, 634
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 933
Sse9I AATT 6 cut(s) 135, 241, 607, 652, 725, 875
SsiI CCGC 5 cut(s) 220, 222, 639, 642, 680
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 1 cut(s) 188
TaqI TCGA 4 cut(s) 70, 516, 530, 774
TasI AATT 6 cut(s) 135, 241, 607, 652, 725, 875
TatI WGTACW 1 cut(s) 564
TauI GCSGC 1 cut(s) 642
Tru1I TTAA 4 cut(s) 126, 378, 450, 920
Tru9I TTAA 4 cut(s) 126, 378, 450, 920
TscAI CASTG 3 cut(s) 445, 577, 807
TspDTI ATGAA 2 cut(s) 866, 875
TspRI CASTG 3 cut(s) 445, 577, 807
Van91I CCANNNNNTGG 1 cut(s) 730
XapI RAATTY 4 cut(s) 241, 607, 652, 875
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.