Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0015646

Physical Location & Seq

Genomic Coordinates Forward (+)

4086772 .. 4090552

Gene Structure 3781 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG060500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGGAGACTATAACATGGAGAAAACGAAGACTAAGAACTGGAACAAGAGGAAGGCAAAACTCTATCCACAATGAAGATGGATCAAGGTGGGTTGATCTTCTTAATCAGAGGGTATGGTCACAAGAACTTGCTAGCACCCCACCCAGAGGTTGGAACTCCTATAACGCAATCAGCTGGACTATTACAGAGGAAGAGTTCTTGCAAAATGCTGAAATCATCTCCAAGAAGTTACTTCCTCATGGATATGAGTATGTAGTTGTGGACCATCTGTGGTATAGAAGAAATGTGACCGGCGCTAGTGTAGACTCTCTTGGAGTTGATGTAATTGATGAATGGGGGAGACCGATGCCTGACCCAGATAGGTGGCCTAGCTCCAGAGGTGGGAAAGGGTTTACTGAAGTGGCAGACAGAGTACATAAAATGGGTTTGAAGTTTGGAATTAATGTTATGAGAGGAATAAACACACAAGCTGTAAAAGCCAATACCCATATCTTGGATCCTCTCTCGGGGAATGTTTATGATTATTTCAATGGAAAAATTTGGACTGCACAAGATATAGGGATCAAGGAGAGGACTTGTGCTCGGATGCAAAATGGTTTCATGAGTGTGGATACAACCTTGCAAGCTGGAAGGTTCTTCTTGGCAGACCTCTATCAACTGTATGCCAATTGGGGTGTTGATTTTGTGAAACAGGACTGCGTGTTTGGTAATGACTTGGATATGAATGAAATATATCATGCATCATGGCTTTTGAGGAATGATAGACCTATATTGTATTCTGTATCTCCTGGAATTGGAGCAACACCCATGATGGCCAAAAGTGTAAGTGGTGTAGTGAACATGTACAGTATAAACGGGCATAACTGGGATGACTGGGAAGATGTTGTTACCTGTTTTGATGTTGCCAGGGATTTTGCTGCTGCTAATATGATTGGAGCAAACAGCTCGTGGCCTGACTTAGATATGCTTCCCCTGGGATGGCTTAGTGATGCAGGTTCAAATGAAGGTCCACACAGAACAACTAACCTCACATTAGAGGAGCAAAGAACTATGATGACCTTGTGGTGCATGGCCAAGTCTCCTCTCATGTTTGGAGGAGATCTGAGAAAGCTTGATGATACAACTTACAGTATCCTAACAAATCCTACCTTGCTAGAGGTCAATTCTTTTAGCTCAAATAATAAGGAGTTTCCATACATAAAATCTGAAGGACTTCGCTCTTGGGTTGCAACTGGAAGACAACGAGAAATATATGTTGCTTTATTTAATCTGAACTCAAAGAAGATTACTATAACCGCACAAAAATCGGACTTGGCCAAGGCACTTCCTGGGACAACATTGACTACTAGAGCTTCCTGCAAAGGCAAAGAAGTATGGAGCCGACTAGACTTGGGGATTATAGACCACTCCATATCCATTGCTGTAGAAACCCATGGGACTGCACTAGTTGTACTACATTGCAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005994 GO:0005995 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006672 GO:0006677 GO:0006687 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009987 GO:0015925 GO:0016042 GO:0016052 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019377 GO:0030149 GO:0034641 GO:0043603 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044262 GO:0044275 GO:0044424 GO:0044464 GO:0046352 GO:0046466 GO:0046477 GO:0046479 GO:0046514 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1903509

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Melibiase_2

Protein Analysis

488

Amino Acids

55.06

Weight (kDa)

6.28

Isoelectric Point (pI)

31.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	219 - 388	8.9e-10	Alpha galactosidase A
Melibiase_C	PF17801	404 - 484	1.2e-06	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015646)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g05560
rosa_chinensis	RchiOBHm_Chr3g0454341
rosa_laevigata	RLG00000025459
rosa_multiflora	Rmu_sc0000006.1_g000022 Rmu_sc0007209.1_g000014
rosa_roxburghii	Rroxscaffold_165G00437170 Rroxscaffold_6G00425190
rosa_rugosa	Rorug02G0654200 Rorug02G0654300 Rorug02G0654400 Rorug02G0654500
rosa_samantha	Rh3AG058400 Rh3BG060100 Rh3CG059200 Rh3DG060500
rosa_wichuraiana	Rw3G004490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	983
AccB7I	CCANNNNNTGG	2 cut(s)	150, 493
AccI	GTMKAC	1 cut(s)	303
AciI	CCGC	1 cut(s)	1296
AclWI	GGATC	4 cut(s)	88, 491, 504, 569
AcoI	YGGCCR	3 cut(s)	813, 1071, 1314
AcsI	RAATTY	1 cut(s)	537
AcuI	CTGAAG	2 cut(s)	417, 1227
AfaI	GTAC	3 cut(s)	414, 845, 1452
AfiI	CCNNNNNNNGG	7 cut(s)	146, 150, 381, 493, 506, 794, 978
AflIII	ACRYGT	1 cut(s)	840
AgsI	TTSAA	3 cut(s)	430, 529, 999
AhlI	ACTAGT	1 cut(s)	1444
AjnI	CCWGG	4 cut(s)	787, 905, 972, 1327
AluBI	AGCT	8 cut(s)	174, 372, 470, 626, 945, 1111, 1173, 1352
AluI	AGCT	8 cut(s)	174, 372, 470, 626, 945, 1111, 1173, 1352
Alw21I	GWGCWC	1 cut(s)	583
Alw26I	GTCTC	2 cut(s)	334, 1083
AlwI	GGATC	4 cut(s)	88, 491, 504, 569
Ama87I	CYCGRG	1 cut(s)	505
AoxI	GGCC	5 cut(s)	365, 813, 950, 1071, 1314
ApeKI	GCWGC	2 cut(s)	917, 920
ApoI	RAATTY	1 cut(s)	537
ArsI	GACNNNNNNTTYG	2 cut(s)	686, 718
AseI	ATTAAT	1 cut(s)	441
Asp700I	GAANNNNTTC	1 cut(s)	1212
AspLEI	GCGC	1 cut(s)	296
AspS9I	GGNCC	2 cut(s)	262, 1007
AsuNHI	GCTAGC	1 cut(s)	131
AvaI	CYCGRG	1 cut(s)	505
AvaII	GGWCC	2 cut(s)	262, 1007
BalI	TGGCCA	3 cut(s)	815, 1073, 1316
BamHI	GGATCC	1 cut(s)	496
BauI	CACGAG	1 cut(s)	946
BbsI	GAAGAC	2 cut(s)	34, 1243
Bbv12I	GWGCWC	1 cut(s)	583
BbvI	GCAGC	2 cut(s)	904, 907
BccI	CCATC	4 cut(s)	71, 273, 805, 972
BcgI	CGANNNNNNTGC	4 cut(s)	927, 961, 1287, 1321
BciT130I	CCWGG	4 cut(s)	789, 907, 974, 1329
BciVI	GTATCC	2 cut(s)	604, 1142
BcoDI	GTCTC	2 cut(s)	334, 1083
BcuI	ACTAGT	1 cut(s)	1444
BfaI	CTAG	7 cut(s)	132, 297, 369, 1154, 1347, 1385, 1445
BfmI	CTRYAG	1 cut(s)	1422
BfoI	RGCGCY	1 cut(s)	297
BfuAI	ACCTGC	1 cut(s)	983
BfuI	GTATCC	2 cut(s)	604, 1142
BglII	AGATCT	1 cut(s)	1099
BisI	GCNGC	2 cut(s)	918, 921
BlsI	GCNGC	2 cut(s)	919, 922
Bme1390I	CCNGG	4 cut(s)	789, 907, 974, 1329
Bme18I	GGWCC	2 cut(s)	262, 1007
BmeT110I	CYCGRG	1 cut(s)	505
BmgT120I	GGNCC	2 cut(s)	262, 1007
BmiI	GGNNCC	2 cut(s)	498, 1379
BmrFI	CCNGG	4 cut(s)	789, 907, 974, 1329
BmrI	ACTGGG	2 cut(s)	874, 883
BmsI	GCATC	4 cut(s)	336, 576, 749, 979
BmtI	GCTAGC	1 cut(s)	135
BmuI	ACTGGG	2 cut(s)	874, 883
BpiI	GAAGAC	2 cut(s)	34, 1243
BpmI	CTGGAG	1 cut(s)	358
BsaI	GGTCTC	1 cut(s)	334
BsaJI	CCNNGG	6 cut(s)	906, 972, 973, 1317, 1328, 1432
BsaXI	ACNNNNNCTCC	2 cut(s)	306, 336
Bsc4I	CCNNNNNNNGG	7 cut(s)	146, 150, 381, 493, 506, 794, 978
Bse118I	RCCGGY	1 cut(s)	290
Bse1I	ACTGG	4 cut(s)	43, 869, 878, 1237
Bse3DI	GCAATG	2 cut(s)	1416, 1456
BseBI	CCWGG	4 cut(s)	789, 907, 974, 1329
BseDI	CCNNGG	6 cut(s)	906, 972, 973, 1317, 1328, 1432
BseGI	GGATG	3 cut(s)	591, 874, 983
BseLI	CCNNNNNNNGG	7 cut(s)	146, 150, 381, 493, 506, 794, 978
BseMI	GCAATG	2 cut(s)	1416, 1456
BseMII	CTCAG	1 cut(s)	1094
BseNI	ACTGG	4 cut(s)	43, 869, 878, 1237
BseRI	GAGGAG	3 cut(s)	1052, 1071, 1110
BseXI	GCAGC	2 cut(s)	904, 907
BsgI	GTGCAG	2 cut(s)	531, 1425
BshFI	GGCC	5 cut(s)	367, 815, 952, 1073, 1316
BsiHKAI	GWGCWC	1 cut(s)	583
BsiHKCI	CYCGRG	1 cut(s)	505
BsiSI	CCGG	1 cut(s)	291
BslFI	GGGAC	2 cut(s)	1345, 1450
BslI	CCNNNNNNNGG	7 cut(s)	146, 150, 381, 493, 506, 794, 978
BsmAI	GTCTC	2 cut(s)	334, 1083
BsmFI	GGGAC	2 cut(s)	1345, 1450
BsnI	GGCC	5 cut(s)	367, 815, 952, 1073, 1316
Bso31I	GGTCTC	1 cut(s)	334
BsoBI	CYCGRG	1 cut(s)	505
Bsp1286I	GDGCHC	1 cut(s)	583
Bsp1407I	TGTACA	1 cut(s)	843
Bsp143I	GATC	5 cut(s)	80, 94, 496, 561, 1099
Bsp19I	CCATGG	1 cut(s)	1432
BspACI	CCGC	1 cut(s)	1296
BspANI	GGCC	5 cut(s)	367, 815, 952, 1073, 1316
BspCNI	CTCAG	1 cut(s)	1095
BspHI	TCATGA	1 cut(s)	600
BspLI	GGNNCC	2 cut(s)	498, 1379
BspMI	ACCTGC	1 cut(s)	983
BspOI	GCTAGC	1 cut(s)	135
BspPI	GGATC	4 cut(s)	88, 491, 504, 569
BspTNI	GGTCTC	1 cut(s)	334
BsrDI	GCAATG	2 cut(s)	1416, 1456
BsrFI	RCCGGY	1 cut(s)	290
BsrGI	TGTACA	1 cut(s)	843
BsrI	ACTGG	4 cut(s)	43, 869, 878, 1237
BssAI	RCCGGY	1 cut(s)	290
BssECI	CCNNGG	6 cut(s)	906, 972, 973, 1317, 1328, 1432
BssMI	GATC	5 cut(s)	80, 94, 496, 561, 1099
BssSI	CACGAG	1 cut(s)	946
BssT1I	CCWWGG	2 cut(s)	1317, 1432
Bst2BI	CACGAG	1 cut(s)	946
Bst2UI	CCWGG	4 cut(s)	789, 907, 974, 1329
Bst4CI	ACNGT	3 cut(s)	660, 848, 1130
Bst6I	CTCTTC	1 cut(s)	186
BstAUI	TGTACA	1 cut(s)	843
BstC8I	GCNNGC	2 cut(s)	133, 624
BstDEI	CTNAG	4 cut(s)	32, 958, 983, 1103
BstDSI	CCRYGG	1 cut(s)	1432
BstF5I	GGATG	3 cut(s)	591, 874, 983
BstH2I	RGCGCY	1 cut(s)	297
BstHHI	GCGC	1 cut(s)	296
BstKTI	GATC	5 cut(s)	83, 97, 499, 564, 1102
BstMAI	GTCTC	2 cut(s)	334, 1083
BstMBI	GATC	5 cut(s)	80, 94, 496, 561, 1099
BstMWI	GCNNNNNNNGC	1 cut(s)	476
BstNI	CCWGG	4 cut(s)	789, 907, 974, 1329
BstNSI	RCATGY	1 cut(s)	844
BstSCI	CCNGG	4 cut(s)	787, 905, 972, 1327
BstSFI	CTRYAG	1 cut(s)	1422
BstV1I	GCAGC	2 cut(s)	904, 907
BstV2I	GAAGAC	2 cut(s)	34, 1243
BstX2I	RGATCY	2 cut(s)	496, 1099
BstXI	CCANNNNNNTGG	1 cut(s)	363
BstYI	RGATCY	2 cut(s)	496, 1099
BsuI	GTATCC	2 cut(s)	604, 1142
BsuRI	GGCC	5 cut(s)	367, 815, 952, 1073, 1316
BtgI	CCRYGG	1 cut(s)	1432
BtsCI	GGATG	3 cut(s)	591, 874, 983
BveI	ACCTGC	1 cut(s)	983
Cac8I	GCNNGC	2 cut(s)	133, 624
CciI	TCATGA	1 cut(s)	600
CfoI	GCGC	1 cut(s)	296
Cfr10I	RCCGGY	1 cut(s)	290
Cfr13I	GGNCC	2 cut(s)	262, 1007
Csp6I	GTAC	3 cut(s)	413, 844, 1451
CviQI	GTAC	3 cut(s)	413, 844, 1451
DdeI	CTNAG	4 cut(s)	32, 958, 983, 1103
DpnI	GATC	5 cut(s)	82, 96, 498, 563, 1101
DpnII	GATC	5 cut(s)	80, 94, 496, 561, 1099
EaeI	YGGCCR	3 cut(s)	813, 1071, 1314
Eam1104I	CTCTTC	1 cut(s)	186
EarI	CTCTTC	1 cut(s)	186
Eco130I	CCWWGG	2 cut(s)	1317, 1432
Eco31I	GGTCTC	1 cut(s)	334
Eco47I	GGWCC	2 cut(s)	262, 1007
Eco57I	CTGAAG	2 cut(s)	417, 1227
Eco88I	CYCGRG	1 cut(s)	505
EcoRII	CCWGG	4 cut(s)	787, 905, 972, 1327
EcoT14I	CCWWGG	2 cut(s)	1317, 1432
EcoT22I	ATGCAT	1 cut(s)	742
ErhI	CCWWGG	2 cut(s)	1317, 1432
FaqI	GGGAC	2 cut(s)	1345, 1450
FblI	GTMKAC	1 cut(s)	303
Fnu4HI	GCNGC	2 cut(s)	918, 921
FokI	GGATG	3 cut(s)	598, 881, 990
Fsp4HI	GCNGC	2 cut(s)	918, 921
FspBI	CTAG	7 cut(s)	132, 297, 369, 1154, 1347, 1385, 1445
GlaI	GCGC	1 cut(s)	295
GluI	GCNGC	2 cut(s)	918, 921
GsuI	CTGGAG	1 cut(s)	358
HaeII	RGCGCY	1 cut(s)	297
HaeIII	GGCC	5 cut(s)	367, 815, 952, 1073, 1316
HapII	CCGG	1 cut(s)	291
HhaI	GCGC	1 cut(s)	296
Hin6I	GCGC	1 cut(s)	294
HinP1I	GCGC	1 cut(s)	294
HindIII	AAGCTT	1 cut(s)	1109
HinfI	GANTC	1 cut(s)	305
HpaII	CCGG	1 cut(s)	291
Hpy166II	GTNNAC	5 cut(s)	262, 304, 393, 838, 1010
Hpy188I	TCNGA	6 cut(s)	108, 585, 1104, 1207, 1272, 1309
Hpy188III	TCNNGA	2 cut(s)	375, 601
Hpy8I	GTNNAC	5 cut(s)	262, 304, 393, 838, 1010
HpyAV	CCTTC	4 cut(s)	45, 624, 998, 1202
HpyCH4III	ACNGT	3 cut(s)	660, 848, 1130
HpyF10VI	GCNNNNNNNGC	1 cut(s)	476
HpyF3I	CTNAG	4 cut(s)	32, 958, 983, 1103
HspAI	GCGC	1 cut(s)	294
Kzo9I	GATC	5 cut(s)	80, 94, 496, 561, 1099
LmnI	GCTCC	5 cut(s)	377, 797, 935, 1039, 1377
Lsp1109I	GCAGC	2 cut(s)	904, 907
LweI	GCATC	4 cut(s)	336, 576, 749, 979
MaeI	CTAG	7 cut(s)	132, 297, 369, 1154, 1347, 1385, 1445
MaeIII	GTNAC	4 cut(s)	117, 228, 286, 886
MalI	GATC	5 cut(s)	82, 96, 498, 563, 1101
MboI	GATC	5 cut(s)	80, 94, 496, 561, 1099
MboII	GAAGA	9 cut(s)	39, 86, 89, 203, 291, 628, 890, 1248, 1294
MfeI	CAATTG	1 cut(s)	667
MflI	RGATCY	2 cut(s)	496, 1099
MhlI	GDGCHC	1 cut(s)	583
MlsI	TGGCCA	3 cut(s)	815, 1073, 1316
MluCI	AATT	7 cut(s)	324, 438, 537, 667, 792, 1162, 1462
MluNI	TGGCCA	3 cut(s)	815, 1073, 1316
MlyI	GAGTC	1 cut(s)	299
MmeI	TCCRAC	1 cut(s)	131
Mox20I	TGGCCA	3 cut(s)	815, 1073, 1316
Mph1103I	ATGCAT	1 cut(s)	742
MroXI	GAANNNNTTC	1 cut(s)	1212
MscI	TGGCCA	3 cut(s)	815, 1073, 1316
MseI	TTAA	3 cut(s)	102, 441, 1266
MslI	CAYNNNNRTG	2 cut(s)	243, 605
Msp20I	TGGCCA	3 cut(s)	815, 1073, 1316
MspA1I	CMGCKG	1 cut(s)	174
MspI	CCGG	1 cut(s)	291
MspR9I	CCNGG	4 cut(s)	789, 907, 974, 1329
MunI	CAATTG	1 cut(s)	667
MvaI	CCWGG	4 cut(s)	789, 907, 974, 1329
MwoI	GCNNNNNNNGC	1 cut(s)	476
NcoI	CCATGG	1 cut(s)	1432
NdeII	GATC	5 cut(s)	80, 94, 496, 561, 1099
NheI	GCTAGC	1 cut(s)	131
NlaIV	GGNNCC	2 cut(s)	498, 1379
NmuCI	GTSAC	2 cut(s)	117, 286
NsiI	ATGCAT	1 cut(s)	742
NspI	RCATGY	1 cut(s)	844
PagI	TCATGA	1 cut(s)	600
PasI	CCCWGGG	1 cut(s)	973
PciI	ACATGT	1 cut(s)	840
PdmI	GAANNNNTTC	1 cut(s)	1212
PflMI	CCANNNNNTGG	2 cut(s)	150, 493
PfoI	TCCNGGA	1 cut(s)	787
PkrI	GCNGC	2 cut(s)	919, 922
PleI	GAGTC	1 cut(s)	299
PpsI	GAGTC	1 cut(s)	299
PscI	ACATGT	1 cut(s)	840
PshBI	ATTAAT	1 cut(s)	441
Psp6I	CCWGG	4 cut(s)	787, 905, 972, 1327
PspGI	CCWGG	4 cut(s)	787, 905, 972, 1327
PspN4I	GGNNCC	2 cut(s)	498, 1379
PspPI	GGNCC	2 cut(s)	262, 1007
PsuI	RGATCY	2 cut(s)	496, 1099
PvuII	CAGCTG	1 cut(s)	174
RsaI	GTAC	3 cut(s)	414, 845, 1452
RsaNI	GTAC	3 cut(s)	413, 844, 1451
RseI	CAYNNNNRTG	2 cut(s)	243, 605
SaqAI	TTAA	3 cut(s)	102, 441, 1266
SatI	GCNGC	2 cut(s)	918, 921
Sau3AI	GATC	5 cut(s)	80, 94, 496, 561, 1099
Sau96I	GGNCC	2 cut(s)	262, 1007
SchI	GAGTC	1 cut(s)	299
ScrFI	CCNGG	4 cut(s)	789, 907, 974, 1329
SduI	GDGCHC	1 cut(s)	583
SfaNI	GCATC	4 cut(s)	336, 576, 749, 979
SfcI	CTRYAG	1 cut(s)	1422
SinI	GGWCC	2 cut(s)	262, 1007
SmiMI	CAYNNNNRTG	2 cut(s)	243, 605
SpeI	ACTAGT	1 cut(s)	1444
Sse9I	AATT	7 cut(s)	324, 438, 537, 667, 792, 1162, 1462
SsiI	CCGC	1 cut(s)	1296
SspMI	CTAG	7 cut(s)	132, 297, 369, 1154, 1347, 1385, 1445
StyD4I	CCNGG	4 cut(s)	787, 905, 972, 1327
StyI	CCWWGG	2 cut(s)	1317, 1432
TaaI	ACNGT	3 cut(s)	660, 848, 1130
TaqII	GACCGA	1 cut(s)	358
TasI	AATT	7 cut(s)	324, 438, 537, 667, 792, 1162, 1462
TatI	WGTACW	3 cut(s)	412, 843, 1450
Tru1I	TTAA	3 cut(s)	102, 441, 1266
Tru9I	TTAA	3 cut(s)	102, 441, 1266
TseFI	GTSAC	2 cut(s)	117, 286
TseI	GCWGC	2 cut(s)	917, 920
Tsp45I	GTSAC	2 cut(s)	117, 286
TspDTI	ATGAA	6 cut(s)	87, 345, 589, 737, 741, 1017
Van91I	CCANNNNNTGG	2 cut(s)	150, 493
VpaK11BI	GGWCC	2 cut(s)	262, 1007
VspI	ATTAAT	1 cut(s)	441
XapI	RAATTY	1 cut(s)	537
XceI	RCATGY	1 cut(s)	844
XcmI	CCANNNNNNNNNTGG	2 cut(s)	74, 147
XmiI	GTMKAC	1 cut(s)	303
XmnI	GAANNNNTTC	1 cut(s)	1212
XspI	CTAG	7 cut(s)	132, 297, 369, 1154, 1347, 1385, 1445
Zsp2I	ATGCAT	1 cut(s)	742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.